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The map label for this gene is folC [H]

Identifier: 134302542

GI number: 134302542

Start: 1616767

End: 1617948

Strand: Reverse

Name: folC [H]

Synonym: FTW_1705

Alternate gene names: 134302542

Gene position: 1617948-1616767 (Counterclockwise)

Preceding gene: 134302543

Following gene: 134302541

Centisome position: 85.22

GC content: 31.98

Gene sequence:

>1182_bases
ATGAGCGTCGAAATTAAAATAGCTAAATTAATGGCTAAGCCTGATGCTCTCTATTGCGATCTTCTTGATTTATCACTAAT
TCTTAGTAGATTTAATTTTGCTAAAAAGTTTAAAGTCATAACTATAGCTGGTACTAATGGTAAAGGTACGACAGTAGCTA
TGTTAGAAGAGCTTTTAGTAACTAATAATAAGAATGTTTTAAGTCACACTTCACCACACGTTTTTAAATTTAATGAAAGA
ATATCATTAAACAAACAGCCAATATGTGATAGTGTTCTTTTGGAGATTTTAGAAAGATTAGAAGAATTGGCTCCTGAATA
TCGTCTCTCATATTATCAAATTGCTTTTTTATGCCTTTGTATCTATTCGCAAAGAGTTGAGCTTGATTATCTAATTTTAG
AAGTTGGTATTGGTGGTAGATTGGATGCAGCAAATATAATTGATGCTGATATAACAGCTATTACAAATATTGACTTTGAT
CATTGTGAGATATTAGGTGATACTCTTGATAAAATAGGTTTTGAGAAAGCTGGAATATCAAGGCCACAAGTTCCACTTTT
TCTAGGTTCACAAATGCCGCAGAGTGTCTATGAATATGCACAAACGATAGGGGCAGTTATTTATCAAAATAGTTATGAGT
ATAGCTCAAGACAGTGTTTTACTCATAGTTATAATATAGCCATGGGGATAGCAGAATATCTTTTTTATAGGATGCAGATA
TCATATATCCCAAATCTTGAGGATATAAGAGCAAGAGCAAGGTTTGCGACTCTTAAGAATGATTCGCTAAATAACAGTTA
TGTTGTTGTTGATGTTGCTCATAATCCTGCTTCAGTCAGACATTTATTTGAGCTATTAGAGAGCAAATTTGCAGGCAAAA
ATATTCGCTATGAGGCAATATTTGGTATCTTAGCAACCAAAGATATACGCGAAGTACTTAATATAGCTAAACAGCATGTC
TATAAGTGGGATGTCATAGATCTTAAATATCTCGACTCAAGAGCAGCTGATCTTGAAAAGATAAAACAAGAATTTAAATT
ACAACAGATAATTCGTGTAGATTATAACAAAGATTTAAGCAGTGTTTATCTAGCAAAAAAAGATACTCTTACGGTAGTGT
TTGGATCTTTTGTATTAGCAGGAGAATTTATAAGACATTATGAAAAGTATACTAGTAAATGA

Upstream 100 bases:

>100_bases
CTAAATTAATAGACAAACTAATGCCTAATCTCACTAAAATAAACTACTCTCAACCTTTAGAGTATAAGTCTGAACAACAA
GCTTAATTTATAATCAAAAA

Downstream 100 bases:

>100_bases
TGAAGAGCAAATGTATCAACTTGCCAAAGAGTATTCTCAACAGCTAAAGCCTGGTCAGATTATCTATTTGTATGGTGATT
TAGGTGCGGGTAAAACTACG

Product: dihydrofolate synthetase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSVEIKIAKLMAKPDALYCDLLDLSLILSRFNFAKKFKVITIAGTNGKGTTVAMLEELLVTNNKNVLSHTSPHVFKFNER
ISLNKQPICDSVLLEILERLEELAPEYRLSYYQIAFLCLCIYSQRVELDYLILEVGIGGRLDAANIIDADITAITNIDFD
HCEILGDTLDKIGFEKAGISRPQVPLFLGSQMPQSVYEYAQTIGAVIYQNSYEYSSRQCFTHSYNIAMGIAEYLFYRMQI
SYIPNLEDIRARARFATLKNDSLNNSYVVVDVAHNPASVRHLFELLESKFAGKNIRYEAIFGILATKDIREVLNIAKQHV
YKWDVIDLKYLDSRAADLEKIKQEFKLQQIIRVDYNKDLSSVYLAKKDTLTVVFGSFVLAGEFIRHYEKYTSK

Sequences:

>Translated_393_residues
MSVEIKIAKLMAKPDALYCDLLDLSLILSRFNFAKKFKVITIAGTNGKGTTVAMLEELLVTNNKNVLSHTSPHVFKFNER
ISLNKQPICDSVLLEILERLEELAPEYRLSYYQIAFLCLCIYSQRVELDYLILEVGIGGRLDAANIIDADITAITNIDFD
HCEILGDTLDKIGFEKAGISRPQVPLFLGSQMPQSVYEYAQTIGAVIYQNSYEYSSRQCFTHSYNIAMGIAEYLFYRMQI
SYIPNLEDIRARARFATLKNDSLNNSYVVVDVAHNPASVRHLFELLESKFAGKNIRYEAIFGILATKDIREVLNIAKQHV
YKWDVIDLKYLDSRAADLEKIKQEFKLQQIIRVDYNKDLSSVYLAKKDTLTVVFGSFVLAGEFIRHYEKYTSK
>Mature_392_residues
SVEIKIAKLMAKPDALYCDLLDLSLILSRFNFAKKFKVITIAGTNGKGTTVAMLEELLVTNNKNVLSHTSPHVFKFNERI
SLNKQPICDSVLLEILERLEELAPEYRLSYYQIAFLCLCIYSQRVELDYLILEVGIGGRLDAANIIDADITAITNIDFDH
CEILGDTLDKIGFEKAGISRPQVPLFLGSQMPQSVYEYAQTIGAVIYQNSYEYSSRQCFTHSYNIAMGIAEYLFYRMQIS
YIPNLEDIRARARFATLKNDSLNNSYVVVDVAHNPASVRHLFELLESKFAGKNIRYEAIFGILATKDIREVLNIAKQHVY
KWDVIDLKYLDSRAADLEKIKQEFKLQQIIRVDYNKDLSSVYLAKKDTLTVVFGSFVLAGEFIRHYEKYTSK

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=189, Percent_Identity=31.2169312169312, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI66932990, Length=189, Percent_Identity=31.2169312169312, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1788654, Length=376, Percent_Identity=30.3191489361702, Blast_Score=151, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17553150, Length=158, Percent_Identity=31.6455696202532, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71984923, Length=158, Percent_Identity=31.6455696202532, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17553148, Length=158, Percent_Identity=31.6455696202532, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323760, Length=408, Percent_Identity=24.7549019607843, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324815, Length=189, Percent_Identity=31.2169312169312, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24641571, Length=190, Percent_Identity=31.0526315789474, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24581568, Length=156, Percent_Identity=28.8461538461538, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 44924; Mature: 44793

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEIKIAKLMAKPDALYCDLLDLSLILSRFNFAKKFKVITIAGTNGKGTTVAMLEELLV
CCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHCHHHEEEEEEEEECCCCCHHHHHHHHHHH
TNNKNVLSHTSPHVFKFNERISLNKQPICDSVLLEILERLEELAPEYRLSYYQIAFLCLC
CCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
IYSQRVELDYLILEVGIGGRLDAANIIDADITAITNIDFDHCEILGDTLDKIGFEKAGIS
HHHCCCCEEEEEEEECCCCCCCCCHHCCCCHHEEECCCHHHHHHHHHHHHHCCCCCCCCC
RPQVPLFLGSQMPQSVYEYAQTIGAVIYQNSYEYSSRQCFTHSYNIAMGIAEYLFYRMQI
CCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
SYIPNLEDIRARARFATLKNDSLNNSYVVVDVAHNPASVRHLFELLESKFAGKNIRYEAI
HCCCCHHHHHHHHHHHEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHH
FGILATKDIREVLNIAKQHVYKWDVIDLKYLDSRAADLEKIKQEFKLQQIIRVDYNKDLS
HHHHHHHHHHHHHHHHHHHCEEECEEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
SVYLAKKDTLTVVFGSFVLAGEFIRHYEKYTSK
HEEEECCCEEEEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVEIKIAKLMAKPDALYCDLLDLSLILSRFNFAKKFKVITIAGTNGKGTTVAMLEELLV
CEEEEEHHHHCCCCHHHHHHHHHHHHHHHHCHHHEEEEEEEEECCCCCHHHHHHHHHHH
TNNKNVLSHTSPHVFKFNERISLNKQPICDSVLLEILERLEELAPEYRLSYYQIAFLCLC
CCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
IYSQRVELDYLILEVGIGGRLDAANIIDADITAITNIDFDHCEILGDTLDKIGFEKAGIS
HHHCCCCEEEEEEEECCCCCCCCCHHCCCCHHEEECCCHHHHHHHHHHHHHCCCCCCCCC
RPQVPLFLGSQMPQSVYEYAQTIGAVIYQNSYEYSSRQCFTHSYNIAMGIAEYLFYRMQI
CCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
SYIPNLEDIRARARFATLKNDSLNNSYVVVDVAHNPASVRHLFELLESKFAGKNIRYEAI
HCCCCHHHHHHHHHHHEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHH
FGILATKDIREVLNIAKQHVYKWDVIDLKYLDSRAADLEKIKQEFKLQQIIRVDYNKDLS
HHHHHHHHHHHHHHHHHHHCEEECEEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
SVYLAKKDTLTVVFGSFVLAGEFIRHYEKYTSK
HEEEECCCEEEEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]