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The map label for this gene is pilB [H]

Identifier: 134302124

GI number: 134302124

Start: 1106169

End: 1107947

Strand: Direct

Name: pilB [H]

Synonym: FTW_1166

Alternate gene names: 134302124

Gene position: 1106169-1107947 (Clockwise)

Preceding gene: 134302122

Following gene: 134302125

Centisome position: 58.27

GC content: 34.23

Gene sequence:

>1779_bases
ATGATTGAAAATCCACATATCTGCAAAAAACTTGCTTCGCTATTACTTCATCGTAAGCTTATCACAAAGCAACAGCTTGA
AGAAATAAGTCACGATAAGTCTTTAGCAAAACAAGACTTTTTAGAATACTTGATTGAAAATGAGATCGTTGATAGTAAAT
CTTTTATGATAGAGTCTGCAACATTACTACGTTTGCAATATATTGACTTAACTACCATAAATGTAAAATTTTTACCTCAA
GAATACTTTGACATTGATTTTTGTCGCGAAAATCACTGTCTAGCACTATTTACACGCAAAAGGACTATTCATGTAGCAAT
AGCTAACCCTATTGAAAGTCAACAAATTCTTAAAAAACTTCGCAGTAGATATGATTGTGCATTCATCCCTGTAGTTGCTG
AACTAAATCATCTTAAAGAAAAAATAGAAGAATATGAACAATATCTCAAAGATGAGGGGCAAAGAGAGGATGACGCAAAG
CTTAATGAAAGAATTGATGCAGGATCAGAGCTTGATATTGACTTTGTTGAAGAAGGTGAGCGTGAAGGCGATGCAATAAT
AGGTAGTGGCGAAGATGAAGAAGCTCCTATTATTCGCTTTATCAATAATACTATAGTAGATGCCATCCAAAAAGGTGCTT
CGGATATACATTTTGAACCATATGAAAAAAACTTTCGCATCCGCTATAGGATAGACGGTCTTCTAATTGAGACTTTTAAT
ACTAAGAAAAATTTAGCTCCTAAGGTTATTTCTAGACTTAAAATTATGTCAAGCTTAGATATTGCTGAAAAGAGGATTCC
TCAGGATGGTAAATTTAAAATTTCTCTTTCACGTGAAAAAGCCATCGACTTTCGTGTAAGTACATGTCCTATTAGTTTCG
GTGAAAAAGTTGTACTGCGTATTATTGACTCAAGCTCGACACAAATACCAATAGAACAATTAGGTTTTTCAGAATCACAA
AAAGAAACCTATCTTAAATATATTCAACAACCTCAAGGCATGGTACTAGTAACAGGACCAACAGGTTCTGGTAAAACTGT
TACTTTATATACAGGTATTAATATTCTAAATAAGCCCGAAAAAAATATTTCAACCGCCGAAGACCCTGTTGAGCTAATTG
TAAAAGGTATTAACCAAGTAAGTGTTAATAATAAACAAGGACTTACTTTCGCAGCAGCACTTAAGTCTTTCTTACGTCAA
GATCCAGATATAATCATGGTCGGGGAGATTAGAGATATCGAAACAGGTTCAATTGCAATCAAAGCTTCTCAAACAGGTCA
CTTAGTTATGTCAACATTACATACAAACAGTGCTCCAGAGACATTAAACAGACTTGTGGATATGGGCTTGCCTCGATACA
ATATTGCTACATCTGTAACATTAATTATCGCTCAACGTCTAACTCGAAAGCTATGTCCTAAGTGTAAATTACCAGATACT
GATACAGAATTTTCACTTCTTGTCGAAGATAGTGGTTTAAATGATGAGATATTAGCAAAAACATTTGGAACAACTCTAGA
TAAAGTAAAAAATGCTAAAATTTACAAAGCTAATCCTAAAGGTTGCCCTAGATGTTTTAAGGGTTACAAAGGCAGAATTG
GTTTATATGAAGTTATGCCAGTATCAAGGCAAATATCAAGAATGATATTAGAAGATAAAAACACTATGGAAATTGCGATA
CAAGCTCAAAAAGAAGGAATCGCTACTGTCAGACAATCAGCTCTAGTCAGGGTTGCTGAAGATCTAACATCAATGGAAGA
AGCATACCGCGTAAGTTAA

Upstream 100 bases:

>100_bases
CTAAGCTATTTTTGATTAATTTATCTTAAATATTTTGTTTTTTAATAATTATCTTAATCATGCTATAATTTTGCTACTAT
TTGAGATTTATTTTATATAG

Downstream 100 bases:

>100_bases
AGGGAATAAAAAATATGCTATTTGGCAAAAAAGATAAAAATAAAAGAACTATTACATCATGGAATTATAAAGCTAAGCTA
AAAAGTGGTAAAAAGACTAA

Product: type IV-A pilus assembly ATPase/type II secretion system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MIENPHICKKLASLLLHRKLITKQQLEEISHDKSLAKQDFLEYLIENEIVDSKSFMIESATLLRLQYIDLTTINVKFLPQ
EYFDIDFCRENHCLALFTRKRTIHVAIANPIESQQILKKLRSRYDCAFIPVVAELNHLKEKIEEYEQYLKDEGQREDDAK
LNERIDAGSELDIDFVEEGEREGDAIIGSGEDEEAPIIRFINNTIVDAIQKGASDIHFEPYEKNFRIRYRIDGLLIETFN
TKKNLAPKVISRLKIMSSLDIAEKRIPQDGKFKISLSREKAIDFRVSTCPISFGEKVVLRIIDSSSTQIPIEQLGFSESQ
KETYLKYIQQPQGMVLVTGPTGSGKTVTLYTGINILNKPEKNISTAEDPVELIVKGINQVSVNNKQGLTFAAALKSFLRQ
DPDIIMVGEIRDIETGSIAIKASQTGHLVMSTLHTNSAPETLNRLVDMGLPRYNIATSVTLIIAQRLTRKLCPKCKLPDT
DTEFSLLVEDSGLNDEILAKTFGTTLDKVKNAKIYKANPKGCPRCFKGYKGRIGLYEVMPVSRQISRMILEDKNTMEIAI
QAQKEGIATVRQSALVRVAEDLTSMEEAYRVS

Sequences:

>Translated_592_residues
MIENPHICKKLASLLLHRKLITKQQLEEISHDKSLAKQDFLEYLIENEIVDSKSFMIESATLLRLQYIDLTTINVKFLPQ
EYFDIDFCRENHCLALFTRKRTIHVAIANPIESQQILKKLRSRYDCAFIPVVAELNHLKEKIEEYEQYLKDEGQREDDAK
LNERIDAGSELDIDFVEEGEREGDAIIGSGEDEEAPIIRFINNTIVDAIQKGASDIHFEPYEKNFRIRYRIDGLLIETFN
TKKNLAPKVISRLKIMSSLDIAEKRIPQDGKFKISLSREKAIDFRVSTCPISFGEKVVLRIIDSSSTQIPIEQLGFSESQ
KETYLKYIQQPQGMVLVTGPTGSGKTVTLYTGINILNKPEKNISTAEDPVELIVKGINQVSVNNKQGLTFAAALKSFLRQ
DPDIIMVGEIRDIETGSIAIKASQTGHLVMSTLHTNSAPETLNRLVDMGLPRYNIATSVTLIIAQRLTRKLCPKCKLPDT
DTEFSLLVEDSGLNDEILAKTFGTTLDKVKNAKIYKANPKGCPRCFKGYKGRIGLYEVMPVSRQISRMILEDKNTMEIAI
QAQKEGIATVRQSALVRVAEDLTSMEEAYRVS
>Mature_592_residues
MIENPHICKKLASLLLHRKLITKQQLEEISHDKSLAKQDFLEYLIENEIVDSKSFMIESATLLRLQYIDLTTINVKFLPQ
EYFDIDFCRENHCLALFTRKRTIHVAIANPIESQQILKKLRSRYDCAFIPVVAELNHLKEKIEEYEQYLKDEGQREDDAK
LNERIDAGSELDIDFVEEGEREGDAIIGSGEDEEAPIIRFINNTIVDAIQKGASDIHFEPYEKNFRIRYRIDGLLIETFN
TKKNLAPKVISRLKIMSSLDIAEKRIPQDGKFKISLSREKAIDFRVSTCPISFGEKVVLRIIDSSSTQIPIEQLGFSESQ
KETYLKYIQQPQGMVLVTGPTGSGKTVTLYTGINILNKPEKNISTAEDPVELIVKGINQVSVNNKQGLTFAAALKSFLRQ
DPDIIMVGEIRDIETGSIAIKASQTGHLVMSTLHTNSAPETLNRLVDMGLPRYNIATSVTLIIAQRLTRKLCPKCKLPDT
DTEFSLLVEDSGLNDEILAKTFGTTLDKVKNAKIYKANPKGCPRCFKGYKGRIGLYEVMPVSRQISRMILEDKNTMEIAI
QAQKEGIATVRQSALVRVAEDLTSMEEAYRVS

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=407, Percent_Identity=43.7346437346437, Blast_Score=338, Evalue=6e-94,
Organism=Escherichia coli, GI1786296, Length=401, Percent_Identity=41.3965087281795, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI87082188, Length=273, Percent_Identity=32.6007326007326, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 66925; Mature: 66925

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00124 FBPASE ; PS00211 ABC_TRANSPORTER_1 ; PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIENPHICKKLASLLLHRKLITKQQLEEISHDKSLAKQDFLEYLIENEIVDSKSFMIESA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHE
TLLRLQYIDLTTINVKFLPQEYFDIDFCRENHCLALFTRKRTIHVAIANPIESQQILKKL
EEEEEEEEEEEEEEEEECCHHHCCCEEECCCCEEEEEEECEEEEEEECCCCCHHHHHHHH
RSRYDCAFIPVVAELNHLKEKIEEYEQYLKDEGQREDDAKLNERIDAGSELDIDFVEEGE
HHHCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHCCC
REGDAIIGSGEDEEAPIIRFINNTIVDAIQKGASDIHFEPYEKNFRIRYRIDGLLIETFN
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEEEEC
TKKNLAPKVISRLKIMSSLDIAEKRIPQDGKFKISLSREKAIDFRVSTCPISFGEKVVLR
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEE
IIDSSSTQIPIEQLGFSESQKETYLKYIQQPQGMVLVTGPTGSGKTVTLYTGINILNKPE
EECCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCCCCC
KNISTAEDPVELIVKGINQVSVNNKQGLTFAAALKSFLRQDPDIIMVGEIRDIETGSIAI
CCCCCCCHHHHHHHHCCHHEECCCCCCCCHHHHHHHHHHCCCCEEEEECCEECCCCCEEE
KASQTGHLVMSTLHTNSAPETLNRLVDMGLPRYNIATSVTLIIAQRLTRKLCPKCKLPDT
EECCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
DTEFSLLVEDSGLNDEILAKTFGTTLDKVKNAKIYKANPKGCPRCFKGYKGRIGLYEVMP
CCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHCCCCCCCEEEECC
VSRQISRMILEDKNTMEIAIQAQKEGIATVRQSALVRVAEDLTSMEEAYRVS
HHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIENPHICKKLASLLLHRKLITKQQLEEISHDKSLAKQDFLEYLIENEIVDSKSFMIESA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHE
TLLRLQYIDLTTINVKFLPQEYFDIDFCRENHCLALFTRKRTIHVAIANPIESQQILKKL
EEEEEEEEEEEEEEEEECCHHHCCCEEECCCCEEEEEEECEEEEEEECCCCCHHHHHHHH
RSRYDCAFIPVVAELNHLKEKIEEYEQYLKDEGQREDDAKLNERIDAGSELDIDFVEEGE
HHHCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHCCC
REGDAIIGSGEDEEAPIIRFINNTIVDAIQKGASDIHFEPYEKNFRIRYRIDGLLIETFN
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEEEEC
TKKNLAPKVISRLKIMSSLDIAEKRIPQDGKFKISLSREKAIDFRVSTCPISFGEKVVLR
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEEE
IIDSSSTQIPIEQLGFSESQKETYLKYIQQPQGMVLVTGPTGSGKTVTLYTGINILNKPE
EECCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCCCCC
KNISTAEDPVELIVKGINQVSVNNKQGLTFAAALKSFLRQDPDIIMVGEIRDIETGSIAI
CCCCCCCHHHHHHHHCCHHEECCCCCCCCHHHHHHHHHHCCCCEEEEECCEECCCCCEEE
KASQTGHLVMSTLHTNSAPETLNRLVDMGLPRYNIATSVTLIIAQRLTRKLCPKCKLPDT
EECCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
DTEFSLLVEDSGLNDEILAKTFGTTLDKVKNAKIYKANPKGCPRCFKGYKGRIGLYEVMP
CCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHCCCCCCCEEEECC
VSRQISRMILEDKNTMEIAIQAQKEGIATVRQSALVRVAEDLTSMEEAYRVS
HHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]