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The map label for this gene is bcr [H]

Identifier: 134302345

GI number: 134302345

Start: 1365240

End: 1366454

Strand: Direct

Name: bcr [H]

Synonym: FTW_1463

Alternate gene names: 134302345

Gene position: 1365240-1366454 (Clockwise)

Preceding gene: 134302344

Following gene: 134302351

Centisome position: 71.91

GC content: 32.35

Gene sequence:

>1215_bases
ATGAAACTTATAACTAGAAATTCTAAATTATTCCCTATAATTTTGGCATTATTTGCTGCGTTACCACCTCTAGCTGTGAA
TACTTATGCTCCAGCGATTCCTTTAATAGCTAGTGATTTTGGTGTCAGTAATAGTACTGTTCTGACAACTTTTGCAACCT
ACTTTATTGGCTTCTCTTTTGGAATGCTATTTTGGGGAGCAGTATCTGATAAATATGGTCGCAAGAAAGTTATAATTATT
GGAAGTGTTATACATATTATAAGTACTATACTATGTTCTTATAGCTATAACTTCAGAATGCTTGAGATTATGCGTTTACT
TCAAGGCTTATCTGACTCTGTCGGTGCTGTAATAGCCTTTTCAATTGCTCGTGATTGCTACAAAGGTGCTAAACTTACCA
GTTTACTTGCATCTATAATCATAATATTACTAATTGCTCCTATCGTAGCGCCAATAATTGGTAGTATACTAACTGAACTA
ACTCACTCATGGCAAAGTACATTTCATTTTCTCACTATTTATGGTGTAGTCATGCTTATATGTGCATTCTTAATTGAAGA
AACTTTAGAACACAAAGATAGAAAATCAAGTCTACTAAAACTAATCCCTAGCTACTTCGAACATCTCACTAACCCTAGCT
TTATGTTAGCAACTATTGCTAGTGGCACTATTTTTGCAGCATTATTCATATATATATCATCATCAGCTTTGATATATCTC
GGTAATTATGCAACCAACTCTCTCTGGTATTGTTTATATTATGGTTTGACCTGTGTTAGCGCTATTTTTGCAAACATCAT
GATAAAGAAATACTCAAGCAAAGTACAACAGTTAAAATTCTTATATTACAGTGTCGGACTGATTACTCTAAGCTGCATTA
CTTTAATAATATTTAATACCTTAAATCTAGATAACGCTCTAATCTACACTCTAACAATGTTTGTATTATGTGGCAATGTT
GCTTTTAGCTCAACTCTTATCTATGCATTTGCTATCGATCAAATAGATCATAGTTTTGGTACTGCAAATTCAATTGTCAA
TTTTATAAAAAATATGATTGCAGCTGCTGGTAGCTATATCGTAAGCTTTTATCATGGTAGAGATTTAGTTATAGCCTCTC
CTTATACTCAACTAGTTTTTGTGTTGATTACTATAGTGATACTATTTGGAATACATAGATTAAACCAAAAAGCGAAACTA
CAACAATCTAACTAA

Upstream 100 bases:

>100_bases
CTACTAAATGGTTGGCGATATGAAATAGTTGGTAAAAAATTAAAAGAGTATATACTTAGTAACACGAAATAAAATTATTA
ATAACCATATTCTAATATCT

Downstream 100 bases:

>100_bases
GCATTGACATTAGAATAGGCTTTCTCAAATAGATATTCTTTTTTGATTACATCAAGAGCTGATTGATGAGTTGATTCAAG
TACAACTTCTGGCGCAACAC

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLITRNSKLFPIILALFAALPPLAVNTYAPAIPLIASDFGVSNSTVLTTFATYFIGFSFGMLFWGAVSDKYGRKKVIII
GSVIHIISTILCSYSYNFRMLEIMRLLQGLSDSVGAVIAFSIARDCYKGAKLTSLLASIIIILLIAPIVAPIIGSILTEL
THSWQSTFHFLTIYGVVMLICAFLIEETLEHKDRKSSLLKLIPSYFEHLTNPSFMLATIASGTIFAALFIYISSSALIYL
GNYATNSLWYCLYYGLTCVSAIFANIMIKKYSSKVQQLKFLYYSVGLITLSCITLIIFNTLNLDNALIYTLTMFVLCGNV
AFSSTLIYAFAIDQIDHSFGTANSIVNFIKNMIAAAGSYIVSFYHGRDLVIASPYTQLVFVLITIVILFGIHRLNQKAKL
QQSN

Sequences:

>Translated_404_residues
MKLITRNSKLFPIILALFAALPPLAVNTYAPAIPLIASDFGVSNSTVLTTFATYFIGFSFGMLFWGAVSDKYGRKKVIII
GSVIHIISTILCSYSYNFRMLEIMRLLQGLSDSVGAVIAFSIARDCYKGAKLTSLLASIIIILLIAPIVAPIIGSILTEL
THSWQSTFHFLTIYGVVMLICAFLIEETLEHKDRKSSLLKLIPSYFEHLTNPSFMLATIASGTIFAALFIYISSSALIYL
GNYATNSLWYCLYYGLTCVSAIFANIMIKKYSSKVQQLKFLYYSVGLITLSCITLIIFNTLNLDNALIYTLTMFVLCGNV
AFSSTLIYAFAIDQIDHSFGTANSIVNFIKNMIAAAGSYIVSFYHGRDLVIASPYTQLVFVLITIVILFGIHRLNQKAKL
QQSN
>Mature_404_residues
MKLITRNSKLFPIILALFAALPPLAVNTYAPAIPLIASDFGVSNSTVLTTFATYFIGFSFGMLFWGAVSDKYGRKKVIII
GSVIHIISTILCSYSYNFRMLEIMRLLQGLSDSVGAVIAFSIARDCYKGAKLTSLLASIIIILLIAPIVAPIIGSILTEL
THSWQSTFHFLTIYGVVMLICAFLIEETLEHKDRKSSLLKLIPSYFEHLTNPSFMLATIASGTIFAALFIYISSSALIYL
GNYATNSLWYCLYYGLTCVSAIFANIMIKKYSSKVQQLKFLYYSVGLITLSCITLIIFNTLNLDNALIYTLTMFVLCGNV
AFSSTLIYAFAIDQIDHSFGTANSIVNFIKNMIAAAGSYIVSFYHGRDLVIASPYTQLVFVLITIVILFGIHRLNQKAKL
QQSN

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=364, Percent_Identity=27.4725274725275, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1790146, Length=185, Percent_Identity=32.972972972973, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI48994889, Length=426, Percent_Identity=23.2394366197183, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787065, Length=275, Percent_Identity=22.9090909090909, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87082312, Length=193, Percent_Identity=26.9430051813472, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1790794, Length=309, Percent_Identity=24.2718446601942, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319657, Length=170, Percent_Identity=27.6470588235294, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44722; Mature: 44722

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLITRNSKLFPIILALFAALPPLAVNTYAPAIPLIASDFGVSNSTVLTTFATYFIGFSF
CCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHH
GMLFWGAVSDKYGRKKVIIIGSVIHIISTILCSYSYNFRMLEIMRLLQGLSDSVGAVIAF
HHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHH
SIARDCYKGAKLTSLLASIIIILLIAPIVAPIIGSILTELTHSWQSTFHFLTIYGVVMLI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAFLIEETLEHKDRKSSLLKLIPSYFEHLTNPSFMLATIASGTIFAALFIYISSSALIYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
GNYATNSLWYCLYYGLTCVSAIFANIMIKKYSSKVQQLKFLYYSVGLITLSCITLIIFNT
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNLDNALIYTLTMFVLCGNVAFSSTLIYAFAIDQIDHSFGTANSIVNFIKNMIAAAGSYI
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VSFYHGRDLVIASPYTQLVFVLITIVILFGIHRLNQKAKLQQSN
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCC
>Mature Secondary Structure
MKLITRNSKLFPIILALFAALPPLAVNTYAPAIPLIASDFGVSNSTVLTTFATYFIGFSF
CCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHH
GMLFWGAVSDKYGRKKVIIIGSVIHIISTILCSYSYNFRMLEIMRLLQGLSDSVGAVIAF
HHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHH
SIARDCYKGAKLTSLLASIIIILLIAPIVAPIIGSILTELTHSWQSTFHFLTIYGVVMLI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAFLIEETLEHKDRKSSLLKLIPSYFEHLTNPSFMLATIASGTIFAALFIYISSSALIYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
GNYATNSLWYCLYYGLTCVSAIFANIMIKKYSSKVQQLKFLYYSVGLITLSCITLIIFNT
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNLDNALIYTLTMFVLCGNVAFSSTLIYAFAIDQIDHSFGTANSIVNFIKNMIAAAGSYI
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VSFYHGRDLVIASPYTQLVFVLITIVILFGIHRLNQKAKLQQSN
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]