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The map label for this gene is thyA [H]

Identifier: 197294226

GI number: 197294226

Start: 92493

End: 93359

Strand: Direct

Name: thyA [H]

Synonym: PAa_0084

Alternate gene names: 197294226

Gene position: 92493-93359 (Clockwise)

Preceding gene: 197294223

Following gene: 197294227

Centisome position: 10.51

GC content: 28.95

Gene sequence:

>867_bases
ATGAAAACTTATTTAGATTTATGTCGTTTTATTTTAAAAAAAGGTTTTAATAAAGAAGACCGAACGAAAACAGGAACTAA
AAGCGTTTTTGGTTATCAAATGCGTTTCAATCTCCAAGATGGCTTCCCCTTGCTAACTACTAAAAAAATGTATTTCAAAG
CGATTATTCATGAACTTTTATGGTTTTTAAAAGGAGACACGAACATTTCTTATTTAGTCAAAAACAATGTTAATATTTGG
AATGATTGGCCTTATCAAAAATATCAAAAATCGCCTCATTTTAAAAACGAAACTATGGAAGAATTTATTCAAAAAATTAA
AAAAGATAAAGCTTTTGCTGGTTTGCATGGAAATTTAGGTCCTGTTTATGGCAAACAATGGCGTGATTTTAATGGCTTTG
ATCAAATTAAATTTTTAATTTCTGAAATAAAAAATAATCCAGATTCTAGGCGTTTGATTTTGAGTTCTTGGAATCCAGAA
CAATTACATCAAATGGTTTTACCTCCTTGTCATATTTTAATGCAATTTTATGTTTTAGAAGGAAAACTTTCCTTGCAACT
TTATCAAAGGAGTGGCGATGTTTTTTTAGGAGTACCTTTTAATATTGCTTCTTATGCTTTGTTGCTTTTGATGGTTGCTC
AAGTGACAAATTTAGAAGTTGGCGAATTTATTCATACTTTGGGAGACGCACATATTTATCACAACCATTTAAATCAAATC
AAAATTCAAATCCAAAGAACTCCAAAAAAACTTCCCCAAATGATTTTAAACCCTCATATTAAAGATATTGATGGCTTTTC
ATTTGAAGATTTTACTTTAAAAAATTATTTTTGCGATGGAACTTTGAAAGGAGATATTGCTATATGA

Upstream 100 bases:

>100_bases
TTATAAAAATTATGAAATAAAAAAACCAAATATAACATCTTTTTTGGCAAGAAAAACTGAAATTATAATTTATAAAAAAG
ATTTGTAAGGAAAATTTGTT

Downstream 100 bases:

>100_bases
TTAATTTAATCACAGCTTTTGATAGTAATTATCTAATAGGAAGCGATAATAAACTTCCTTGGCATTATCCTCAAGATTTG
CATTTTTTTAAAAAAACAAC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKTYLDLCRFILKKGFNKEDRTKTGTKSVFGYQMRFNLQDGFPLLTTKKMYFKAIIHELLWFLKGDTNISYLVKNNVNIW
NDWPYQKYQKSPHFKNETMEEFIQKIKKDKAFAGLHGNLGPVYGKQWRDFNGFDQIKFLISEIKNNPDSRRLILSSWNPE
QLHQMVLPPCHILMQFYVLEGKLSLQLYQRSGDVFLGVPFNIASYALLLLMVAQVTNLEVGEFIHTLGDAHIYHNHLNQI
KIQIQRTPKKLPQMILNPHIKDIDGFSFEDFTLKNYFCDGTLKGDIAI

Sequences:

>Translated_288_residues
MKTYLDLCRFILKKGFNKEDRTKTGTKSVFGYQMRFNLQDGFPLLTTKKMYFKAIIHELLWFLKGDTNISYLVKNNVNIW
NDWPYQKYQKSPHFKNETMEEFIQKIKKDKAFAGLHGNLGPVYGKQWRDFNGFDQIKFLISEIKNNPDSRRLILSSWNPE
QLHQMVLPPCHILMQFYVLEGKLSLQLYQRSGDVFLGVPFNIASYALLLLMVAQVTNLEVGEFIHTLGDAHIYHNHLNQI
KIQIQRTPKKLPQMILNPHIKDIDGFSFEDFTLKNYFCDGTLKGDIAI
>Mature_288_residues
MKTYLDLCRFILKKGFNKEDRTKTGTKSVFGYQMRFNLQDGFPLLTTKKMYFKAIIHELLWFLKGDTNISYLVKNNVNIW
NDWPYQKYQKSPHFKNETMEEFIQKIKKDKAFAGLHGNLGPVYGKQWRDFNGFDQIKFLISEIKNNPDSRRLILSSWNPE
QLHQMVLPPCHILMQFYVLEGKLSLQLYQRSGDVFLGVPFNIASYALLLLMVAQVTNLEVGEFIHTLGDAHIYHNHLNQI
KIQIQRTPKKLPQMILNPHIKDIDGFSFEDFTLKNYFCDGTLKGDIAI

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=298, Percent_Identity=47.6510067114094, Blast_Score=274, Evalue=7e-74,
Organism=Escherichia coli, GI1789191, Length=293, Percent_Identity=50.1706484641638, Blast_Score=296, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI71993377, Length=299, Percent_Identity=43.8127090301003, Blast_Score=254, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6324648, Length=232, Percent_Identity=42.6724137931034, Blast_Score=186, Evalue=5e-48,
Organism=Drosophila melanogaster, GI17137556, Length=301, Percent_Identity=46.1794019933555, Blast_Score=277, Evalue=5e-75,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 33749; Mature: 33749

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYLDLCRFILKKGFNKEDRTKTGTKSVFGYQMRFNLQDGFPLLTTKKMYFKAIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
WFLKGDTNISYLVKNNVNIWNDWPYQKYQKSPHFKNETMEEFIQKIKKDKAFAGLHGNLG
HHHCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCHHCCCCCCCC
PVYGKQWRDFNGFDQIKFLISEIKNNPDSRRLILSSWNPEQLHQMVLPPCHILMQFYVLE
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCHHHHHHHHHHHC
GKLSLQLYQRSGDVFLGVPFNIASYALLLLMVAQVTNLEVGEFIHTLGDAHIYHNHLNQI
CCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHCCCEE
KIQIQRTPKKLPQMILNPHIKDIDGFSFEDFTLKNYFCDGTLKGDIAI
EEEEECCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKTYLDLCRFILKKGFNKEDRTKTGTKSVFGYQMRFNLQDGFPLLTTKKMYFKAIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
WFLKGDTNISYLVKNNVNIWNDWPYQKYQKSPHFKNETMEEFIQKIKKDKAFAGLHGNLG
HHHCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCHHCCCCCCCC
PVYGKQWRDFNGFDQIKFLISEIKNNPDSRRLILSSWNPEQLHQMVLPPCHILMQFYVLE
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCHHHHHHHHHHHC
GKLSLQLYQRSGDVFLGVPFNIASYALLLLMVAQVTNLEVGEFIHTLGDAHIYHNHLNQI
CCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHCCCEE
KIQIQRTPKKLPQMILNPHIKDIDGFSFEDFTLKNYFCDGTLKGDIAI
EEEEECCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA