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The map label for this gene is proP [H]

Identifier: 134302170

GI number: 134302170

Start: 1165326

End: 1166597

Strand: Reverse

Name: proP [H]

Synonym: FTW_1237

Alternate gene names: 134302170

Gene position: 1166597-1165326 (Counterclockwise)

Preceding gene: 134302171

Following gene: 134302166

Centisome position: 61.45

GC content: 35.06

Gene sequence:

>1272_bases
GTGTACAAGGATATATCTCCTAAAAATGTAATCTTTGCTGGTTTGGCAACTAGTGTGGAGTATTTTGATTTTGCGTTATT
TGCCTATGTAACGGTTTTTATTTCAGCGGCTTTTTTTCCTGATAGTAACTCAACTGTGGCGACGATTAAAACATTTGGAA
TGTTTGCTGCTGGATATCTTATGCGTCCTATTGGTGGAATATTTTTTGGTAATCTAGGTGATAAAGTAGGGCGTAAAAAA
GTTTTGATTATAACGGTTGCTTTAATGGCGATTTCAACTGCGATTATCGCCTTTACTCCAAGCTATCAGCAAATCGGTAT
AGTTTCTGTACTTTTAATTATTTTTGCGCGGATGATACAAGGTTTTTCAATTGGTGGCGAGTATAATGGTGTGCTAGCAA
TCTTAAGTGAGCAAGCACCAGATAATAAGCGTGGATTAATTACTGCATTTGGAACATTCTTTTCTGGATTTGGGGTATTT
TTGGGTACGGTAGTTGTATTTTTGTTCTCTAGTTTTTTGTCAGAATCACAAATGCACTTATATGGATGGAGACTATGCTT
TGTAATAGGTATGATAATGGCTATATTAGTGCTTATATTACAATTTAGACAAGATGAGTCACCAGAGTATCTAAGAGCTA
AACACGATAATCTTCTTGAAGATACTCCTGTGGTTTCAGCTATTAGAGAATATCCTTATCAAATATTTGTGGTGTTTGCC
CTAGCTGGTTATTTAGGCATTGTTTATTATATGGTTTCGGCATTTATTCCATCTTTATTAGAAGGTTCGTTACATTACTC
GACAAATGTGGCAATGCTAGTAACTATGGTCGCATCAATATGCTATTTTGCTACAACTCCATTATGGGGTATGATTTCAG
ACAAGGTAGGGCGTAAGAAAGTCTTACTAACAGCTTGTATAGGAGTTGGAATATTAATTTATCCTAGTTTATTTATTATT
AATAGTATGAATTCACCAATATTATCTTGCATAGTAATGTCATTATTAATGCTACTTATATCAGCAGCGACAGCAACATT
TGTAGTGGCTATTAATGAGCTTTTCCCAACGAGTCTTAGATTTAGCGGTGTTGCAACTGGTTATAATGTTAGTAATGCAC
TATTAGGCGGAACAGTGCCATTAGTTTCAAGTATCTTGATTGTATATTTTGGTCAGATGTCACCAGGTATTTATGCAATT
ATAGCGTCGGTAGTAATTGTAGTGATTATCGCAAAAATGCCTGAAACTAGAGGGATTGAATTGGAAGAATAA

Upstream 100 bases:

>100_bases
TAGATTAATCATTGCTTATTTATTATTAATTACACCGGTTTTTTGCAAATTAAATTTTAATTCTATAATATATCGAAAAC
TAAACTAAATTATATCAAAA

Downstream 100 bases:

>100_bases
TAAAGAGTTAATAGCATCTTTTTTTAAGATAATCACACAATAATGAAATCATAATACCAGCAAAAATCCATTGCATTGTG
AATTGAGAAATACTTAATAG

Product: major facilitator superfamily transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MYKDISPKNVIFAGLATSVEYFDFALFAYVTVFISAAFFPDSNSTVATIKTFGMFAAGYLMRPIGGIFFGNLGDKVGRKK
VLIITVALMAISTAIIAFTPSYQQIGIVSVLLIIFARMIQGFSIGGEYNGVLAILSEQAPDNKRGLITAFGTFFSGFGVF
LGTVVVFLFSSFLSESQMHLYGWRLCFVIGMIMAILVLILQFRQDESPEYLRAKHDNLLEDTPVVSAIREYPYQIFVVFA
LAGYLGIVYYMVSAFIPSLLEGSLHYSTNVAMLVTMVASICYFATTPLWGMISDKVGRKKVLLTACIGVGILIYPSLFII
NSMNSPILSCIVMSLLMLLISAATATFVVAINELFPTSLRFSGVATGYNVSNALLGGTVPLVSSILIVYFGQMSPGIYAI
IASVVIVVIIAKMPETRGIELEE

Sequences:

>Translated_423_residues
MYKDISPKNVIFAGLATSVEYFDFALFAYVTVFISAAFFPDSNSTVATIKTFGMFAAGYLMRPIGGIFFGNLGDKVGRKK
VLIITVALMAISTAIIAFTPSYQQIGIVSVLLIIFARMIQGFSIGGEYNGVLAILSEQAPDNKRGLITAFGTFFSGFGVF
LGTVVVFLFSSFLSESQMHLYGWRLCFVIGMIMAILVLILQFRQDESPEYLRAKHDNLLEDTPVVSAIREYPYQIFVVFA
LAGYLGIVYYMVSAFIPSLLEGSLHYSTNVAMLVTMVASICYFATTPLWGMISDKVGRKKVLLTACIGVGILIYPSLFII
NSMNSPILSCIVMSLLMLLISAATATFVVAINELFPTSLRFSGVATGYNVSNALLGGTVPLVSSILIVYFGQMSPGIYAI
IASVVIVVIIAKMPETRGIELEE
>Mature_423_residues
MYKDISPKNVIFAGLATSVEYFDFALFAYVTVFISAAFFPDSNSTVATIKTFGMFAAGYLMRPIGGIFFGNLGDKVGRKK
VLIITVALMAISTAIIAFTPSYQQIGIVSVLLIIFARMIQGFSIGGEYNGVLAILSEQAPDNKRGLITAFGTFFSGFGVF
LGTVVVFLFSSFLSESQMHLYGWRLCFVIGMIMAILVLILQFRQDESPEYLRAKHDNLLEDTPVVSAIREYPYQIFVVFA
LAGYLGIVYYMVSAFIPSLLEGSLHYSTNVAMLVTMVASICYFATTPLWGMISDKVGRKKVLLTACIGVGILIYPSLFII
NSMNSPILSCIVMSLLMLLISAATATFVVAINELFPTSLRFSGVATGYNVSNALLGGTVPLVSSILIVYFGQMSPGIYAI
IASVVIVVIIAKMPETRGIELEE

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=427, Percent_Identity=32.3185011709602, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1788292, Length=388, Percent_Identity=29.639175257732, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1788942, Length=367, Percent_Identity=29.4277929155313, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=28.9044289044289, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI87082404, Length=396, Percent_Identity=25.7575757575758, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790463, Length=443, Percent_Identity=25.2821670428894, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082231, Length=203, Percent_Identity=26.6009852216749, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46012; Mature: 46012

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKDISPKNVIFAGLATSVEYFDFALFAYVTVFISAAFFPDSNSTVATIKTFGMFAAGYL
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHH
MRPIGGIFFGNLGDKVGRKKVLIITVALMAISTAIIAFTPSYQQIGIVSVLLIIFARMIQ
HHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHC
GFSIGGEYNGVLAILSEQAPDNKRGLITAFGTFFSGFGVFLGTVVVFLFSSFLSESQMHL
CCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
YGWRLCFVIGMIMAILVLILQFRQDESPEYLRAKHDNLLEDTPVVSAIREYPYQIFVVFA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHH
LAGYLGIVYYMVSAFIPSLLEGSLHYSTNVAMLVTMVASICYFATTPLWGMISDKVGRKK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHH
VLLTACIGVGILIYPSLFIINSMNSPILSCIVMSLLMLLISAATATFVVAINELFPTSLR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
FSGVATGYNVSNALLGGTVPLVSSILIVYFGQMSPGIYAIIASVVIVVIIAKMPETRGIE
ECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
LEE
CCC
>Mature Secondary Structure
MYKDISPKNVIFAGLATSVEYFDFALFAYVTVFISAAFFPDSNSTVATIKTFGMFAAGYL
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHH
MRPIGGIFFGNLGDKVGRKKVLIITVALMAISTAIIAFTPSYQQIGIVSVLLIIFARMIQ
HHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHC
GFSIGGEYNGVLAILSEQAPDNKRGLITAFGTFFSGFGVFLGTVVVFLFSSFLSESQMHL
CCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
YGWRLCFVIGMIMAILVLILQFRQDESPEYLRAKHDNLLEDTPVVSAIREYPYQIFVVFA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHH
LAGYLGIVYYMVSAFIPSLLEGSLHYSTNVAMLVTMVASICYFATTPLWGMISDKVGRKK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHH
VLLTACIGVGILIYPSLFIINSMNSPILSCIVMSLLMLLISAATATFVVAINELFPTSLR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
FSGVATGYNVSNALLGGTVPLVSSILIVYFGQMSPGIYAIIASVVIVVIIAKMPETRGIE
ECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
LEE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]