LOCUS NS_000194 5362 bp DNA circular ENV 16-DEC-2008 DEFINITION Uncultured Termite group 1 bacterium phylotype Rs-D17 plasmid pTGRD3 DNA, complete sequence. ACCESSION NS_000194 VERSION NS_000194.1 GI:189485779 KEYWORDS ENV; isolate genome. SOURCE uncultured Termite group 1 bacterium phylotype Rs-D17 ORGANISM uncultured Termite group 1 bacterium phylotype Rs-D17 Bacteria; Elusimicrobia; environmental samples. REFERENCE 1 AUTHORS Hongoh,Y., Sharma,V.K., Prakash,T., Noda,S., Taylor,T.D., Kudo,T., Sakaki,Y., Toyoda,A., Hattori,M. and Ohkuma,M. TITLE Complete genome of the uncultured Termite Group 1 bacteria in a single host protist cell JOURNAL Proc. Natl. Acad. Sci. U.S.A. 105 (14), 5555-5560 (2008) PUBMED 18391199 REFERENCE 2 (bases 1 to 5362) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (06-JUN-2008) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 5362) AUTHORS Toyoda,A., Hongoh,Y., Sharma,V.K., Hattori,M., Ohkuma,M. and Sakaki,Y. TITLE Direct Submission JOURNAL Submitted (01-OCT-2007) Contact:Yuichi Hongoh RIKEN, Ecomolecular Biorecycling Science Research Team; Hirosawa 2-1, Wako, Saitama 351-0198, Japan COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AP009513. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..5362 /organism="uncultured Termite group 1 bacterium phylotype Rs-D17" /mol_type="genomic DNA" /isolation_source="a single cell of termite (Reticulitermes speratus) gut protist" /host="Trichonympha agilis" /db_xref="taxon:471821" /environmental_sample /plasmid="pTGRD3" /note="rRNA phylotype Rs-D17" gene 1..1275 /locus_tag="TGRD_P3-1" /db_xref="GeneID:6372919" CDS 1..1275 /locus_tag="TGRD_P3-1" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_001956718.1" /db_xref="GI:189485780" /db_xref="GeneID:6372919" /translation="MWNEIKTELTEKIPTIWLEPVKEESFKDDILMLNVPNRYYAEKY KTDFKELIQSVIKTKIGKDIGLQCQIELLPEPITKDKKPKTTSKTPLIKETKFNGKPY FESPAELTEISKEISQAKEIDVDNTKLTSMPVKYSIKDVVSFSLLNSKMFTYPNDKRK KTKVEINIRFNNGTIKPLDLYRGQLDFNDEGYGQLTTTHAKIFLAITHIWQKQGCKFA NNSYLAVVDISIRELAKQLGYQKFSGADYKRLLRKTKELADFPMILADMYEAHTFTLL YDVSNHKLKKSRNNKNMLRILINPFIAKQLYERKVILRNPQCYKIKNPTAIKFLICYD KRIIKGNNLRLNIFEIANDLEVNINNITSVAENLKNAFQELNGYELNDSYSLHVELIK ENKEWIVVADRVLKEKQQSLKVNCRTDIECEA" gene 1470..1769 /locus_tag="TGRD_P3-2" /db_xref="GeneID:6372920" CDS 1470..1769 /locus_tag="TGRD_P3-2" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_001956719.1" /db_xref="GI:189485781" /db_xref="GeneID:6372920" /translation="MLSLKDNKKSFFSLMCLFFLVVCGCHSAKEFRDGYITGLADGYA LSVNEFKYNAELAKIKDEFDWSKVDFKSEMKMYLDENKDIEKKVYDDYVKNGRKV" gene 4523..4915 /locus_tag="TGRD_P3-3" /db_xref="GeneID:6372918" CDS 4523..4915 /locus_tag="TGRD_P3-3" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_001956720.1" /db_xref="GI:189485782" /db_xref="GeneID:6372918" /translation="MPTPTPPLTPASPEPASQDKLKKIALFCPPLTQLMFIYGLGLVE GAIQASLPYLFSSPTIISTLSIMFANMPIVGAMWFALFAYRSEVSTLSNVLDWSLPAL LVFGEFLYFCGVGAVSPFSHWLWSIDPV" ORIGIN 1 gtgtggaatg aaattaaaac ggaattaaca gaaaagatac cgactatatg gctagagcca 61 gtaaaagagg aaagctttaa agacgacata ttaatgctta atgtacctaa cagatattat 121 gctgaaaagt ataaaactga ttttaaagaa ttaattcaaa gcgtaataaa aacaaaaata 181 ggcaaagaca taggattaca gtgtcagatt gaattactgc cagaacctat cacaaaagac 241 aaaaagccaa agacaacatc aaagacgcct ttaataaaag aaacaaagtt caacggcaag 301 ccttattttg aaagtcctgc ggagcttaca gagatttcaa aagagataag tcaggcaaaa 361 gaaatagatg ttgataatac caaattgact tcaatgcctg ttaaatacag cataaaagac 421 gtcgtatctt tcagcctgct aaatagcaaa atgtttactt atccaaatga taaaagaaaa 481 aaaacaaaag ttgaaataaa tataagattt aataacggta ctattaagcc gcttgattta 541 tatcgaggtc agctagattt taatgacgaa ggatacgggc agttaacaac gacgcacgca 601 aagatattcc ttgccattac tcatatttgg cagaagcagg gctgtaagtt tgcaaacaat 661 agttatcttg ccgttgttga catctcaata agagaactgg ctaaacaatt aggctatcaa 721 aaattcagcg gagccgatta taaaagatta ttgcgaaaga ctaaagagct tgcagacttc 781 cctatgatac ttgccgatat gtatgaagct catacattta ctctcttata cgatgtttct 841 aatcacaaac ttaaaaaaag cagaaacaat aagaatatgt taagaatact tattaatcca 901 tttattgcga aacaacttta cgaaagaaaa gtaattttaa ggaacccgca atgctataag 961 attaaaaatc ctacagctat taaattcctg atatgttatg acaaaagaat aattaaaggg 1021 aacaatttga gactgaatat attcgaaata gcaaatgatt tagaagtaaa tataaataat 1081 ataaccagcg ttgcagaaaa tttaaaaaat gcattccaag aactgaacgg atacgaactt 1141 aacgatagtt atagcctgca cgtggaatta atcaaagaga acaaggaatg gattgtagtt 1201 gctgaccgtg tattaaaaga gaaacaacaa tcattaaaag tgaactgcag aactgacata 1261 gaatgcgaag cataagagga gcgaagctcc gtgcctgtcc ttgccttttc tttcttaaaa 1321 gcctgccttt aaaaaaagct caaatctaaa agtaaaaata gtcatatcaa acatcgtcta 1381 aaatctggtc tttatcttta aaaaaagttg ttaaaaaaga ttaaatgagg atatggttat 1441 aataaaatca aataaagagg attattaaaa tgttaagttt aaaagataat aaaaagagct 1501 tcttttcgtt aatgtgtctg ttttttttgg tcgtatgtgg atgccattct gcaaaagagt 1561 ttagagatgg gtatatcaca gggctagcgg atggatatgc cttgagcgta aatgaattta 1621 aatataacgc tgagcttgct aaaattaaag atgaatttga ttggagtaag gttgatttta 1681 aaagtgaaat gaaaatgtat ttagacgaaa ataaagatat agaaaaaaag gtttatgatg 1741 actatgtaaa aaacggcaga aaagtataaa gagtaaagta aagcaagggt agatttttta 1801 atgaaatctc attctctagc tctggttgta aatatggttt gaaataatga ggaacaaaaa 1861 gatgatgtta aataaatatc attattataa aaacgattaa aggaaaggcg ataactatga 1921 aaagccagtt aaatattacc tcaaaagaga aagataaaga acctttaaaa aattgctata 1981 aatgtgatgt aaaaagagta tatacggata acccgtatta tgctcccgtt gactatcagt 2041 tagacttgtt tgataataat aatatcttgc ctgttaaaag ttgtgtgcgt tgaaataaat 2101 aaacgtgcaa agcttaagtt tattgattat gcctcatata gaaaatatat atttaaaaat 2161 aaaatgttat aatagtgata ttaaataatt catattattt aacctccaaa attactctaa 2221 acggtaaagc ataaaggcaa gttgtttttg aataggacac ttgccttttt tgtttttgtt 2281 ggtatataat gtcagtattg cataatagct ttgtcctatt ctctgtcatg taatgccgaa 2341 aagtagttat aaggcgagtg tcaacctaag tcggagactg ttgaataatg caggcttctt 2401 gggctctggc ggataggctc taaagattgg tcaacgttcg taagagtaaa cggagtgtat 2461 taagagaaaa tatctttctc ttttcctttt ctctttctgc caaaaagtaa ttatgtaata 2521 ccttgccgtt gctttgtctt taataataaa agcaaaagta aaaatatcag ttgtaatata 2581 aactaatagg actgatatag aatgcgaagc ataaaaggag cgaagctcct tgcttgtttt 2641 ccctctctta aaagcctgcc tttaaaaaaa agctcaaatc taaaagtaaa aatagtcata 2701 tcaaacatcg tctaaaatct ggtctttaaa aaaaatgaaa aaaatctgtt gtcgttgacg 2761 ttctctggaa cacccttgag gctgtccagt agaagcagcg gtattcccgc ttatatgttg 2821 tctcgtcttt aaaatctatt ctgtttactt ctaaaaccaa aaaaaacgat taaaaatcgt 2881 aaaaactgcg tttaaggcac caacgtgcct taaataacac aaaacagacg ttcgctggca 2941 aagttccatt aagaacgtct taaaatgtca aaatatgcca atattgtgtc aaagtaaggc 3001 attatcacat agtctaaagc cttaaataaa ataaattaaa aaaataaaaa aggctaaatg 3061 actgtgataa gccttgaaaa gcttgtaaaa gctaaaaaaa acggatgtaa atccgtcaga 3121 cggactgtaa gttcgttaaa aagagctggc taaaactaaa gccagcgata aagcgtgcgt 3181 ataaagactt gattgtatta gcacgctgtc cacatcgttt ttgattgttg tgaaaaaatg 3241 tgaaaaaaaa tcttatgttt taagatgtaa aagatatgtt tctcctgcca tttacttgtc 3301 atgcaatttg cctactctga catatttgct cttttctctc ttaaaagtcc gtctttaaaa 3361 agctcaagtc taaaagtaaa aatagtcata tcaaacgttg tctaaaatct tgtctttaaa 3421 aaaaataatg aaaaagaatc tcttgtcgtt ggcattataa accttacttg atgtatataa 3481 tgcccattga cgataaattc gaggcaaaaa atgccaattg tatatacttg atatacaatt 3541 gggaggttgt gtctttaata tccaattggc tagctgtact caaaataata acgatagttt 3601 atgaaagcgt aaaaagctaa gacattatga taatgcttag tcgctgtttg taaatatgtc 3661 taaaaattta ttatatattt ataggcttgc acgacattta gataacgtct gtgatgtata 3721 aatagtcaaa agccgtctta aagtagacgg caaaaaaaaa acagtaaaag cgaaggaagt 3781 gagcgaccct cttaacttga tatactatat gctagatggt gccgaaaagt taatacatat 3841 cttgtcattg ccttgtcttt tctctcttaa aatttctgtc tttaaaaaag ctatgatgaa 3901 atattaatta gcctaaggat attaagtgaa atatagaaaa gaatttgatt gctgtggaaa 3961 cgatatgcgt catactgctg acgcaccaaa acttgaaaag agattgaaaa aatctcttaa 4021 cgtttttgac ttggtactgt tgactgcgta tctgtttggt ttgttgttat tgacatcttg 4081 ttctcgtgtt aatacgttcg gttataagat ctatggtagg caagagctgc tcgacgaagt 4141 ctgtagatta aaacatattg taatgaaatt agaaggagag gggaaagtta gtagtacgat 4201 tgttctctag actgaaagat attcttatga aaaagcttat agaaaatgta tttcctatta 4261 acaatccgtt tagaagttct agaactgttc aaaaagcttt aaaaataatt aataaaaaag 4321 ttgttaaaaa agattaaatg aggatatgac tgtaatgaaa tttaaaaaaa tatttgctgt 4381 gattttaatg ttgtctgtgt tcttgtctgg ctgtcatagg aaggttataa caggcatttc 4441 taaaaatgaa aatgatgttt caacttcaac gaatactcct gcttcagagc ctactcctac 4501 tccgacgcct acttctacgc caatgcctac gcctactcca ccgcttactc ctgcttcacc 4561 agagcctgcg tctcaagaca aactcaaaaa aatagcgttg ttttgcccgc cattaacgca 4621 attaatgttt atttacggct taggcctagt tgaaggtgca atacaagcat cattgccata 4681 tctcttttct tcaccaacaa tcatatcgac tttaagcata atgtttgcaa atatgccaat 4741 tgtcggcgca atgtggttcg cactattcgc ttataggagc gaagtatcga ccctgtctaa 4801 tgtacttgat tggagtttgc cggctttact tgtgtttgga gaatttctgt atttctgtgg 4861 agtaggagca gtcagtcctt tcagtcattg gctatggtca atcgaccctg tctaatgtac 4921 ttgattggag tttgctgttg aaatatacgc ttatttagaa ttctacgtta aaaactcgat 4981 taattattaa attataaatt atgaaaaagc ctgcagaaat gcaggctttt tttgtgttgg 5041 attatatgtt gactatgtgt tagtgataaa ttataatata tgtgttggtg ataaatttaa 5101 ttaacaaaat atgtgttttc ggtaaagaga ttatgtgttt tcggtaaaga gattatgtgt 5161 tttcggtaaa gagattatgt gttttcggta aagagattat gtgttttcgg taaacgattt 5221 tagcaaaaaa gtggctataa ataaggattt ggcttactct ctaactctaa taagacctaa 5281 taagactaag tctgcctaaa agctggcaga cattttttgg ggaacttaaa agataaaaag 5341 acaaaaacaa aaggaataaa aa //