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The map label for this gene is 134302485

Identifier: 134302485

GI number: 134302485

Start: 1542739

End: 1543599

Strand: Reverse

Name: 134302485

Synonym: FTW_1638

Alternate gene names: NA

Gene position: 1543599-1542739 (Counterclockwise)

Preceding gene: 134302486

Following gene: 134302482

Centisome position: 81.31

GC content: 34.73

Gene sequence:

>861_bases
ATGGCGAATATAAAAGTTGCAGTTGTCCAATTATCTTTTAATGATAATGAAGCTGAAAATTTAGCAAAACTGGAGAGTAA
AATTATTCAAGCAGCTAAAAATGGTGCAAAAATAATTCTTACTCCAGAGTTACCAAGTTATCTATATTTTTGCAAAAAAC
AAAATTCTAAATATTTTGATTTAGCTAAAACCATTGATGAATCACCAATAGTAAAATTATATAAACTCTTAGCACATAAA
TATAATATTGTTTTGCCTGCTAGTTTTTTTGAGAGAGATGGAAATGCTTGTTATAACTCGATAGCAATGATTGATGCGGA
TGGTTCGATAATGGGTGTATATCGTAAAGCCCATATTCCAGACGGTATTGGTTACCAAGAGAAATATTATTTCTCACCTG
GAAGTGCTGGTTTTAAGGTTTGGGATACTAAATATGCTAAAGTTGGAGTTGGTATTTGCTGGGATCAATGGTTTCCAGAA
GCTGCTAGAGTAATGGCTTTAAAAGGTGCTGAAATTTTATTATATCCAACAGCAATAGGAAGCGAACCACACTTACCAGA
TTACGATTCAAAAGATCATTGGCAAAGAGTGATGCAAGGGCATGCTGCGGCAAATATGTTGCCCGTATTAGCATCAAATA
GATATGCAACTGAGGCAAATGATGATATCACAGCAACTTATTATGGCAGCTCATTCATAACTGATCATACTGGTGATAAA
ATTGCTGAGGCTGACAGAAGCGGTGACGATATACTTTATGCAACATTTGATTTTGCTGAACTACAGCAGCAAAGGTTTTA
TTGGGGATTATTTAGGGATCGTCGCCCTGAGCTTTATGATGAAATTGTTAGAAAGTATTAA

Upstream 100 bases:

>100_bases
GATCGTAGTATTGAACAGCTTAATATTATTGACCTTGTAATTGGTGGAGGAGGAATTCACTGTATTACTATGCAACAACC
GGCTATAAAGGAGATTTGAT

Downstream 100 bases:

>100_bases
ATTGATAAAAATGTTTTTAGTTTAGAGTTCTAACCTGCATAATCTCACCATTTTTTGAGATTTTTATATAGTTACCATCT
TTGAACCAGTCGCCAAGTAC

Product: carbon-nitrogen hydrolase family protein

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MANIKVAVVQLSFNDNEAENLAKLESKIIQAAKNGAKIILTPELPSYLYFCKKQNSKYFDLAKTIDESPIVKLYKLLAHK
YNIVLPASFFERDGNACYNSIAMIDADGSIMGVYRKAHIPDGIGYQEKYYFSPGSAGFKVWDTKYAKVGVGICWDQWFPE
AARVMALKGAEILLYPTAIGSEPHLPDYDSKDHWQRVMQGHAAANMLPVLASNRYATEANDDITATYYGSSFITDHTGDK
IAEADRSGDDILYATFDFAELQQQRFYWGLFRDRRPELYDEIVRKY

Sequences:

>Translated_286_residues
MANIKVAVVQLSFNDNEAENLAKLESKIIQAAKNGAKIILTPELPSYLYFCKKQNSKYFDLAKTIDESPIVKLYKLLAHK
YNIVLPASFFERDGNACYNSIAMIDADGSIMGVYRKAHIPDGIGYQEKYYFSPGSAGFKVWDTKYAKVGVGICWDQWFPE
AARVMALKGAEILLYPTAIGSEPHLPDYDSKDHWQRVMQGHAAANMLPVLASNRYATEANDDITATYYGSSFITDHTGDK
IAEADRSGDDILYATFDFAELQQQRFYWGLFRDRRPELYDEIVRKY
>Mature_285_residues
ANIKVAVVQLSFNDNEAENLAKLESKIIQAAKNGAKIILTPELPSYLYFCKKQNSKYFDLAKTIDESPIVKLYKLLAHKY
NIVLPASFFERDGNACYNSIAMIDADGSIMGVYRKAHIPDGIGYQEKYYFSPGSAGFKVWDTKYAKVGVGICWDQWFPEA
ARVMALKGAEILLYPTAIGSEPHLPDYDSKDHWQRVMQGHAAANMLPVLASNRYATEANDDITATYYGSSFITDHTGDKI
AEADRSGDDILYATFDFAELQQQRFYWGLFRDRRPELYDEIVRKY

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=293, Percent_Identity=30.0341296928328, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI7706509, Length=302, Percent_Identity=26.8211920529801, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI5031947, Length=288, Percent_Identity=25.6944444444444, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI297632350, Length=288, Percent_Identity=25.6944444444444, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI297632348, Length=288, Percent_Identity=25.6944444444444, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI297632346, Length=203, Percent_Identity=26.6009852216749, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17533173, Length=304, Percent_Identity=26.3157894736842, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17556280, Length=283, Percent_Identity=25.0883392226148, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323383, Length=292, Percent_Identity=30.1369863013699, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322335, Length=316, Percent_Identity=25.9493670886076, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21358471, Length=282, Percent_Identity=29.0780141843972, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21355835, Length=287, Percent_Identity=26.8292682926829, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17933642, Length=279, Percent_Identity=23.2974910394265, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32430; Mature: 32299

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIKVAVVQLSFNDNEAENLAKLESKIIQAAKNGAKIILTPELPSYLYFCKKQNSKYFD
CCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCCCEEH
LAKTIDESPIVKLYKLLAHKYNIVLPASFFERDGNACYNSIAMIDADGSIMGVYRKAHIP
HHHHCCCCHHHHHHHHHHHHCCEEEEHHHHHCCCCHHHCEEEEEECCCCEEEEEEECCCC
DGIGYQEKYYFSPGSAGFKVWDTKYAKVGVGICWDQWFPEAARVMALKGAEILLYPTAIG
CCCCCCCEEEECCCCCCEEEECCEEEEEECEEEHHHCCHHHHHEEEECCCEEEEEEECCC
SEPHLPDYDSKDHWQRVMQGHAAANMLPVLASNRYATEANDDITATYYGSSFITDHTGDK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEECCCCCH
IAEADRSGDDILYATFDFAELQQQRFYWGLFRDRRPELYDEIVRKY
HHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
ANIKVAVVQLSFNDNEAENLAKLESKIIQAAKNGAKIILTPELPSYLYFCKKQNSKYFD
CCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCCCEEH
LAKTIDESPIVKLYKLLAHKYNIVLPASFFERDGNACYNSIAMIDADGSIMGVYRKAHIP
HHHHCCCCHHHHHHHHHHHHCCEEEEHHHHHCCCCHHHCEEEEEECCCCEEEEEEECCCC
DGIGYQEKYYFSPGSAGFKVWDTKYAKVGVGICWDQWFPEAARVMALKGAEILLYPTAIG
CCCCCCCEEEECCCCCCEEEECCEEEEEECEEEHHHCCHHHHHEEEECCCEEEEEEECCC
SEPHLPDYDSKDHWQRVMQGHAAANMLPVLASNRYATEANDDITATYYGSSFITDHTGDK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEECCCCCH
IAEADRSGDDILYATFDFAELQQQRFYWGLFRDRRPELYDEIVRKY
HHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]