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The map label for this gene is lpxD2 [H]

Identifier: 134302620

GI number: 134302620

Start: 1690017

End: 1691060

Strand: Direct

Name: lpxD2 [H]

Synonym: FTW_1800

Alternate gene names: 134302620

Gene position: 1690017-1691060 (Clockwise)

Preceding gene: 134302619

Following gene: 134302626

Centisome position: 89.02

GC content: 35.63

Gene sequence:

>1044_bases
TTGCAATACACTCTAAAACAAATATCCGAGCATCTAAATGCAAAAGTAATTAATGAACCAAGTGGTGAAGTTATTATAAC
GGGGCTTAACTACGCTGAACAAGCAAAAGAAAATGATCTAACTCTTATTGATAAACAAGAACATATTAAGCTTTGGCAAA
ATTCAAAAGCTACAGCTGCTATTGTAAGTAAAAAAATTAGTAAAGAATTAGCACAGGTAAATGACAAGCCTCTAATTGTA
GTCAATAATGCTGATTTAGCTATGGCTAAAATATTAGAACTTTTTTCTGTGCCATACCCAGAACAAAATGGTATTCATGA
AAAAGCTATAATAGATCCAACTGCTAAAATTGGTAAAAATGTCTCAATTGGACCCGGCGCATATATCGGTAAAAATGTCG
AGATTGGTGATAATACGATTATCTATGCCAACGTATGTATCTATAACGATGCAAAAGTTGGAACTAACTGTATTATCTGG
CCAAGTGTGACAATTCGTGATAGAACTATTATAGGTCATTTTTGTAGGTTATACTCAAACTGTTCAATCGGATCAAATGG
TTTTGGCTATAGACCGTCTGAAGATGGTAGAACAATTGTTAGGATTCCACACATTGGTAATGTTGTTATCGGTAGCTTTG
TCGATATTGGTTCAAATACTTGTATTAATAATGCTAAATATGGCTCAACTATAATTGGCGATTATACCAAAATTGATAAT
CTTGTTCAGATAGGTCACAATGTAATTATTGGTAAAGGCTGCATGATTTGTGGACAAGCTGGTATCAGTGGTTCAGTAAC
AATTGGTGACGGCGTTATAATTGCCGGTAATGCTGGTATCAAAGATCATACAAATATTGGTAGTGATGCACGTATCGGTG
GTAAAGCTGGAGTTATGTGGGATGTACCTGCTGGTGAAAGCCATATGGGCTATCCCGCTTACAAAGACAGTGAGCTTGCC
AAACAATGGATTGCTATCAGGAAGCTCCCTGAGACTATGAAAAAACTTAAGGCTATTGCTAAAAGCCTCAACATAGATCT
TTAA

Upstream 100 bases:

>100_bases
GTTAGTAATACTGACTTTGGTGTTGGTGACTAAAGCTTACTATCAAAGTATAGCTTAATCTGCTATGATTATTTAATCAC
AATAAATTTGGAGAAAATGT

Downstream 100 bases:

>100_bases
TTAATTACTTTCTATATAAAACCATATCTAAATCAACATTGTTAAATTCTCAAGATATGGTCTAATCAATTCTATCTTTT
AAGGGCTGTTGATTTAAAGC

Product: UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MQYTLKQISEHLNAKVINEPSGEVIITGLNYAEQAKENDLTLIDKQEHIKLWQNSKATAAIVSKKISKELAQVNDKPLIV
VNNADLAMAKILELFSVPYPEQNGIHEKAIIDPTAKIGKNVSIGPGAYIGKNVEIGDNTIIYANVCIYNDAKVGTNCIIW
PSVTIRDRTIIGHFCRLYSNCSIGSNGFGYRPSEDGRTIVRIPHIGNVVIGSFVDIGSNTCINNAKYGSTIIGDYTKIDN
LVQIGHNVIIGKGCMICGQAGISGSVTIGDGVIIAGNAGIKDHTNIGSDARIGGKAGVMWDVPAGESHMGYPAYKDSELA
KQWIAIRKLPETMKKLKAIAKSLNIDL

Sequences:

>Translated_347_residues
MQYTLKQISEHLNAKVINEPSGEVIITGLNYAEQAKENDLTLIDKQEHIKLWQNSKATAAIVSKKISKELAQVNDKPLIV
VNNADLAMAKILELFSVPYPEQNGIHEKAIIDPTAKIGKNVSIGPGAYIGKNVEIGDNTIIYANVCIYNDAKVGTNCIIW
PSVTIRDRTIIGHFCRLYSNCSIGSNGFGYRPSEDGRTIVRIPHIGNVVIGSFVDIGSNTCINNAKYGSTIIGDYTKIDN
LVQIGHNVIIGKGCMICGQAGISGSVTIGDGVIIAGNAGIKDHTNIGSDARIGGKAGVMWDVPAGESHMGYPAYKDSELA
KQWIAIRKLPETMKKLKAIAKSLNIDL
>Mature_347_residues
MQYTLKQISEHLNAKVINEPSGEVIITGLNYAEQAKENDLTLIDKQEHIKLWQNSKATAAIVSKKISKELAQVNDKPLIV
VNNADLAMAKILELFSVPYPEQNGIHEKAIIDPTAKIGKNVSIGPGAYIGKNVEIGDNTIIYANVCIYNDAKVGTNCIIW
PSVTIRDRTIIGHFCRLYSNCSIGSNGFGYRPSEDGRTIVRIPHIGNVVIGSFVDIGSNTCINNAKYGSTIIGDYTKIDN
LVQIGHNVIIGKGCMICGQAGISGSVTIGDGVIIAGNAGIKDHTNIGSDARIGGKAGVMWDVPAGESHMGYPAYKDSELA
KQWIAIRKLPETMKKLKAIAKSLNIDL

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786376, Length=344, Percent_Identity=32.8488372093023, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786378, Length=206, Percent_Identity=26.6990291262136, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573 [H]

Pfam domain/function: PF00132 Hexapep; PF04613 LpxD [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 37415; Mature: 37415

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYTLKQISEHLNAKVINEPSGEVIITGLNYAEQAKENDLTLIDKQEHIKLWQNSKATAA
CCCHHHHHHHHCCEEEEECCCCCEEEEECCHHHHCCCCCCEEEECHHHHHHHCCCCHHHH
IVSKKISKELAQVNDKPLIVVNNADLAMAKILELFSVPYPEQNGIHEKAIIDPTAKIGKN
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHCCC
VSIGPGAYIGKNVEIGDNTIIYANVCIYNDAKVGTNCIIWPSVTIRDRTIIGHFCRLYSN
CCCCCCCEECCEEEECCCEEEEEEEEEEECCCCCCEEEEECCEEEECHHHHHHHHHHHCC
CSIGSNGFGYRPSEDGRTIVRIPHIGNVVIGSFVDIGSNTCINNAKYGSTIIGDYTKIDN
CCCCCCCCCCCCCCCCCEEEEECCCCCEEEEHHEECCCCCCCCCCCCCCEEECCHHHHHH
LVQIGHNVIIGKGCMICGQAGISGSVTIGDGVIIAGNAGIKDHTNIGSDARIGGKAGVMW
HHHHCCEEEEECCEEEEECCCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCEEE
DVPAGESHMGYPAYKDSELAKQWIAIRKLPETMKKLKAIAKSLNIDL
ECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQYTLKQISEHLNAKVINEPSGEVIITGLNYAEQAKENDLTLIDKQEHIKLWQNSKATAA
CCCHHHHHHHHCCEEEEECCCCCEEEEECCHHHHCCCCCCEEEECHHHHHHHCCCCHHHH
IVSKKISKELAQVNDKPLIVVNNADLAMAKILELFSVPYPEQNGIHEKAIIDPTAKIGKN
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHCCC
VSIGPGAYIGKNVEIGDNTIIYANVCIYNDAKVGTNCIIWPSVTIRDRTIIGHFCRLYSN
CCCCCCCEECCEEEECCCEEEEEEEEEEECCCCCCEEEEECCEEEECHHHHHHHHHHHCC
CSIGSNGFGYRPSEDGRTIVRIPHIGNVVIGSFVDIGSNTCINNAKYGSTIIGDYTKIDN
CCCCCCCCCCCCCCCCCEEEEECCCCCEEEEHHEECCCCCCCCCCCCCCEEECCHHHHHH
LVQIGHNVIIGKGCMICGQAGISGSVTIGDGVIIAGNAGIKDHTNIGSDARIGGKAGVMW
HHHHCCEEEEECCEEEEECCCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCEEE
DVPAGESHMGYPAYKDSELAKQWIAIRKLPETMKKLKAIAKSLNIDL
ECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA