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The map label for this gene is aceF [H]

Identifier: 134301833

GI number: 134301833

Start: 786947

End: 788842

Strand: Direct

Name: aceF [H]

Synonym: FTW_0809

Alternate gene names: 134301833

Gene position: 786947-788842 (Clockwise)

Preceding gene: 134301832

Following gene: 134301834

Centisome position: 41.45

GC content: 38.4

Gene sequence:

>1896_bases
ATGTCTATAGAGATAGTTAAAGTCCCTGATATTGGGGATTATGATAACGTTGATGTGATCGAGGTTAATGTTGCTGTAGG
TGATGTTATTGCTGAAGAAGATTCACTAATTACGTTAGAAACAGATAAAGCAAGTATGGAGGTTCCATCTCCATTTGCAG
GTAAGATCACTAAATTAACTGTAAAAGTTGGTGATAAAGTTTCTCAGGGAACTGCAATAATGGAAGTTGAGGTTGAGAGT
GCTGCTGATCAAGCTGCTACTACACAATCACAACCTCAAACAACTAGTTCAGCTCCTGTAGCTGCTACGACGAACCAGAT
TGTTGATGTCGAGGTTCCAGATATTGGCGACTATGATAGTGTTGATGTGATTGAGGTGTCTGTAAAAGTAGGTGATGAGA
TAGCAGAAGAGGATTCGCTAATTACGCTTGAAACAGATAAGGCTAGTATGGAAGTACCATCGCCTGTAGCGGGTAAGGTT
GTTGAAGTTATTACTAAGGTTGGTGACAAGGTTTCGCAAGGTAGCTTGATTTTAAAGGTTGAAACAGGCTCTAGTGCACA
AGCTCCAGCTCAAGAACAGTCACAACAATCTGCACCAGTTAAATCCGCTGCTGAAGAAATTATTGATGTAAAAGTTCCTG
ATATCGGTGATTACGATAGTGTTGATGTGATTGAAGTATCTGTAGCTGTTGGTGATAAGATTGAAGAAGAAGATTCTTTG
ATTACATTAGAAACTGATAAGGCAAGCATGGAGGTTCCGTCTCCAGTTGCTGGTGAAGTTGTTGAAATAATCACAAAAGT
TGGTGATAAAGTTTCACAAGGTAGTTTAATTCTTAAGGTCAAAACACAAGGTTCAGCACCGGTAGAGCTAACTAGTTCAC
AACCAGCGTCAGCTAAACAAGAGCAAGCTAAACAGCAAGCTGCTACACCTGCTGCTCCAACGCCAGCGTCAAGTTCGGTA
AATGAGTATGCTGTAGATAATTCTAATGCACATGCGTCTCCTGCAGTGAGAAAGCTAGCACGAATTCTAAATATTGATCT
AAGTAAGGTTAAAGCTACAGGGCGTAAAGGTCGTGTAACAAAAGAAGATTGTTATAACTATATTAAGCATGCTGTCACAC
AAGTTCAAACTGGTAAGGTCGCTGCTAGTGGTAGTGGTTTAGATCTTTTAGATGATCCTGTTGTTGATTTTGCTAAGTTT
GGTGAGATTGAAACTCAACCATTATCAAGAATTAACAAGATTAGTGCTAAGAATTTACATCGTAACTGGGTGAAGATTCC
TCATGTTACATTCTATGATGATGCGGATGTCACAGACTTAGAAGAGTTCAGAAATGCTAAGAAAGCCTTTGCTGAGAAAA
AAGGTATTAAGATTACACCTTTATCATTCTTGGTTAAAGCTGCTGCAGTTGCATTACAAGAGTTCCCAAGATTTAATAGC
TCATTATCAAATGATGGTGAGAACTTAATTATCAAGAAGTATTATAATATTGGTTTTGCTGCAGATACTCCAGCTGGTTT
AATGGTTCCAGTTGTCAAAGATGCTGATAAAAAGGGTATCATTGAAATATCAAAAGATATTATGGAGTTGGCTGGCAAAG
CTCGTGATGGTAAACTTGGCGCAAAGGATATGACAGGTGCTACATTTACTATCTCAAGCTTAGGCGTGTTAGGTACTACG
TCATTTACGCCTATTATAAATATGCCAGAGGTGGCTATTATGGGTGTATCTAAGACAGCAGTGAAGCCTATTTGGAATGG
TAAAGAGTTTATTCCTAGAACTATGCTACCATTATCATTATCTACAGATCATAGAGTGATAGATGGCGCATTAGCAGCTA
AATTCTTAACTAGATATTGTCAGATTTTATCTGATTTACGTGAAATCATAATGTAA

Upstream 100 bases:

>100_bases
GCAAAGTTGAGGCTAGCGAAGTTAAAGCTGCGATTGAGAAGTACAATATAGATCCAGAGCGTATGGCACCTTTTTATAGC
TAATTGGTAAGGAGATATTA

Downstream 100 bases:

>100_bases
GCGGAGTTTTAAAATGAGTGATATTAAAACACAAGTTGTAGTTTTAGGTAGTGGTCCTGGTGGATATAGTGCGGCTTTTA
GAGCAGCTGACTTAGGATTA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MSIEIVKVPDIGDYDNVDVIEVNVAVGDVIAEEDSLITLETDKASMEVPSPFAGKITKLTVKVGDKVSQGTAIMEVEVES
AADQAATTQSQPQTTSSAPVAATTNQIVDVEVPDIGDYDSVDVIEVSVKVGDEIAEEDSLITLETDKASMEVPSPVAGKV
VEVITKVGDKVSQGSLILKVETGSSAQAPAQEQSQQSAPVKSAAEEIIDVKVPDIGDYDSVDVIEVSVAVGDKIEEEDSL
ITLETDKASMEVPSPVAGEVVEIITKVGDKVSQGSLILKVKTQGSAPVELTSSQPASAKQEQAKQQAATPAAPTPASSSV
NEYAVDNSNAHASPAVRKLARILNIDLSKVKATGRKGRVTKEDCYNYIKHAVTQVQTGKVAASGSGLDLLDDPVVDFAKF
GEIETQPLSRINKISAKNLHRNWVKIPHVTFYDDADVTDLEEFRNAKKAFAEKKGIKITPLSFLVKAAAVALQEFPRFNS
SLSNDGENLIIKKYYNIGFAADTPAGLMVPVVKDADKKGIIEISKDIMELAGKARDGKLGAKDMTGATFTISSLGVLGTT
SFTPIINMPEVAIMGVSKTAVKPIWNGKEFIPRTMLPLSLSTDHRVIDGALAAKFLTRYCQILSDLREIIM

Sequences:

>Translated_631_residues
MSIEIVKVPDIGDYDNVDVIEVNVAVGDVIAEEDSLITLETDKASMEVPSPFAGKITKLTVKVGDKVSQGTAIMEVEVES
AADQAATTQSQPQTTSSAPVAATTNQIVDVEVPDIGDYDSVDVIEVSVKVGDEIAEEDSLITLETDKASMEVPSPVAGKV
VEVITKVGDKVSQGSLILKVETGSSAQAPAQEQSQQSAPVKSAAEEIIDVKVPDIGDYDSVDVIEVSVAVGDKIEEEDSL
ITLETDKASMEVPSPVAGEVVEIITKVGDKVSQGSLILKVKTQGSAPVELTSSQPASAKQEQAKQQAATPAAPTPASSSV
NEYAVDNSNAHASPAVRKLARILNIDLSKVKATGRKGRVTKEDCYNYIKHAVTQVQTGKVAASGSGLDLLDDPVVDFAKF
GEIETQPLSRINKISAKNLHRNWVKIPHVTFYDDADVTDLEEFRNAKKAFAEKKGIKITPLSFLVKAAAVALQEFPRFNS
SLSNDGENLIIKKYYNIGFAADTPAGLMVPVVKDADKKGIIEISKDIMELAGKARDGKLGAKDMTGATFTISSLGVLGTT
SFTPIINMPEVAIMGVSKTAVKPIWNGKEFIPRTMLPLSLSTDHRVIDGALAAKFLTRYCQILSDLREIIM
>Mature_630_residues
SIEIVKVPDIGDYDNVDVIEVNVAVGDVIAEEDSLITLETDKASMEVPSPFAGKITKLTVKVGDKVSQGTAIMEVEVESA
ADQAATTQSQPQTTSSAPVAATTNQIVDVEVPDIGDYDSVDVIEVSVKVGDEIAEEDSLITLETDKASMEVPSPVAGKVV
EVITKVGDKVSQGSLILKVETGSSAQAPAQEQSQQSAPVKSAAEEIIDVKVPDIGDYDSVDVIEVSVAVGDKIEEEDSLI
TLETDKASMEVPSPVAGEVVEIITKVGDKVSQGSLILKVKTQGSAPVELTSSQPASAKQEQAKQQAATPAAPTPASSSVN
EYAVDNSNAHASPAVRKLARILNIDLSKVKATGRKGRVTKEDCYNYIKHAVTQVQTGKVAASGSGLDLLDDPVVDFAKFG
EIETQPLSRINKISAKNLHRNWVKIPHVTFYDDADVTDLEEFRNAKKAFAEKKGIKITPLSFLVKAAAVALQEFPRFNSS
LSNDGENLIIKKYYNIGFAADTPAGLMVPVVKDADKKGIIEISKDIMELAGKARDGKLGAKDMTGATFTISSLGVLGTTS
FTPIINMPEVAIMGVSKTAVKPIWNGKEFIPRTMLPLSLSTDHRVIDGALAAKFLTRYCQILSDLREIIM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=449, Percent_Identity=29.6213808463252, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI31711992, Length=411, Percent_Identity=32.1167883211679, Blast_Score=168, Evalue=2e-41,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=34.2222222222222, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI203098816, Length=430, Percent_Identity=28.3720930232558, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI203098753, Length=430, Percent_Identity=28.3720930232558, Blast_Score=130, Evalue=6e-30,
Organism=Homo sapiens, GI260898739, Length=146, Percent_Identity=35.6164383561644, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786305, Length=635, Percent_Identity=51.9685039370079, Blast_Score=556, Evalue=1e-159,
Organism=Escherichia coli, GI1786946, Length=432, Percent_Identity=31.4814814814815, Blast_Score=190, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17537937, Length=431, Percent_Identity=29.6983758700696, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17560088, Length=438, Percent_Identity=29.6803652968037, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI25146366, Length=206, Percent_Identity=39.3203883495146, Blast_Score=135, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=220, Percent_Identity=33.6363636363636, Blast_Score=112, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6320352, Length=410, Percent_Identity=30.7317073170732, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6324258, Length=428, Percent_Identity=26.6355140186916, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI18859875, Length=452, Percent_Identity=32.0796460176991, Blast_Score=192, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=33.0472103004292, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI20129315, Length=230, Percent_Identity=33.0434782608696, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24645909, Length=191, Percent_Identity=37.696335078534, Blast_Score=123, Evalue=4e-28,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 67195; Mature: 67064

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEIVKVPDIGDYDNVDVIEVNVAVGDVIAEEDSLITLETDKASMEVPSPFAGKITKLT
CCEEEEECCCCCCCCCEEEEEEEEEECCEEECCCCEEEEECCCCCCCCCCCCCCCEEEEE
VKVGDKVSQGTAIMEVEVESAADQAATTQSQPQTTSSAPVAATTNQIVDVEVPDIGDYDS
EEECCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCC
VDVIEVSVKVGDEIAEEDSLITLETDKASMEVPSPVAGKVVEVITKVGDKVSQGSLILKV
CEEEEEEEECCCHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
ETGSSAQAPAQEQSQQSAPVKSAAEEIIDVKVPDIGDYDSVDVIEVSVAVGDKIEEEDSL
ECCCCCCCCCHHHHHHCCCHHHHHHHHHEEECCCCCCCCCCEEEEEEEECCCCCCCCCCE
ITLETDKASMEVPSPVAGEVVEIITKVGDKVSQGSLILKVKTQGSAPVELTSSQPASAKQ
EEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEECCCCCCCHHH
EQAKQQAATPAAPTPASSSVNEYAVDNSNAHASPAVRKLARILNIDLSKVKATGRKGRVT
HHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCC
KEDCYNYIKHAVTQVQTGKVAASGSGLDLLDDPVVDFAKFGEIETQPLSRINKISAKNLH
HHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RNWVKIPHVTFYDDADVTDLEEFRNAKKAFAEKKGIKITPLSFLVKAAAVALQEFPRFNS
CCCEECCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHCCCCCC
SLSNDGENLIIKKYYNIGFAADTPAGLMVPVVKDADKKGIIEISKDIMELAGKARDGKLG
CCCCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCC
AKDMTGATFTISSLGVLGTTSFTPIINMPEVAIMGVSKTAVKPIWNGKEFIPRTMLPLSL
CCCCCCCEEEEECCCEECCCCCCCCCCCCCEEEEECCHHHCCCCCCCCHHCCCCCCCEEC
STDHRVIDGALAAKFLTRYCQILSDLREIIM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SIEIVKVPDIGDYDNVDVIEVNVAVGDVIAEEDSLITLETDKASMEVPSPFAGKITKLT
CEEEEECCCCCCCCCEEEEEEEEEECCEEECCCCEEEEECCCCCCCCCCCCCCCEEEEE
VKVGDKVSQGTAIMEVEVESAADQAATTQSQPQTTSSAPVAATTNQIVDVEVPDIGDYDS
EEECCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCC
VDVIEVSVKVGDEIAEEDSLITLETDKASMEVPSPVAGKVVEVITKVGDKVSQGSLILKV
CEEEEEEEECCCHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
ETGSSAQAPAQEQSQQSAPVKSAAEEIIDVKVPDIGDYDSVDVIEVSVAVGDKIEEEDSL
ECCCCCCCCCHHHHHHCCCHHHHHHHHHEEECCCCCCCCCCEEEEEEEECCCCCCCCCCE
ITLETDKASMEVPSPVAGEVVEIITKVGDKVSQGSLILKVKTQGSAPVELTSSQPASAKQ
EEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEECCCCCCCHHH
EQAKQQAATPAAPTPASSSVNEYAVDNSNAHASPAVRKLARILNIDLSKVKATGRKGRVT
HHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCC
KEDCYNYIKHAVTQVQTGKVAASGSGLDLLDDPVVDFAKFGEIETQPLSRINKISAKNLH
HHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RNWVKIPHVTFYDDADVTDLEEFRNAKKAFAEKKGIKITPLSFLVKAAAVALQEFPRFNS
CCCEECCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHCCCCCC
SLSNDGENLIIKKYYNIGFAADTPAGLMVPVVKDADKKGIIEISKDIMELAGKARDGKLG
CCCCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCC
AKDMTGATFTISSLGVLGTTSFTPIINMPEVAIMGVSKTAVKPIWNGKEFIPRTMLPLSL
CCCCCCCEEEEECCCEECCCCCCCCCCCCCEEEEECCHHHCCCCCCCCHHCCCCCCCEEC
STDHRVIDGALAAKFLTRYCQILSDLREIIM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]