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The map label for this gene is lcfB [H]

Identifier: 134301855

GI number: 134301855

Start: 815906

End: 817276

Strand: Direct

Name: lcfB [H]

Synonym: FTW_0838

Alternate gene names: 134301855

Gene position: 815906-817276 (Clockwise)

Preceding gene: 134301849

Following gene: 134301856

Centisome position: 42.98

GC content: 28.88

Gene sequence:

>1371_bases
ATGATTCAGCTCGATAAATTTTTGGCAAATATTAAAAATTATGGCAAAAATAACTATATCTTCTTTGAAGATAAAAGATA
TGACTATGTTGAAATTCTACAAATATCATATAGTCTAGCGAGATTTTTGGAATCTTATGGCTATAGGAAAGTATATTCAA
ATCTTAAAAATTCACCATTATTAGTGAGTTTGTATATAGCTAGTTGGATAGCTGATATAGATCTTTTTGTACCAATCAAC
CCAAGATTAGTCGATAACGAGCTAGCAGCAATAGTAGATGGTGATAGTTTGTTTATCACAGATAAAATATCTAATCTTGA
AGTAAATAAATTATATATAGAAAATGCAACATGTTTTTTTGAGAGCCTTGAATATACACAAGATTATATAACTCATGCTA
AAGCTCTTACAGCACATGTAAGCTCAGGGACAACAGGATTTTATCAAAAATATCTTCATAAAATTGATCAGATAATTAAA
TATGCTAATGATAGAATCGCTGATTTAGGTCTCAAACAAGATGATCATTTGCTAGTAGCATTATCGATAAATCATGCTTT
TGCTTTTTCTTATCAAATTTTACCAGCATTAGCTATAGGATTAGATATAACAATAATCCGCGAGTTCGATGCTAAGTTAG
TAGCACGGATTATTAACCAAAATAGTGTAACCGCTTTAGCATTGTTACCGATAATGTATCATTTTTTGTTAGAGCAAAAT
ATTAACAAAAATCATAACCTACGCTACCTAAGTGTTGCTGGTGATGTCGCTAGTATAAGCTTAGTAACAGCTGTAAAAGA
TAAATTAGGCTTACCATTACTAAATGGTATAGGTATGACAGAAGTCTTTGGTTATGGTCAAAATATATCTGCTAATACAG
TTAATAAAGTTAAAATATTCTCAGATACTGAAGTAAAAATACTTAAATTTGAAAATAGTAACTATGGTAAAATCTTCATC
AAAAATAATATGCTACCTCTAAATATCGAAACTGAGTGGTTAGAAACTGGAGATATTGGTAGTTTTGATCACCAAAGCTA
TGAGCTTAGTTTTTATGGTCGCTATAAGGATATAATTATTAAAGGCGGCTCTAATATATCTCCGTTTGAATTAGAAACAG
CAATTTTAAAAATACCAAATATTGATAATTGCATAGTTACTTCTAAAAAGGATAAAATATGGGGAGAAACTGTTTGGGCA
TATTTGGTAGCATCGCAACAATACTCATTAGAGTTTATAAATAACCAGCTAAGAAATTATATAGCTGAATACAAAAAATT
AGATGGAATTATTTATATTGATGAAATCCCAATAACTACAACAGGTAAAACTAATAGAAAAAAAATAAAAGAAATGATTA
ATCATGAGTAA

Upstream 100 bases:

>100_bases
AAAATAAAGATTGTTTCATTTGTTAGGTAAAATTACAGGTAAATTTTTATATTTAAGATATTATATCAGCAAATACTAAG
ATAATTAATTGCTACAAAAA

Downstream 100 bases:

>100_bases
TAACCAATTACCACAATTTGCAATTCTTGAAGATACTCTAACTGATAAACAAAAATATTATTTTTATAATCCGATTGCAG
AGGTTATAGCTAATAGTAAG

Product: AMP-binding enzyme

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MIQLDKFLANIKNYGKNNYIFFEDKRYDYVEILQISYSLARFLESYGYRKVYSNLKNSPLLVSLYIASWIADIDLFVPIN
PRLVDNELAAIVDGDSLFITDKISNLEVNKLYIENATCFFESLEYTQDYITHAKALTAHVSSGTTGFYQKYLHKIDQIIK
YANDRIADLGLKQDDHLLVALSINHAFAFSYQILPALAIGLDITIIREFDAKLVARIINQNSVTALALLPIMYHFLLEQN
INKNHNLRYLSVAGDVASISLVTAVKDKLGLPLLNGIGMTEVFGYGQNISANTVNKVKIFSDTEVKILKFENSNYGKIFI
KNNMLPLNIETEWLETGDIGSFDHQSYELSFYGRYKDIIIKGGSNISPFELETAILKIPNIDNCIVTSKKDKIWGETVWA
YLVASQQYSLEFINNQLRNYIAEYKKLDGIIYIDEIPITTTGKTNRKKIKEMINHE

Sequences:

>Translated_456_residues
MIQLDKFLANIKNYGKNNYIFFEDKRYDYVEILQISYSLARFLESYGYRKVYSNLKNSPLLVSLYIASWIADIDLFVPIN
PRLVDNELAAIVDGDSLFITDKISNLEVNKLYIENATCFFESLEYTQDYITHAKALTAHVSSGTTGFYQKYLHKIDQIIK
YANDRIADLGLKQDDHLLVALSINHAFAFSYQILPALAIGLDITIIREFDAKLVARIINQNSVTALALLPIMYHFLLEQN
INKNHNLRYLSVAGDVASISLVTAVKDKLGLPLLNGIGMTEVFGYGQNISANTVNKVKIFSDTEVKILKFENSNYGKIFI
KNNMLPLNIETEWLETGDIGSFDHQSYELSFYGRYKDIIIKGGSNISPFELETAILKIPNIDNCIVTSKKDKIWGETVWA
YLVASQQYSLEFINNQLRNYIAEYKKLDGIIYIDEIPITTTGKTNRKKIKEMINHE
>Mature_456_residues
MIQLDKFLANIKNYGKNNYIFFEDKRYDYVEILQISYSLARFLESYGYRKVYSNLKNSPLLVSLYIASWIADIDLFVPIN
PRLVDNELAAIVDGDSLFITDKISNLEVNKLYIENATCFFESLEYTQDYITHAKALTAHVSSGTTGFYQKYLHKIDQIIK
YANDRIADLGLKQDDHLLVALSINHAFAFSYQILPALAIGLDITIIREFDAKLVARIINQNSVTALALLPIMYHFLLEQN
INKNHNLRYLSVAGDVASISLVTAVKDKLGLPLLNGIGMTEVFGYGQNISANTVNKVKIFSDTEVKILKFENSNYGKIFI
KNNMLPLNIETEWLETGDIGSFDHQSYELSFYGRYKDIIIKGGSNISPFELETAILKIPNIDNCIVTSKKDKIWGETVWA
YLVASQQYSLEFINNQLRNYIAEYKKLDGIIYIDEIPITTTGKTNRKKIKEMINHE

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI122937307, Length=378, Percent_Identity=25.1322751322751, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI42544132, Length=281, Percent_Identity=24.1992882562278, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI156151445, Length=372, Percent_Identity=22.5806451612903, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI115511026, Length=350, Percent_Identity=24.2857142857143, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI145693145, Length=352, Percent_Identity=25, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786810, Length=346, Percent_Identity=23.9884393063584, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI32563687, Length=454, Percent_Identity=24.0088105726872, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17558820, Length=469, Percent_Identity=25.1599147121535, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17557194, Length=307, Percent_Identity=27.0358306188925, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538037, Length=306, Percent_Identity=25.4901960784314, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25141359, Length=421, Percent_Identity=23.5154394299287, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=121, Percent_Identity=33.8842975206612, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21355181, Length=350, Percent_Identity=23.7142857142857, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24648260, Length=340, Percent_Identity=21.7647058823529, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 52060; Mature: 52060

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQLDKFLANIKNYGKNNYIFFEDKRYDYVEILQISYSLARFLESYGYRKVYSNLKNSPL
CCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCE
LVSLYIASWIADIDLFVPINPRLVDNELAAIVDGDSLFITDKISNLEVNKLYIENATCFF
EEEHHHHHHHHCCEEEEECCCEEECCCEEEEEECCEEEEEECCCCEEEEEEEEECCHHHH
ESLEYTQDYITHAKALTAHVSSGTTGFYQKYLHKIDQIIKYANDRIADLGLKQDDHLLVA
HHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEE
LSINHAFAFSYQILPALAIGLDITIIREFDAKLVARIINQNSVTALALLPIMYHFLLEQN
EEECCEEEEHHHHHHHHHHCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
INKNHNLRYLSVAGDVASISLVTAVKDKLGLPLLNGIGMTEVFGYGQNISANTVNKVKIF
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEE
SDTEVKILKFENSNYGKIFIKNNMLPLNIETEWLETGDIGSFDHQSYELSFYGRYKDIII
CCCCEEEEEEECCCCCEEEEECCEEEEEEEEEEECCCCCCCCCCCCEEEEEEECEEEEEE
KGGSNISPFELETAILKIPNIDNCIVTSKKDKIWGETVWAYLVASQQYSLEFINNQLRNY
ECCCCCCCEEEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IAEYKKLDGIIYIDEIPITTTGKTNRKKIKEMINHE
HHHHHHCCCEEEEECCCEECCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MIQLDKFLANIKNYGKNNYIFFEDKRYDYVEILQISYSLARFLESYGYRKVYSNLKNSPL
CCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCE
LVSLYIASWIADIDLFVPINPRLVDNELAAIVDGDSLFITDKISNLEVNKLYIENATCFF
EEEHHHHHHHHCCEEEEECCCEEECCCEEEEEECCEEEEEECCCCEEEEEEEEECCHHHH
ESLEYTQDYITHAKALTAHVSSGTTGFYQKYLHKIDQIIKYANDRIADLGLKQDDHLLVA
HHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEE
LSINHAFAFSYQILPALAIGLDITIIREFDAKLVARIINQNSVTALALLPIMYHFLLEQN
EEECCEEEEHHHHHHHHHHCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
INKNHNLRYLSVAGDVASISLVTAVKDKLGLPLLNGIGMTEVFGYGQNISANTVNKVKIF
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEE
SDTEVKILKFENSNYGKIFIKNNMLPLNIETEWLETGDIGSFDHQSYELSFYGRYKDIII
CCCCEEEEEEECCCCCEEEEECCEEEEEEEEEEECCCCCCCCCCCCEEEEEEECEEEEEE
KGGSNISPFELETAILKIPNIDNCIVTSKKDKIWGETVWAYLVASQQYSLEFINNQLRNY
ECCCCCCCEEEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IAEYKKLDGIIYIDEIPITTTGKTNRKKIKEMINHE
HHHHHHCCCEEEEECCCEECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]