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The map label for this gene is leuA [H]

Identifier: 134302678

GI number: 134302678

Start: 1754862

End: 1756235

Strand: Direct

Name: leuA [H]

Synonym: FTW_1869

Alternate gene names: 134302678

Gene position: 1754862-1756235 (Clockwise)

Preceding gene: 134302677

Following gene: 134302679

Centisome position: 92.44

GC content: 35.23

Gene sequence:

>1374_bases
ATGGATAAGCAAAAAATTTATATTTTTGATACGACTCTTAGAGATGGTCAACAATCTCCAGGAGCAGGCATGTCTTTTGA
AGACAATATTGCATACGCGGACCTCGCTGATAAACTAAATATTGATGTATTAGAAGCTGGTTTTCCTTCTGCTAGTAATA
CAGACTTTGAAATAGTAAATACTATCTCAAAGAGGATGGCTGAGAGAAACTCTAATATGATTATAGCAGGACTATGTCAG
TTAAGAAAAAACCAAGTTGAAATAACTATGGATGCCTTACGCCCATCATTAGCAATAGGTAAAGCTAGAGTGCATATGTA
TTTACCAGTAGATCCAAATTTAGCTCAAGCAAGTCTAGGTAGTAAAAATGATAATGAGCAGAATATTAAAAATGTTTATG
AATTAGTTAAACTAGCTAGTGATGAGGGCTTTGAGGTTGGGTTTAGTGCCGAAGGATATTCAAGGCTAGGCAATACTTTT
GATTATGTTACTAATGTTTTTAGAGCTGCTGTAGCTGCTGGGGTTAAAGTTATTAACTGTCCTGACACTATTGGCGGAGC
ATGCGAGCGTGAAAATGATAATTATTTTGTCAAAAATATGGCTAAACATGCTGAAATCATCAAAAAAGAATTCCCTGATA
GAAATATAATCTGGTCAGCACATTGTCATAATGATTTGGGATTAGCTTTAGAAAACTCTATGAATGCTGTTTTTGATGGT
CCAGCACGACAAGTAGAGGGCTGTATCAATGGAGTTGGTGAGAGAGCCGGTAATGCCTCTTTAGAGCAATGTGTGATGTT
TATAAATCTTTTTGGTAAGCAAAAAGACTATCATTACTACAATGATGTTAATATTGCACATTTTAAAACTATTTCTGATT
TTATCGGCAAAAGAATGTTATCAAGACAGCCACATCACCCTATTACTGGTCTAAACTCAGCAAGGCATACTTCTGGTGGA
CATACAAATGCCATACTAAAAAATCCTCTTGCATATCAGCCATTCCATCCAGAATCTGTCGGTAATGAAATTAGCTTTAT
TTTTGGTCCATTATCAGGTGGTAACCATGCTAAGAAAATTATCGAAGAAAATGGTTATATTTGTGATGACAAAGAAAAGG
CTGCTATAGCTCAGCAAATAAAAGATATTTATCATGAGCGTAGAAAAGGTATCACAGATGAAGAGTTAATACAGGCTTAT
AAACAGATTAAATCTCCGATTCATGTATCAGCTATAGATTATGGTAAATCTAATGGTGAAACTTATGTCGAGCTTAAGGG
AAGATTCTTTGGCAATACAGACTATAGAATTACAGGCCGTGGCGAAAACTCTGCTCTTTCAGCATTATTAAAAGGCATTC
AAGAGTATATATAG

Upstream 100 bases:

>100_bases
TCTGACTACACAATAACAAACCAAATAAAAGAAGTCTTTGAAAAATTAAAGCAAGGACAGGTTTATAAAGAAGCTCTAAC
ATACATATAAGGAGCCTAGT

Downstream 100 bases:

>100_bases
TTAATCCGAGAGTATTTTGTTAAACACATAAGAGATAGCATCATAAAATTTTTCAAAACTATTATTCACTTTTCTAAATA
TTTTTTTAAAGTTAGCCCAA

Product: HMGL-like domain-containing protein

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MDKQKIYIFDTTLRDGQQSPGAGMSFEDNIAYADLADKLNIDVLEAGFPSASNTDFEIVNTISKRMAERNSNMIIAGLCQ
LRKNQVEITMDALRPSLAIGKARVHMYLPVDPNLAQASLGSKNDNEQNIKNVYELVKLASDEGFEVGFSAEGYSRLGNTF
DYVTNVFRAAVAAGVKVINCPDTIGGACERENDNYFVKNMAKHAEIIKKEFPDRNIIWSAHCHNDLGLALENSMNAVFDG
PARQVEGCINGVGERAGNASLEQCVMFINLFGKQKDYHYYNDVNIAHFKTISDFIGKRMLSRQPHHPITGLNSARHTSGG
HTNAILKNPLAYQPFHPESVGNEISFIFGPLSGGNHAKKIIEENGYICDDKEKAAIAQQIKDIYHERRKGITDEELIQAY
KQIKSPIHVSAIDYGKSNGETYVELKGRFFGNTDYRITGRGENSALSALLKGIQEYI

Sequences:

>Translated_457_residues
MDKQKIYIFDTTLRDGQQSPGAGMSFEDNIAYADLADKLNIDVLEAGFPSASNTDFEIVNTISKRMAERNSNMIIAGLCQ
LRKNQVEITMDALRPSLAIGKARVHMYLPVDPNLAQASLGSKNDNEQNIKNVYELVKLASDEGFEVGFSAEGYSRLGNTF
DYVTNVFRAAVAAGVKVINCPDTIGGACERENDNYFVKNMAKHAEIIKKEFPDRNIIWSAHCHNDLGLALENSMNAVFDG
PARQVEGCINGVGERAGNASLEQCVMFINLFGKQKDYHYYNDVNIAHFKTISDFIGKRMLSRQPHHPITGLNSARHTSGG
HTNAILKNPLAYQPFHPESVGNEISFIFGPLSGGNHAKKIIEENGYICDDKEKAAIAQQIKDIYHERRKGITDEELIQAY
KQIKSPIHVSAIDYGKSNGETYVELKGRFFGNTDYRITGRGENSALSALLKGIQEYI
>Mature_457_residues
MDKQKIYIFDTTLRDGQQSPGAGMSFEDNIAYADLADKLNIDVLEAGFPSASNTDFEIVNTISKRMAERNSNMIIAGLCQ
LRKNQVEITMDALRPSLAIGKARVHMYLPVDPNLAQASLGSKNDNEQNIKNVYELVKLASDEGFEVGFSAEGYSRLGNTF
DYVTNVFRAAVAAGVKVINCPDTIGGACERENDNYFVKNMAKHAEIIKKEFPDRNIIWSAHCHNDLGLALENSMNAVFDG
PARQVEGCINGVGERAGNASLEQCVMFINLFGKQKDYHYYNDVNIAHFKTISDFIGKRMLSRQPHHPITGLNSARHTSGG
HTNAILKNPLAYQPFHPESVGNEISFIFGPLSGGNHAKKIIEENGYICDDKEKAAIAQQIKDIYHERRKGITDEELIQAY
KQIKSPIHVSAIDYGKSNGETYVELKGRFFGNTDYRITGRGENSALSALLKGIQEYI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=368, Percent_Identity=33.9673913043478, Blast_Score=165, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6320071, Length=404, Percent_Identity=24.2574257425743, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320019, Length=409, Percent_Identity=25.1833740831296, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324682, Length=366, Percent_Identity=22.9508196721311, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 50701; Mature: 50701

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKQKIYIFDTTLRDGQQSPGAGMSFEDNIAYADLADKLNIDVLEAGFPSASNTDFEIVN
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCHHHHH
TISKRMAERNSNMIIAGLCQLRKNQVEITMDALRPSLAIGKARVHMYLPVDPNLAQASLG
HHHHHHHHCCCCEEEEEHHHHCCCCEEEEHHHHCCHHEECEEEEEEEECCCCCHHHHHCC
SKNDNEQNIKNVYELVKLASDEGFEVGFSAEGYSRLGNTFDYVTNVFRAAVAAGVKVINC
CCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHCCHHHHHHHHHHHHHHHCCEEEEC
PDTIGGACERENDNYFVKNMAKHAEIIKKEFPDRNIIWSAHCHNDLGLALENSMNAVFDG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCHHCCC
PARQVEGCINGVGERAGNASLEQCVMFINLFGKQKDYHYYNDVNIAHFKTISDFIGKRML
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHH
SRQPHHPITGLNSARHTSGGHTNAILKNPLAYQPFHPESVGNEISFIFGPLSGGNHAKKI
HCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHCCCCEEEEEECCCCCHHHHHH
IEENGYICDDKEKAAIAQQIKDIYHERRKGITDEELIQAYKQIKSPIHVSAIDYGKSNGE
HHCCCCEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCC
TYVELKGRFFGNTDYRITGRGENSALSALLKGIQEYI
EEEEEECEEECCCCEEEEECCCHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDKQKIYIFDTTLRDGQQSPGAGMSFEDNIAYADLADKLNIDVLEAGFPSASNTDFEIVN
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCHHHHH
TISKRMAERNSNMIIAGLCQLRKNQVEITMDALRPSLAIGKARVHMYLPVDPNLAQASLG
HHHHHHHHCCCCEEEEEHHHHCCCCEEEEHHHHCCHHEECEEEEEEEECCCCCHHHHHCC
SKNDNEQNIKNVYELVKLASDEGFEVGFSAEGYSRLGNTFDYVTNVFRAAVAAGVKVINC
CCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHCCHHHHHHHHHHHHHHHCCEEEEC
PDTIGGACERENDNYFVKNMAKHAEIIKKEFPDRNIIWSAHCHNDLGLALENSMNAVFDG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCHHCCC
PARQVEGCINGVGERAGNASLEQCVMFINLFGKQKDYHYYNDVNIAHFKTISDFIGKRML
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHH
SRQPHHPITGLNSARHTSGGHTNAILKNPLAYQPFHPESVGNEISFIFGPLSGGNHAKKI
HCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHCCCCEEEEEECCCCCHHHHHH
IEENGYICDDKEKAAIAQQIKDIYHERRKGITDEELIQAYKQIKSPIHVSAIDYGKSNGE
HHCCCCEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCC
TYVELKGRFFGNTDYRITGRGENSALSALLKGIQEYI
EEEEEECEEECCCCEEEEECCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA