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The map label for this gene is glyA [H]

Identifier: 134301741

GI number: 134301741

Start: 683322

End: 684575

Strand: Reverse

Name: glyA [H]

Synonym: FTW_0703

Alternate gene names: 134301741

Gene position: 684575-683322 (Counterclockwise)

Preceding gene: 134301743

Following gene: 134301739

Centisome position: 36.06

GC content: 36.68

Gene sequence:

>1254_bases
ATGTTCAGCTTTGAAAAAAATAGCCTAAAAAATACCGACAAAGAGATTTTTGATGCTATAGAACTTGAAGTTAAAAGACA
ACATGAACATGTTGAACTTATAGCATCAGAAAACTATGCAAGTCCTGCAGTAATGGAGGCACAAGGGTCACAACTTACAA
ACAAATATGCCGAAGGCTATCATGGTAAAAGATATTATGGTGGTTGTGAATTTGTTGATATCGCTGAGAAACTTGCCATA
GAGAGAGCACAGCAACTATTTGGGGTTGATTATGCAAATGTTCAACCACATTCAGGCTCTCAAGCAAATGCCGCTGTATA
TAATGCCGTTTTAAAACCAGGTGATACTGTTCTTGGTATGGATCTAGGTGCTGGTGGACACCTAACACATGGTAGCAAAG
TTAATTTCTCTGGAAAAATTTATAATTCTATCCAATACGGCTTAGATGAAAATGGTGATATAGACTACAAGCAAGTAGCA
CAACTAGCTAAAGAGCACAAACCAAAAATGATAATAGCCGGCTTCTCAGCATTTTCTGGAATAATTAACTGGCAAAAGTT
CAGAGAAATAGCAGACTCCGTAGATGCCGTACTTATGGCAGATATAGCTCATGTAGCAGGTCTAGTAGCAGCTGGAGTAT
ACCCTAACCCATTCCCATATGTTTATGTTGCAACTACAACTACTCACAAGACATTAAGGGGACCTAGAGGTGGATTAATA
CTTTGTAATAATAATCCGGAACTTGCAAAAAAATTCCAATCAGCTATTTTCCCTGGTATTCAAGGTGGTCCTTTAATGCA
TGTAATAGCAGCTAAAGCAGTTGCATTTAAAGAAGCATTAGAACCAAGTTTTGTAGACTATCAAAAACAAGTATTAAAAA
ATGCTAAGGCTATGGAAAAAGTTTTAAAACAACGTGGTATTAATATAATCTCTGGTGGAACTAGTAATCATCTACTTTTA
CTTGATATTACAAATACTGGTTTCTCTGGTAAAGAAGCTGAGGCGGCTCTAGGTAGAGCAAATATCACTGTTAATAAAAA
CTCTATCCCAAATGATCCCCGTTCTCCTTTTGTCACTAGCGGATTAAGGATTGGAAGCCCTGCTATAACGACGAGAGGTT
TCAAAGAGAAAGAATGTGAGTTAGTCGCTAACTTATTAGCAGATGTAGTATTTAATTGTGGTGATGAAAAAGTTGAAAAT
GAAACTGCTGCTAAAGTTTTAGATCTTTGTGATAAGTTCCCTGTTTACAAGTAA

Upstream 100 bases:

>100_bases
TTTTATTTTTAATTTGTTTCGATATATATATAATATAAGTTATAATTTTAAACATTAAATTTATTTTATCCACCTAAAAA
ATATCAGGAGACTACCAAAT

Downstream 100 bases:

>100_bases
ATCTTAAATCTCATAAATTGTTAATTTATTTTTTAATCTCTAGTTTTAATTAACATTCAATTCATCATAAAATTAATTAA
ATATTAATCACCAAAATCCT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MFSFEKNSLKNTDKEIFDAIELEVKRQHEHVELIASENYASPAVMEAQGSQLTNKYAEGYHGKRYYGGCEFVDIAEKLAI
ERAQQLFGVDYANVQPHSGSQANAAVYNAVLKPGDTVLGMDLGAGGHLTHGSKVNFSGKIYNSIQYGLDENGDIDYKQVA
QLAKEHKPKMIIAGFSAFSGIINWQKFREIADSVDAVLMADIAHVAGLVAAGVYPNPFPYVYVATTTTHKTLRGPRGGLI
LCNNNPELAKKFQSAIFPGIQGGPLMHVIAAKAVAFKEALEPSFVDYQKQVLKNAKAMEKVLKQRGINIISGGTSNHLLL
LDITNTGFSGKEAEAALGRANITVNKNSIPNDPRSPFVTSGLRIGSPAITTRGFKEKECELVANLLADVVFNCGDEKVEN
ETAAKVLDLCDKFPVYK

Sequences:

>Translated_417_residues
MFSFEKNSLKNTDKEIFDAIELEVKRQHEHVELIASENYASPAVMEAQGSQLTNKYAEGYHGKRYYGGCEFVDIAEKLAI
ERAQQLFGVDYANVQPHSGSQANAAVYNAVLKPGDTVLGMDLGAGGHLTHGSKVNFSGKIYNSIQYGLDENGDIDYKQVA
QLAKEHKPKMIIAGFSAFSGIINWQKFREIADSVDAVLMADIAHVAGLVAAGVYPNPFPYVYVATTTTHKTLRGPRGGLI
LCNNNPELAKKFQSAIFPGIQGGPLMHVIAAKAVAFKEALEPSFVDYQKQVLKNAKAMEKVLKQRGINIISGGTSNHLLL
LDITNTGFSGKEAEAALGRANITVNKNSIPNDPRSPFVTSGLRIGSPAITTRGFKEKECELVANLLADVVFNCGDEKVEN
ETAAKVLDLCDKFPVYK
>Mature_417_residues
MFSFEKNSLKNTDKEIFDAIELEVKRQHEHVELIASENYASPAVMEAQGSQLTNKYAEGYHGKRYYGGCEFVDIAEKLAI
ERAQQLFGVDYANVQPHSGSQANAAVYNAVLKPGDTVLGMDLGAGGHLTHGSKVNFSGKIYNSIQYGLDENGDIDYKQVA
QLAKEHKPKMIIAGFSAFSGIINWQKFREIADSVDAVLMADIAHVAGLVAAGVYPNPFPYVYVATTTTHKTLRGPRGGLI
LCNNNPELAKKFQSAIFPGIQGGPLMHVIAAKAVAFKEALEPSFVDYQKQVLKNAKAMEKVLKQRGINIISGGTSNHLLL
LDITNTGFSGKEAEAALGRANITVNKNSIPNDPRSPFVTSGLRIGSPAITTRGFKEKECELVANLLADVVFNCGDEKVEN
ETAAKVLDLCDKFPVYK

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=421, Percent_Identity=45.8432304038005, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI261862352, Length=421, Percent_Identity=45.8432304038005, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI261862350, Length=421, Percent_Identity=45.8432304038005, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI261862348, Length=421, Percent_Identity=45.8432304038005, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI261862346, Length=420, Percent_Identity=44.7619047619048, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=45.6359102244389, Blast_Score=319, Evalue=3e-87,
Organism=Homo sapiens, GI22547189, Length=388, Percent_Identity=43.5567010309278, Blast_Score=287, Evalue=1e-77,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=66.1870503597122, Blast_Score=558, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI25144732, Length=406, Percent_Identity=45.8128078817734, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=406, Percent_Identity=45.8128078817734, Blast_Score=346, Evalue=1e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=44.6384039900249, Blast_Score=343, Evalue=3e-95,
Organism=Saccharomyces cerevisiae, GI6323087, Length=405, Percent_Identity=42.962962962963, Blast_Score=319, Evalue=6e-88,
Organism=Drosophila melanogaster, GI24640005, Length=425, Percent_Identity=44, Blast_Score=352, Evalue=4e-97,
Organism=Drosophila melanogaster, GI221329721, Length=425, Percent_Identity=44, Blast_Score=351, Evalue=6e-97,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45316; Mature: 45316

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSFEKNSLKNTDKEIFDAIELEVKRQHEHVELIASENYASPAVMEAQGSQLTNKYAEGY
CCCCCCCHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCHHHHHHHHCCC
HGKRYYGGCEFVDIAEKLAIERAQQLFGVDYANVQPHSGSQANAAVYNAVLKPGDTVLGM
CCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEE
DLGAGGHLTHGSKVNFSGKIYNSIQYGLDENGDIDYKQVAQLAKEHKPKMIIAGFSAFSG
EECCCCCCCCCCEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHH
IINWQKFREIADSVDAVLMADIAHVAGLVAAGVYPNPFPYVYVATTTTHKTLRGPRGGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCCCCCEE
LCNNNPELAKKFQSAIFPGIQGGPLMHVIAAKAVAFKEALEPSFVDYQKQVLKNAKAMEK
EECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VLKQRGINIISGGTSNHLLLLDITNTGFSGKEAEAALGRANITVNKNSIPNDPRSPFVTS
HHHHCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCCHHHC
GLRIGSPAITTRGFKEKECELVANLLADVVFNCGDEKVENETAAKVLDLCDKFPVYK
CEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFSFEKNSLKNTDKEIFDAIELEVKRQHEHVELIASENYASPAVMEAQGSQLTNKYAEGY
CCCCCCCHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCHHHHHHHHCCC
HGKRYYGGCEFVDIAEKLAIERAQQLFGVDYANVQPHSGSQANAAVYNAVLKPGDTVLGM
CCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEE
DLGAGGHLTHGSKVNFSGKIYNSIQYGLDENGDIDYKQVAQLAKEHKPKMIIAGFSAFSG
EECCCCCCCCCCEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHH
IINWQKFREIADSVDAVLMADIAHVAGLVAAGVYPNPFPYVYVATTTTHKTLRGPRGGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCCCCCEE
LCNNNPELAKKFQSAIFPGIQGGPLMHVIAAKAVAFKEALEPSFVDYQKQVLKNAKAMEK
EECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VLKQRGINIISGGTSNHLLLLDITNTGFSGKEAEAALGRANITVNKNSIPNDPRSPFVTS
HHHHCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCCHHHC
GLRIGSPAITTRGFKEKECELVANLLADVVFNCGDEKVENETAAKVLDLCDKFPVYK
CEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA