Click here to switch to the map view.

The map label for this gene is rlmL [H]

Identifier: 134301838

GI number: 134301838

Start: 794362

End: 796515

Strand: Direct

Name: rlmL [H]

Synonym: FTW_0816

Alternate gene names: 134301838

Gene position: 794362-796515 (Clockwise)

Preceding gene: 134301837

Following gene: 134301839

Centisome position: 41.84

GC content: 31.2

Gene sequence:

>2154_bases
ATGCAAAAATTTACTTTTTTCGTTAGCTGTGCCAAAGGTATTGAGCTCCTACTCAAAGATGAGCTAGAAAGATTAGGTAT
ATCTTCTCAAGAAAAACTCGCTGGTGTAGAGTTTGAAGGCTCTATTAAAGATGCATATAAGGTATGTATATATTCTTATT
TAGCTAGTCAAGTTATGCTAAAAGTAGCTACTGATAAAGTCATAAATCAACAAGATTTATATGAATTTATATCATCTATA
AACTGGATGGATTATTTTGCTGTTGATAAAACTTTTAAAATTATAATTTCCGGTAAACATTATGATTTTAATAATACAAT
GTTTGTTTCCCAAAAGACCAAAGATGCTATTGTTGACCAGTTTAGAAATGTTACAAATCAACGCCCGAATATAGATACAG
AAAATCCTGATAATGTCATTAAACTTCACTTACACAAACAGTTTGTAAATGTATTCTTGTGCTTAAATATTGATAGCTTA
CACAAACGCAGTTATCGTCAGTTTCAGGGACAAGCGCCGCTAAAAGAATCATTAGCAGCTGCAATACTTATCAAAGCAGG
GTGGTTAGAAGAGCTAAAAAAACATCAACCAATATTGATTGATCCAATGTGTGGTTCTGGGACTATACTTATCGAAGCTG
CTCTTATGGCAAAAAATATCGCTCCGGTGCTGCTTAATAAAGAATTTAAAATATTTAACTCAAAGTTTCATAATCAAGAG
TTGTGGGATAATTTATTAGAAATAGCAAAAAACTCACAAAAAGTTACTAATGCAATTATTTGTGGGTTTGATATTGATAA
TAATGTTTTGGATAAAGCACAGAGAAATATTTACCAAGCTGGTGTTGAAGATGTTGTAACTGTTAAACGCCAAGATATTC
GTGACCTTGAAAATGAGTTTGAAAGTGAAGGCTTGATAGTTACAAATCCTCCATATGGTGAGCGATTATATGGTGATCAA
CTTGATGAACTTTTGGATATTTTTAATGGCTTTGGTGATAGATTATCGCAAGATTTTTATGGCTGGAAAGTAGCTGTTTT
GACAAGCTTTGCTGACTCAATCAAAGAGATGCAACTGCGTACGACTGAACGTAACAAATTTTATAATGGTGCTATTGAAA
CTATCCTTTATCAGTTTGAGATAAATGAACATGCTAAATTTAAACATGAAACTCAGCTTGAGAAAAATATCCGTATAGCC
GAAGCAAGTGCACAGAAGTCTGATGAACATATAGATTTTGCAAACAAGCTCAAAAAGAATCTCAAAAGTTTAAAGCCATG
GCTCAAGCAAACAGGTCTCGAGTGTTATAGGTTATATGATGCAGATATTCCAACTTTTGCGGTTGCAGTTGATGTATATA
GTGAGCATATTTTTTTACAAGAATATCGCGCCGATGCAACTATAGATCAAAATATTGCTAAACAAAGGTTCTATCAAGCT
ATATATCAGATACATAAAACACTTGATATAAAGTATGAAAATATCCATACACGAGTCCGTCAACGTCAAAAAGGCAAAGA
GCAGTATCAAAAAGAAAATGATAAAAATAAATTTCATATTATCAATGAATTTGATGCTAAGTTTTATGTCAATTTTGATG
ATTATTTAGATACCGGTATATTCTTAGATCATCGCAAAATTAGGCAGCTAGTTGCAAAAGCTGCAAAAAACAAAACTTTA
TTAAATCTATTTAGCTATACATGTACAGCAAGTGTTCATGCTGCTTTGAAAGGTGCAAAAACTACAAGTGTAGATATGTC
TAATACTTATCTTGAATGGGGTAAGAATAATTTCACACTAAACAATATCGATGCTAAAAAACATAGTTTTATCCAAGCAG
ATTGTATAAGTTGGCTAAAAACTAATAAAGACAAGTTTGATGTGATATTTTTAGATCCACCAACTTTCTCAAATTCAAAG
CGTATGGATGATATTTTAGATATTCAAAGGGATCATGAATTGTTGATAAATTTAGCAATGGATTCTCTTAAGAAAGATGG
GATACTTTATTTCTCAAACAATTATCGTCGCTTTAAAATGTCACCACAAATATTAGAAAAATTTAATTGCGAAAATATTG
ATAAAATCTGTTTATCGCGTGATTTCTTATCTAATAAAAATATTCATAACTGTTGGGAAATTAAATATAAATAA

Upstream 100 bases:

>100_bases
GGACTAAAGGAAAACAAAAAGATTAGCTTCTAATACTGATTGTTATTTATGTTAATTAATATATCATGTTAAGACATATT
CTATAATTTTATATTTTCTC

Downstream 100 bases:

>100_bases
AACTATCTCAGAATCTAGTATTTTAAAGTCTGTAATATTTTAGCTAAGCTAATCTATCTGTGATAAAATTTGCATAGCAA
TAAAAAAATATATTTAATTA

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 717; Mature: 717

Protein sequence:

>717_residues
MQKFTFFVSCAKGIELLLKDELERLGISSQEKLAGVEFEGSIKDAYKVCIYSYLASQVMLKVATDKVINQQDLYEFISSI
NWMDYFAVDKTFKIIISGKHYDFNNTMFVSQKTKDAIVDQFRNVTNQRPNIDTENPDNVIKLHLHKQFVNVFLCLNIDSL
HKRSYRQFQGQAPLKESLAAAILIKAGWLEELKKHQPILIDPMCGSGTILIEAALMAKNIAPVLLNKEFKIFNSKFHNQE
LWDNLLEIAKNSQKVTNAIICGFDIDNNVLDKAQRNIYQAGVEDVVTVKRQDIRDLENEFESEGLIVTNPPYGERLYGDQ
LDELLDIFNGFGDRLSQDFYGWKVAVLTSFADSIKEMQLRTTERNKFYNGAIETILYQFEINEHAKFKHETQLEKNIRIA
EASAQKSDEHIDFANKLKKNLKSLKPWLKQTGLECYRLYDADIPTFAVAVDVYSEHIFLQEYRADATIDQNIAKQRFYQA
IYQIHKTLDIKYENIHTRVRQRQKGKEQYQKENDKNKFHIINEFDAKFYVNFDDYLDTGIFLDHRKIRQLVAKAAKNKTL
LNLFSYTCTASVHAALKGAKTTSVDMSNTYLEWGKNNFTLNNIDAKKHSFIQADCISWLKTNKDKFDVIFLDPPTFSNSK
RMDDILDIQRDHELLINLAMDSLKKDGILYFSNNYRRFKMSPQILEKFNCENIDKICLSRDFLSNKNIHNCWEIKYK

Sequences:

>Translated_717_residues
MQKFTFFVSCAKGIELLLKDELERLGISSQEKLAGVEFEGSIKDAYKVCIYSYLASQVMLKVATDKVINQQDLYEFISSI
NWMDYFAVDKTFKIIISGKHYDFNNTMFVSQKTKDAIVDQFRNVTNQRPNIDTENPDNVIKLHLHKQFVNVFLCLNIDSL
HKRSYRQFQGQAPLKESLAAAILIKAGWLEELKKHQPILIDPMCGSGTILIEAALMAKNIAPVLLNKEFKIFNSKFHNQE
LWDNLLEIAKNSQKVTNAIICGFDIDNNVLDKAQRNIYQAGVEDVVTVKRQDIRDLENEFESEGLIVTNPPYGERLYGDQ
LDELLDIFNGFGDRLSQDFYGWKVAVLTSFADSIKEMQLRTTERNKFYNGAIETILYQFEINEHAKFKHETQLEKNIRIA
EASAQKSDEHIDFANKLKKNLKSLKPWLKQTGLECYRLYDADIPTFAVAVDVYSEHIFLQEYRADATIDQNIAKQRFYQA
IYQIHKTLDIKYENIHTRVRQRQKGKEQYQKENDKNKFHIINEFDAKFYVNFDDYLDTGIFLDHRKIRQLVAKAAKNKTL
LNLFSYTCTASVHAALKGAKTTSVDMSNTYLEWGKNNFTLNNIDAKKHSFIQADCISWLKTNKDKFDVIFLDPPTFSNSK
RMDDILDIQRDHELLINLAMDSLKKDGILYFSNNYRRFKMSPQILEKFNCENIDKICLSRDFLSNKNIHNCWEIKYK
>Mature_717_residues
MQKFTFFVSCAKGIELLLKDELERLGISSQEKLAGVEFEGSIKDAYKVCIYSYLASQVMLKVATDKVINQQDLYEFISSI
NWMDYFAVDKTFKIIISGKHYDFNNTMFVSQKTKDAIVDQFRNVTNQRPNIDTENPDNVIKLHLHKQFVNVFLCLNIDSL
HKRSYRQFQGQAPLKESLAAAILIKAGWLEELKKHQPILIDPMCGSGTILIEAALMAKNIAPVLLNKEFKIFNSKFHNQE
LWDNLLEIAKNSQKVTNAIICGFDIDNNVLDKAQRNIYQAGVEDVVTVKRQDIRDLENEFESEGLIVTNPPYGERLYGDQ
LDELLDIFNGFGDRLSQDFYGWKVAVLTSFADSIKEMQLRTTERNKFYNGAIETILYQFEINEHAKFKHETQLEKNIRIA
EASAQKSDEHIDFANKLKKNLKSLKPWLKQTGLECYRLYDADIPTFAVAVDVYSEHIFLQEYRADATIDQNIAKQRFYQA
IYQIHKTLDIKYENIHTRVRQRQKGKEQYQKENDKNKFHIINEFDAKFYVNFDDYLDTGIFLDHRKIRQLVAKAAKNKTL
LNLFSYTCTASVHAALKGAKTTSVDMSNTYLEWGKNNFTLNNIDAKKHSFIQADCISWLKTNKDKFDVIFLDPPTFSNSK
RMDDILDIQRDHELLINLAMDSLKKDGILYFSNNYRRFKMSPQILEKFNCENIDKICLSRDFLSNKNIHNCWEIKYK

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=724, Percent_Identity=39.3646408839779, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI145693111, Length=275, Percent_Identity=29.4545454545455, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 83471; Mature: 83471

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKFTFFVSCAKGIELLLKDELERLGISSQEKLAGVEFEGSIKDAYKVCIYSYLASQVML
CCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
KVATDKVINQQDLYEFISSINWMDYFAVDKTFKIIISGKHYDFNNTMFVSQKTKDAIVDQ
HHHHHHHCCHHHHHHHHHHCCCHHHEECCCEEEEEEECCEECCCCEEEEECCHHHHHHHH
FRNVTNQRPNIDTENPDNVIKLHLHKQFVNVFLCLNIDSLHKRSYRQFQGQAPLKESLAA
HHHHCCCCCCCCCCCCCCEEEEEEHHHHHHEEEEECCHHHHHHHHHHHCCCCCHHHHHHH
AILIKAGWLEELKKHQPILIDPMCGSGTILIEAALMAKNIAPVLLNKEFKIFNSKFHNQE
HHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEHHHHHCCCCHHHCCCCHHHHHHHCCCHH
LWDNLLEIAKNSQKVTNAIICGFDIDNNVLDKAQRNIYQAGVEDVVTVKRQDIRDLENEF
HHHHHHHHHHCCHHHCEEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
ESEGLIVTNPPYGERLYGDQLDELLDIFNGFGDRLSQDFYGWKVAVLTSFADSIKEMQLR
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TTERNKFYNGAIETILYQFEINEHAKFKHETQLEKNIRIAEASAQKSDEHIDFANKLKKN
HHHCCHHHCCHHHHEEEEEECCCCCCCCHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHH
LKSLKPWLKQTGLECYRLYDADIPTFAVAVDVYSEHIFLQEYRADATIDQNIAKQRFYQA
HHHHHHHHHHCCCCEEEEECCCCCHHEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
IYQIHKTLDIKYENIHTRVRQRQKGKEQYQKENDKNKFHIINEFDAKFYVNFDDYLDTGI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEHHHHCCCE
FLDHRKIRQLVAKAAKNKTLLNLFSYTCTASVHAALKGAKTTSVDMSNTYLEWGKNNFTL
EECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHCCCCCCEE
NNIDAKKHSFIQADCISWLKTNKDKFDVIFLDPPTFSNSKRMDDILDIQRDHELLINLAM
ECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DSLKKDGILYFSNNYRRFKMSPQILEKFNCENIDKICLSRDFLSNKNIHNCWEIKYK
HHHCCCCEEEEECCCEEEECCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEC
>Mature Secondary Structure
MQKFTFFVSCAKGIELLLKDELERLGISSQEKLAGVEFEGSIKDAYKVCIYSYLASQVML
CCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
KVATDKVINQQDLYEFISSINWMDYFAVDKTFKIIISGKHYDFNNTMFVSQKTKDAIVDQ
HHHHHHHCCHHHHHHHHHHCCCHHHEECCCEEEEEEECCEECCCCEEEEECCHHHHHHHH
FRNVTNQRPNIDTENPDNVIKLHLHKQFVNVFLCLNIDSLHKRSYRQFQGQAPLKESLAA
HHHHCCCCCCCCCCCCCCEEEEEEHHHHHHEEEEECCHHHHHHHHHHHCCCCCHHHHHHH
AILIKAGWLEELKKHQPILIDPMCGSGTILIEAALMAKNIAPVLLNKEFKIFNSKFHNQE
HHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEHHHHHCCCCHHHCCCCHHHHHHHCCCHH
LWDNLLEIAKNSQKVTNAIICGFDIDNNVLDKAQRNIYQAGVEDVVTVKRQDIRDLENEF
HHHHHHHHHHCCHHHCEEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
ESEGLIVTNPPYGERLYGDQLDELLDIFNGFGDRLSQDFYGWKVAVLTSFADSIKEMQLR
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TTERNKFYNGAIETILYQFEINEHAKFKHETQLEKNIRIAEASAQKSDEHIDFANKLKKN
HHHCCHHHCCHHHHEEEEEECCCCCCCCHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHH
LKSLKPWLKQTGLECYRLYDADIPTFAVAVDVYSEHIFLQEYRADATIDQNIAKQRFYQA
HHHHHHHHHHCCCCEEEEECCCCCHHEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
IYQIHKTLDIKYENIHTRVRQRQKGKEQYQKENDKNKFHIINEFDAKFYVNFDDYLDTGI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEHHHHCCCE
FLDHRKIRQLVAKAAKNKTLLNLFSYTCTASVHAALKGAKTTSVDMSNTYLEWGKNNFTL
EECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHCCCCCCEE
NNIDAKKHSFIQADCISWLKTNKDKFDVIFLDPPTFSNSKRMDDILDIQRDHELLINLAM
ECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DSLKKDGILYFSNNYRRFKMSPQILEKFNCENIDKICLSRDFLSNKNIHNCWEIKYK
HHHCCCCEEEEECCCEEEECCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA