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The map label for this gene is trpE [H]

Identifier: 134302801

GI number: 134302801

Start: 1894230

End: 1895774

Strand: Reverse

Name: trpE [H]

Synonym: FTW_2025

Alternate gene names: 134302801

Gene position: 1895774-1894230 (Counterclockwise)

Preceding gene: 134302802

Following gene: 134302800

Centisome position: 99.86

GC content: 29.71

Gene sequence:

>1545_bases
ATGTCAGTTAAAGAACCAGAAAAATTTTTAGCATCACATATTTATAATATAGATTATCAAGAAGATTTAAATAAATGCTT
TGCTAATTTATGTACAAATAAAAAAAATACTATTTTATTAGAATCAGCAGAAATAGATACAAAAGATAAACTCAAAAGTA
TCTTAGTGCTTAATAGTGCTTTAAGAATAGCTTGTATTGAAAATAAAGTCTTTATAAAATCATTATCTGAAAATGGTAAA
TATGCTCTAAAGGCTATACAGGAAAATATAAAAAAAGATGTTGATTATAAATATGATAATAACCAATTAGTTTTAACATT
TGAGTTCATCAAAGATTATCAACTTAGTGAATCTCAAAAATTAAAAAAACCATCTGTATTTGATGCTTTGAGAATTATAA
AAGATAGTTTTGAAATATATAATGAATTTAGTATTTTCTTAGCAGGATTATTTGCTTATGATGTTGTTGCTAGCTTTGAA
AATATAGGCAAAATTAAGCGTAAAAATAAATGTCCAGATTATGTATTTTACCTTGCTGATACAATACTTGTAAGTGATCA
TCAAGAGCAGAAAACGTTTATTCAAGTTAATAGTTTTGGTCATCAAACAATAACAGATTTAGAGGATAGTGTTGCTGCAA
TTAAGCAAAATATAAATAATGATATAGAGTTAAAAGTTGAAAAGTTTAAAAATATAGAACCTAAAGAATCAATAAATGAT
AGAGATTTTTGTATTGTTGTAGATAAACTTAAATCACATATTATAGATGGTGATATATTTCAAGTAGTGCCTTCAAGAAG
TTTCTTTTTGCCATGTCAAAATTCTTTAGAAGTATATAAAGAACTTAAACGTACTAATCCTAGTCCTTATATGTTTTATA
TGCAAGATGAGGATTTTATTCTTTTTGGAACATCACCAGAGAGTGCATTAAAATATCAAAAGTCAACCAATCAAGTAGAA
ATATATCCTATAGCTGGCACACGTAGAAGAGCTAAAAATTCTGATGGAAGTATCAACGCAGATTTAGATAGTAGAATTGA
ACTAGAGCTTAGATTAGATGCTAAAGAAAATGCGGAACACATGATGCTAGTAGATTTGGCACGTAATGATGTTGCAAGAA
TATCAAAAACAGGTACACGCTATGTAGCAGATCTTCTAAAGGTTGATAGATATAGCCATGTGATGCATTTAGTATCTAGA
GTTGTGGGTCAACTTGCTGATGATTTGGATGCTTTGCATGCTTATCAAGCTTCTATGAATATGGGCACACTAACAGGGGC
TCCAAAAATAAAAGCGATGCAACTAATATCTGAAGTTGAAAAGCAAACAAGAGGTGTTTATGGCGGAGCTATAGGTTATT
TCAATGGTTATGGTGATTTGGATAGTTGTATAGTTATTCGTTCTGCTTATGTTGAAAATGGTGTTGCTGAAATTCAAGCT
GGCGCAGGCGTGGTTTTAGATTCTATACCAATAGCAGAAGCTGATGAGACTAGGACAAAAGCACAAGCTGTAATATCAGC
TATTAAAAACGTACATGGAGTGTAA

Upstream 100 bases:

>100_bases
TTAGTATTTGTACAGTAACTAGATGTTCGAATGCTTTAAAAGATTGTTCTGCAAGGGTAAAAGAAATATTTAGTGAAAAT
ATCAAAAAATAGGTGAATAA

Downstream 100 bases:

>100_bases
CTAATGGCTAATATTATATTTATAGATAATTTTGATTCTTTCTCTTATAATTTAGTTGATGAATTTAGAGTTTTAGGTAA
TCATGTTGAGGTATATAGGA

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSVKEPEKFLASHIYNIDYQEDLNKCFANLCTNKKNTILLESAEIDTKDKLKSILVLNSALRIACIENKVFIKSLSENGK
YALKAIQENIKKDVDYKYDNNQLVLTFEFIKDYQLSESQKLKKPSVFDALRIIKDSFEIYNEFSIFLAGLFAYDVVASFE
NIGKIKRKNKCPDYVFYLADTILVSDHQEQKTFIQVNSFGHQTITDLEDSVAAIKQNINNDIELKVEKFKNIEPKESIND
RDFCIVVDKLKSHIIDGDIFQVVPSRSFFLPCQNSLEVYKELKRTNPSPYMFYMQDEDFILFGTSPESALKYQKSTNQVE
IYPIAGTRRRAKNSDGSINADLDSRIELELRLDAKENAEHMMLVDLARNDVARISKTGTRYVADLLKVDRYSHVMHLVSR
VVGQLADDLDALHAYQASMNMGTLTGAPKIKAMQLISEVEKQTRGVYGGAIGYFNGYGDLDSCIVIRSAYVENGVAEIQA
GAGVVLDSIPIAEADETRTKAQAVISAIKNVHGV

Sequences:

>Translated_514_residues
MSVKEPEKFLASHIYNIDYQEDLNKCFANLCTNKKNTILLESAEIDTKDKLKSILVLNSALRIACIENKVFIKSLSENGK
YALKAIQENIKKDVDYKYDNNQLVLTFEFIKDYQLSESQKLKKPSVFDALRIIKDSFEIYNEFSIFLAGLFAYDVVASFE
NIGKIKRKNKCPDYVFYLADTILVSDHQEQKTFIQVNSFGHQTITDLEDSVAAIKQNINNDIELKVEKFKNIEPKESIND
RDFCIVVDKLKSHIIDGDIFQVVPSRSFFLPCQNSLEVYKELKRTNPSPYMFYMQDEDFILFGTSPESALKYQKSTNQVE
IYPIAGTRRRAKNSDGSINADLDSRIELELRLDAKENAEHMMLVDLARNDVARISKTGTRYVADLLKVDRYSHVMHLVSR
VVGQLADDLDALHAYQASMNMGTLTGAPKIKAMQLISEVEKQTRGVYGGAIGYFNGYGDLDSCIVIRSAYVENGVAEIQA
GAGVVLDSIPIAEADETRTKAQAVISAIKNVHGV
>Mature_513_residues
SVKEPEKFLASHIYNIDYQEDLNKCFANLCTNKKNTILLESAEIDTKDKLKSILVLNSALRIACIENKVFIKSLSENGKY
ALKAIQENIKKDVDYKYDNNQLVLTFEFIKDYQLSESQKLKKPSVFDALRIIKDSFEIYNEFSIFLAGLFAYDVVASFEN
IGKIKRKNKCPDYVFYLADTILVSDHQEQKTFIQVNSFGHQTITDLEDSVAAIKQNINNDIELKVEKFKNIEPKESINDR
DFCIVVDKLKSHIIDGDIFQVVPSRSFFLPCQNSLEVYKELKRTNPSPYMFYMQDEDFILFGTSPESALKYQKSTNQVEI
YPIAGTRRRAKNSDGSINADLDSRIELELRLDAKENAEHMMLVDLARNDVARISKTGTRYVADLLKVDRYSHVMHLVSRV
VGQLADDLDALHAYQASMNMGTLTGAPKIKAMQLISEVEKQTRGVYGGAIGYFNGYGDLDSCIVIRSAYVENGVAEIQAG
AGVVLDSIPIAEADETRTKAQAVISAIKNVHGV

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=499, Percent_Identity=52.3046092184369, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1788114, Length=412, Percent_Identity=27.9126213592233, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1786809, Length=230, Percent_Identity=28.2608695652174, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082077, Length=264, Percent_Identity=28.7878787878788, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320935, Length=380, Percent_Identity=36.5789473684211, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324361, Length=376, Percent_Identity=25, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005257
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 58047; Mature: 57916

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKEPEKFLASHIYNIDYQEDLNKCFANLCTNKKNTILLESAEIDTKDKLKSILVLNSA
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHC
LRIACIENKVFIKSLSENGKYALKAIQENIKKDVDYKYDNNQLVLTFEFIKDYQLSESQK
EEEEEECCHHEEHHHCCCCHHHHHHHHHHHHHCCCCEECCCEEEEEEHHHHCCCCCCHHH
LKKPSVFDALRIIKDSFEIYNEFSIFLAGLFAYDVVASFENIGKIKRKNKCPDYVFYLAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TILVSDHQEQKTFIQVNSFGHQTITDLEDSVAAIKQNINNDIELKVEKFKNIEPKESIND
HHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCC
RDFCIVVDKLKSHIIDGDIFQVVPSRSFFLPCQNSLEVYKELKRTNPSPYMFYMQDEDFI
CCEEEEEHHHHHHCCCCHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCEE
LFGTSPESALKYQKSTNQVEIYPIAGTRRRAKNSDGSINADLDSRIELELRLDAKENAEH
EEECCCHHHHHHHCCCCEEEEEECCCCHHHCCCCCCCEECCCCCEEEEEEEECCCCCCCE
MMLVDLARNDVARISKTGTRYVADLLKVDRYSHVMHLVSRVVGQLADDLDALHAYQASMN
EEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MGTLTGAPKIKAMQLISEVEKQTRGVYGGAIGYFNGYGDLDSCIVIRSAYVENGVAEIQA
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCHHHHCC
GAGVVLDSIPIAEADETRTKAQAVISAIKNVHGV
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVKEPEKFLASHIYNIDYQEDLNKCFANLCTNKKNTILLESAEIDTKDKLKSILVLNSA
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHC
LRIACIENKVFIKSLSENGKYALKAIQENIKKDVDYKYDNNQLVLTFEFIKDYQLSESQK
EEEEEECCHHEEHHHCCCCHHHHHHHHHHHHHCCCCEECCCEEEEEEHHHHCCCCCCHHH
LKKPSVFDALRIIKDSFEIYNEFSIFLAGLFAYDVVASFENIGKIKRKNKCPDYVFYLAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TILVSDHQEQKTFIQVNSFGHQTITDLEDSVAAIKQNINNDIELKVEKFKNIEPKESIND
HHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCC
RDFCIVVDKLKSHIIDGDIFQVVPSRSFFLPCQNSLEVYKELKRTNPSPYMFYMQDEDFI
CCEEEEEHHHHHHCCCCHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCEE
LFGTSPESALKYQKSTNQVEIYPIAGTRRRAKNSDGSINADLDSRIELELRLDAKENAEH
EEECCCHHHHHHHCCCCEEEEEECCCCHHHCCCCCCCEECCCCCEEEEEEEECCCCCCCE
MMLVDLARNDVARISKTGTRYVADLLKVDRYSHVMHLVSRVVGQLADDLDALHAYQASMN
EEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MGTLTGAPKIKAMQLISEVEKQTRGVYGGAIGYFNGYGDLDSCIVIRSAYVENGVAEIQA
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCHHHHCC
GAGVVLDSIPIAEADETRTKAQAVISAIKNVHGV
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1773058; 12620739 [H]