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The map label for this gene is purA [H]

Identifier: 134302691

GI number: 134302691

Start: 1767422

End: 1768708

Strand: Reverse

Name: purA [H]

Synonym: FTW_1884

Alternate gene names: 134302691

Gene position: 1768708-1767422 (Counterclockwise)

Preceding gene: 134302693

Following gene: 134302690

Centisome position: 93.16

GC content: 35.35

Gene sequence:

>1287_bases
ATGTCAAATATCGTAATCGTAGGCGCTCAGTGGGGCGATGAAGGTAAAGGTAAAATAGCAGATACTTTAGCTGAGAAAGC
CGATTTGGTTGTTCGTTATCAAGGTGGTAATAACGCTGGTCATACATTGGTTGTAAATGGTAAGAAGACATTTCTTCATT
TAATACCTTCAGGTGTTTTGCATCAGCATACAAAATGTGTAATTGGTCATGGCGTGGTTTTAGATCCTGTTGCCTTAGAT
GAGGAAATTACACGTCTACAAGCAAAAGGTATAGCAATTTCAGCAGAGAATTTATTTGTTTCTGAGTCTTGTACGATTAT
TACTTCTTATCATAAACTTTTAGATGCAGTTAGAGAAAGTAACACTAGTGAGAAAATAGGTACGACTGGTAAAGGTATTG
GTCCAGCATACGAGGATAAAGTATCGCGTAAAGGTATTAAATTTAAGCATCTATTTGATAAGGATTTATTAAGATCAAGG
TTAGCAATCTCTTTGGCAGAGAAAGAAACTTTATTTAGAGATTTATATAAAGTCGAATATCCAACACTAGAACAAGAATT
TGATAAGCTTTTTGCTTTGGGTCAAAAACTTAAACAGTATGCAGCAGATACTTTTTCAATAATAGATCAAGCAATTGCAG
CGGGTAAGAATGTTGTTTATGAAGGTGCACAAGGTGTGCTTTTAGATGTTGATTATGGTACTTATCCATTTGTGACATCT
TCTAATACTTCAGTGGCGGGAGTATATTCTGGTGCAACCACTGCCGGACATGGTTTAGATCATGTGATTGGGATTACAAA
AGCTTATACTACAAGAGTTGGTGAAGGACCTTTCCCAACAGAGCTTTTTGATGATGTTGGTAAATTTATTCAGCACAAAG
GTGGCGAGATAGGTGTGACAACTGGTAGAATTCGTAGGTGTGGTTGGTTAGATTTACCTCTTTTAAAATACTCAGCAAAA
TGCTCAAACTTAACATCAATAGCCTTAACAAAGGTTGATGTTTTATCTGATATGGACACTCTAAAAGTTTGTATTGGTTA
TAAGTATGAGGGCAAAGAAATATATTGTGCTTATCCAGGAATAGATCTTTACAAGGTAGAGCCAATTCTTGTCGAAATGG
AACCGTTTTCTATAGATGAAACAGTAACAAAAGATAATATGCCAGCTGCTCTTAAGACATATCTTAAGACTATAGAGAAT
CACGTTGGTATTCCAATATCTTCATTAGCTTATGGACCATCTAGAGAACAAATTTTATTCTTTGAGGATTATTTCAAAAA
GGGTTAA

Upstream 100 bases:

>100_bases
TAAAACATTTTTGTTAAACGATATTTTACTAGTAAATTAGTTGCTAATATATTAGTATATTTACTATCAAAATTTTAATC
TCATAGTATTCAAATTTAAT

Downstream 100 bases:

>100_bases
TGTATGACTTATTCTGCAGAAAATACTGAAGTTTATATAACAAGCCAACAACTTGAACAAGCTGTTACTAGATTAGCTGA
ACAAATTAATCAAGACTATT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSNIVIVGAQWGDEGKGKIADTLAEKADLVVRYQGGNNAGHTLVVNGKKTFLHLIPSGVLHQHTKCVIGHGVVLDPVALD
EEITRLQAKGIAISAENLFVSESCTIITSYHKLLDAVRESNTSEKIGTTGKGIGPAYEDKVSRKGIKFKHLFDKDLLRSR
LAISLAEKETLFRDLYKVEYPTLEQEFDKLFALGQKLKQYAADTFSIIDQAIAAGKNVVYEGAQGVLLDVDYGTYPFVTS
SNTSVAGVYSGATTAGHGLDHVIGITKAYTTRVGEGPFPTELFDDVGKFIQHKGGEIGVTTGRIRRCGWLDLPLLKYSAK
CSNLTSIALTKVDVLSDMDTLKVCIGYKYEGKEIYCAYPGIDLYKVEPILVEMEPFSIDETVTKDNMPAALKTYLKTIEN
HVGIPISSLAYGPSREQILFFEDYFKKG

Sequences:

>Translated_428_residues
MSNIVIVGAQWGDEGKGKIADTLAEKADLVVRYQGGNNAGHTLVVNGKKTFLHLIPSGVLHQHTKCVIGHGVVLDPVALD
EEITRLQAKGIAISAENLFVSESCTIITSYHKLLDAVRESNTSEKIGTTGKGIGPAYEDKVSRKGIKFKHLFDKDLLRSR
LAISLAEKETLFRDLYKVEYPTLEQEFDKLFALGQKLKQYAADTFSIIDQAIAAGKNVVYEGAQGVLLDVDYGTYPFVTS
SNTSVAGVYSGATTAGHGLDHVIGITKAYTTRVGEGPFPTELFDDVGKFIQHKGGEIGVTTGRIRRCGWLDLPLLKYSAK
CSNLTSIALTKVDVLSDMDTLKVCIGYKYEGKEIYCAYPGIDLYKVEPILVEMEPFSIDETVTKDNMPAALKTYLKTIEN
HVGIPISSLAYGPSREQILFFEDYFKKG
>Mature_427_residues
SNIVIVGAQWGDEGKGKIADTLAEKADLVVRYQGGNNAGHTLVVNGKKTFLHLIPSGVLHQHTKCVIGHGVVLDPVALDE
EITRLQAKGIAISAENLFVSESCTIITSYHKLLDAVRESNTSEKIGTTGKGIGPAYEDKVSRKGIKFKHLFDKDLLRSRL
AISLAEKETLFRDLYKVEYPTLEQEFDKLFALGQKLKQYAADTFSIIDQAIAAGKNVVYEGAQGVLLDVDYGTYPFVTSS
NTSVAGVYSGATTAGHGLDHVIGITKAYTTRVGEGPFPTELFDDVGKFIQHKGGEIGVTTGRIRRCGWLDLPLLKYSAKC
SNLTSIALTKVDVLSDMDTLKVCIGYKYEGKEIYCAYPGIDLYKVEPILVEMEPFSIDETVTKDNMPAALKTYLKTIENH
VGIPISSLAYGPSREQILFFEDYFKKG

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=428, Percent_Identity=38.785046728972, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI34577063, Length=433, Percent_Identity=39.4919168591224, Blast_Score=291, Evalue=7e-79,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=36.8421052631579, Blast_Score=258, Evalue=9e-69,
Organism=Escherichia coli, GI1790620, Length=429, Percent_Identity=45.6876456876457, Blast_Score=381, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25146555, Length=436, Percent_Identity=40.1376146788991, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI25146553, Length=436, Percent_Identity=40.1376146788991, Blast_Score=289, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6324109, Length=433, Percent_Identity=40.1847575057737, Blast_Score=316, Evalue=5e-87,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=39.4299287410926, Blast_Score=315, Evalue=3e-86,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46880; Mature: 46749

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIVIVGAQWGDEGKGKIADTLAEKADLVVRYQGGNNAGHTLVVNGKKTFLHLIPSGVL
CCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCEEEEECCCHHH
HQHTKCVIGHGVVLDPVALDEEITRLQAKGIAISAENLFVSESCTIITSYHKLLDAVRES
HHCCEEEEECCEEECCHHHHHHHHHHHHCCEEEEECCEEEECCCHHHHHHHHHHHHHHHC
NTSEKIGTTGKGIGPAYEDKVSRKGIKFKHLFDKDLLRSRLAISLAEKETLFRDLYKVEY
CCCHHCCCCCCCCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PTLEQEFDKLFALGQKLKQYAADTFSIIDQAIAAGKNVVYEGAQGVLLDVDYGTYPFVTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCEEEC
SNTSVAGVYSGATTAGHGLDHVIGITKAYTTRVGEGPFPTELFDDVGKFIQHKGGEIGVT
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
TGRIRRCGWLDLPLLKYSAKCSNLTSIALTKVDVLSDMDTLKVCIGYKYEGKEIYCAYPG
CCCHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHEEEEEECCCEEEEECCC
IDLYKVEPILVEMEPFSIDETVTKDNMPAALKTYLKTIENHVGIPISSLAYGPSREQILF
CEEEEECEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEE
FEDYFKKG
EHHHHCCC
>Mature Secondary Structure 
SNIVIVGAQWGDEGKGKIADTLAEKADLVVRYQGGNNAGHTLVVNGKKTFLHLIPSGVL
CCEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCEEEEECCCHHH
HQHTKCVIGHGVVLDPVALDEEITRLQAKGIAISAENLFVSESCTIITSYHKLLDAVRES
HHCCEEEEECCEEECCHHHHHHHHHHHHCCEEEEECCEEEECCCHHHHHHHHHHHHHHHC
NTSEKIGTTGKGIGPAYEDKVSRKGIKFKHLFDKDLLRSRLAISLAEKETLFRDLYKVEY
CCCHHCCCCCCCCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PTLEQEFDKLFALGQKLKQYAADTFSIIDQAIAAGKNVVYEGAQGVLLDVDYGTYPFVTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCEEEC
SNTSVAGVYSGATTAGHGLDHVIGITKAYTTRVGEGPFPTELFDDVGKFIQHKGGEIGVT
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
TGRIRRCGWLDLPLLKYSAKCSNLTSIALTKVDVLSDMDTLKVCIGYKYEGKEIYCAYPG
CCCHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHEEEEEECCCEEEEECCC
IDLYKVEPILVEMEPFSIDETVTKDNMPAALKTYLKTIENHVGIPISSLAYGPSREQILF
CEEEEECEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEE
FEDYFKKG
EHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA