Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 134301232

GI number: 134301232

Start: 98075

End: 99373

Strand: Direct

Name: purB [H]

Synonym: FTW_0089

Alternate gene names: 134301232

Gene position: 98075-99373 (Clockwise)

Preceding gene: 134301229

Following gene: 134301233

Centisome position: 5.17

GC content: 35.49

Gene sequence:

>1299_bases
ATGATAAAAAGATATGACATAGCAAAAATCTCAAAAATTTGGGCAGATGAGAATAAATATGCAAAAATGTTAGAGGTTGA
GCTTGCGATTTTAGAGGCACTTGAAGATAGAATGGTACCTAAAGGTACAGCTGCGGAAATTCGTGCTAGAGCACAAATTA
GACCTGAGAGAGTTGATGAGATTGAGAAAGTTACAAAGCATGACATCATCGCATTTTGTACCTCTATTGCGGAGCAATTT
ACAGCTGAAACTGGCAAGTTTTTTCATTTTGGTGTTACATCTTCAGATATTATTGACTCTGCTCTTAGCCTACAAATTCA
TGATTCTATGAGCTATGTTATCAAAGATTTAGAGGCGCTTTGTGACTCGCTACTTACTAAGGCAGAAGAAACAAAAGAAA
TCATTACGATGGGTAGAAGTCATGGTATGTTTGCCGAACCGATGAGCTTTGGTCAGAAGTTTCTTGGTGCTTATGTTGAG
TTTAAGCGTAGGCTAAAAGATCTCAAAGATTTTCAAAAAGATGGTTTGACAGTACAGTTCTCAGGAGCTGTAGGTAACTA
TTGTATTTTAACTACAGAAGATGAGAAAAAAGCAGCTGATATTTTAGGTTTACCTGTTGAAGAAGTTTCTACTCAAGTTA
TCCCAAGAGATAGAATTGCTAAGTTGATATCTATCCACGGACTTATTGCTTCTGCTATAGAGAGATTAGCAGTTGAGATT
AGACATTTACATCGTAGTGATGTTTTTGAGGTATATGAAGGCTTCTCTAAAGGGCAAAAAGGCTCATCAACTATGCCGCA
TAAGAAAAACCCAATTTCTACTGAAAACTTAACAGGTATGGCAAGAATGCTAAGATCTCATGTATCTATAGCATTAGAGA
ATTGTGTGCTATGGCATGAGCGAGATATTTCTCACTCTTCAGCAGAGCGTTTTTATCTGCCAGATAACTTTGGTATTATG
GTTTATGCTTTACGTAGAATGAAAAATACTATCGATAATCTCGTCGTGCAAAGAGATATTATCGAAGATAGGGTTAGAAG
TACTAGTGCTTATTTATCAAGTTTTTACTTACATTTCTTGGTAGCAAATACGCCATTTATGCGTGAAGATTGCTACAAGA
TTGTTCAGCAAGTTGCTTTTGATCTTAAACAAGGAGAATCTTTCTCGAAGAAATTACAAAAAGTTATGCACGATGAGCAT
AATATTATTTTAGATATTCCAGAGATGGATTTTGAGGGTATCAAGAAAACTTACCTAAAAGAAATTGATCATGTTTTTGA
TAGATCTGTTAAGGCGTAA

Upstream 100 bases:

>100_bases
AAATACTTCATCATGTATTGCCATGCTTGTGCTTTAGCTCAGTTTTAGCTTATATACACTAAGTGTGCTTGCGGGTGTTT
TATGAATATAAGGTAGTAAA

Downstream 100 bases:

>100_bases
ATATGAAAAAAATATTATCTTTTCTTTTTATTTATGTATAATGTTGTTAAGTTTGGATGCCAAGAGCGACCTGAAATAAG
TAGTGTAGAAGAGCCAACAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIKRYDIAKISKIWADENKYAKMLEVELAILEALEDRMVPKGTAAEIRARAQIRPERVDEIEKVTKHDIIAFCTSIAEQF
TAETGKFFHFGVTSSDIIDSALSLQIHDSMSYVIKDLEALCDSLLTKAEETKEIITMGRSHGMFAEPMSFGQKFLGAYVE
FKRRLKDLKDFQKDGLTVQFSGAVGNYCILTTEDEKKAADILGLPVEEVSTQVIPRDRIAKLISIHGLIASAIERLAVEI
RHLHRSDVFEVYEGFSKGQKGSSTMPHKKNPISTENLTGMARMLRSHVSIALENCVLWHERDISHSSAERFYLPDNFGIM
VYALRRMKNTIDNLVVQRDIIEDRVRSTSAYLSSFYLHFLVANTPFMREDCYKIVQQVAFDLKQGESFSKKLQKVMHDEH
NIILDIPEMDFEGIKKTYLKEIDHVFDRSVKA

Sequences:

>Translated_432_residues
MIKRYDIAKISKIWADENKYAKMLEVELAILEALEDRMVPKGTAAEIRARAQIRPERVDEIEKVTKHDIIAFCTSIAEQF
TAETGKFFHFGVTSSDIIDSALSLQIHDSMSYVIKDLEALCDSLLTKAEETKEIITMGRSHGMFAEPMSFGQKFLGAYVE
FKRRLKDLKDFQKDGLTVQFSGAVGNYCILTTEDEKKAADILGLPVEEVSTQVIPRDRIAKLISIHGLIASAIERLAVEI
RHLHRSDVFEVYEGFSKGQKGSSTMPHKKNPISTENLTGMARMLRSHVSIALENCVLWHERDISHSSAERFYLPDNFGIM
VYALRRMKNTIDNLVVQRDIIEDRVRSTSAYLSSFYLHFLVANTPFMREDCYKIVQQVAFDLKQGESFSKKLQKVMHDEH
NIILDIPEMDFEGIKKTYLKEIDHVFDRSVKA
>Mature_432_residues
MIKRYDIAKISKIWADENKYAKMLEVELAILEALEDRMVPKGTAAEIRARAQIRPERVDEIEKVTKHDIIAFCTSIAEQF
TAETGKFFHFGVTSSDIIDSALSLQIHDSMSYVIKDLEALCDSLLTKAEETKEIITMGRSHGMFAEPMSFGQKFLGAYVE
FKRRLKDLKDFQKDGLTVQFSGAVGNYCILTTEDEKKAADILGLPVEEVSTQVIPRDRIAKLISIHGLIASAIERLAVEI
RHLHRSDVFEVYEGFSKGQKGSSTMPHKKNPISTENLTGMARMLRSHVSIALENCVLWHERDISHSSAERFYLPDNFGIM
VYALRRMKNTIDNLVVQRDIIEDRVRSTSAYLSSFYLHFLVANTPFMREDCYKIVQQVAFDLKQGESFSKKLQKVMHDEH
NIILDIPEMDFEGIKKTYLKEIDHVFDRSVKA

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=408, Percent_Identity=22.5490196078431, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI183227688, Length=374, Percent_Identity=22.4598930481283, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1787376, Length=323, Percent_Identity=26.0061919504644, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17508577, Length=352, Percent_Identity=24.4318181818182, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32564234, Length=295, Percent_Identity=23.728813559322, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323391, Length=373, Percent_Identity=25.7372654155496, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24647570, Length=404, Percent_Identity=23.019801980198, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49371; Mature: 49371

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRYDIAKISKIWADENKYAKMLEVELAILEALEDRMVPKGTAAEIRARAQIRPERVDE
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHH
IEKVTKHDIIAFCTSIAEQFTAETGKFFHFGVTSSDIIDSALSLQIHDSMSYVIKDLEAL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHEEEHHHHHHHHHHHHHH
CDSLLTKAEETKEIITMGRSHGMFAEPMSFGQKFLGAYVEFKRRLKDLKDFQKDGLTVQF
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
SGAVGNYCILTTEDEKKAADILGLPVEEVSTQVIPRDRIAKLISIHGLIASAIERLAVEI
CCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RHLHRSDVFEVYEGFSKGQKGSSTMPHKKNPISTENLTGMARMLRSHVSIALENCVLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
RDISHSSAERFYLPDNFGIMVYALRRMKNTIDNLVVQRDIIEDRVRSTSAYLSSFYLHFL
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VANTPFMREDCYKIVQQVAFDLKQGESFSKKLQKVMHDEHNIILDIPEMDFEGIKKTYLK
HHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHH
EIDHVFDRSVKA
HHHHHHHHCCCC
>Mature Secondary Structure
MIKRYDIAKISKIWADENKYAKMLEVELAILEALEDRMVPKGTAAEIRARAQIRPERVDE
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHH
IEKVTKHDIIAFCTSIAEQFTAETGKFFHFGVTSSDIIDSALSLQIHDSMSYVIKDLEAL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHEEEHHHHHHHHHHHHHH
CDSLLTKAEETKEIITMGRSHGMFAEPMSFGQKFLGAYVEFKRRLKDLKDFQKDGLTVQF
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
SGAVGNYCILTTEDEKKAADILGLPVEEVSTQVIPRDRIAKLISIHGLIASAIERLAVEI
CCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RHLHRSDVFEVYEGFSKGQKGSSTMPHKKNPISTENLTGMARMLRSHVSIALENCVLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
RDISHSSAERFYLPDNFGIMVYALRRMKNTIDNLVVQRDIIEDRVRSTSAYLSSFYLHFL
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VANTPFMREDCYKIVQQVAFDLKQGESFSKKLQKVMHDEHNIILDIPEMDFEGIKKTYLK
HHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHH
EIDHVFDRSVKA
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571; 10673438 [H]