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The map label for this gene is glyS [H]

Identifier: 134301720

GI number: 134301720

Start: 655628

End: 657712

Strand: Direct

Name: glyS [H]

Synonym: FTW_0675

Alternate gene names: 134301720

Gene position: 655628-657712 (Clockwise)

Preceding gene: 134301715

Following gene: 134301721

Centisome position: 34.53

GC content: 33.19

Gene sequence:

>2085_bases
ATGAGTAAAATTACAAAAGATTTTTTATTTGAATTGGGTACTGAAGAGTTACCACCAAAAGCACTAAGAAATTTAGCCCA
GTCTTTATTAGTCAGTGTTGAAAGTCAGCTAAAAGAGGCAGAAGTTAGTTTTGGTGATACTAAATGGTTCGCTTCACCAA
GAAGGTTATCTTTTATAATCAAAGGTCTAGCAGAGTCTCAACAAGATGTAGTTATTGAAAAGCAAGGACCGTTGGTAAGT
ATTGCTTATAAGGAAGGTGAACCTACTCAAGTAGGTTTAGGTTTTGCAAAATCTTGTGGTGTTGAGCTTGATGAATTAGA
AAGGGTAGCTACACCAAAAGGTGATAAGCTTTTTTATAAAACAGTACAATCTGGTCAAGCAACAGTTAATTTATTGCAAG
AAATTATTACCAAAGCGCTCAAACAATTACCAATTTCAAAGATGATGCGTTGGGGCTCTTCAAGTGTTGAGTTTGTAAGA
CCTGTACATTGGGTATTAGCATTATATGGTAACGATGTTGTCGATATAGAAATTTTAGGACATAAAGCTGCTAATATTAC
ATATGGACACAGATTCCATCACCCTCAAGCTATAGTAATTGATAATATTAGTGATTATATCAAGCTACTTGCTGATGCTA
TGGTGATTGTAGATTGGCAACAGAGCAAACAAATGTTAGTTGAACAAGCTGAAAATATTGCTAAAGAAAATGATTATCAA
GTAGTTCTAGACGATGATTTAGTCGAAGAGGTTTGTGCTATAGTTGAGTACCCAAACGCAATGTTATGCAGTTTTAACAA
GGATTTCCTAAGAGTTCCTCAAGAAGCGTTAATATCTGCTATGGAAGAACATCAAAAGTGTTTTGCTCTATTGGATAATC
AGGGTGATTTGGTTGCTAATTTTATTACAATTTCAAATATCCAAAGTAAAAAGCCAGAACTAGTAACATCAGGTAATCAA
AAGGTAATGAATGCTAGGCTAGCAGATGCGGCTTTTTTCTATGATACTGATTTGAAGACTTCTTTAGAACAGTTGTTGCC
TAAGCTTGAGCATGTAACTTTCCAAAGTAAACTTGGTAATATGTATCAGAAAGCTCAAAGAATAGCTAATATAGCACAAC
AGTTAGCAGAACTAGGTAATTTTGATTCTCAACAAGCACATAGGGCGGGTCTTTTAGCTAAAGCTGACTTAATTTCAAAT
ATGGTGTTTGAGTTTACAGATTTACAAGGAATTATTGGTAAATATTACGCAAAAGCTCATGGTGAAACAGATACAGTAGC
CGAGGCAATAGAGCAACAATATTGGCCAAAGTATTCTGGAGCGGAGTTACCAAGAACTAATGTTGCTGCATGTGTAGCAT
TAGCTGAGAAATTAGATACTTTAGTAGGTATATTTGCAATTGGTCAGAAACCAACAGGAAATAAAGATCCATTTGCATTA
AGAAGATCAGCTATAGGTATCTTGCGCATACTAAGAGATACAAGTGTTGATATATCTTTAGAAAAGATTATTGATATAAC
TTTAGAAAGTTATAAAAAAATTAATAATTTAGAGTTTAATACTGATGTAAAAACAGAAGTAATATCTTTCTGTCTAGATA
GACTAAAAAACCTTTATAAAGAAGAGGGTATTGCTGTAGATATTTTTGAAGCTATTAATAATACTAATTATGATTCGATC
AAAGATTTTGCTGCGAGAGTAGAAGCTGTAACTAAGTTCTACAATTCAGATAAAGCTCAGAGTCTAATAGCTTCAAATAA
GCGTGTAGCTAATATTCTTAGCAAGAATGCTACAGATAAAGATTACTATTATAATATTGAACTTGCTAAAGCAGCTGCTA
ATGAATATGAGTTAGCACTTGCTTATAGTATCGAAGAGGTTGCTGCTGATCTTCATAAGTACCTTAATAATCGAGAATAT
AGTTATGCTCTAGAGCTGTTGACTTGTCTTGATAAAGTGATTAGTAAGTTTTTTGAGAATGTAATGGTAATTGATGAAGA
TATTAAGATAAGAGAAAATAGACTTGCTTTGCTTGTAAACCTGCATAAGATGTTCATTGGAATTGCTGATATCTCTAAAT
TATAG

Upstream 100 bases:

>100_bases
GGATCTCAATATAATTGATATTTGTCGTGAGATGCTGAAATTAATTTAGCATGACATTTTAGTGTTAATGAAAATAAACA
TTTATCTATAAGGTTTGTAA

Downstream 100 bases:

>100_bases
GTATATCGAAAATTACTAGATTTTATTCTAGATAGCTAGGCTATATTATGTAAAAAAATAGCCAAAGCTTATATATAGTT
AATCCGAAAGATATTTGTAG

Product: glycyl-tRNA synthetase, beta subunit

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MSKITKDFLFELGTEELPPKALRNLAQSLLVSVESQLKEAEVSFGDTKWFASPRRLSFIIKGLAESQQDVVIEKQGPLVS
IAYKEGEPTQVGLGFAKSCGVELDELERVATPKGDKLFYKTVQSGQATVNLLQEIITKALKQLPISKMMRWGSSSVEFVR
PVHWVLALYGNDVVDIEILGHKAANITYGHRFHHPQAIVIDNISDYIKLLADAMVIVDWQQSKQMLVEQAENIAKENDYQ
VVLDDDLVEEVCAIVEYPNAMLCSFNKDFLRVPQEALISAMEEHQKCFALLDNQGDLVANFITISNIQSKKPELVTSGNQ
KVMNARLADAAFFYDTDLKTSLEQLLPKLEHVTFQSKLGNMYQKAQRIANIAQQLAELGNFDSQQAHRAGLLAKADLISN
MVFEFTDLQGIIGKYYAKAHGETDTVAEAIEQQYWPKYSGAELPRTNVAACVALAEKLDTLVGIFAIGQKPTGNKDPFAL
RRSAIGILRILRDTSVDISLEKIIDITLESYKKINNLEFNTDVKTEVISFCLDRLKNLYKEEGIAVDIFEAINNTNYDSI
KDFAARVEAVTKFYNSDKAQSLIASNKRVANILSKNATDKDYYYNIELAKAAANEYELALAYSIEEVAADLHKYLNNREY
SYALELLTCLDKVISKFFENVMVIDEDIKIRENRLALLVNLHKMFIGIADISKL

Sequences:

>Translated_694_residues
MSKITKDFLFELGTEELPPKALRNLAQSLLVSVESQLKEAEVSFGDTKWFASPRRLSFIIKGLAESQQDVVIEKQGPLVS
IAYKEGEPTQVGLGFAKSCGVELDELERVATPKGDKLFYKTVQSGQATVNLLQEIITKALKQLPISKMMRWGSSSVEFVR
PVHWVLALYGNDVVDIEILGHKAANITYGHRFHHPQAIVIDNISDYIKLLADAMVIVDWQQSKQMLVEQAENIAKENDYQ
VVLDDDLVEEVCAIVEYPNAMLCSFNKDFLRVPQEALISAMEEHQKCFALLDNQGDLVANFITISNIQSKKPELVTSGNQ
KVMNARLADAAFFYDTDLKTSLEQLLPKLEHVTFQSKLGNMYQKAQRIANIAQQLAELGNFDSQQAHRAGLLAKADLISN
MVFEFTDLQGIIGKYYAKAHGETDTVAEAIEQQYWPKYSGAELPRTNVAACVALAEKLDTLVGIFAIGQKPTGNKDPFAL
RRSAIGILRILRDTSVDISLEKIIDITLESYKKINNLEFNTDVKTEVISFCLDRLKNLYKEEGIAVDIFEAINNTNYDSI
KDFAARVEAVTKFYNSDKAQSLIASNKRVANILSKNATDKDYYYNIELAKAAANEYELALAYSIEEVAADLHKYLNNREY
SYALELLTCLDKVISKFFENVMVIDEDIKIRENRLALLVNLHKMFIGIADISKL
>Mature_693_residues
SKITKDFLFELGTEELPPKALRNLAQSLLVSVESQLKEAEVSFGDTKWFASPRRLSFIIKGLAESQQDVVIEKQGPLVSI
AYKEGEPTQVGLGFAKSCGVELDELERVATPKGDKLFYKTVQSGQATVNLLQEIITKALKQLPISKMMRWGSSSVEFVRP
VHWVLALYGNDVVDIEILGHKAANITYGHRFHHPQAIVIDNISDYIKLLADAMVIVDWQQSKQMLVEQAENIAKENDYQV
VLDDDLVEEVCAIVEYPNAMLCSFNKDFLRVPQEALISAMEEHQKCFALLDNQGDLVANFITISNIQSKKPELVTSGNQK
VMNARLADAAFFYDTDLKTSLEQLLPKLEHVTFQSKLGNMYQKAQRIANIAQQLAELGNFDSQQAHRAGLLAKADLISNM
VFEFTDLQGIIGKYYAKAHGETDTVAEAIEQQYWPKYSGAELPRTNVAACVALAEKLDTLVGIFAIGQKPTGNKDPFALR
RSAIGILRILRDTSVDISLEKIIDITLESYKKINNLEFNTDVKTEVISFCLDRLKNLYKEEGIAVDIFEAINNTNYDSIK
DFAARVEAVTKFYNSDKAQSLIASNKRVANILSKNATDKDYYYNIELAKAAANEYELALAYSIEEVAADLHKYLNNREYS
YALELLTCLDKVISKFFENVMVIDEDIKIRENRLALLVNLHKMFIGIADISKL

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=691, Percent_Identity=41.6787264833575, Blast_Score=536, Evalue=1e-153,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 77953; Mature: 77822

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKITKDFLFELGTEELPPKALRNLAQSLLVSVESQLKEAEVSFGDTKWFASPRRLSFII
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHH
KGLAESQQDVVIEKQGPLVSIAYKEGEPTQVGLGFAKSCGVELDELERVATPKGDKLFYK
HHHHCCCCCEEEECCCCEEEEEECCCCCCEECCCHHHHCCCCHHHHHHHHCCCCCHHHHH
TVQSGQATVNLLQEIITKALKQLPISKMMRWGSSSVEFVRPVHWVLALYGNDVVDIEILG
HHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEC
HKAANITYGHRFHHPQAIVIDNISDYIKLLADAMVIVDWQQSKQMLVEQAENIAKENDYQ
CCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHCCCCCE
VVLDDDLVEEVCAIVEYPNAMLCSFNKDFLRVPQEALISAMEEHQKCFALLDNQGDLVAN
EEECHHHHHHHHHHHHCCCHHEECCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEH
FITISNIQSKKPELVTSGNQKVMNARLADAAFFYDTDLKTSLEQLLPKLEHVTFQSKLGN
HEEECCCCCCCCCCEECCCHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHH
MYQKAQRIANIAQQLAELGNFDSQQAHRAGLLAKADLISNMVFEFTDLQGIIGKYYAKAH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GETDTVAEAIEQQYWPKYSGAELPRTNVAACVALAEKLDTLVGIFAIGQKPTGNKDPFAL
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RRSAIGILRILRDTSVDISLEKIIDITLESYKKINNLEFNTDVKTEVISFCLDRLKNLYK
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
EEGIAVDIFEAINNTNYDSIKDFAARVEAVTKFYNSDKAQSLIASNKRVANILSKNATDK
HCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHCCCCCC
DYYYNIELAKAAANEYELALAYSIEEVAADLHKYLNNREYSYALELLTCLDKVISKFFEN
CEEEEEEEHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
VMVIDEDIKIRENRLALLVNLHKMFIGIADISKL
EEEECCCCHHHHCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKITKDFLFELGTEELPPKALRNLAQSLLVSVESQLKEAEVSFGDTKWFASPRRLSFII
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHH
KGLAESQQDVVIEKQGPLVSIAYKEGEPTQVGLGFAKSCGVELDELERVATPKGDKLFYK
HHHHCCCCCEEEECCCCEEEEEECCCCCCEECCCHHHHCCCCHHHHHHHHCCCCCHHHHH
TVQSGQATVNLLQEIITKALKQLPISKMMRWGSSSVEFVRPVHWVLALYGNDVVDIEILG
HHHCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEC
HKAANITYGHRFHHPQAIVIDNISDYIKLLADAMVIVDWQQSKQMLVEQAENIAKENDYQ
CCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHCCCCCE
VVLDDDLVEEVCAIVEYPNAMLCSFNKDFLRVPQEALISAMEEHQKCFALLDNQGDLVAN
EEECHHHHHHHHHHHHCCCHHEECCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEH
FITISNIQSKKPELVTSGNQKVMNARLADAAFFYDTDLKTSLEQLLPKLEHVTFQSKLGN
HEEECCCCCCCCCCEECCCHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHH
MYQKAQRIANIAQQLAELGNFDSQQAHRAGLLAKADLISNMVFEFTDLQGIIGKYYAKAH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GETDTVAEAIEQQYWPKYSGAELPRTNVAACVALAEKLDTLVGIFAIGQKPTGNKDPFAL
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RRSAIGILRILRDTSVDISLEKIIDITLESYKKINNLEFNTDVKTEVISFCLDRLKNLYK
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
EEGIAVDIFEAINNTNYDSIKDFAARVEAVTKFYNSDKAQSLIASNKRVANILSKNATDK
HCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHCCCCCC
DYYYNIELAKAAANEYELALAYSIEEVAADLHKYLNNREYSYALELLTCLDKVISKFFEN
CEEEEEEEHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
VMVIDEDIKIRENRLALLVNLHKMFIGIADISKL
EEEECCCCHHHHCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA