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The map label for this gene is gmuE [H]

Identifier: 134302373

GI number: 134302373

Start: 1403403

End: 1404302

Strand: Direct

Name: gmuE [H]

Synonym: FTW_1497

Alternate gene names: 134302373

Gene position: 1403403-1404302 (Clockwise)

Preceding gene: 134302372

Following gene: 134302374

Centisome position: 73.92

GC content: 35.89

Gene sequence:

>900_bases
ATGTATTTAGCTGGTATAGAGGCTGGTGGAACAAAATTTTTTACAACGATTGGCGATTTTGATGGCAATGTGATAGAGCG
CCATCGCACAGATACAACAACCTCAGAAAAAACAATGTCTGAAGTTCTAAAGGTTCTAAAAGATTATCAAAACAAATATG
ATATTAAAACTATAGGCTTAGCATGCTTTGGACCTATTGACATTAATCCTAATTCGAAAACTTATGGGTATATTACAAAC
ACTCCAAAAATAGCATGGCAAAATTTTGATATTGTCAATGCTGTGAAGACAATTTTCTCAGGTCCAATCGGGTTTAATAC
AGATGTAAATGCCGCAGCAATCTGTGAAAAACTATGGGGCTGTGCTCAAGACTTAGAAAATCTACTTTATTTAACAGTTG
GCACTGGTGTTGGTGGTGGAATTATTTGTAATAACAAGCTAGTTCAGGGAGCGATGCATCCAGAAATTGGCCATTTATTA
ATACCACAAAATCCTCTAGATGAGTTTAAGGGCTCTTGTCCTTTCCACGGTAACTGTTTAGAAGGACTTGCTTCTGGCAC
AGCGATTAATCAAAGATGGAAAGTTGCTCATGCTAGCGCACTTAATGACGATCATATCGCGTGGCAATTTGAAGCCGAAT
ATTTAGCAAAAGCCATAGTGAACTATATATGTTCATTTTCGCCTGAGAGAATTATTCTCGGTGGCGGCGTAATGCATAAG
ACAATCTTATTTGACATGATTCGTAAAAATGTTACTAAATACCTAAATAACTATCTTGATTATCCTGCGTTAAAAGATAT
GACTAAATTTATAGTTCCTGCATCTTTTGGTGATAATACAGGTGTTAAAGGATCTCTTGCTCTTGCATTAGAAACTTTTA
ATAACTCTCAAGCATATTAA

Upstream 100 bases:

>100_bases
ATTTGTTATTTTTATTTCTTTCAAAGAGTAATAATCTAAATATTATTAGTGAATAATGCTAAAATCTTTATATCTAGTTT
TATTGAAAAAAGAGTGATTT

Downstream 100 bases:

>100_bases
TCTAGTAGAAATTATTCTTATATTATCTATACTTTACTGTTAAAATCTTTTTAGAGGTAATTCAATACTAAAACAAATTG
ATTAAAAAAATACTTTCTAA

Product: ROK family protein

Products: NA

Alternate protein names: Glucomannan utilization protein E [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MYLAGIEAGGTKFFTTIGDFDGNVIERHRTDTTTSEKTMSEVLKVLKDYQNKYDIKTIGLACFGPIDINPNSKTYGYITN
TPKIAWQNFDIVNAVKTIFSGPIGFNTDVNAAAICEKLWGCAQDLENLLYLTVGTGVGGGIICNNKLVQGAMHPEIGHLL
IPQNPLDEFKGSCPFHGNCLEGLASGTAINQRWKVAHASALNDDHIAWQFEAEYLAKAIVNYICSFSPERIILGGGVMHK
TILFDMIRKNVTKYLNNYLDYPALKDMTKFIVPASFGDNTGVKGSLALALETFNNSQAY

Sequences:

>Translated_299_residues
MYLAGIEAGGTKFFTTIGDFDGNVIERHRTDTTTSEKTMSEVLKVLKDYQNKYDIKTIGLACFGPIDINPNSKTYGYITN
TPKIAWQNFDIVNAVKTIFSGPIGFNTDVNAAAICEKLWGCAQDLENLLYLTVGTGVGGGIICNNKLVQGAMHPEIGHLL
IPQNPLDEFKGSCPFHGNCLEGLASGTAINQRWKVAHASALNDDHIAWQFEAEYLAKAIVNYICSFSPERIILGGGVMHK
TILFDMIRKNVTKYLNNYLDYPALKDMTKFIVPASFGDNTGVKGSLALALETFNNSQAY
>Mature_299_residues
MYLAGIEAGGTKFFTTIGDFDGNVIERHRTDTTTSEKTMSEVLKVLKDYQNKYDIKTIGLACFGPIDINPNSKTYGYITN
TPKIAWQNFDIVNAVKTIFSGPIGFNTDVNAAAICEKLWGCAQDLENLLYLTVGTGVGGGIICNNKLVQGAMHPEIGHLL
IPQNPLDEFKGSCPFHGNCLEGLASGTAINQRWKVAHASALNDDHIAWQFEAEYLAKAIVNYICSFSPERIILGGGVMHK
TILFDMIRKNVTKYLNNYLDYPALKDMTKFIVPASFGDNTGVKGSLALALETFNNSQAY

Specific function: Seems to be involved in the degradation of glucomannan [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI1790522, Length=308, Percent_Identity=25.974025974026, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786891, Length=263, Percent_Identity=26.2357414448669, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 32811; Mature: 32811

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLAGIEAGGTKFFTTIGDFDGNVIERHRTDTTTSEKTMSEVLKVLKDYQNKYDIKTIGL
CEEEEECCCCCEEEEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
ACFGPIDINPNSKTYGYITNTPKIAWQNFDIVNAVKTIFSGPIGFNTDVNAAAICEKLWG
EEEECEECCCCCCEEEEEECCCEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
CAQDLENLLYLTVGTGVGGGIICNNKLVQGAMHPEIGHLLIPQNPLDEFKGSCPFHGNCL
HHHHHHHEEEEEECCCCCCCEEECCHHHCCCCCCCCCEEECCCCCHHHHCCCCCCCCHHH
EGLASGTAINQRWKVAHASALNDDHIAWQFEAEYLAKAIVNYICSFSPERIILGGGVMHK
HHHHCCCCCCCHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCEEEEECCHHHH
TILFDMIRKNVTKYLNNYLDYPALKDMTKFIVPASFGDNTGVKGSLALALETFNNSQAY
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MYLAGIEAGGTKFFTTIGDFDGNVIERHRTDTTTSEKTMSEVLKVLKDYQNKYDIKTIGL
CEEEEECCCCCEEEEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
ACFGPIDINPNSKTYGYITNTPKIAWQNFDIVNAVKTIFSGPIGFNTDVNAAAICEKLWG
EEEECEECCCCCCEEEEEECCCEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
CAQDLENLLYLTVGTGVGGGIICNNKLVQGAMHPEIGHLLIPQNPLDEFKGSCPFHGNCL
HHHHHHHEEEEEECCCCCCCEEECCHHHCCCCCCCCCEEECCCCCHHHHCCCCCCCCHHH
EGLASGTAINQRWKVAHASALNDDHIAWQFEAEYLAKAIVNYICSFSPERIILGGGVMHK
HHHHCCCCCCCHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCEEEEECCHHHH
TILFDMIRKNVTKYLNNYLDYPALKDMTKFIVPASFGDNTGVKGSLALALETFNNSQAY
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]