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The map label for this gene is nucA [H]

Identifier: 134302082

GI number: 134302082

Start: 1059877

End: 1060941

Strand: Reverse

Name: nucA [H]

Synonym: FTW_1118

Alternate gene names: 134302082

Gene position: 1060941-1059877 (Counterclockwise)

Preceding gene: 134302083

Following gene: 134302080

Centisome position: 55.88

GC content: 32.77

Gene sequence:

>1065_bases
ATGACAAAAAAAACATCTAAGCAAAAAGATACTAAAAAACAAACCACTAAGAAGCCGATAAATTATATAAAAATATTTAT
ACTACTAGCTTTAACTATTGGTGGCGGATTTTCCGGTATTTTTTTTGACAAGTTTGATTTAAAAGAAAAATTTATAAACT
TTAGGCATGCTGTATATAACTATTTCACTAGTGAACCAATAAAGCACTATACCACCAAAGATAACGAGAATATTATTGCT
AGATTTTTTTCACAAAGTGATGAGGGGAAAAAACAACAGCAACAATCAAATGGGTTTGACAACCTCAAGCAGTATCCACC
AGTTAGAACTCTACCAGCAGTTACTGATTATTGCCATGGTTTTCTAGCTTATGGTAATCCTAGTTATGGTGTAACTGATG
GTTTAGGTCAAGCAAATCTTTATCTATGTCGTGATGGTTATGTTGTTGGTTATAACTATCAAACTAAAGAGGCAAGCTGG
GTAGCATTTAAGTTGACAAAGTCAAAAGTAGCAAATCATCTTAAACGTGATGATAGATTTAAAGAAGACAGTGATGTGCC
ATTTGTCTATAGAGCGACATTAGATGATTATTCTCGTTCTGGTTATGACAGAGGACATCTAGCTTCATATGCATCAATGG
ACTTTAGCAAGAAATCTGCAGATCAATCATTCTTATTATCAAATATGTCACCACAAAAAGCAGGGCTTAATAGGCAAGGC
TGGGAGAGGCTAGAAACGTATGAGCGAATCTGGGCAAATATGTATGATAGTATATATGTCTACACAGGACCAATATATAA
AAAACAAAAAATTCATAAAACTATAGGTGATAATAAGATAGCTGTACCTGATTATTTTTTTAAGATAATTTATGTACCTT
CTAAAAATCAAGCAATAGCATTTGTAATGCCTAATGCAAGAGTTGAAAAGACTAAGATTGCTAACTATAGGGTTTCTATA
AAAGATATTGAGCAATGTACCGGATTGCATTTTTTGACTAATATTTCAAATAGAGATGCTGTGATAAATAGCGTCTCCTC
AATGTGGCGTACCGCTTATTTATAA

Upstream 100 bases:

>100_bases
TAGTATTATCTTTATTATAAAAACAGTAAGAAATTCTTATTTTAAGAAAAAAGTTAATAGTTTTTAACTTTTTACAAAAT
AAACTAAAAAGTATTTAAGC

Downstream 100 bases:

>100_bases
GTGTTAGTCAGATCATTAGCTATTAATTTAGCTGCTTGTTGAATAATTTCTTCATTGCTTGGTGCATTTTTATCATTAGG
GTTAGTATATAAAATTCCTA

Product: DNA/RNA endonuclease

Products: NA

Alternate protein names: Endonuclease [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MTKKTSKQKDTKKQTTKKPINYIKIFILLALTIGGGFSGIFFDKFDLKEKFINFRHAVYNYFTSEPIKHYTTKDNENIIA
RFFSQSDEGKKQQQQSNGFDNLKQYPPVRTLPAVTDYCHGFLAYGNPSYGVTDGLGQANLYLCRDGYVVGYNYQTKEASW
VAFKLTKSKVANHLKRDDRFKEDSDVPFVYRATLDDYSRSGYDRGHLASYASMDFSKKSADQSFLLSNMSPQKAGLNRQG
WERLETYERIWANMYDSIYVYTGPIYKKQKIHKTIGDNKIAVPDYFFKIIYVPSKNQAIAFVMPNARVEKTKIANYRVSI
KDIEQCTGLHFLTNISNRDAVINSVSSMWRTAYL

Sequences:

>Translated_354_residues
MTKKTSKQKDTKKQTTKKPINYIKIFILLALTIGGGFSGIFFDKFDLKEKFINFRHAVYNYFTSEPIKHYTTKDNENIIA
RFFSQSDEGKKQQQQSNGFDNLKQYPPVRTLPAVTDYCHGFLAYGNPSYGVTDGLGQANLYLCRDGYVVGYNYQTKEASW
VAFKLTKSKVANHLKRDDRFKEDSDVPFVYRATLDDYSRSGYDRGHLASYASMDFSKKSADQSFLLSNMSPQKAGLNRQG
WERLETYERIWANMYDSIYVYTGPIYKKQKIHKTIGDNKIAVPDYFFKIIYVPSKNQAIAFVMPNARVEKTKIANYRVSI
KDIEQCTGLHFLTNISNRDAVINSVSSMWRTAYL
>Mature_353_residues
TKKTSKQKDTKKQTTKKPINYIKIFILLALTIGGGFSGIFFDKFDLKEKFINFRHAVYNYFTSEPIKHYTTKDNENIIAR
FFSQSDEGKKQQQQSNGFDNLKQYPPVRTLPAVTDYCHGFLAYGNPSYGVTDGLGQANLYLCRDGYVVGYNYQTKEASWV
AFKLTKSKVANHLKRDDRFKEDSDVPFVYRATLDDYSRSGYDRGHLASYASMDFSKKSADQSFLLSNMSPQKAGLNRQGW
ERLETYERIWANMYDSIYVYTGPIYKKQKIHKTIGDNKIAVPDYFFKIIYVPSKNQAIAFVMPNARVEKTKIANYRVSIK
DIEQCTGLHFLTNISNRDAVINSVSSMWRTAYL

Specific function: Catalyzes the degradation of both RNA and DNA; has the potential to act as an endonuclease [H]

COG id: COG1864

COG function: function code F; DNA/RNA endonuclease G, NUC1

Gene ontology:

Cell location: Periplasm. Note=Periplasmic or loosely attached to the cytoplasmic or the outer membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family [H]

Homologues:

Organism=Homo sapiens, GI53759134, Length=232, Percent_Identity=35.7758620689655, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI224451073, Length=222, Percent_Identity=29.2792792792793, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI224451075, Length=185, Percent_Identity=30.8108108108108, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17505967, Length=214, Percent_Identity=35.0467289719626, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6322253, Length=252, Percent_Identity=36.1111111111111, Blast_Score=132, Evalue=8e-32,
Organism=Drosophila melanogaster, GI20129899, Length=208, Percent_Identity=34.1346153846154, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24581065, Length=228, Percent_Identity=32.4561403508772, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28571642, Length=229, Percent_Identity=28.3842794759825, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018524
- InterPro:   IPR001604
- InterPro:   IPR020821 [H]

Pfam domain/function: PF01223 Endonuclease_NS [H]

EC number: 3.1.30.-

Molecular weight: Translated: 40852; Mature: 40721

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS01070 NUCLEASE_NON_SPEC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKTSKQKDTKKQTTKKPINYIKIFILLALTIGGGFSGIFFDKFDLKEKFINFRHAVYN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHH
YFTSEPIKHYTTKDNENIIARFFSQSDEGKKQQQQSNGFDNLKQYPPVRTLPAVTDYCHG
HHHCCCHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHCC
FLAYGNPSYGVTDGLGQANLYLCRDGYVVGYNYQTKEASWVAFKLTKSKVANHLKRDDRF
EEEECCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHCCC
KEDSDVPFVYRATLDDYSRSGYDRGHLASYASMDFSKKSADQSFLLSNMSPQKAGLNRQG
CCCCCCCEEEEECHHHHHHCCCCCCHHHHHHHCCCCHHCCCHHHHHCCCCHHHCCCCHHH
WERLETYERIWANMYDSIYVYTGPIYKKQKIHKTIGDNKIAVPDYFFKIIYVPSKNQAIA
HHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCEECCCEEEEEEEECCCCCEEE
FVMPNARVEKTKIANYRVSIKDIEQCTGLHFLTNISNRDAVINSVSSMWRTAYL
EEECCCCCCCEEECEEEEEHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKKTSKQKDTKKQTTKKPINYIKIFILLALTIGGGFSGIFFDKFDLKEKFINFRHAVYN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHH
YFTSEPIKHYTTKDNENIIARFFSQSDEGKKQQQQSNGFDNLKQYPPVRTLPAVTDYCHG
HHHCCCHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHCC
FLAYGNPSYGVTDGLGQANLYLCRDGYVVGYNYQTKEASWVAFKLTKSKVANHLKRDDRF
EEEECCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHCCC
KEDSDVPFVYRATLDDYSRSGYDRGHLASYASMDFSKKSADQSFLLSNMSPQKAGLNRQG
CCCCCCCEEEEECHHHHHHCCCCCCHHHHHHHCCCCHHCCCHHHHHCCCCHHHCCCCHHH
WERLETYERIWANMYDSIYVYTGPIYKKQKIHKTIGDNKIAVPDYFFKIIYVPSKNQAIA
HHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCEECCCEEEEEEEECCCCCEEE
FVMPNARVEKTKIANYRVSIKDIEQCTGLHFLTNISNRDAVINSVSSMWRTAYL
EEECCCCCCCEEECEEEEEHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1343821; 11759840 [H]