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The map label for this gene is purN [H]

Identifier: 134302214

GI number: 134302214

Start: 1216143

End: 1216718

Strand: Reverse

Name: purN [H]

Synonym: FTW_1284

Alternate gene names: 134302214

Gene position: 1216718-1216143 (Counterclockwise)

Preceding gene: 134302215

Following gene: 134302213

Centisome position: 64.09

GC content: 35.42

Gene sequence:

>576_bases
ATGTCTAAGCTAAATCTTGTTATATTAGGCTCTACGCGTGGGACTAATATGCAAGCTATAATAGATGCAATAGCAAATAA
GCAGCTAAATGCTCAGGTAAGCCTAGTAATTTCAAATAAGTCAGATGCTTATATATTACAAAGAGCAGCAGATTATAATA
TCCCAACTAAATATATAGCAGCTAAAGGTTTGACTAGAGAGCAGTATGATGAGCTAGTTGTTGCTGAAATCCAAAAATAT
AATCCAGATTTGATTTTGCTGATAGGCTTTATGCGTATTTTAAGCTCGGTGTTTATCAAAGCTTTTGAAGGTAAGATCTT
AAATATTCATCCATCACTATTGCCAAAGCACCGAGGATTAATGGATTTGGCAGTGCATCAGTCAGTTATCGACGCTGGAG
ATAGTATATCTGGATGCACTATCCATCAAGTAAGTGAAGAAGTCGATGGTGGTGATATAGTTTTGCAGTTAAAATGTGAT
GTGGTGAAAGAAGATACGGCTGATAGCTTAAAAGAAAAAGTACAAGCCTTAGAGAGTAAAGCTTGGATAGAAGTTATTAA
GAATTGGGATAAGTAA

Upstream 100 bases:

>100_bases
ATTTCATCGTCCAGATATTGGTACTAAAGAGCTTATCAATAAGCGTATCAAACACATGAATTTGTTGCGTGGTGATAAAT
ATCAGGAGCTTAAATAAATA

Downstream 100 bases:

>100_bases
TTTTTAATAAATGCCATACTACGATTTGAGCGTAGAATCTATTGATAGATATGGTGAATGGATCCTATAGTTTAAGCATA
GGATGACCGATTTGTGGGTT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MSKLNLVILGSTRGTNMQAIIDAIANKQLNAQVSLVISNKSDAYILQRAADYNIPTKYIAAKGLTREQYDELVVAEIQKY
NPDLILLIGFMRILSSVFIKAFEGKILNIHPSLLPKHRGLMDLAVHQSVIDAGDSISGCTIHQVSEEVDGGDIVLQLKCD
VVKEDTADSLKEKVQALESKAWIEVIKNWDK

Sequences:

>Translated_191_residues
MSKLNLVILGSTRGTNMQAIIDAIANKQLNAQVSLVISNKSDAYILQRAADYNIPTKYIAAKGLTREQYDELVVAEIQKY
NPDLILLIGFMRILSSVFIKAFEGKILNIHPSLLPKHRGLMDLAVHQSVIDAGDSISGCTIHQVSEEVDGGDIVLQLKCD
VVKEDTADSLKEKVQALESKAWIEVIKNWDK
>Mature_190_residues
SKLNLVILGSTRGTNMQAIIDAIANKQLNAQVSLVISNKSDAYILQRAADYNIPTKYIAAKGLTREQYDELVVAEIQKYN
PDLILLIGFMRILSSVFIKAFEGKILNIHPSLLPKHRGLMDLAVHQSVIDAGDSISGCTIHQVSEEVDGGDIVLQLKCDV
VKEDTADSLKEKVQALESKAWIEVIKNWDK

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=179, Percent_Identity=38.5474860335196, Blast_Score=130, Evalue=9e-31,
Organism=Homo sapiens, GI209869995, Length=179, Percent_Identity=38.5474860335196, Blast_Score=130, Evalue=9e-31,
Organism=Homo sapiens, GI209869993, Length=179, Percent_Identity=38.5474860335196, Blast_Score=130, Evalue=9e-31,
Organism=Escherichia coli, GI1788846, Length=187, Percent_Identity=41.7112299465241, Blast_Score=152, Evalue=1e-38,
Organism=Escherichia coli, GI1787483, Length=148, Percent_Identity=31.7567567567568, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789683, Length=142, Percent_Identity=30.9859154929577, Blast_Score=66, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17567511, Length=180, Percent_Identity=38.8888888888889, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320616, Length=205, Percent_Identity=32.6829268292683, Blast_Score=89, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24582400, Length=185, Percent_Identity=39.4594594594595, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21132; Mature: 21001

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLNLVILGSTRGTNMQAIIDAIANKQLNAQVSLVISNKSDAYILQRAADYNIPTKYIA
CCCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEHHCCCCCCHHHHH
AKGLTREQYDELVVAEIQKYNPDLILLIGFMRILSSVFIKAFEGKILNIHPSLLPKHRGL
HCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCH
MDLAVHQSVIDAGDSISGCTIHQVSEEVDGGDIVLQLKCDVVKEDTADSLKEKVQALESK
HHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
AWIEVIKNWDK
HHHHHHHCCCC
>Mature Secondary Structure 
SKLNLVILGSTRGTNMQAIIDAIANKQLNAQVSLVISNKSDAYILQRAADYNIPTKYIA
CCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEHHCCCCCCHHHHH
AKGLTREQYDELVVAEIQKYNPDLILLIGFMRILSSVFIKAFEGKILNIHPSLLPKHRGL
HCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCH
MDLAVHQSVIDAGDSISGCTIHQVSEEVDGGDIVLQLKCDVVKEDTADSLKEKVQALESK
HHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
AWIEVIKNWDK
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]