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The map label for this gene is glnS [H]

Identifier: 134302474

GI number: 134302474

Start: 1527172

End: 1528818

Strand: Direct

Name: glnS [H]

Synonym: FTW_1622

Alternate gene names: 134302474

Gene position: 1527172-1528818 (Clockwise)

Preceding gene: 134302469

Following gene: 134302475

Centisome position: 80.44

GC content: 34.18

Gene sequence:

>1647_bases
ATGACTAGTGAAATTAAAGTAAATAAAACTAATTTTATAAAAAATATCATTAAGAAAGACCTCGAAACAAACAAAGTCTC
AAGTATTTTGACGCGTTTTCCTCCTGAGCCTAATGGTTATCTTCATATTGGCCATGCAAAATCAATATGTCTTAATTTTG
GTATTGCACAAGAATTTGATGGTAAGTGTAATCTACGCTTTGATGATACTAATCCTGATAAAGAAGACATTGAGTATATA
AACGCAATCCAAGAAGATGTCGAGTGGCTTGGTTTTAAATGGGAAAATCAACCACGTTTTGCCTCTGAATATTTTGATAA
GATGTATGAGCTAGCTATATTGCTTATAAAAAAAGGCAAAGCTTATGTCTGCGATTTGTCAGCAGAAGAAATACGCGCGT
ACCGTGGCACGCTAAAAGAGCCAGGAAAAAATAGTCCATACAGAGAACGCAGTATCACTGAAAACCTTGAATTATTTGAA
GAGATGAAAAATGGTAAATTTGCTGAAGGTAGTAAAACTTTGAGAGCAAAAATTGATATGTCTTCTGGTAATATCAACCT
TAGAGATCCTGCCTTATACAGAATAAAATTCTCACACCACCCTAAAACTGGTGACAAATGGTGTATTTATCCGATGTATA
CATTTGCTCATCCACTTGAGGATGCAATCGAAGAAATAACTCATTCGTTATGTACTTTGGAATTCCAAGATCAAAGACCA
TTCTATGACTGGGTTATTGAGGAAACAGAATTTAGTATAAAACCACAGCAAATTGAATTTTCAAGACTTAATCTTAATTA
CACAATTACAAGTAAGCGAAAACTAAAATATTTGGTTGATAATAAACTTGTCAATGGCTGGGATGACCCACGTATGCCGA
CTATAAAAGGCTATCGCCGTAGAGGTTATACCCCTGAGTCTATACGCAATTTCTGTGAGATGATAGGAATATCTAAACAA
GATTCTGTTATTGACGTATCGGTTTTAGAAGATGCTGTAAGAGATGATTTAAACAAAAGTGTCTTAAGAAAAAACGTTGT
CCTAGATCCAATAAGAGTAAGCATAAAAGATATGCCAAATCATCATTTAGATGTACCAAACCACCCACAAGATCCAGAAT
TTGGACGCCGCGAGATCACAATATCTTCACAAATATTCATTGAAAGAGATGACTTTGTCTTCAAGCTAGAAAAAGATATG
AAAAAACTTAGTCCTAATGGTAGAGTGCGTTTACTAAATGGCTATGTAATTGAGTGTCAAGAAGTAATTACTGATACTAG
TGGCGAAGTCATAGAACTAAAATGCTCTTATCTACCAGAAACTTTAGGTGGTAAAAAACCAAATGATGGTATCAAGCCTA
ATGGCATTATCCATTGGGTTGATGCTAATAATTGCCTTGATGCTGAAGTTAGGATTTATGATCGCTTATTTAATGATGAA
AATCCAGCAAATGCTGATCGAATTGAAGATGTTTTAAATCCAGACTCATTACAAGTTATCAAAAATGCCAAAGTTGAAAG
ATCTCTTGAAAGTGTCAAAGCTGAAGAACGCTTTCAGTTTAACAGAGTTGGTTATTTTATTGCTGACTTAAAAGATTGCT
CTAACGACACATTAGTATTTAATCATATTGCAACTCTTAGAAACTGA

Upstream 100 bases:

>100_bases
TAGCTAGTATATACTTTTTTTTCAAAAAAATATGTTATTGTTTTTTTATTAAAGTAAACTATTAAATAAAATTTATTTAA
GAACTATTTCTAAGACAAAA

Downstream 100 bases:

>100_bases
TTCAAAGGTAATACACTATAATGTTATCAAACTCAAAGAAAACTATTATATGTGGTTTTTCTTTTTTTTGTATCTGGATA
TTTGGAACTCTAGCCTTATA

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MTSEIKVNKTNFIKNIIKKDLETNKVSSILTRFPPEPNGYLHIGHAKSICLNFGIAQEFDGKCNLRFDDTNPDKEDIEYI
NAIQEDVEWLGFKWENQPRFASEYFDKMYELAILLIKKGKAYVCDLSAEEIRAYRGTLKEPGKNSPYRERSITENLELFE
EMKNGKFAEGSKTLRAKIDMSSGNINLRDPALYRIKFSHHPKTGDKWCIYPMYTFAHPLEDAIEEITHSLCTLEFQDQRP
FYDWVIEETEFSIKPQQIEFSRLNLNYTITSKRKLKYLVDNKLVNGWDDPRMPTIKGYRRRGYTPESIRNFCEMIGISKQ
DSVIDVSVLEDAVRDDLNKSVLRKNVVLDPIRVSIKDMPNHHLDVPNHPQDPEFGRREITISSQIFIERDDFVFKLEKDM
KKLSPNGRVRLLNGYVIECQEVITDTSGEVIELKCSYLPETLGGKKPNDGIKPNGIIHWVDANNCLDAEVRIYDRLFNDE
NPANADRIEDVLNPDSLQVIKNAKVERSLESVKAEERFQFNRVGYFIADLKDCSNDTLVFNHIATLRN

Sequences:

>Translated_548_residues
MTSEIKVNKTNFIKNIIKKDLETNKVSSILTRFPPEPNGYLHIGHAKSICLNFGIAQEFDGKCNLRFDDTNPDKEDIEYI
NAIQEDVEWLGFKWENQPRFASEYFDKMYELAILLIKKGKAYVCDLSAEEIRAYRGTLKEPGKNSPYRERSITENLELFE
EMKNGKFAEGSKTLRAKIDMSSGNINLRDPALYRIKFSHHPKTGDKWCIYPMYTFAHPLEDAIEEITHSLCTLEFQDQRP
FYDWVIEETEFSIKPQQIEFSRLNLNYTITSKRKLKYLVDNKLVNGWDDPRMPTIKGYRRRGYTPESIRNFCEMIGISKQ
DSVIDVSVLEDAVRDDLNKSVLRKNVVLDPIRVSIKDMPNHHLDVPNHPQDPEFGRREITISSQIFIERDDFVFKLEKDM
KKLSPNGRVRLLNGYVIECQEVITDTSGEVIELKCSYLPETLGGKKPNDGIKPNGIIHWVDANNCLDAEVRIYDRLFNDE
NPANADRIEDVLNPDSLQVIKNAKVERSLESVKAEERFQFNRVGYFIADLKDCSNDTLVFNHIATLRN
>Mature_547_residues
TSEIKVNKTNFIKNIIKKDLETNKVSSILTRFPPEPNGYLHIGHAKSICLNFGIAQEFDGKCNLRFDDTNPDKEDIEYIN
AIQEDVEWLGFKWENQPRFASEYFDKMYELAILLIKKGKAYVCDLSAEEIRAYRGTLKEPGKNSPYRERSITENLELFEE
MKNGKFAEGSKTLRAKIDMSSGNINLRDPALYRIKFSHHPKTGDKWCIYPMYTFAHPLEDAIEEITHSLCTLEFQDQRPF
YDWVIEETEFSIKPQQIEFSRLNLNYTITSKRKLKYLVDNKLVNGWDDPRMPTIKGYRRRGYTPESIRNFCEMIGISKQD
SVIDVSVLEDAVRDDLNKSVLRKNVVLDPIRVSIKDMPNHHLDVPNHPQDPEFGRREITISSQIFIERDDFVFKLEKDMK
KLSPNGRVRLLNGYVIECQEVITDTSGEVIELKCSYLPETLGGKKPNDGIKPNGIIHWVDANNCLDAEVRIYDRLFNDEN
PANADRIEDVLNPDSLQVIKNAKVERSLESVKAEERFQFNRVGYFIADLKDCSNDTLVFNHIATLRN

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=548, Percent_Identity=42.5182481751825, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI62241042, Length=380, Percent_Identity=41.5789473684211, Blast_Score=272, Evalue=6e-73,
Organism=Escherichia coli, GI1786895, Length=548, Percent_Identity=54.1970802919708, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI1788743, Length=310, Percent_Identity=26.4516129032258, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17543332, Length=531, Percent_Identity=42.1845574387947, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17511007, Length=514, Percent_Identity=30.9338521400778, Blast_Score=231, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6324742, Length=576, Percent_Identity=38.5416666666667, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321192, Length=523, Percent_Identity=34.6080305927342, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6324540, Length=310, Percent_Identity=25.4838709677419, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17864488, Length=528, Percent_Identity=42.8030303030303, Blast_Score=426, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24649466, Length=430, Percent_Identity=36.9767441860465, Blast_Score=254, Evalue=1e-67,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 63533; Mature: 63402

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSEIKVNKTNFIKNIIKKDLETNKVSSILTRFPPEPNGYLHIGHAKSICLNFGIAQEFD
CCCCEECCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCHHEEHEECCCCCCCC
GKCNLRFDDTNPDKEDIEYINAIQEDVEWLGFKWENQPRFASEYFDKMYELAILLIKKGK
CEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHEEECCC
AYVCDLSAEEIRAYRGTLKEPGKNSPYRERSITENLELFEEMKNGKFAEGSKTLRAKIDM
EEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
SSGNINLRDPALYRIKFSHHPKTGDKWCIYPMYTFAHPLEDAIEEITHSLCTLEFQDQRP
CCCCEEECCCEEEEEEECCCCCCCCCEEEEEHHHHHCHHHHHHHHHHHHHEEEEECCCCC
FYDWVIEETEFSIKPQQIEFSRLNLNYTITSKRKLKYLVDNKLVNGWDDPRMPTIKGYRR
HHHEEEECCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHH
RGYTPESIRNFCEMIGISKQDSVIDVSVLEDAVRDDLNKSVLRKNVVLDPIRVSIKDMPN
CCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCEECCCEEEEECCCC
HHLDVPNHPQDPEFGRREITISSQIFIERDDFVFKLEKDMKKLSPNGRVRLLNGYVIECQ
CCCCCCCCCCCCCCCCEEEEEEEEEEEECCCEEEEEHHHHHHCCCCCCEEEECCEEEEEH
EVITDTSGEVIELKCSYLPETLGGKKPNDGIKPNGIIHWVDANNCLDAEVRIYDRLFNDE
HHHCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCC
NPANADRIEDVLNPDSLQVIKNAKVERSLESVKAEERFQFNRVGYFIADLKDCSNDTLVF
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCCCCEEE
NHIATLRN
EHHHHHCC
>Mature Secondary Structure 
TSEIKVNKTNFIKNIIKKDLETNKVSSILTRFPPEPNGYLHIGHAKSICLNFGIAQEFD
CCCEECCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCHHEEHEECCCCCCCC
GKCNLRFDDTNPDKEDIEYINAIQEDVEWLGFKWENQPRFASEYFDKMYELAILLIKKGK
CEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHEEECCC
AYVCDLSAEEIRAYRGTLKEPGKNSPYRERSITENLELFEEMKNGKFAEGSKTLRAKIDM
EEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
SSGNINLRDPALYRIKFSHHPKTGDKWCIYPMYTFAHPLEDAIEEITHSLCTLEFQDQRP
CCCCEEECCCEEEEEEECCCCCCCCCEEEEEHHHHHCHHHHHHHHHHHHHEEEEECCCCC
FYDWVIEETEFSIKPQQIEFSRLNLNYTITSKRKLKYLVDNKLVNGWDDPRMPTIKGYRR
HHHEEEECCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHH
RGYTPESIRNFCEMIGISKQDSVIDVSVLEDAVRDDLNKSVLRKNVVLDPIRVSIKDMPN
CCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCEECCCEEEEECCCC
HHLDVPNHPQDPEFGRREITISSQIFIERDDFVFKLEKDMKKLSPNGRVRLLNGYVIECQ
CCCCCCCCCCCCCCCCEEEEEEEEEEEECCCEEEEEHHHHHHCCCCCCEEEECCEEEEEH
EVITDTSGEVIELKCSYLPETLGGKKPNDGIKPNGIIHWVDANNCLDAEVRIYDRLFNDE
HHHCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCC
NPANADRIEDVLNPDSLQVIKNAKVERSLESVKAEERFQFNRVGYFIADLKDCSNDTLVF
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCCCCEEE
NHIATLRN
EHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]