Click here to switch to the map view.

The map label for this gene is trmE [H]

Identifier: 197294186

GI number: 197294186

Start: 24023

End: 25384

Strand: Reverse

Name: trmE [H]

Synonym: PAa_0020

Alternate gene names: 197294186

Gene position: 25384-24023 (Counterclockwise)

Preceding gene: 197294187

Following gene: 197294185

Centisome position: 2.88

GC content: 29.44

Gene sequence:

>1362_bases
ATGTTTTTTGATACTATTGCTGCAATCACAACTCCCTTAGGAACAGGTGGGATAAGTGTTATTAGAGTTAGTGGCGCTCA
AGCGATTACAGAAATTAATAAAATTTTTAAAGGTAAAAACTTATTAGAAGCTTTAACGCATACCGTCCATCATGGTTTTA
TTTTAAACCAAGATGAAACTATTTTAGATGAAGTTTTGGTCTCTGTTTTTAAAGCTCCTAATTCTTTTACTGGCGAAAAT
GTTGTAGAAATTAGTGCTCATGGCGGTATTTTAATTACTCAAATGGTGTTAGAACGTATTTTAACTTTAGATCTTAGATT
GGCTTATCCTGGAGAATTTAGTCAAAGAGCTTATATGAATGGTAAAATTGATTTGGTGCAGGCTGAAGCTATCATGGATT
TGATTCATGCCACTAATCAAAATGCTATTAAAATTGCTAATTTAGGGCTCCATAAACACACTTCTCAATTAGTTAGTTCT
TTGCGAGAAAAAATTTTGACTCTTATTGCTCAAATTGAAGTTAATATTGATTATCCTGAATATGATGACACTTTGTTAAT
GACTCAAAGCATTATTGCTCCTCAAGTAAAAAGTTTAATAGAAGAATTAGAGGCTATTTTATTTCATTCTCAAAAAAGTC
GTTATTTAAGAGATGGAATTAAAACTTTAATTATTGGTCGCCCTAATGTTGGTAAATCTAGCTTATTAAATGCTTTATTA
AACGAACCAAAAGCAATTGTTTCTGACATCGCAGGCACTACAAGAGATTTTGTTGATGCTTCTTTTAATTGTCAAGGCAT
TACCCTTAATTTAATTGATACTGCAGGAATTCATCAAACTACAAACCCAATTGAAAAAATCGGTATTTCAAGGACTAAAA
AAATTTTATCATCAGCTGAACTTATTTTATTAGTTTTAGATCAAAGTGAACCTTTAAAGGAAGATGATGAAAAACTATTA
GAATTAACACGTTTAAAACCTCGGATTATTATAGGTAACAAAGCTGATTTAAGTAATGAAAAAACAGTTACTTCTTTTCC
TATCAAAAATTTTATTTCCATTTCTAGTTTAAAGAAAACAGGCTTGGACGAATTACAAAAAGCTATTTTACAAAAGTTTC
AATTAGAACAAATTTCATCTAAAGATTTTAATTCTTTTTCTAACGTGAGGCACATTAATCAAATACAGATAGCACATCAA
GCCTTAAAAGATTTTCAAACTGCTTTCATGGATTCAATGCCAATAGATGTTTATTCCATTGACTTAACCAAGGCTTATCA
AGCTTTAGGAAAAATTATTGGTGATAATCAAGAAAATGATTTAATTAAAGAATTATTTTCTAAATTTTGTTTGGGAAAAT
AA

Upstream 100 bases:

>100_bases
AAAAAGGCATTATAATTTTTTTAATTTTTCAAAAACATTTTTAAATGTTTGCATAATTTTTGTGTAGTTAATTGGTTGTA
TTTCGTGAAATGGTTAAAAA

Downstream 100 bases:

>100_bases
AAATACTATTTTTTTATATTTTTCATGTTGTAATTATTTTTTGTTATTTTCTAATCTCTAAATAATTAAAAGGAATAAAA
AAAGATGAAGTTAATCCAAG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MFFDTIAAITTPLGTGGISVIRVSGAQAITEINKIFKGKNLLEALTHTVHHGFILNQDETILDEVLVSVFKAPNSFTGEN
VVEISAHGGILITQMVLERILTLDLRLAYPGEFSQRAYMNGKIDLVQAEAIMDLIHATNQNAIKIANLGLHKHTSQLVSS
LREKILTLIAQIEVNIDYPEYDDTLLMTQSIIAPQVKSLIEELEAILFHSQKSRYLRDGIKTLIIGRPNVGKSSLLNALL
NEPKAIVSDIAGTTRDFVDASFNCQGITLNLIDTAGIHQTTNPIEKIGISRTKKILSSAELILLVLDQSEPLKEDDEKLL
ELTRLKPRIIIGNKADLSNEKTVTSFPIKNFISISSLKKTGLDELQKAILQKFQLEQISSKDFNSFSNVRHINQIQIAHQ
ALKDFQTAFMDSMPIDVYSIDLTKAYQALGKIIGDNQENDLIKELFSKFCLGK

Sequences:

>Translated_453_residues
MFFDTIAAITTPLGTGGISVIRVSGAQAITEINKIFKGKNLLEALTHTVHHGFILNQDETILDEVLVSVFKAPNSFTGEN
VVEISAHGGILITQMVLERILTLDLRLAYPGEFSQRAYMNGKIDLVQAEAIMDLIHATNQNAIKIANLGLHKHTSQLVSS
LREKILTLIAQIEVNIDYPEYDDTLLMTQSIIAPQVKSLIEELEAILFHSQKSRYLRDGIKTLIIGRPNVGKSSLLNALL
NEPKAIVSDIAGTTRDFVDASFNCQGITLNLIDTAGIHQTTNPIEKIGISRTKKILSSAELILLVLDQSEPLKEDDEKLL
ELTRLKPRIIIGNKADLSNEKTVTSFPIKNFISISSLKKTGLDELQKAILQKFQLEQISSKDFNSFSNVRHINQIQIAHQ
ALKDFQTAFMDSMPIDVYSIDLTKAYQALGKIIGDNQENDLIKELFSKFCLGK
>Mature_453_residues
MFFDTIAAITTPLGTGGISVIRVSGAQAITEINKIFKGKNLLEALTHTVHHGFILNQDETILDEVLVSVFKAPNSFTGEN
VVEISAHGGILITQMVLERILTLDLRLAYPGEFSQRAYMNGKIDLVQAEAIMDLIHATNQNAIKIANLGLHKHTSQLVSS
LREKILTLIAQIEVNIDYPEYDDTLLMTQSIIAPQVKSLIEELEAILFHSQKSRYLRDGIKTLIIGRPNVGKSSLLNALL
NEPKAIVSDIAGTTRDFVDASFNCQGITLNLIDTAGIHQTTNPIEKIGISRTKKILSSAELILLVLDQSEPLKEDDEKLL
ELTRLKPRIIIGNKADLSNEKTVTSFPIKNFISISSLKKTGLDELQKAILQKFQLEQISSKDFNSFSNVRHINQIQIAHQ
ALKDFQTAFMDSMPIDVYSIDLTKAYQALGKIIGDNQENDLIKELFSKFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=469, Percent_Identity=30.4904051172708, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI193082993, Length=455, Percent_Identity=29.6703296703297, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI193082988, Length=498, Percent_Identity=28.714859437751, Blast_Score=169, Evalue=4e-42,
Organism=Escherichia coli, GI2367268, Length=457, Percent_Identity=34.5733041575492, Blast_Score=258, Evalue=8e-70,
Organism=Escherichia coli, GI87082120, Length=127, Percent_Identity=38.5826771653543, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=380, Percent_Identity=29.2105263157895, Blast_Score=137, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=30.8316430020284, Blast_Score=186, Evalue=6e-48,
Organism=Drosophila melanogaster, GI45550813, Length=474, Percent_Identity=29.3248945147679, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50260; Mature: 50260

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFDTIAAITTPLGTGGISVIRVSGAQAITEINKIFKGKNLLEALTHTVHHGFILNQDET
CCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHCCEEECCCHH
ILDEVLVSVFKAPNSFTGENVVEISAHGGILITQMVLERILTLDLRLAYPGEFSQRAYMN
HHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHEEEECCCCCCCHHHCC
GKIDLVQAEAIMDLIHATNQNAIKIANLGLHKHTSQLVSSLREKILTLIAQIEVNIDYPE
CCEEEEHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
YDDTLLMTQSIIAPQVKSLIEELEAILFHSQKSRYLRDGIKTLIIGRPNVGKSSLLNALL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
NEPKAIVSDIAGTTRDFVDASFNCQGITLNLIDTAGIHQTTNPIEKIGISRTKKILSSAE
CCHHHHHHHHCCCHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
LILLVLDQSEPLKEDDEKLLELTRLKPRIIIGNKADLSNEKTVTSFPIKNFISISSLKKT
EEEEEECCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHC
GLDELQKAILQKFQLEQISSKDFNSFSNVRHINQIQIAHQALKDFQTAFMDSMPIDVYSI
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DLTKAYQALGKIIGDNQENDLIKELFSKFCLGK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFFDTIAAITTPLGTGGISVIRVSGAQAITEINKIFKGKNLLEALTHTVHHGFILNQDET
CCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHCCEEECCCHH
ILDEVLVSVFKAPNSFTGENVVEISAHGGILITQMVLERILTLDLRLAYPGEFSQRAYMN
HHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHEEEECCCCCCCHHHCC
GKIDLVQAEAIMDLIHATNQNAIKIANLGLHKHTSQLVSSLREKILTLIAQIEVNIDYPE
CCEEEEHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
YDDTLLMTQSIIAPQVKSLIEELEAILFHSQKSRYLRDGIKTLIIGRPNVGKSSLLNALL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
NEPKAIVSDIAGTTRDFVDASFNCQGITLNLIDTAGIHQTTNPIEKIGISRTKKILSSAE
CCHHHHHHHHCCCHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
LILLVLDQSEPLKEDDEKLLELTRLKPRIIIGNKADLSNEKTVTSFPIKNFISISSLKKT
EEEEEECCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHC
GLDELQKAILQKFQLEQISSKDFNSFSNVRHINQIQIAHQALKDFQTAFMDSMPIDVYSI
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DLTKAYQALGKIIGDNQENDLIKELFSKFCLGK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA