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The map label for this gene is pyrE [H]

Identifier: 134302483

GI number: 134302483

Start: 1541390

End: 1542016

Strand: Direct

Name: pyrE [H]

Synonym: FTW_1636

Alternate gene names: 134302483

Gene position: 1541390-1542016 (Clockwise)

Preceding gene: 134302481

Following gene: 134302484

Centisome position: 81.19

GC content: 33.65

Gene sequence:

>627_bases
ATGTTTATAGAGTTTGCTCTTAAGAATCAAGTACTTAAATTTGGCGAATTTACGCTTAAGTCAGGACGTATTAGTCCATA
TTTCTTTAATGCTGGGCTATTTAACACAGGCGCTCAACTTGCAACGCTTGCTGATTATTATGCTCAACTGATTATCAAAA
GCGATGTCAAGTACGATATCCTTTTTGGTCCAGCATATAAAGGGATTCCTCTAGTTGCAGCTATTTCTACTGTATTAGCT
CTGAAGTATAATATCGATATGCCTTACGCTTTTGACCGTAAAGAAGCGAAAGATCATGGCGAAGGTGGTGTATTTGTTGG
TGATGATATGACTAATAAAAAAGTATTACTCATAGATGATGTAATGACTGCAGGTACAGCCTTTTATGAATCATACAACA
AGCTAAAAATTATTAATGCCAAAATAGCTGGTGTTGTACTATCGATAGATCGACAAGAAAAAGCTAAAGATAGTGATATT
TCAGCAACTAAGAAAATATCTCAAGATTTCAATATTCCTGTTCTAGCAGTAACTAACTTTGAAAGTATTTTTGAGTATGT
CAAAGAAAATCTTGATGAAACAATGATCGATAAATTTAAACAGTACCGTCAAAAATATGGCTCATAA

Upstream 100 bases:

>100_bases
AATATATTAATAGATATCGTATCTTAACCTATATTTACTTGGTATTATCAAAGTTATTATTTATACTTATAGCAAGTTTA
GTTGTTATTTTATATTCTGT

Downstream 100 bases:

>100_bases
TAAAGATATTTATCTAATTTCGGATCTTCATCTAAACGCTAATCATGCTGAGATGGCTGATCTTTTTAAAAAGTTTTTAG
ATAGCATTACATCTACACAA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MFIEFALKNQVLKFGEFTLKSGRISPYFFNAGLFNTGAQLATLADYYAQLIIKSDVKYDILFGPAYKGIPLVAAISTVLA
LKYNIDMPYAFDRKEAKDHGEGGVFVGDDMTNKKVLLIDDVMTAGTAFYESYNKLKIINAKIAGVVLSIDRQEKAKDSDI
SATKKISQDFNIPVLAVTNFESIFEYVKENLDETMIDKFKQYRQKYGS

Sequences:

>Translated_208_residues
MFIEFALKNQVLKFGEFTLKSGRISPYFFNAGLFNTGAQLATLADYYAQLIIKSDVKYDILFGPAYKGIPLVAAISTVLA
LKYNIDMPYAFDRKEAKDHGEGGVFVGDDMTNKKVLLIDDVMTAGTAFYESYNKLKIINAKIAGVVLSIDRQEKAKDSDI
SATKKISQDFNIPVLAVTNFESIFEYVKENLDETMIDKFKQYRQKYGS
>Mature_208_residues
MFIEFALKNQVLKFGEFTLKSGRISPYFFNAGLFNTGAQLATLADYYAQLIIKSDVKYDILFGPAYKGIPLVAAISTVLA
LKYNIDMPYAFDRKEAKDHGEGGVFVGDDMTNKKVLLIDDVMTAGTAFYESYNKLKIINAKIAGVVLSIDRQEKAKDSDI
SATKKISQDFNIPVLAVTNFESIFEYVKENLDETMIDKFKQYRQKYGS

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=199, Percent_Identity=25.1256281407035, Blast_Score=74, Evalue=7e-14,
Organism=Escherichia coli, GI1790073, Length=207, Percent_Identity=49.2753623188406, Blast_Score=216, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI17508631, Length=185, Percent_Identity=26.4864864864865, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554964, Length=151, Percent_Identity=30.4635761589404, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323530, Length=214, Percent_Identity=47.6635514018692, Blast_Score=204, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6323927, Length=213, Percent_Identity=44.6009389671362, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23388; Mature: 23388

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIEFALKNQVLKFGEFTLKSGRISPYFFNAGLFNTGAQLATLADYYAQLIIKSDVKYDI
CEEEEEHHHHHHHHCCEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
LFGPAYKGIPLVAAISTVLALKYNIDMPYAFDRKEAKDHGEGGVFVGDDMTNKKVLLIDD
EECCCCCCCHHHHHHHHHHHEEECCCCCCCCCCHHHHCCCCCCEEECCCCCCCEEEEEEC
VMTAGTAFYESYNKLKIINAKIAGVVLSIDRQEKAKDSDISATKKISQDFNIPVLAVTNF
HHHHHHHHHHCCCEEEEEEEEEEEEEEEECCHHCCCCCCCHHHHHHHCCCCCCEEEEECH
ESIFEYVKENLDETMIDKFKQYRQKYGS
HHHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFIEFALKNQVLKFGEFTLKSGRISPYFFNAGLFNTGAQLATLADYYAQLIIKSDVKYDI
CEEEEEHHHHHHHHCCEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
LFGPAYKGIPLVAAISTVLALKYNIDMPYAFDRKEAKDHGEGGVFVGDDMTNKKVLLIDD
EECCCCCCCHHHHHHHHHHHEEECCCCCCCCCCHHHHCCCCCCEEECCCCCCCEEEEEEC
VMTAGTAFYESYNKLKIINAKIAGVVLSIDRQEKAKDSDISATKKISQDFNIPVLAVTNF
HHHHHHHHHHCCCEEEEEEEEEEEEEEEECCHHCCCCCCCHHHHHHHCCCCCCEEEEECH
ESIFEYVKENLDETMIDKFKQYRQKYGS
HHHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]