Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 134301263

GI number: 134301263

Start: 134102

End: 135571

Strand: Direct

Name: nusA [H]

Synonym: FTW_0125

Alternate gene names: 134301263

Gene position: 134102-135571 (Clockwise)

Preceding gene: 134301262

Following gene: 134301264

Centisome position: 7.06

GC content: 34.42

Gene sequence:

>1470_bases
ATGAGCAAAGAATTATTGTTAGTATTAGAAACTGTAGCAAATGAAAAAGATATCTCAAAAGATCTTTTATTTGAAGCTAT
GGAAGAGGCTTTGGCTATTATAACTAAGAAAGAACTTGATGAGCATATGAATATCGAGGTTAAAATCGATAGAGTTACGG
GTGATTTCAAGGCTGATAGAGTTTGGCATATTGTTTCTGAAGATGAAGATTTAATTGATTATTCAAAAGAGCTTTATGAA
GATGTCGTTCAAGAAAAAGGCTATAATGTAAAAGCTGGCGATGTTATGCGTGAACCTGTTGAGGTCAAAGAATATGGGCG
TATAGCTGCGACAATGGCTAAGCAAATTCTAATGAAGAAAATCAAAAATTTTGAAAGAGAAAAGTCAGCTAGATTTTATC
AAAATAAAATTGGCGATATTGTTTACGGCGAGATAAAGCGCGCTACATATGAAATCTTAGTTGTTGATTTGGGCAATAAT
GCTGAGGGGATTTTACCTAAAAAAGATCTAATAGCTAGAGAAAGATATCGAGTTGGTGACAAAATAAGAGCATGTGTTGA
AGCTGTTGAGAGTGATGAGTCTGGCAAGCCAAATACGATTATGTTAAGTCGTTCAAGCAATACTATGCTTAAAGCTTTAT
TTAAGCTTGAGGTTCCGGAGGTTGAAGAAGAGCTTATTAATATAGTGAATGTAGTTCGTGAACCTGGTTTTAGGTCTAAA
GTAACTGTTAAAAGTAATGATCAAAGAATAGATCCTTGTGGTGCCTGTGTTGGTGTGAGAGGTTCTAGAATACATTCTAT
AATGAGTGAGCTAAATGGTGAGAAGGTTGATGTTATACTTTGGAATGAAGATATGGTTCAGTATGCAATTAACTCATTAT
CACCAGTTGATGCAGCAGACATCTTAGAAGTGAATGTTGATGAAGAAACTAATTCGATAGATATTGTTGTTAAGCAAGAA
AGTTTATCTAAAGCAATTGGTAAAAATGGTGTTAATGTAAGATTAGCAAGTTCGTTAATAGGTTGGAAAATTAATGTTCT
ATCAGATGCTGAGCAGGAAGAAAAACAAATGTCTATAGTAGAGAAATTTGTTGAAGCCTTAGATATAGATCACGATTTTG
CATTAGTATTGATTGAAGAGGGTATAGAGACTCTTGAAGATTTAGCATATTTAGATAGAGCGGAGCTTTTAGAAATTGAA
GGGTTTGATGAAGAGATCGTTGATGAGCTTCAAGAGAGAGCAAAAGCAGTTATTTTATCTCAAGCTTTAGGAGGTAAAAA
ACCAGCTCAAGATTTGTTAGATATGCAAGGTATGAGTCTGGATTTGGCTGAGCAGCTAGCGCAAAATAATATAGTTACTA
TGGAAGATCTAGCAGAGCTATCTGTAGATGAGTTGCTTGATATTGTGGATATAGATGAAGAGCAAGCAACTAATTTAATT
ATGCAAGCAAGAGCTCCTTGGTTTGAATAA

Upstream 100 bases:

>100_bases
TTCTAAACTTGGAAGATGGTAAAGAAATAAGTTTTGATTTTGACGAGCTTAAAAAACTAAGGGTATCACCTGATTTTAGT
TAGATAGAGGAATTTAAAAT

Downstream 100 bases:

>100_bases
TAAAAGTACATATTAGGAATAGTACAAAGGAGAGGGAAATGGCAGAGATTACAGTTGGGCAGTTAGCACAGCAAACAAAT
AAAGAAGTAGATGCACTACT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSKELLLVLETVANEKDISKDLLFEAMEEALAIITKKELDEHMNIEVKIDRVTGDFKADRVWHIVSEDEDLIDYSKELYE
DVVQEKGYNVKAGDVMREPVEVKEYGRIAATMAKQILMKKIKNFEREKSARFYQNKIGDIVYGEIKRATYEILVVDLGNN
AEGILPKKDLIARERYRVGDKIRACVEAVESDESGKPNTIMLSRSSNTMLKALFKLEVPEVEEELINIVNVVREPGFRSK
VTVKSNDQRIDPCGACVGVRGSRIHSIMSELNGEKVDVILWNEDMVQYAINSLSPVDAADILEVNVDEETNSIDIVVKQE
SLSKAIGKNGVNVRLASSLIGWKINVLSDAEQEEKQMSIVEKFVEALDIDHDFALVLIEEGIETLEDLAYLDRAELLEIE
GFDEEIVDELQERAKAVILSQALGGKKPAQDLLDMQGMSLDLAEQLAQNNIVTMEDLAELSVDELLDIVDIDEEQATNLI
MQARAPWFE

Sequences:

>Translated_489_residues
MSKELLLVLETVANEKDISKDLLFEAMEEALAIITKKELDEHMNIEVKIDRVTGDFKADRVWHIVSEDEDLIDYSKELYE
DVVQEKGYNVKAGDVMREPVEVKEYGRIAATMAKQILMKKIKNFEREKSARFYQNKIGDIVYGEIKRATYEILVVDLGNN
AEGILPKKDLIARERYRVGDKIRACVEAVESDESGKPNTIMLSRSSNTMLKALFKLEVPEVEEELINIVNVVREPGFRSK
VTVKSNDQRIDPCGACVGVRGSRIHSIMSELNGEKVDVILWNEDMVQYAINSLSPVDAADILEVNVDEETNSIDIVVKQE
SLSKAIGKNGVNVRLASSLIGWKINVLSDAEQEEKQMSIVEKFVEALDIDHDFALVLIEEGIETLEDLAYLDRAELLEIE
GFDEEIVDELQERAKAVILSQALGGKKPAQDLLDMQGMSLDLAEQLAQNNIVTMEDLAELSVDELLDIVDIDEEQATNLI
MQARAPWFE
>Mature_488_residues
SKELLLVLETVANEKDISKDLLFEAMEEALAIITKKELDEHMNIEVKIDRVTGDFKADRVWHIVSEDEDLIDYSKELYED
VVQEKGYNVKAGDVMREPVEVKEYGRIAATMAKQILMKKIKNFEREKSARFYQNKIGDIVYGEIKRATYEILVVDLGNNA
EGILPKKDLIARERYRVGDKIRACVEAVESDESGKPNTIMLSRSSNTMLKALFKLEVPEVEEELINIVNVVREPGFRSKV
TVKSNDQRIDPCGACVGVRGSRIHSIMSELNGEKVDVILWNEDMVQYAINSLSPVDAADILEVNVDEETNSIDIVVKQES
LSKAIGKNGVNVRLASSLIGWKINVLSDAEQEEKQMSIVEKFVEALDIDHDFALVLIEEGIETLEDLAYLDRAELLEIEG
FDEEIVDELQERAKAVILSQALGGKKPAQDLLDMQGMSLDLAEQLAQNNIVTMEDLAELSVDELLDIVDIDEEQATNLIM
QARAPWFE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=499, Percent_Identity=40.8817635270541, Blast_Score=397, Evalue=1e-111,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55108; Mature: 54977

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKELLLVLETVANEKDISKDLLFEAMEEALAIITKKELDEHMNIEVKIDRVTGDFKADR
CCCHHEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCC
VWHIVSEDEDLIDYSKELYEDVVQEKGYNVKAGDVMREPVEVKEYGRIAATMAKQILMKK
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHH
IKNFEREKSARFYQNKIGDIVYGEIKRATYEILVVDLGNNAEGILPKKDLIARERYRVGD
HHHHHHHHHHHHHHHHCCCHHHHHHHHCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
KIRACVEAVESDESGKPNTIMLSRSSNTMLKALFKLEVPEVEEELINIVNVVREPGFRSK
HHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCE
VTVKSNDQRIDPCGACVGVRGSRIHSIMSELNGEKVDVILWNEDMVQYAINSLSPVDAAD
EEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCCCCCCC
ILEVNVDEETNSIDIVVKQESLSKAIGKNGVNVRLASSLIGWKINVLSDAEQEEKQMSIV
EEEEECCCCCCCEEEEEEHHHHHHHHCCCCCEEEEECHHHCEEEEECCCCHHHHHHHHHH
EKFVEALDIDHDFALVLIEEGIETLEDLAYLDRAELLEIEGFDEEIVDELQERAKAVILS
HHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCHHHEEEECCCCHHHHHHHHHHHHHHHHH
QALGGKKPAQDLLDMQGMSLDLAEQLAQNNIVTMEDLAELSVDELLDIVDIDEEQATNLI
HHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCEEHHHHHHCCHHHHHHHHCCCHHHHHHHH
MQARAPWFE
HHCCCCCCC
>Mature Secondary Structure 
SKELLLVLETVANEKDISKDLLFEAMEEALAIITKKELDEHMNIEVKIDRVTGDFKADR
CCHHEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCC
VWHIVSEDEDLIDYSKELYEDVVQEKGYNVKAGDVMREPVEVKEYGRIAATMAKQILMKK
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHH
IKNFEREKSARFYQNKIGDIVYGEIKRATYEILVVDLGNNAEGILPKKDLIARERYRVGD
HHHHHHHHHHHHHHHHCCCHHHHHHHHCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
KIRACVEAVESDESGKPNTIMLSRSSNTMLKALFKLEVPEVEEELINIVNVVREPGFRSK
HHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCE
VTVKSNDQRIDPCGACVGVRGSRIHSIMSELNGEKVDVILWNEDMVQYAINSLSPVDAAD
EEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCCCCCCC
ILEVNVDEETNSIDIVVKQESLSKAIGKNGVNVRLASSLIGWKINVLSDAEQEEKQMSIV
EEEEECCCCCCCEEEEEEHHHHHHHHCCCCCEEEEECHHHCEEEEECCCCHHHHHHHHHH
EKFVEALDIDHDFALVLIEEGIETLEDLAYLDRAELLEIEGFDEEIVDELQERAKAVILS
HHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCHHHEEEECCCCHHHHHHHHHHHHHHHHH
QALGGKKPAQDLLDMQGMSLDLAEQLAQNNIVTMEDLAELSVDELLDIVDIDEEQATNLI
HHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCEEHHHHHHCCHHHHHHHHCCCHHHHHHHH
MQARAPWFE
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]