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The map label for this gene is thyA [H]

Identifier: 134301752

GI number: 134301752

Start: 697277

End: 698101

Strand: Reverse

Name: thyA [H]

Synonym: FTW_0717

Alternate gene names: 134301752

Gene position: 698101-697277 (Counterclockwise)

Preceding gene: 134301753

Following gene: 134301751

Centisome position: 36.77

GC content: 34.91

Gene sequence:

>825_bases
ATGCGAGAATATCTAAATTTCCTTAAGTATATCAAAGAAAATGGCGTTTTAAAGGGTGATAGAACCGGTACTGGAACAAG
AAGTATCTTTGGCTATCAAATGCGTTTTGATCTTCAAAAAGGCTTCCCATTAGTAACTACAAAAAAAATTCATATTCCTA
GTGTTGTTCATGAATTATTATGGTTTTTAAGTGGTAGTACAAATATCAAATATCTTAATGATAACAATGTTAGAATTTGG
AATGAGTGGGCAACTGTAGATGGCGAACTTGGTCCTATTTATGGCAAGCAGTGGCGTGATTTTAATGGTCAAGGTATAGA
TCAAATTGCCGATGTAATTCAGATGCTAAAAACAAACCCAAACTCACGTAGGATTCTAGTTTCAGCGTGGAATCCATGTG
TTGTTCCATCAGAAAAAATCTCTCCACAAGAAAATGTTGTAAAAGGTAATTCAGCCCTACCACCATGCCATGCAATGTTT
CAGTTCTATGTAGCAAATAATAAACTATCATGCATGCTTACGCAAAGAAGTGCTGATGCTTTTCTTGGAGTGCCATTTAA
CATTGCAAGTTATTCACTACTGACACATATGGTTGCACAACAATGTAATCTTGATGTTGGTGAGTTCATCTGGTCAGGTG
GTGATTGTCATATTTACAATAATCATATTGAGCAAGTCAATGAACAGCTTAGTCGTGAGCCTTTAGCTTTACCAACACTA
AAGATTTTAAGAAAACCAAACTCCATCTTTGATTATAAATACGAAGATTTTGAATTTGAGAACTATAACCATCACCCTGC
CATCAAAGCAAAAATATCTGTATAA

Upstream 100 bases:

>100_bases
TCTTTAATTGGATGACTATGGGGCAAATATTATCAATCCCAATGATATTACTAGGAGCGGTAATTTTAATCGCAGTATTT
ATTAAAACAAGGAAGAATAA

Downstream 100 bases:

>100_bases
GGATGCCCAAAATGAGCCAGCTATCTCTTAATAAAAAGAAAATCCCAATTCTTCTCTTAGAAGGTATACACTCAAATGCA
GTTGAATCATTCAAAGCTGC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MREYLNFLKYIKENGVLKGDRTGTGTRSIFGYQMRFDLQKGFPLVTTKKIHIPSVVHELLWFLSGSTNIKYLNDNNVRIW
NEWATVDGELGPIYGKQWRDFNGQGIDQIADVIQMLKTNPNSRRILVSAWNPCVVPSEKISPQENVVKGNSALPPCHAMF
QFYVANNKLSCMLTQRSADAFLGVPFNIASYSLLTHMVAQQCNLDVGEFIWSGGDCHIYNNHIEQVNEQLSREPLALPTL
KILRKPNSIFDYKYEDFEFENYNHHPAIKAKISV

Sequences:

>Translated_274_residues
MREYLNFLKYIKENGVLKGDRTGTGTRSIFGYQMRFDLQKGFPLVTTKKIHIPSVVHELLWFLSGSTNIKYLNDNNVRIW
NEWATVDGELGPIYGKQWRDFNGQGIDQIADVIQMLKTNPNSRRILVSAWNPCVVPSEKISPQENVVKGNSALPPCHAMF
QFYVANNKLSCMLTQRSADAFLGVPFNIASYSLLTHMVAQQCNLDVGEFIWSGGDCHIYNNHIEQVNEQLSREPLALPTL
KILRKPNSIFDYKYEDFEFENYNHHPAIKAKISV
>Mature_274_residues
MREYLNFLKYIKENGVLKGDRTGTGTRSIFGYQMRFDLQKGFPLVTTKKIHIPSVVHELLWFLSGSTNIKYLNDNNVRIW
NEWATVDGELGPIYGKQWRDFNGQGIDQIADVIQMLKTNPNSRRILVSAWNPCVVPSEKISPQENVVKGNSALPPCHAMF
QFYVANNKLSCMLTQRSADAFLGVPFNIASYSLLTHMVAQQCNLDVGEFIWSGGDCHIYNNHIEQVNEQLSREPLALPTL
KILRKPNSIFDYKYEDFEFENYNHHPAIKAKISV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=295, Percent_Identity=48.4745762711864, Blast_Score=277, Evalue=9e-75,
Organism=Escherichia coli, GI1789191, Length=277, Percent_Identity=59.2057761732852, Blast_Score=350, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI71993377, Length=296, Percent_Identity=43.2432432432432, Blast_Score=251, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6324648, Length=241, Percent_Identity=42.7385892116183, Blast_Score=192, Evalue=7e-50,
Organism=Drosophila melanogaster, GI17137556, Length=296, Percent_Identity=44.5945945945946, Blast_Score=263, Evalue=7e-71,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 31376; Mature: 31376

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREYLNFLKYIKENGVLKGDRTGTGTRSIFGYQMRFDLQKGFPLVTTKKIHIPSVVHELL
CHHHHHHHHHHHHCCCEECCCCCCCCHHEEEEEEEEECCCCCCEEEEEEEECHHHHHHHH
WFLSGSTNIKYLNDNNVRIWNEWATVDGELGPIYGKQWRDFNGQGIDQIADVIQMLKTNP
HHHCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
NSRRILVSAWNPCVVPSEKISPQENVVKGNSALPPCHAMFQFYVANNKLSCMLTQRSADA
CCCEEEEEECCCCCCCCCCCCCHHHEECCCCCCCHHHHHHEEEEECCCEEEEEEECCCCE
FLGVPFNIASYSLLTHMVAQQCNLDVGEFIWSGGDCHIYNNHIEQVNEQLSREPLALPTL
EECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEECHHHHHHHHHHCCCCCCCHHH
KILRKPNSIFDYKYEDFEFENYNHHPAIKAKISV
HHHCCCCCCCCCCCCCEECCCCCCCCCEEEEECC
>Mature Secondary Structure
MREYLNFLKYIKENGVLKGDRTGTGTRSIFGYQMRFDLQKGFPLVTTKKIHIPSVVHELL
CHHHHHHHHHHHHCCCEECCCCCCCCHHEEEEEEEEECCCCCCEEEEEEEECHHHHHHHH
WFLSGSTNIKYLNDNNVRIWNEWATVDGELGPIYGKQWRDFNGQGIDQIADVIQMLKTNP
HHHCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
NSRRILVSAWNPCVVPSEKISPQENVVKGNSALPPCHAMFQFYVANNKLSCMLTQRSADA
CCCEEEEEECCCCCCCCCCCCCHHHEECCCCCCCHHHHHHEEEEECCCEEEEEEECCCCE
FLGVPFNIASYSLLTHMVAQQCNLDVGEFIWSGGDCHIYNNHIEQVNEQLSREPLALPTL
EECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEECHHHHHHHHHHCCCCCCCHHH
KILRKPNSIFDYKYEDFEFENYNHHPAIKAKISV
HHHCCCCCCCCCCCCCEECCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA