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The map label for this gene is recF

Identifier: 134301718

GI number: 134301718

Start: 653636

End: 654685

Strand: Reverse

Name: recF

Synonym: FTW_0673

Alternate gene names: 134301718

Gene position: 654685-653636 (Counterclockwise)

Preceding gene: 134301719

Following gene: 134301717

Centisome position: 34.48

GC content: 26.29

Gene sequence:

>1050_bases
ATGTATATATCTAACTTACGCTTACAAAATTTTAGAAATATACCTGCTAAAAGCTTTGATTTTAAAAATAGTATAAATTT
TATAGTTGGTAAAAATGGTTCAGGTAAAACCTCAATTCTTGAATCAATATATTTTTTATCACATAGTCGTTCTTTTCGTA
GCTCACAGTTAAATAGAATTATTAACCATAATGCTGATGAGTTTATAATCTATACTAAAGCTTATAATCCAGATGAAATT
ACTATCTCGCTATCACGCAAAAAAAATAGTAATAATATCTCAAAGCTAAATTTAGAAATACAAAAAAACCATACAGAAAT
AACTAGAAACTTACCCATTCAATTGATCAATCCCGAGAGTTTTAATATCATAAACTCTGGAGCACAACAAAGATGTAAAG
TGCTTGACTGGGGTGCTTTTTATCTTGATAAAACATTTTTAAAAATATGGCAACAAACTAAATTTCTCGTTAAACAAAGA
AATTCTGCTTTAAAACAAAACTATCCATACAGCTATATACTTAGTATAGATAAAAAACTCTGTGAGTTTGCTGAAATACT
TGACTATAAAAGACAAGCCTATTTTACAAAATTAAAACCTAAAATCTATGAAATATTATCGCATTTTAATCCAAACTTAC
AGCTTGATATAGATTACTTTCGAGGTTGGAACTTACATAAAAGTCTAGCACAAGTGCTAGAAGAATCTTTTAATTATGAC
AATAAATATAAAGTCACTAATCATGGTCCACATAAGGCTGATATAGTTCTAAGTGTAAGTCATAAACCTATCCAAGATAT
CTTTTCACGTGGACAACAAAAATTACTTATTTGTGCACTAAAATTAGCTCAGGGAGAAATTCATAATTCAGAAAATGACA
ATAAATGTATTTATTTAATAGATGATATAACTTCTGAATTAGATAGTATTCATACTCTTACTTTGTTTAATTATCTCAAA
CAACTTAAATCACAAGTTTTTATAACAACTACAGAAAAAAATAAAATTAATGAATTTATTGACACAAATAGCTATATATT
AGAAATATAA

Upstream 100 bases:

>100_bases
TATCCCTAAGATTTTTGAGTTATTAAGCAATATTTATCAACTAAATCCTGATATACTAAAACAACAAATTTATAATACTA
GCTTAGAATTTATCTAGAAA

Downstream 100 bases:

>100_bases
AACTTATAACAATTAAAAATCATGAAAAAACTCATAATTATGACTTTAACAATCCTACCTACTTTAGTAGCAGCAGATAA
AGTTTGCGATGGTAATACTT

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MYISNLRLQNFRNIPAKSFDFKNSINFIVGKNGSGKTSILESIYFLSHSRSFRSSQLNRIINHNADEFIIYTKAYNPDEI
TISLSRKKNSNNISKLNLEIQKNHTEITRNLPIQLINPESFNIINSGAQQRCKVLDWGAFYLDKTFLKIWQQTKFLVKQR
NSALKQNYPYSYILSIDKKLCEFAEILDYKRQAYFTKLKPKIYEILSHFNPNLQLDIDYFRGWNLHKSLAQVLEESFNYD
NKYKVTNHGPHKADIVLSVSHKPIQDIFSRGQQKLLICALKLAQGEIHNSENDNKCIYLIDDITSELDSIHTLTLFNYLK
QLKSQVFITTTEKNKINEFIDTNSYILEI

Sequences:

>Translated_349_residues
MYISNLRLQNFRNIPAKSFDFKNSINFIVGKNGSGKTSILESIYFLSHSRSFRSSQLNRIINHNADEFIIYTKAYNPDEI
TISLSRKKNSNNISKLNLEIQKNHTEITRNLPIQLINPESFNIINSGAQQRCKVLDWGAFYLDKTFLKIWQQTKFLVKQR
NSALKQNYPYSYILSIDKKLCEFAEILDYKRQAYFTKLKPKIYEILSHFNPNLQLDIDYFRGWNLHKSLAQVLEESFNYD
NKYKVTNHGPHKADIVLSVSHKPIQDIFSRGQQKLLICALKLAQGEIHNSENDNKCIYLIDDITSELDSIHTLTLFNYLK
QLKSQVFITTTEKNKINEFIDTNSYILEI
>Mature_349_residues
MYISNLRLQNFRNIPAKSFDFKNSINFIVGKNGSGKTSILESIYFLSHSRSFRSSQLNRIINHNADEFIIYTKAYNPDEI
TISLSRKKNSNNISKLNLEIQKNHTEITRNLPIQLINPESFNIINSGAQQRCKVLDWGAFYLDKTFLKIWQQTKFLVKQR
NSALKQNYPYSYILSIDKKLCEFAEILDYKRQAYFTKLKPKIYEILSHFNPNLQLDIDYFRGWNLHKSLAQVLEESFNYD
NKYKVTNHGPHKADIVLSVSHKPIQDIFSRGQQKLLICALKLAQGEIHNSENDNKCIYLIDDITSELDSIHTLTLFNYLK
QLKSQVFITTTEKNKINEFIDTNSYILEI

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=352, Percent_Identity=37.5, Blast_Score=267, Evalue=6e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_FRAT1 (Q14I72)

Other databases:

- EMBL:   AM286280
- RefSeq:   YP_666904.1
- ProteinModelPortal:   Q14I72
- STRING:   Q14I72
- GeneID:   4200603
- GenomeReviews:   AM286280_GR
- KEGG:   ftf:FTF0762c
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   FASSFLV
- PhylomeDB:   Q14I72
- ProtClustDB:   CLSK934664
- BioCyc:   FTUL393115:FTF0762C-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 40843; Mature: 40843

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYISNLRLQNFRNIPAKSFDFKNSINFIVGKNGSGKTSILESIYFLSHSRSFRSSQLNRI
CCCCCCCHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
INHNADEFIIYTKAYNPDEITISLSRKKNSNNISKLNLEIQKNHTEITRNLPIQLINPES
HCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCEEEEEEEECCCHHHHHCCCEEEECCCC
FNIINSGAQQRCKVLDWGAFYLDKTFLKIWQQTKFLVKQRNSALKQNYPYSYILSIDKKL
CCEECCCHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHH
CEFAEILDYKRQAYFTKLKPKIYEILSHFNPNLQLDIDYFRGWNLHKSLAQVLEESFNYD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHCCCC
NKYKVTNHGPHKADIVLSVSHKPIQDIFSRGQQKLLICALKLAQGEIHNSENDNKCIYLI
CCEEECCCCCCCEEEEEEECCCCHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCEEEEEE
DDITSELDSIHTLTLFNYLKQLKSQVFITTTEKNKINEFIDTNSYILEI
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MYISNLRLQNFRNIPAKSFDFKNSINFIVGKNGSGKTSILESIYFLSHSRSFRSSQLNRI
CCCCCCCHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
INHNADEFIIYTKAYNPDEITISLSRKKNSNNISKLNLEIQKNHTEITRNLPIQLINPES
HCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCEEEEEEEECCCHHHHHCCCEEEECCCC
FNIINSGAQQRCKVLDWGAFYLDKTFLKIWQQTKFLVKQRNSALKQNYPYSYILSIDKKL
CCEECCCHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHH
CEFAEILDYKRQAYFTKLKPKIYEILSHFNPNLQLDIDYFRGWNLHKSLAQVLEESFNYD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHCCCC
NKYKVTNHGPHKADIVLSVSHKPIQDIFSRGQQKLLICALKLAQGEIHNSENDNKCIYLI
CCEEECCCCCCCEEEEEEECCCCHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCEEEEEE
DDITSELDSIHTLTLFNYLKQLKSQVFITTTEKNKINEFIDTNSYILEI
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA