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The map label for this gene is dnaB [H]

Identifier: 197294236

GI number: 197294236

Start: 104355

End: 105545

Strand: Direct

Name: dnaB [H]

Synonym: PAa_0094

Alternate gene names: 197294236

Gene position: 104355-105545 (Clockwise)

Preceding gene: 197294235

Following gene: 197294237

Centisome position: 11.86

GC content: 26.11

Gene sequence:

>1191_bases
ATGATAAATCCAAATGAAGAACATGTTTTATTGAAATTTTTACTTTTAAATCCGCAACAATTTGCTTCTATAATAAACAA
AATTAATATAAATGATTTCCAGAATCCTAAAGTTAAATATATTTACAATCTTTTGTTAGATTTTTACAAACAAAAAAAAA
TTTGGGATTTGAATAAAATGAATTCTTTTTTAACTAAAATTCCTGAATCCAAGCAACAAAACGTTAAGAATTATTTAAAT
TTAATCATTAAAAAAATTCCTTTATGCCAATTATTAGAGGTAGATGATTATATTCAAAGAATCCAAAAAGACAATTCAGA
AAAAGAAATCGAACAAGCTTTTAAAGCAACTTCTCTTTTTGAAACCCCTAAAGATTTAATGCGCAATATTTTAAACAAAA
CTATTTTTAATCCCAGCAAAAACGGATTATTAGGTTTTGATACTGGTTTTGCGCAGTTAAATTACGATACTTTAGGTTTT
CAAAAACAAGAATTAATTATTTTGGGAGCTCGTCCGGGAGTTGGTAAAACAACTTTGATGGTAAATTTAGCTATCAATAT
TATCGCTAAAAATGTTAACTCCAAAATAGTTATTTTTAGTTTGGAGATGAGCAATACTCAATTAATTTTAAAAAGCTTAA
GTTCTGTTTCTAACGTTGACATGCGCCGTTTACAATTGGGAGATGTTAGTGCCAAAGAAAAAGATATAATTCTTGATACT
TCATATACTTTGGAACAATTACAGTTATTTTTTGATGATGAATCTTCTTTAGTTTCTGAAATTGAAATTAAATGTACTCA
GTTTAAGCAAACTCACGGATTAGATATGATTTTTATTGACTATTTACAATTATTGCAAGATAAACCAATGGATAAAATTT
CTAAAAAATTAAAACAATTAGCTAAAAAACTAAATATTGTTGTTTTTTGTCTTTCGCAATTAAATAGAGATTCAGTAAAA
AGTACGACCCCAAGAAGGCCGAGATTAACCGATTTAAAAGATACTGGAAATATTGAACAAGATGCTGATTTAGTGTTGCT
TTTGTATTGTGAAACTAATACGCTGAGAGATAAAGTAACATATTCAAATTATGAATTGATTATTGCTAAAAATAGAAATG
GTATTTCTAATAGAAATTATCAACTGCATTTCAATAATCAAACCCAAACTTTTACAGAAAAGAAAAAATAG

Upstream 100 bases:

>100_bases
TGTTTCGGTTTTTAAAAACAACAAAGAAGCTATAAGTTGTATTTACAGCTTCATAATGTTTATTTCTAATTTATACAATA
TTTTAAAAAAAGGTTATATA

Downstream 100 bases:

>100_bases
AATAGTAATAATAAATTCAAACCCCCTCACCTAATTAACTTGTTTTCATGCTCCCCCAAAAAAAACTAAACTACATTAAT
AAGTAGTTTGGAAAAATGTT

Product: Replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MINPNEEHVLLKFLLLNPQQFASIINKININDFQNPKVKYIYNLLLDFYKQKKIWDLNKMNSFLTKIPESKQQNVKNYLN
LIIKKIPLCQLLEVDDYIQRIQKDNSEKEIEQAFKATSLFETPKDLMRNILNKTIFNPSKNGLLGFDTGFAQLNYDTLGF
QKQELIILGARPGVGKTTLMVNLAINIIAKNVNSKIVIFSLEMSNTQLILKSLSSVSNVDMRRLQLGDVSAKEKDIILDT
SYTLEQLQLFFDDESSLVSEIEIKCTQFKQTHGLDMIFIDYLQLLQDKPMDKISKKLKQLAKKLNIVVFCLSQLNRDSVK
STTPRRPRLTDLKDTGNIEQDADLVLLLYCETNTLRDKVTYSNYELIIAKNRNGISNRNYQLHFNNQTQTFTEKKK

Sequences:

>Translated_396_residues
MINPNEEHVLLKFLLLNPQQFASIINKININDFQNPKVKYIYNLLLDFYKQKKIWDLNKMNSFLTKIPESKQQNVKNYLN
LIIKKIPLCQLLEVDDYIQRIQKDNSEKEIEQAFKATSLFETPKDLMRNILNKTIFNPSKNGLLGFDTGFAQLNYDTLGF
QKQELIILGARPGVGKTTLMVNLAINIIAKNVNSKIVIFSLEMSNTQLILKSLSSVSNVDMRRLQLGDVSAKEKDIILDT
SYTLEQLQLFFDDESSLVSEIEIKCTQFKQTHGLDMIFIDYLQLLQDKPMDKISKKLKQLAKKLNIVVFCLSQLNRDSVK
STTPRRPRLTDLKDTGNIEQDADLVLLLYCETNTLRDKVTYSNYELIIAKNRNGISNRNYQLHFNNQTQTFTEKKK
>Mature_396_residues
MINPNEEHVLLKFLLLNPQQFASIINKININDFQNPKVKYIYNLLLDFYKQKKIWDLNKMNSFLTKIPESKQQNVKNYLN
LIIKKIPLCQLLEVDDYIQRIQKDNSEKEIEQAFKATSLFETPKDLMRNILNKTIFNPSKNGLLGFDTGFAQLNYDTLGF
QKQELIILGARPGVGKTTLMVNLAINIIAKNVNSKIVIFSLEMSNTQLILKSLSSVSNVDMRRLQLGDVSAKEKDIILDT
SYTLEQLQLFFDDESSLVSEIEIKCTQFKQTHGLDMIFIDYLQLLQDKPMDKISKKLKQLAKKLNIVVFCLSQLNRDSVK
STTPRRPRLTDLKDTGNIEQDADLVLLLYCETNTLRDKVTYSNYELIIAKNRNGISNRNYQLHFNNQTQTFTEKKK

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=437, Percent_Identity=29.2906178489703, Blast_Score=164, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 45864; Mature: 45864

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINPNEEHVLLKFLLLNPQQFASIINKININDFQNPKVKYIYNLLLDFYKQKKIWDLNKM
CCCCCHHHEEHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
NSFLTKIPESKQQNVKNYLNLIIKKIPLCQLLEVDDYIQRIQKDNSEKEIEQAFKATSLF
HHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ETPKDLMRNILNKTIFNPSKNGLLGFDTGFAQLNYDTLGFQKQELIILGARPGVGKTTLM
CCHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECCHHCCCCCCEEEEEECCCCCCCCEEE
VNLAINIIAKNVNSKIVIFSLEMSNTQLILKSLSSVSNVDMRRLQLGDVSAKEKDIILDT
EEEEEEHHEECCCCEEEEEEEECCCHHHHHHHHHHHHCCHHHHEEECCCCCCCCCEEEEC
SYTLEQLQLFFDDESSLVSEIEIKCTQFKQTHGLDMIFIDYLQLLQDKPMDKISKKLKQL
CCCHHHHHHEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
AKKLNIVVFCLSQLNRDSVKSTTPRRPRLTDLKDTGNIEQDADLVLLLYCETNTLRDKVT
HHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHCCEE
YSNYELIIAKNRNGISNRNYQLHFNNQTQTFTEKKK
ECCEEEEEEECCCCCCCCCEEEEECCCCCCHHCCCC
>Mature Secondary Structure
MINPNEEHVLLKFLLLNPQQFASIINKININDFQNPKVKYIYNLLLDFYKQKKIWDLNKM
CCCCCHHHEEHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
NSFLTKIPESKQQNVKNYLNLIIKKIPLCQLLEVDDYIQRIQKDNSEKEIEQAFKATSLF
HHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ETPKDLMRNILNKTIFNPSKNGLLGFDTGFAQLNYDTLGFQKQELIILGARPGVGKTTLM
CCHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECCHHCCCCCCEEEEEECCCCCCCCEEE
VNLAINIIAKNVNSKIVIFSLEMSNTQLILKSLSSVSNVDMRRLQLGDVSAKEKDIILDT
EEEEEEHHEECCCCEEEEEEEECCCHHHHHHHHHHHHCCHHHHEEECCCCCCCCCEEEEC
SYTLEQLQLFFDDESSLVSEIEIKCTQFKQTHGLDMIFIDYLQLLQDKPMDKISKKLKQL
CCCHHHHHHEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
AKKLNIVVFCLSQLNRDSVKSTTPRRPRLTDLKDTGNIEQDADLVLLLYCETNTLRDKVT
HHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHCCEE
YSNYELIIAKNRNGISNRNYQLHFNNQTQTFTEKKK
ECCEEEEEEECCCCCCCCCEEEEECCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]