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The map label for this gene is tufA [H]

Identifier: 134301346

GI number: 134301346

Start: 234496

End: 235680

Strand: Direct

Name: tufA [H]

Synonym: FTW_0227

Alternate gene names: 134301346

Gene position: 234496-235680 (Clockwise)

Preceding gene: 134301345

Following gene: 162329653

Centisome position: 12.35

GC content: 41.86

Gene sequence:

>1185_bases
ATGGCTAAAGAAAAATTTGAGCGTTCGAAGCCGCATGTAAACGTAGGTACAATTGGTCACGTTGACCACGGTAAAACTAC
TCTTACAGCAGCTATTACAAAAGTTATGGCTGAGAAGAACGGTGGTATGGCGCGTAAATTTGATGAGATTGATAGTGCGC
CAGAAGAAAAAGCGCGTGGTATTACTATTAATACTTCTCACGTTGAGTACGAGTCTCCTAATAGACACTATGCTCACGTA
GACTGTCCAGGACACGCTGACTACGTTAAGAATATGATTACTGGTGCTGCACAGATGGATGGTGCTATTCTAGTATGTTC
TGCTGCGGATGGTCCTATGCCACAAACTCGTGAGCACATTCTGCTTTCTCGTCAAGTTGGTGTACCAAAAATCGTTGTTT
TCTTAAACAAGTGTGACATGGTTGATGATGAAGAGTTATTAGAGCTAGTTGAGATGGAAGTTCGTGAGCTTTTAGATCAG
TATGAGTTCCCAGGTGATGACACTCCAGTTATTATGGGTTCAGCTCTTAGAGCTATTGAAGGTGACGAAGCTTACGTTGA
GAAAATTGTTGAGCTAGTTCAAGCTATGGATGACTATATTCCTGCTCCTGAGCGTGATACTGAGAAGCCATTTATTCTTC
CGATCGAAGATGTATTCTCAATTTCAGGTCGTGGTACTGTTGTAACTGGTCGTATTGAGCGCGGTGTAGTTAACGTTGGT
GATGAAGTTGAAGTTGTTGGTATTCGTCCAACTCAAAAAACTACAGTAACTGGTGTGGAAATGTTCCGTAAGCTTTTAGA
TAGAGGGGAAGCTGGTGATAACGTTGGTATCCTAGTTCGTGGACTTAAGAGAGATGATGTTGAGCGTGGACAAGTATTAT
GTAAGCCAGGTTCAATTAAGCCACATACTAAGTTTGAAGCTGAGGTTTATGTATTATCTAAAGAAGAGGGTGGTAGACAT
ACTCCATTCTTCAAGGGATATAGACCACAATTCTACTTCCGTACTACAGACATTACTGGAGCTGTTGAGCTTCCAGAGGG
TGTAGAAATGGTTATGCCTGGTGATAACGTTAAGATGACTATCACTCTAATTAACCCAATCGCTATGGATGAAGGGTTAC
GTTTTGCAATCCGTGAGGGTGGTAGAACAGTAGGTGCTGGTGTTGTAGCTAAAATTATCGAGTAA

Upstream 100 bases:

>100_bases
AGAGCACTCCCTTGGTAAGGGAGAGGTCACCAGTTCAAGTCTGGTTGTGAGCACCATATTTGAAGTTATTGTTTTTTGTT
GAAAAAAAATGGAGTAAAAA

Downstream 100 bases:

>100_bases
TTGATAATTTAAATATTGTAGGTCAGTAGTTCAATTGGTAGAGCAGCGGTCTCCAAAACCGCAAGTTGGGGGTTCGAGTC
CCTCCTGGCCTGCCACATAA

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKEKFERSKPHVNVGTIGHVDHGKTTLTAAITKVMAEKNGGMARKFDEIDSAPEEKARGITINTSHVEYESPNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPKIVVFLNKCDMVDDEELLELVEMEVRELLDQ
YEFPGDDTPVIMGSALRAIEGDEAYVEKIVELVQAMDDYIPAPERDTEKPFILPIEDVFSISGRGTVVTGRIERGVVNVG
DEVEVVGIRPTQKTTVTGVEMFRKLLDRGEAGDNVGILVRGLKRDDVERGQVLCKPGSIKPHTKFEAEVYVLSKEEGGRH
TPFFKGYRPQFYFRTTDITGAVELPEGVEMVMPGDNVKMTITLINPIAMDEGLRFAIREGGRTVGAGVVAKIIE

Sequences:

>Translated_394_residues
MAKEKFERSKPHVNVGTIGHVDHGKTTLTAAITKVMAEKNGGMARKFDEIDSAPEEKARGITINTSHVEYESPNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPKIVVFLNKCDMVDDEELLELVEMEVRELLDQ
YEFPGDDTPVIMGSALRAIEGDEAYVEKIVELVQAMDDYIPAPERDTEKPFILPIEDVFSISGRGTVVTGRIERGVVNVG
DEVEVVGIRPTQKTTVTGVEMFRKLLDRGEAGDNVGILVRGLKRDDVERGQVLCKPGSIKPHTKFEAEVYVLSKEEGGRH
TPFFKGYRPQFYFRTTDITGAVELPEGVEMVMPGDNVKMTITLINPIAMDEGLRFAIREGGRTVGAGVVAKIIE
>Mature_393_residues
AKEKFERSKPHVNVGTIGHVDHGKTTLTAAITKVMAEKNGGMARKFDEIDSAPEEKARGITINTSHVEYESPNRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPKIVVFLNKCDMVDDEELLELVEMEVRELLDQY
EFPGDDTPVIMGSALRAIEGDEAYVEKIVELVQAMDDYIPAPERDTEKPFILPIEDVFSISGRGTVVTGRIERGVVNVGD
EVEVVGIRPTQKTTVTGVEMFRKLLDRGEAGDNVGILVRGLKRDDVERGQVLCKPGSIKPHTKFEAEVYVLSKEEGGRHT
PFFKGYRPQFYFRTTDITGAVELPEGVEMVMPGDNVKMTITLINPIAMDEGLRFAIREGGRTVGAGVVAKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=58.3756345177665, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=29.2517006802721, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=29.2517006802721, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4503475, Length=447, Percent_Identity=27.9642058165548, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI4503471, Length=447, Percent_Identity=28.6353467561521, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI54607086, Length=347, Percent_Identity=28.2420749279539, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194018522, Length=434, Percent_Identity=26.4976958525346, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI194018520, Length=434, Percent_Identity=26.4976958525346, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI194097354, Length=434, Percent_Identity=26.4976958525346, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI46094014, Length=434, Percent_Identity=27.1889400921659, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI4503507, Length=274, Percent_Identity=30.2919708029197, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI157426893, Length=303, Percent_Identity=25.4125412541254, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=34.0909090909091, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=80.4071246819338, Blast_Score=645, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=80.4071246819338, Blast_Score=645, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=377, Percent_Identity=29.4429708222812, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI48994988, Length=278, Percent_Identity=31.6546762589928, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789108, Length=156, Percent_Identity=32.0512820512821, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788922, Length=330, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=50.8771929824561, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=45.0777202072539, Blast_Score=348, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI17552884, Length=451, Percent_Identity=28.8248337028825, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=451, Percent_Identity=28.8248337028825, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI32566303, Length=433, Percent_Identity=27.9445727482679, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=298, Percent_Identity=29.1946308724832, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=450, Percent_Identity=26.2222222222222, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI115532067, Length=435, Percent_Identity=25.9770114942529, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=25.9770114942529, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=160, Percent_Identity=34.375, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17509919, Length=446, Percent_Identity=26.0089686098655, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=232, Percent_Identity=26.2931034482759, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=63.3838383838384, Blast_Score=523, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=30.1587301587302, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=30.1587301587302, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=462, Percent_Identity=21.8614718614719, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=29.2035398230088, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320863, Length=284, Percent_Identity=28.169014084507, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=280, Percent_Identity=27.5, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=140, Percent_Identity=32.8571428571429, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.0152671755725, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.0152671755725, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=49.4818652849741, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45553807, Length=459, Percent_Identity=28.322440087146, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI45553816, Length=459, Percent_Identity=28.322440087146, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24651721, Length=459, Percent_Identity=28.322440087146, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI17864154, Length=459, Percent_Identity=28.322440087146, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24652838, Length=458, Percent_Identity=28.82096069869, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI17137572, Length=458, Percent_Identity=28.82096069869, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI19922690, Length=302, Percent_Identity=28.8079470198675, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI17137380, Length=343, Percent_Identity=30.6122448979592, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI45550900, Length=447, Percent_Identity=26.6219239373602, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24647048, Length=367, Percent_Identity=26.9754768392371, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24647046, Length=367, Percent_Identity=26.9754768392371, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI28572034, Length=165, Percent_Identity=35.1515151515151, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43391; Mature: 43260

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERSKPHVNVGTIGHVDHGKTTLTAAITKVMAEKNGGMARKFDEIDSAPEEKARG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHCC
ITINTSHVEYESPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEECCEEEECCCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPKIVVFLNKCDMVDDEELLELVEMEVRELLDQYEFPGDDTPVIMGSALRAIE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHCC
GDEAYVEKIVELVQAMDDYIPAPERDTEKPFILPIEDVFSISGRGTVVTGRIERGVVNVG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEEEEECCCEECCC
DEVEVVGIRPTQKTTVTGVEMFRKLLDRGEAGDNVGILVRGLKRDDVERGQVLCKPGSIK
CCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCC
PHTKFEAEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDITGAVELPEGVEMVMPGDNVKMT
CCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCEEEE
ITLINPIAMDEGLRFAIREGGRTVGAGVVAKIIE
EEEECCEECCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFERSKPHVNVGTIGHVDHGKTTLTAAITKVMAEKNGGMARKFDEIDSAPEEKARG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHCC
ITINTSHVEYESPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEECCEEEECCCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLSRQVGVPKIVVFLNKCDMVDDEELLELVEMEVRELLDQYEFPGDDTPVIMGSALRAIE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHCC
GDEAYVEKIVELVQAMDDYIPAPERDTEKPFILPIEDVFSISGRGTVVTGRIERGVVNVG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEEEEECCCEECCC
DEVEVVGIRPTQKTTVTGVEMFRKLLDRGEAGDNVGILVRGLKRDDVERGQVLCKPGSIK
CCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCC
PHTKFEAEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDITGAVELPEGVEMVMPGDNVKMT
CCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCEEEE
ITLINPIAMDEGLRFAIREGGRTVGAGVVAKIIE
EEEECCEECCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA