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The map label for this gene is gloA [H]

Identifier: 134301769

GI number: 134301769

Start: 713678

End: 714061

Strand: Direct

Name: gloA [H]

Synonym: FTW_0734

Alternate gene names: 134301769

Gene position: 713678-714061 (Clockwise)

Preceding gene: 134301767

Following gene: 134301770

Centisome position: 37.59

GC content: 33.85

Gene sequence:

>384_bases
ATGCGTTTTGCTCATGTAATGTTACGGGTAAAAGACTTAGATAAGTCTATCGATTTTTATACAAATGTACTATGGATGAC
AGTGCAGAAAAAAATTGATAATACAGAGTATAAGTATACTTTAGCATTTTTAGGTTATGGAGATATCTCTAGCCATACAG
TTCTAGAGTTAACATATAATTGGGGCGAGCATGAGTATGATCATAGTAATGCTTTTGGACATTTATGTATGCAAGTAGAG
GATGTCTACAAAGCTTGTGATGATGTCAAAGCAAAAGGTGGAGTTGTAACTCGTGAGGCAGGTCCTGTAAAAGGTGGCAC
TCAAATTATTGCATTTATAAAAGATCCAGATGGTTATCAAATAGAACTTATTGAAAAAGCTTAA

Upstream 100 bases:

>100_bases
TTTGTTAACCCTGGATAGTTGCGAACAGGGGATTAAAAATCTGCTATAATATAACAAAATAGTTTATCAATAACAAATAA
ATTTTTTATAAGGAGACTAG

Downstream 100 bases:

>100_bases
TGAATAAAGATAGATTAAATATATTAGTTATAAGAGGCTATTACAGTAAGGGTGATAGTGTTGAGCATATCGATGTTGAT
GGTATAAAAGTTAAGGTAAC

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MRFAHVMLRVKDLDKSIDFYTNVLWMTVQKKIDNTEYKYTLAFLGYGDISSHTVLELTYNWGEHEYDHSNAFGHLCMQVE
DVYKACDDVKAKGGVVTREAGPVKGGTQIIAFIKDPDGYQIELIEKA

Sequences:

>Translated_127_residues
MRFAHVMLRVKDLDKSIDFYTNVLWMTVQKKIDNTEYKYTLAFLGYGDISSHTVLELTYNWGEHEYDHSNAFGHLCMQVE
DVYKACDDVKAKGGVVTREAGPVKGGTQIIAFIKDPDGYQIELIEKA
>Mature_127_residues
MRFAHVMLRVKDLDKSIDFYTNVLWMTVQKKIDNTEYKYTLAFLGYGDISSHTVLELTYNWGEHEYDHSNAFGHLCMQVE
DVYKACDDVKAKGGVVTREAGPVKGGTQIIAFIKDPDGYQIELIEKA

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=144, Percent_Identity=38.8888888888889, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787940, Length=126, Percent_Identity=56.3492063492063, Blast_Score=161, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=35.9712230215827, Blast_Score=91, Evalue=6e-20,
Organism=Drosophila melanogaster, GI20129743, Length=146, Percent_Identity=37.6712328767123, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14500; Mature: 14500

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFAHVMLRVKDLDKSIDFYTNVLWMTVQKKIDNTEYKYTLAFLGYGDISSHTVLELTYN
CCHHHEEEEEHHCCCCHHHHHHHHHEEEEHHCCCCCEEEEEEEEECCCCCCCEEEEEEEC
WGEHEYDHSNAFGHLCMQVEDVYKACDDVKAKGGVVTREAGPVKGGTQIIAFIKDPDGYQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCE
IELIEKA
EEEEECC
>Mature Secondary Structure
MRFAHVMLRVKDLDKSIDFYTNVLWMTVQKKIDNTEYKYTLAFLGYGDISSHTVLELTYN
CCHHHEEEEEHHCCCCHHHHHHHHHEEEEHHCCCCCEEEEEEEEECCCCCCCEEEEEEEC
WGEHEYDHSNAFGHLCMQVEDVYKACDDVKAKGGVVTREAGPVKGGTQIIAFIKDPDGYQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCE
IELIEKA
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]