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The map label for this gene is speE [H]

Identifier: 134302487

GI number: 134302487

Start: 1546242

End: 1547111

Strand: Reverse

Name: speE [H]

Synonym: FTW_1643

Alternate gene names: 134302487

Gene position: 1547111-1546242 (Counterclockwise)

Preceding gene: 134302488

Following gene: 134302486

Centisome position: 81.49

GC content: 33.1

Gene sequence:

>870_bases
ATGATAGCTAATATTAATAATAAAAAAATATTTCATGAAACTTTGTACCATAGCTATCATCAAAGTATAGCAATTTCTGA
AATATTGTATGAACATAAAACAGATTACCAACATCTAGTAATTTTTAATAATCCAATTTTTGGTAATGTCATGGTCTTGG
ATGGTATTGTACAGACTACTGAAAAAGATGAGTTTATTTATCATGAAATGTTAGTGCATGTACCAGTTATCGCGCATGGG
AATGTTAAAAACATATTAATTATTGGTGGCGGCGATGGTGGTATGCTTAGGGAAGCTCTTAGTCATAAAGCTGTCGAATC
TGTAACACTAGTTGAAATTGACCAAGCTGTTATAGATATGTGCCAAGAGTACTTTCCAGGGCATTCAAAAGGTGCTTTTG
ATCATCCTAAAGCGAAAATAGTTATTCAAGATGGTTGTGAATTTGTCAAAAATCCGCCTAGGAAATATGATTTGATTATT
TGTGATAGTACTGATCCGATTGGTCCAGGAGAGGTTTTATTTACCTCAAAATTTTATAAAGACTGTAAAGAAGCTCTAAA
TCCAGATGGTATAATGGTAACCCAAAATGGAGTGATCTACTTTCAAATAGATGAGTTAAAGAAAACTCTTGAGCGCTTTG
AACCATTATATAAAGATGTTTCTTTTTATACTGCAGCAGTTCCAACATATGTCGGTGGAAGTATGGCATTTGGTTGGGGC
ACTGATGAATTATCCTATCGTAATCACGATATTCAAGTAATTGCACAGAGATTTCTTAAGAGCGGTATTAAAACAAGATA
CTACAATCCAGCTATACATATTGCTGCTTTTGCTTTACCACAATATGTTATAGATACTCTAAAGAAGTAA

Upstream 100 bases:

>100_bases
AACCTAATAAAGCAATAGAAGTTTTAGAAAGTTATTTTAAGCCTAAAACAAATAATGTAAGTAAGTTTAGAAGAGGTGAA
GTAGTCGAGGTAGTAAATTA

Downstream 100 bases:

>100_bases
TAACTTAACCTCATAAGGAGGTAAGATGAACAAAAATTTATTTCTAACAGCTAATCTATCAGAGTATTTTAGCTATAATA
GTTTAGGTCAATTATGTATC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1 [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MIANINNKKIFHETLYHSYHQSIAISEILYEHKTDYQHLVIFNNPIFGNVMVLDGIVQTTEKDEFIYHEMLVHVPVIAHG
NVKNILIIGGGDGGMLREALSHKAVESVTLVEIDQAVIDMCQEYFPGHSKGAFDHPKAKIVIQDGCEFVKNPPRKYDLII
CDSTDPIGPGEVLFTSKFYKDCKEALNPDGIMVTQNGVIYFQIDELKKTLERFEPLYKDVSFYTAAVPTYVGGSMAFGWG
TDELSYRNHDIQVIAQRFLKSGIKTRYYNPAIHIAAFALPQYVIDTLKK

Sequences:

>Translated_289_residues
MIANINNKKIFHETLYHSYHQSIAISEILYEHKTDYQHLVIFNNPIFGNVMVLDGIVQTTEKDEFIYHEMLVHVPVIAHG
NVKNILIIGGGDGGMLREALSHKAVESVTLVEIDQAVIDMCQEYFPGHSKGAFDHPKAKIVIQDGCEFVKNPPRKYDLII
CDSTDPIGPGEVLFTSKFYKDCKEALNPDGIMVTQNGVIYFQIDELKKTLERFEPLYKDVSFYTAAVPTYVGGSMAFGWG
TDELSYRNHDIQVIAQRFLKSGIKTRYYNPAIHIAAFALPQYVIDTLKK
>Mature_289_residues
MIANINNKKIFHETLYHSYHQSIAISEILYEHKTDYQHLVIFNNPIFGNVMVLDGIVQTTEKDEFIYHEMLVHVPVIAHG
NVKNILIIGGGDGGMLREALSHKAVESVTLVEIDQAVIDMCQEYFPGHSKGAFDHPKAKIVIQDGCEFVKNPPRKYDLII
CDSTDPIGPGEVLFTSKFYKDCKEALNPDGIMVTQNGVIYFQIDELKKTLERFEPLYKDVSFYTAAVPTYVGGSMAFGWG
TDELSYRNHDIQVIAQRFLKSGIKTRYYNPAIHIAAFALPQYVIDTLKK

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=280, Percent_Identity=35.7142857142857, Blast_Score=200, Evalue=1e-51,
Organism=Escherichia coli, GI1786312, Length=282, Percent_Identity=55.3191489361702, Blast_Score=343, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI25154106, Length=270, Percent_Identity=41.1111111111111, Blast_Score=201, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI212645616, Length=216, Percent_Identity=43.5185185185185, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323175, Length=274, Percent_Identity=38.3211678832117, Blast_Score=202, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6325326, Length=279, Percent_Identity=36.2007168458781, Blast_Score=189, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24645443, Length=265, Percent_Identity=37.3584905660377, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI21355941, Length=217, Percent_Identity=37.7880184331797, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21358269, Length=220, Percent_Identity=28.6363636363636, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24663238, Length=220, Percent_Identity=28.6363636363636, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32723; Mature: 32723

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIANINNKKIFHETLYHSYHQSIAISEILYEHKTDYQHLVIFNNPIFGNVMVLDGIVQTT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEECHHHHCC
EKDEFIYHEMLVHVPVIAHGNVKNILIIGGGDGGMLREALSHKAVESVTLVEIDQAVIDM
CCHHHHHHHHHHHHCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEHHHHHHHHH
CQEYFPGHSKGAFDHPKAKIVIQDGCEFVKNPPRKYDLIICDSTDPIGPGEVLFTSKFYK
HHHHCCCCCCCCCCCCCEEEEEECCHHHHHCCCCCEEEEEECCCCCCCCCCEEEHHHHHH
DCKEALNPDGIMVTQNGVIYFQIDELKKTLERFEPLYKDVSFYTAAVPTYVGGSMAFGWG
HHHHHCCCCCEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
TDELSYRNHDIQVIAQRFLKSGIKTRYYNPAIHIAAFALPQYVIDTLKK
CCCCCCCCCHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIANINNKKIFHETLYHSYHQSIAISEILYEHKTDYQHLVIFNNPIFGNVMVLDGIVQTT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEECHHHHCC
EKDEFIYHEMLVHVPVIAHGNVKNILIIGGGDGGMLREALSHKAVESVTLVEIDQAVIDM
CCHHHHHHHHHHHHCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEHHHHHHHHH
CQEYFPGHSKGAFDHPKAKIVIQDGCEFVKNPPRKYDLIICDSTDPIGPGEVLFTSKFYK
HHHHCCCCCCCCCCCCCEEEEEECCHHHHHCCCCCEEEEEECCCCCCCCCCEEEHHHHHH
DCKEALNPDGIMVTQNGVIYFQIDELKKTLERFEPLYKDVSFYTAAVPTYVGGSMAFGWG
HHHHHCCCCCEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
TDELSYRNHDIQVIAQRFLKSGIKTRYYNPAIHIAAFALPQYVIDTLKK
CCCCCCCCCHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]