LOCUS       NC_015664              12355 bp    DNA     circular BCT 16-JUN-2011
DEFINITION  Frankia symbiont of Datisca glomerata plasmid pFSYMDG01, complete
            sequence.
ACCESSION   NC_015664
VERSION     NC_015664.1  GI:336176124
DBLINK      Project: 46257
KEYWORDS    .
SOURCE      Frankia symbiont of Datisca glomerata
  ORGANISM  Frankia symbiont of Datisca glomerata
            Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
            Frankineae; Frankiaceae; Frankia.
REFERENCE   1  (bases 1 to 12355)
  AUTHORS   Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S.,
            Peters,L., Mikhailova,N., Chertkov,O., Teshima,H., Han,C.,
            Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I.,
            Berry,A., Pawlowski,K., Persson,T., Vanden Heuvel,B., Benson,D. and
            Woyke,T.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of plasmid 1 of Frankia symbiont of Datisca
            glomerata
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12355)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2011) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 12355)
  AUTHORS   Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S.,
            Peters,L., Mikhailova,N., Chertkov,O., Teshima,H., Han,C.,
            Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I.,
            Berry,A., Pawlowski,K., Persson,T., Vanden Heuvel,B., Benson,D. and
            Woyke,T.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2011) US DOE Joint Genome Institute, 2800
            Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to CP002802.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4085684
            Source DNA and organism available from David Benson
            (david.benson@uconn.edu)
            Contacts: David Benson (david.benson@uconn.edu)
                      Tanja Woyke (microbe@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            
            ##MIGS-Data-START##
            investigation_type     :: bacteria_archaea
            project_name           :: Frankia symbiont of Datisca glomerata
            collection_date        :: Missing
            lat_lon                :: Missing
            depth                  :: Missing
            alt_elev               :: Missing
            country                :: Missing
            environment            :: Host, Plant symbiont, Soil
            num_replicons          :: 3
            ref_biomaterial        :: Missing
            biotic_relationship    :: Symbiotic
            trophic_level          :: Chemoorganotroph
            rel_to_oxygen          :: Aerobe
            isol_growth_condt      :: Missing
            sequencing_meth        :: WGS
            assembly               :: Newbler v. 2.3 (pre-release)
            finishing_strategy     :: Finished
            GOLD Stamp ID          :: Gi04474
            Funding Program        :: DOE-CSP 2007
            Isolation Site         :: Datisca glomerata
            Host Name              :: Datisca glomerata
            Cell Shape             :: Filament-shaped
            Motility               :: Nonmotile
            Sporulation            :: Sporulating
            Temperature Range      :: Mesophile
            Gram Staining          :: Gram+
            Symbiotic Relationship :: Mutualistic
            Diseases               :: None
            Phenotypes             :: Non-Pathogen
            ##MIGS-Data-END##
            
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: Finished
            Current Finishing Status :: Finished
            Assembly Method          :: Newbler v. 2.3
            Genome Coverage          :: 30x
            Sequencing Technology    :: 454/Illumina
            ##Genome-Assembly-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12355
                     /organism="Frankia symbiont of Datisca glomerata"
                     /mol_type="genomic DNA"
                     /strain="4085684"
                     /host="Datisca glomerata"
                     /db_xref="taxon:656024"
                     /plasmid="pFSYMDG01"
     gene            375..4004
                     /locus_tag="FsymDg_4549"
                     /db_xref="GeneID:10785547"
     CDS             375..4004
                     /locus_tag="FsymDg_4549"
                     /inference="similar to AA sequence:KEGG:Xcel_3411"
                     /note="KEGG: xce:Xcel_3411 hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_004595131.1"
                     /db_xref="GI:336176125"
                     /db_xref="GeneID:10785547"
                     /translation="MTAPAEDPRLLAAMDGAPDPVEPPADDDSPTSPRTTSRKVGAGR
                     SGESKSVAGRTRPHLANVSHDAPPVGGRHTLNGRTYFQRETGLFAVNKEQEEYQIAPG
                     TMEVTGKTELRDVDRERAPGKIVETLFHTRYTPPGAEPREVEVTDRDMRTREPQWPHA
                     LGIGGLIGARGPQAVGEAVRAMGFTVQMRGELRQAYAVQGPLRRPSGEWVYTRYGAPA
                     LTRDGVDDTVYAMLHPDIAEAEAFRVLGIGEPSTDETYADDVAVMLLFLSLHDDPAIG
                     WVHLAQLGYGPWAGLSGLRHLATAIVGTTGSRKSAVSGLINAAQSRGFMPTRARAHIP
                     TVKMRHAASSKVGAERTLYHYGSGTATVDDLFPGNQTASEVRADWITASAIADKMADQ
                     SGGQKGRREGNGLNPGLYPRCCLHFTMEELPYVDQHGSEAARLLIVWMDGPVNIDILS
                     FLQEPQNVTAVRRAYARMIRDVLADIDMPIRAHARALDTMDRWPRVGHNRAWDNGAAM
                     LTGALLFTDAVERSGFGPTGHLVAQAEATVREILARQASRCGMVGDRQAARDYVTLFV
                     KGVRKLLKAGSMHLSGPMRDATGAAIPPHVPDYHSDDFGWIATPIRPSDDSTEAGSLW
                     RASGKAFGAIHRRVEGATGRVQNDFPTGSWRVRVAEWPELCELIAGTTDWPMPHASEL
                     RRILAKDGLLHSEKAWVAAIWDGRREKTLSLDLHRIMAWTDDDDDAPNNSGGRDQDGD
                     DSGGGDDTPTPDPALAELASAEQHGMSYPSALPLDALGLTPEQAAMAANGEVSTGPCL
                     GCGEPMAPHVPGDGVYVDATGAYVQAHPSCEPPAPAAPAPAAPAPAAPAPAAPAPAAW
                     TAPELTPAGLESLRRTLAEVEWEGPTDDTFIGDVAARIGSAFPSKGAPGSGGPGLVWG
                     EGRGLTGYRLFRMVQGRAPRRLPPDERIGDISHPVPTPRATWYRPLTPEERAARFLVR
                     YDVNGQYLAAAGAIPLGTGRPIHLAERVIISPKAPAYYRLTVAPEAGDVAPFVAHGDD
                     WYSAPGARYLADRGLVRSVDEGLVWTDGHPWLDRWYRATRDARAYLATGTASVDLAAL
                     AAVKAMYATFLGGWLASEQYNATPLFRPDWRAHVRDRAAGNQARSLDKVRETSGRTPF
                     ALFKDAAYFTASSPDDIPGGMVVSGQLGKWKREAYGELAPDIIEILNSGADDVFGALR
                     KAVGR"
     gene            4001..4519
                     /locus_tag="FsymDg_4550"
                     /db_xref="GeneID:10785537"
     CDS             4001..4519
                     /locus_tag="FsymDg_4550"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_004595132.1"
                     /db_xref="GI:336176126"
                     /db_xref="GeneID:10785537"
                     /translation="MTPEEEMQKFADSFDWRDGFRSLVEEGKSNREIVEALDLTNGGA
                     IKRASAMRTAQRYRKLVTGGSGQTRGGRKGPQDTRVLEALKRRAAADKLRKPMVIGWK
                     AAVNVKSNSKGAGTRGQDLAPFGGANAAADALERGDYGEAGGAFSRALMQQYGAESFL
                     EVKSFERLDLQY"
     gene            complement(5760..5969)
                     /locus_tag="FsymDg_4551"
                     /db_xref="GeneID:10785546"
     CDS             complement(5760..5969)
                     /locus_tag="FsymDg_4551"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_004595133.1"
                     /db_xref="GI:336176127"
                     /db_xref="GeneID:10785546"
                     /translation="MKIPGFSTRADGKTRSNLISNPATGQDRSAVKAVRRALGAVTEC
                     PTCGGVGSIGKGGHCADDWHKNCGH"
     gene            complement(6039..6419)
                     /locus_tag="FsymDg_4552"
                     /db_xref="GeneID:10785538"
     CDS             complement(6039..6419)
                     /locus_tag="FsymDg_4552"
                     /inference="similar to AA sequence:KEGG:Strop_0070"
                     /note="KEGG: stp:Strop_0070 hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_004595134.1"
                     /db_xref="GI:336176128"
                     /db_xref="GeneID:10785538"
                     /translation="MIRFLDPDGTRYGIPTWPWGMAPSGLYTRTQLREIGFRPTSPGG
                     PAGQLMWRSQRADAGGIRTALLYPIGQTVQRTTATPRQMAALDRAHAARKTCPDCHRN
                     VGYTIPTHLGACLDCTSPQAKNAA"
     gene            complement(6700..8679)
                     /locus_tag="FsymDg_4553"
                     /db_xref="GeneID:10785539"
     CDS             complement(6700..8679)
                     /locus_tag="FsymDg_4553"
                     /inference="similar to AA sequence:KEGG:ML5_1658"
                     /note="KEGG: mil:ML5_1658 hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_004595135.1"
                     /db_xref="GI:336176129"
                     /db_xref="GeneID:10785539"
                     /translation="MARHDLSTRTPARIVGWRWVSGSPLDGVPRTDATWLHPATTVIH
                     PSGRAYRWQHRPRWQRAAHRTGGTLAVLGEAYGQIADPTLTTSLTAVAVAGGLAYAGN
                     RAHRRWVHRQHRRRLVQPLMKSLSGPLQLPIGASADKWLIVPRNPAKQGSITRVNLPD
                     HWVGEEYQQLAVHRLIDRWSKAPMEVSRVHLTSAPRFMEFMPTPQPPDAVEWESSDDP
                     YVMHIGAGPGKAPVYARTETDVPHLAVVAGSGSGKTTTLTVPLLHNREHGALVDIIDL
                     KRMSFTERKPEHPDGIAGDPTRPSRTVSGVRIHTTIEGAIRALAEFIASATAVALMQQ
                     EGYSTEDIPARVLIIDEFGSFAGGAKQWWAGPGKQKKQSPVPFWLYVTLMQGRALEHR
                     VVLGAHQLALKLFGDTGSDARDLFSGRILTGTCSAQKWITTYGHVKKPAWDASIKGRG
                     TYGPLGERPDLFQVAYVHPASANARLRALPEAPEWFDAGRCAPWITEEHIREVESSYG
                     AGRWVREWEHLFAEDDEEGQEEDAQPAASGSLPAAEGWVPDVTVGGWTPDVTDYVTVT
                     HADSTGDPSTDDDRDTPAAVTVQPVTADTDGDGTAERITLPDACRTGVIPLKPGTARA
                     ARMRDPKFPKGQGEGRDQTYTPEELQAWYANRIAQ"
     misc_feature    complement(<7849..7959)
                     /locus_tag="FsymDg_4553"
                     /note="AAA-like domain; Region: AAA_10; pfam12846"
                     /db_xref="CDD:193320"
     gene            complement(8792..9229)
                     /locus_tag="FsymDg_4554"
                     /db_xref="GeneID:10785540"
     CDS             complement(8792..9229)
                     /locus_tag="FsymDg_4554"
                     /inference="protein motif:TFAM:TIGR00621"
                     /note="KEGG: amd:AMED_0360 single-strand DNA-binding
                     protein;
                     TIGRFAM: Single-strand DNA-binding;
                     PFAM: Primosome PriB/single-strand DNA-binding"
                     /codon_start=1
                     /transl_table=11
                     /product="single-strand binding protein"
                     /protein_id="YP_004595136.1"
                     /db_xref="GI:336176130"
                     /db_xref="GO:0003697"
                     /db_xref="InterPro:IPR000424"
                     /db_xref="InterPro:IPR011344"
                     /db_xref="GeneID:10785540"
                     /translation="MQEAVITIAGNLANDPELRFTPNGAAVASFVVACTERRRDQNTG
                     EWSDGATSYHRCTAWRQTAENAAESLRKGDRVLVVGTLRQSSWEDKQSGETRYGWDVQ
                     VDEVAVSVKFHAARSMRPERSGAADGTSTTTPGRGTAVPAHQS"
     misc_feature    complement(8912..9214)
                     /locus_tag="FsymDg_4554"
                     /note="SSB_OBF: A subfamily of OB folds similar to the OB
                     fold of ssDNA-binding protein (SSB). SSBs bind with high
                     affinity to ssDNA. They bind to and protect ssDNA
                     intermediates during DNA metabolic pathways. All bacterial
                     and eukaryotic SSBs studied to date...; Region: SSB_OBF;
                     cd04496"
                     /db_xref="CDD:72968"
     misc_feature    complement(order(8933..8935,8942..8944,8978..8980,
                     9005..9007,9068..9070,9074..9076,9122..9130,9203..9214))
                     /locus_tag="FsymDg_4554"
                     /note="dimer interface [polypeptide binding]; other site"
                     /db_xref="CDD:72968"
     misc_feature    complement(order(8924..8926,8930..8932,8972..8977,
                     8981..8983,8987..8989,9011..9016,9053..9055,9059..9061,
                     9065..9067,9071..9073,9077..9082,9119..9124,9143..9145,
                     9173..9175,9191..9199))
                     /locus_tag="FsymDg_4554"
                     /note="ssDNA binding site [nucleotide binding]; other
                     site"
                     /db_xref="CDD:72968"
     misc_feature    complement(order(8993..8995,8999..9001,9005..9007))
                     /locus_tag="FsymDg_4554"
                     /note="tetramer (dimer of dimers) interface [polypeptide
                     binding]; other site"
                     /db_xref="CDD:72968"
     gene            complement(9279..9626)
                     /locus_tag="FsymDg_4555"
                     /db_xref="GeneID:10785541"
     CDS             complement(9279..9626)
                     /locus_tag="FsymDg_4555"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_004595137.1"
                     /db_xref="GI:336176131"
                     /db_xref="GeneID:10785541"
                     /translation="MAAVERVTDPIGHPLPEHPPPLHPEGNCPVTILAVLVLLLAGIH
                     LAERAAHLARAVLATLRRPVVALSVALVLIYGRPVLAHPEVVTGALVAILAALAGVAG
                     LVHLARTRVPRVA"
     gene            complement(9641..10609)
                     /locus_tag="FsymDg_4556"
                     /db_xref="GeneID:10785542"
     CDS             complement(9641..10609)
                     /locus_tag="FsymDg_4556"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_004595138.1"
                     /db_xref="GI:336176132"
                     /db_xref="GeneID:10785542"
                     /translation="MKFRVETQKYTDTHTVNGESAPVTRTRRVAVPVLPRDIDVIAVR
                     GVVGLVLALTLVSITWSTVSIAHLLNGTWTAYLAGAVFDLAWLATLGMSFIVRFRPDR
                     RATVDKIGWALLAVTVTAIALEGLQTGGVAMACIGASVSIAAKVLWWTLNRATAPDLS
                     PDDAQWVAQQISRASAQMAVAAVRRQVARVEDQATAQMLAMEASRRPPIGTDVVGLPV
                     GEVATVADSPTPPVGRQVDVLTEVRQVTALSAPMVEDTPDVPKDNGTPRGSKARRVRE
                     LVASGVTDVPTIRERLADSGVTVDPSHVRRIIRESRAAANEPNPYL"
     gene            complement(10606..10866)
                     /locus_tag="FsymDg_4557"
                     /db_xref="GeneID:10785543"
     CDS             complement(10606..10866)
                     /locus_tag="FsymDg_4557"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_004595139.1"
                     /db_xref="GI:336176133"
                     /db_xref="GeneID:10785543"
                     /translation="MTNLRPPDHCEGCGGELDLTPAPSGCDYCIPASTPDGRFHHMPV
                     SPDSADLDWLRGMVRDMGRALDARDFVRLAALVTEARGEVTR"
     gene            complement(10942..11151)
                     /locus_tag="FsymDg_4558"
                     /db_xref="GeneID:10785544"
     CDS             complement(10942..11151)
                     /locus_tag="FsymDg_4558"
                     /inference="ab initio prediction:Prodigal:1.4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_004595140.1"
                     /db_xref="GI:336176134"
                     /db_xref="GeneID:10785544"
                     /translation="MTVQTEIPRERVSYYLSKPIIDAVERLTLELSLELGKRVTKADV
                     VDGLLTLGLDQRAKLVREIRKSKGL"
     gene            complement(11457..12164)
                     /locus_tag="FsymDg_4559"
                     /db_xref="GeneID:10785545"
     CDS             complement(11457..12164)
                     /locus_tag="FsymDg_4559"
                     /inference="protein motif:PFAM:PF01656"
                     /note="PFAM: Cobyrinic acid a,c-diamide synthase;
                     KEGG: gvi:glr2463 hypothetical protein"
                     /codon_start=1
                     /transl_table=11
                     /product="Cobyrinic acid ac-diamide synthase"
                     /protein_id="YP_004595141.1"
                     /db_xref="GI:336176135"
                     /db_xref="GO:0042242"
                     /db_xref="InterPro:IPR002586"
                     /db_xref="GeneID:10785545"
                     /translation="MCDIRLWMFTTQGVARAMRILIFASQKGGVGKTTSALNITHLLA
                     ETLAARVLLLDTDPNGDSARWADRARAAGVQLRFDYTAETDPRLLGHVREVAEYDVIV
                     VDTPGARESAALLAVVRHADAVILPSPPAALDLAGLARTVRDVVRPSGVPFRVLLTRV
                     DPRSIPRAEQARTDLVEQGYGVFSTIIRSYAAHEDAALAGVPIGAYRGRYASEADLDY
                     RRVTAEVIRDYVTGDIR"
     misc_feature    complement(11484..12116)
                     /locus_tag="FsymDg_4559"
                     /note="ATPases involved in chromosome partitioning [Cell
                     division and chromosome partitioning]; Region: Soj;
                     COG1192"
                     /db_xref="CDD:31385"
     misc_feature    complement(11691..12107)
                     /locus_tag="FsymDg_4559"
                     /note="ParA and ParB of Caulobacter crescentus belong to a
                     conserved family of bacterial proteins implicated in
                     chromosome segregation. ParB binds to DNA sequences
                     adjacent to the origin of replication and localizes to
                     opposite cell poles shortly following...; Region: ParA;
                     cd02042"
                     /db_xref="CDD:73302"
     misc_feature    complement(12066..12086)
                     /locus_tag="FsymDg_4559"
                     /note="P-loop; other site"
                     /db_xref="CDD:73302"
     misc_feature    complement(order(11853..11855,12066..12068))
                     /locus_tag="FsymDg_4559"
                     /note="Magnesium ion binding site [ion binding]; other
                     site"
                     /db_xref="CDD:73302"
ORIGIN      
        1 gcttgatgtg gcggcggacg cttcatcgtg tctcgtgatc cgtgccgctc tcgcgtacgc
       61 ggccggcgtc gacgtcgcgg actacacccc ccgacgcaca ggccgtccgc agggcgccaa
      121 ggacaggcga ccccgaaccc gtcagcaggc gggggacgga gagcgggcgg cgtgatgccc
      181 tgaaacgcaa gcggcccggg tgctggcaac acccgggccg gacacccccg aaccccttca
      241 cgagaactgg agtgctacgt gcacaccaat cgtacggtcg cgcgcgcgtc tgcgcctgac
      301 acccggacgg agtcccggga aactcaccac gacacccacc ccgttcgggg taccagctcc
      361 ggcgaggtgg gccagtgacg gcccccgcgg aggacccgcg tctcctcgcc gcgatggacg
      421 gtgcgccgga ccctgtcgag ccacccgcgg acgatgacag ccccacctcg ccacgcacga
      481 cgtcacgcaa ggtcggagcg ggccggagcg gcgagagcaa gagcgtcgcc ggtcgcaccc
      541 gtccccacct cgcgaacgtc tcccacgatg ccccaccggt cggcggacgc cacaccctga
      601 acggtcgcac ctactttcag cgcgaaacgg gcctgttcgc ggtgaacaaa gagcaggagg
      661 aataccagat tgcccccggg actatggagg tcacgggaaa aacagaactc cgtgatgtcg
      721 accgggaacg tgcccccgga aagatcgtag agacgctctt ccatacgcgg tacacaccgc
      781 ccggggctga gccgcgggaa gtcgaggtaa ccgaccggga catgcgtaca cgcgagccac
      841 agtggccgca cgcactcggt atcggcggac tcattggagc ccggggcccg caagcggtcg
      901 gagaagccgt ccgggccatg gggttcacgg tccagatgcg aggcgaactc cggcaggcgt
      961 acgccgttca ggggccgcta cgccgtccat cgggcgagtg ggtctacacc cggtatggcg
     1021 caccggcact caccagagac ggtgtcgatg acacggtgta cgcgatgctg cacccggaca
     1081 ttgcggaagc agaggctttc cgtgtgctcg gcatcgggga accgtcaacc gatgagacct
     1141 atgccgatga cgtggccgtg atgttgctgt tcctgtcgct gcatgacgat ccggctatcg
     1201 ggtgggtgca cctcgcccag ctcggttacg gcccgtgggc ggggctctct ggcctgcgtc
     1261 acctcgccac agcaatcgtc ggaacaaccg gatcacggaa aagcgccgtg tccgggttga
     1321 taaacgcggc gcagtcccgg ggcttcatgc ccacccgcgc ccgtgcgcac atcccgacgg
     1381 tgaagatgcg gcacgccgcg tcgtcgaagg taggcgcgga acgtacgctc taccactacg
     1441 gctccggtac cgcgacggtc gacgacctgt tcccaggaaa ccaaaccgcc tcggaagtcc
     1501 gagcagactg gattaccgct tcggctatcg cggacaagat ggctgaccag tccggcggac
     1561 agaaggggcg ccgcgagggt aacgggctga accctggcct atatccacga tgctgcctcc
     1621 atttcacgat ggaggaactc ccttacgtgg atcagcacgg ttcagaagcg gcacggctcc
     1681 tcattgtttg gatggatggg cccgtcaata tcgacatcct gtcgttccta caggagccgc
     1741 agaacgtcac tgcggtccgg cgcgcctacg cgcgaatgat ccgcgacgtc ctggccgaca
     1801 tcgacatgcc gatacgggca cacgcgcgcg cactcgacac gatggacagg tggccacggg
     1861 taggccacaa cagagcatgg gacaacggcg cggcgatgct gaccggcgcg ctcctgttca
     1921 cagacgctgt cgagcgttcc gggttcggcc cgaccgggca ccttgtggcg caggccgaag
     1981 cgacggtgcg ggagatcctg gcgcgtcagg cgtcccgatg cgggatggtg ggagaccggc
     2041 aggccgcacg cgactatgtg actctgttcg tgaaaggtgt tcgaaagctt ctgaaagccg
     2101 gttcgatgca cctttccggt ccgatgcggg atgcgacagg tgcggctatc ccgccgcatg
     2161 ttcccgacta tcactcggac gattttgggt ggatagctac gccgatacgt ccgagcgatg
     2221 acagcaccga agctgggtcc ctgtggcgag cgtcgggtaa ggcgttcggg gcaattcacc
     2281 ggcgcgtgga aggtgccacg ggtcgagttc aaaacgattt ccccaccggc tcctggcggg
     2341 ttcgggttgc cgagtggccg gaactctgcg aactcatcgc aggcacgacc gactggccga
     2401 tgcctcacgc ttccgaactc cggcggattc tcgcgaaaga cggcctactc cactccgaaa
     2461 aagcatgggt ggctgcgatc tgggacggga ggcgggagaa gacgctgtct ctcgaccttc
     2521 accgaatcat ggcgtggacc gacgatgacg acgacgcgcc caacaacagt ggaggtcgtg
     2581 accaagacgg tgatgactcc ggcggtggcg atgacactcc gacgccggac ccagctttgg
     2641 ccgaactcgc gtcggcggag cagcacggga tgagctaccc gtccgccctt cccttggacg
     2701 cactcggact caccccggaa caggccgcga tggccgcgaa tggggaggtg tcgaccggcc
     2761 catgcctcgg gtgcggtgag ccgatggcac cccacgtccc gggagacggc gtctacgtcg
     2821 atgccactgg cgcctacgtc caagcgcatc cgtcatgcga gccaccagct ccggccgcac
     2881 cagctccggc cgcaccagct ccggccgcac cagctccggc cgcaccagct ccggccgctt
     2941 ggacggcacc ggagctgacg ccggccggct tggagtcgct ccgccggaca ctcgcggaag
     3001 tcgagtggga gggtccgacc gatgacacgt tcatcggcga cgtggccgcg cgaatcgggt
     3061 ccgcgttccc gagcaagggt gcacccggct ccggcggccc gggtctcgtc tggggcgagg
     3121 ggcgggggct caccggctac cgcctgttcc ggatggtgca agggcgtgca cctcggcggc
     3181 ttcccccgga tgagcggatc ggggacatct cccaccctgt tccgaccccc cgggcgacgt
     3241 ggtacaggcc gctcaccccg gaagagcggg ccgcacggtt ccttgtcagg tacgacgtca
     3301 acgggcagta cctcgctgcc gcgggagcga tcccgctcgg caccggaagg ccgatccacc
     3361 tcgcggagcg ggtcatcatc tcgccgaaag ctccggcgta ctaccggctg accgtcgcac
     3421 ccgaagcggg cgacgtggcg ccgttcgtgg cacacgggga tgactggtac tccgccccgg
     3481 gcgctcgata cctcgctgat cgcggactcg tccgaagcgt cgatgagggg ctggtctgga
     3541 cggacgggca cccgtggttg gacaggtggt accgggccac acgtgacgcg cgggcgtacc
     3601 tcgccaccgg aaccgcgagc gtcgacctgg cggcactggc cgcggtaaag gcgatgtacg
     3661 ccacgttcct cggggggtgg ctcgcgtcgg agcagtacaa cgcgacaccg ctgttccggc
     3721 cggattggcg cgcacacgtt cgggaccgcg ccgcggggaa tcaggcacgc tcgctcgaca
     3781 aggtccggga gacatccggg cggaccccat tcgcgctatt caaggatgcg gcgtatttca
     3841 cggcttcatc gccggacgac atcccgggtg ggatggtggt gtccggccag ttgggcaagt
     3901 ggaagcgcga agcgtacggc gaactcgccc ccgacataat cgagatcctg aactccggcg
     3961 cggacgacgt attcggcgca ctgcgtaagg cggtagggcg atgactcccg aagaggaaat
     4021 gcagaagttc gcggactcgt tcgactggcg cgacgggttc cgttccctcg tggaagaggg
     4081 gaagtcgaat cgcgaaatcg tcgaagcgct cgacttgacc aacggaggag ccatcaagcg
     4141 cgcgtcagca atgcgaacgg cacagcgata ccggaagctc gtcacgggag gtagcggcca
     4201 aacccgcggc gggcgcaagg ggccgcagga tactcgcgtg ctggaagcgt tgaagcggcg
     4261 cgcggcagcg gacaagttgc gtaagccgat ggtcatcggc tggaaagcgg ctgtgaacgt
     4321 caagagcaac agcaaggggg ccggtacccg ggggcaggat ctcgcaccct tcgggggtgc
     4381 gaatgctgcc gccgatgccc ttgagcgtgg cgactacggg gaagccgggg gtgcgttcag
     4441 ccgtgcgctg atgcagcagt acggggccga aagcttcctt gaggtgaaga gcttcgagcg
     4501 gctcgacctc cagtactgac agcacacgcg agaccccccg gcgatcacgg accgccgggg
     4561 ggtctcgcgt gtgtcccctc ccctccgccg cgcaccctcg acggccgcgc accctctgtt
     4621 aatacgactc actatagggc tggccccctg cgggagcatt agtgcggcgc tgcgcttgca
     4681 cggggccagc attcttgagc gcagcgaaag aattcccccc gacattccgg cgccattcat
     4741 gctgagcatt catggcacat tctttgggtg attgtttgac gtttccagcg cgcactcatg
     4801 gcgcctattg tttggcgttt ccttcggcgc gcattgatgg acatccgact gtggacgtcc
     4861 gactatcgac atccgtcttt ctacatctga cttcggatgt tcggatggcg gactgcgccg
     4921 agggtgccgg ccgcgccgag ggtgccggcc gcgccgaggg tgccggccgc gccgagggtg
     4981 cccatgtgcc acgtaggcgg cggatgacgt ccagttggat agatgacacc ggagagtgga
     5041 gatcttgcca ctacgggccg cgtgggtacc gtagcggcga ttcggggtgg tcgagctgtg
     5101 gcgcgctggg gtgtcggtca ctctccgtcg atccgcgcgc ggcctgagcc ccctcacgct
     5161 ggggagaatt tccgcgcgca ctcacatgcg gttcggaacg ttcggaaggt tcggaacgct
     5221 ggtgtgcaag gtgatcgcaa ggttcggaac gggtctggag aggttcggaa cgggttcgga
     5281 acgggttcgg aacgggttcg ggccccgctc cggccgcttc ggcgccgttt gccacctcgc
     5341 gcgctagtca ctctccgtgg ttcacggtgg ccacctcgcc tgcgagtccg ggccacctcg
     5401 ccgtggtcct ggccgtcgac gtgcccgacg ccgggccgaa ggtccggtgg cggtagtccg
     5461 ggcaggccgg tgaggtggcg gagctggtgt ccatggacat gtccacatcg gtgccggtgg
     5521 tggcgtatcc cccggcgtag caggcgttcc ggggtggatg tccatggaca tgtccacgtg
     5581 gacatcgtcg gccgtcgagg gtgcgcggcc gtcgagggtg cgcggccgtc gagggtgcgc
     5641 ggccgtcgag ggtgcgcggc cgtcgagggt gcgtggccgt cgagggtgcg cggccccgga
     5701 cgcagaacac ccccgactgg agaggaaacc agccggggtg ttgggtctcg cgggctgact
     5761 cagtggccgc agttcttgtg ccagtcatcc gcgcagtggc cgcccttacc gatggagcca
     5821 acgccaccgc aggtggggca ctccgtgacc gcgccgagcg cgcggcgaac ggccttcact
     5881 gcggacctgt cctgtcccgt cgcagggttg gagatgaggt tcgaccgggt cttaccgtcc
     5941 gcgcgagttg agaagccggg gatcttcatg ttttcctcct tgagcgaatg cggttttgcg
     6001 ggttccgggg ctgctgtgat cttatcttgt tctactgctc aggcagcatt ttttgcctga
     6061 ggcgatgtgc agtcaaggca ggctccgaga tgggttggga tcgtgtatcc gacgttccgg
     6121 tgacagtcgg ggcacgtctt ccgtgcggcg tgcgcgcggt cgagtgcggc catctgtcga
     6181 ggggttgcgg tggtccgctg gaccgtctgg ccgatcggat agagaagcgc ggtccgaatc
     6241 ccgccagcgt ccgcgcgttg ggagcgccac atcagttgtc cggcagggcc ccccggagag
     6301 gtgggccgga atccgatttc ccggagctga gtccgggtgt agagccccga tggcgccatg
     6361 ccccacggcc aggtgggaat gccgtatcgg gttccgtccg ggtccaggaa tcggatcacg
     6421 gggtgccctc ccgggtctgt cacaggaatt gggttcgcgc ttccggctgt gtcgcatctg
     6481 atgtgcgtcg ccgtgtccgg ggttggttcc cggcgttgag ctgatcgtgc atgtccgggc
     6541 gctgttcggc gcatgtcttg tggcaggggg agccgttcgg cgcccggacg attgcgggac
     6601 ggtggcagat gacgcacggt gtgggttttc cgatgccgcc tttcggggaa ccgctgttgc
     6661 cggaccaatc gagcggaacg gggtcagggt tccgctcgac tattgggcga tccggttcgc
     6721 gtaccacgcc tgaagctctt ccggcgtgta ggtctggtct cgcccttctc cctgcccctt
     6781 tgggaatttc gggtcacgca ttctcgccgc gcgggccgtt cccggcttga gcgggatcac
     6841 gcctgtccgg cacgcatcgg ggagggtgat ccgctccgcc gtgccgtcac cgtcggtgtc
     6901 cgctgtcacg ggctggaccg tgacagcggc cggggtgtca cggtcatcgt cggtggacgg
     6961 gtcccctgtc gagtccgcgt gcgtgaccgt cacgtagtcc gttacgtccg gggtccagcc
     7021 acccacggtg acgtccggca cccacccctc tgccgcggga agcgagccac tcgcggccgg
     7081 ctgtgcgtct tcctcctgcc cttcttcgtc gtcttcggcg aacaggtgct cccactcgcg
     7141 gacccaccgg ccggcaccgt aggagctttc gacttcccga atgtgctcct ccgtaatcca
     7201 cggggcgcac cggccagcgt cgaaccactc cggcgcctcg ggaagcgcgc ggagtcgagc
     7261 gttcgcgctg gccggatgga cgtaggcgac ttggaagaga tccggccttt caccgagtgg
     7321 gccgtaggtg ccgcgcccct tgatactcgc gtcccacgcg ggcttcttca catggccgta
     7381 tgtggttatc cacttctgag cggagcatgt cccggtgagt atccggccgg agaacaggtc
     7441 ccgagcgtcg gaccccgtgt caccgaatag cttgagcgcc aattggtggg cgccgagcac
     7501 cacccggtgt tccaacgcgc gcccctgcat tagggtgacg taaagccaga aggggaccgg
     7561 gctttgcttc ttctgcttac cggggccggc ccaccactgc ttggcgcccc cggcgaacga
     7621 gccgaattcg tcgatgatga gcacgcgggc aggaatgtcc tccgtggaat acccctcctg
     7681 ctgcatcagc gcgacggcgg tagccgatgc gatgaattcc gccagcgcgc gaattgctcc
     7741 ctcaatcgtc gtgtgaatcc gcaccccgga cacggtccga gacggacggg tcgggtcacc
     7801 ggcgatcccg tccgggtgtt ccggcttccg ctccgtgaag ctcatacgct tgagatcgat
     7861 gatgtcgacc aacgcaccat gctcgcggtt gtgcaggaga ggaacggtca gggtggtggt
     7921 tttgccactc ccggaaccgg cgaccacggc caggtgaggc acgtccgtct cggttcgcgc
     7981 gtagacgggc gccttgccgg gaccggcgcc gatgtgcatc acgtacgggt cgtcggagga
     8041 ctcccactcg accgcgtccg ggggctgggg ggtgggcatg aactccatga accgcggcgc
     8101 actggtgagg tggacgcggg agacctccat cggcgccttg ctccaccggt cgatgagccg
     8161 gtgcacggca agctgctggt actcctcccc cacccagtgg tccgggaggt tcacgcgggt
     8221 gatggagccc tgcttggcag ggttccgcgg gacgatgagc cacttgtccg ccgacgcgcc
     8281 gatcgggagc tggagcggcc cggacaacga cttcatcagc ggctgaacga gccgtcgccg
     8341 gtgctggcgg tgcacccacc ggcggtgcgc ccggttaccc gcgtaggcca gcccaccggc
     8401 aacggcgacg gcggtcaggg aggtggtcag ggtcgggtcc gcgatctgcc cgtacgcctc
     8461 gcccaggacc gcgagggtgc cgccggtgcg gtgcgcggcg cgctgccacc ggggccggtg
     8521 ctgccaccgg tacgcgcggc cggaggggtg gatgacggtg gttgccgggt gaagccaggt
     8581 ggcgtcggtg cgggggactc cgtcgagcgg cgagccggag acccagcgcc acccgacgat
     8641 ccgggcgggg gttcgtgtgg aaaggtcatg tcgggccatg gggtgtgctc ccgtggtcaa
     8701 ggggtcgggc gcgtgggttc tttgctgaga gtcacgcgcc cgatcccgcc gagcaaaggg
     8761 gtttcgtccg gtctgtggat aaccgggatg atcaggactg atgcgccgga accgcggtcc
     8821 cgcggcccgg ggtggtggtg ctcgtgccat ccgccgcgcc ggagcgctcc gggcgcatcg
     8881 aacgagccgc gtggaacttc accgacacgg caacttcgtc cacctggacg tcccacccgt
     8941 agcgggtctc gccgctctgc ttgtcctccc aggacgactg acgcagcgtg cccaccacga
     9001 gcaccctgtc gcccttgcgc agcgactccg ccgcgttctc cgcggtctgc cgccacgcgg
     9061 tgcaccggtg ataggacgtc gccccgtccg accactcgcc ggtgttctgg tcacggcgac
     9121 gctcggtgca ggcgacgacg aacgacgcga cggccgcgcc gttcggggtg aaccgaagct
     9181 cagggtcgtt ggcgaggttc ccggcgatgg tgatcactgc ttcctgcatg gtgctgcctc
     9241 tctctgcgaa acggggatcg ggccgtgggt gggtgaggtc aggcgacgcg gggcacgcgg
     9301 gtacgggcga ggtggacgag tccggcgaca ccagcgagag cggccaggat cgccaccagg
     9361 gcgcccgtga ccacctccgg gtgtgcgagc accggccggc cgtagatgag gaccagggcg
     9421 acgctcaggg ccacgacggg ccggcggagc gtggcgagaa cggcgcgggc gaggtgcgcg
     9481 gcgcgctccg cgaggtggat accggccaag agcagaacga gaacggcgag aatggtcacg
     9541 ggacagttcc cttccgggtg caacggcggg gggtgttccg ggagcgggtg accgatgggg
     9601 tcggtcaccc gctccactgc tgccaagggg cagctcgggg ctacaggtac gggttcggct
     9661 cgtttgccgc ggcccgggac tcccggatga tccggcggac gtgcgagggg tcgaccgtca
     9721 cgccggagtc cgcgagacgc tcgcggatcg tggggacgtc ggtcactccg ctcgcgacaa
     9781 gctcacggac acgtcgggcc ttggacccgc ggggtgtccc gttgtccttc gggacgtccg
     9841 gggtgtcctc caccatgggg gcggacaagg ccgtgacctg ccggacttcg gtcaggacgt
     9901 cgacctgacg tcccacggga ggtgtggggg agtccgccac cgtggccacc tcccccacgg
     9961 gcaggccgac gacgtccgtc ccgatggggg gacgtcgcga tgcctccatc gcgagcatct
    10021 gcgccgtcgc ctggtcttcc acccgggcca cctgcctgcg aaccgcggcg acggccatct
    10081 gagcggacgc acgggagatc tgctgagcca cccactgtgc gtcgtccggc gacaggtccg
    10141 gggccgtcgc gcggttcagg gtccaccaca ggaccttcgc cgcgatgctg acggacgcgc
    10201 cgatgcacgc catggcaaca ccgccggtct ggagtccctc aagggcgatg gcggtcacgg
    10261 tgaccgcgag caacgcccat ccgatcttgt ccaccgtcgc gcgccggtcg ggccggaacc
    10321 ggacgatgaa cgacatgccg agcgtggcga gccacgcgag gtcgaacacc gcaccggcga
    10381 ggtaggccgt ccacgtgccg ttcaacaggt gcgcgatgga caccgtcgac cacgtgatgg
    10441 acaccaacgt gagcgcaagc accaggccga cgacaccgcg cacggcgata acgtcgatgt
    10501 cccgggggag caccggcacg gccacgcgac gggtacgggt gaccggcgcg gactcgccgt
    10561 tgacggtgtg ggtgtcggtg tacttctgcg tctcgacacg gaacttcatc gggtcacctc
    10621 gccgcgagct tcggtgacga gcgcggccag gcggacgaag tcccgcgcgt cgagcgcgcg
    10681 gcccatgtct cgaaccatcc cgcgcagcca gtccagatcg gccgagtcgg gggagacggg
    10741 catgtggtgg aagcggccgt ccggcgtgct ggcggggatg cagtagtcgc atccggatgg
    10801 ggcgggggtg aggtcgagct ctccgccgca cccctcgcag tggtcggggg gtcgtaggtt
    10861 tgtcatgggt cgggtccctt ctgtggaggg ggtcggtcaa gggccgtgtc cggggggcaa
    10921 tcccgggcgc ggcccgtctg gttacaggcc cttcgacttc ctgatctcgc ggaccaactt
    10981 cgcgcgctgg tcgagcccga gtgtcagcag tccgtcgacg acgtcggcct tggtcacgcg
    11041 cttgcccagt tccagggaca gttcgagggt gagccgctcg acagcatcga tgatcggctt
    11101 gctcaggtag taggacaccc gctcccgcgg gatctccgtc tgtacggtca ctcgtcgcct
    11161 cctcagcaag tccggcaggg tcggtgcttc tggagtcgac gatacctcag aagtcggaga
    11221 gtcggaggtt cggtgttcga ctttcggtgt tggatgtccg gattttggtt gcgagcgaac
    11281 gggggtcctt cgggtgcggg tgggtgccgg ccgcgcggga gtcggctccg gcgcggcctg
    11341 tccagtaggc ggtacggact ccggcgcggc gggcggctcg acagtcggtg tcgcgtccga
    11401 ttccggctcc gactccgggg cgagcatgtc ccggaacttc cggcgtccgg gcggtgtcat
    11461 ctgatgtctc ccgttacgta gtcgcggatg acctccgccg tcacccggcg gtagtcgaga
    11521 tcggcctcac tggcgtaccg gccgcgatag gccccgatgg gtactccggc cagtgcggca
    11581 tcttcgtgcg ccgcgtagct ccggatgatc gtgctgaaca cgccgtaccc ctgttccacc
    11641 aaatcggtcc gggcctgttc cgcccggggg atcgaccggg ggtccactcg ggtgaggaga
    11701 acccggaacg gcactccaga cggccggacc acatctcgga ccgttctggc gagtcctgcg
    11761 aggtccagtg ccgcgggtgg actcgggagg atgaccgcgt ccgcgtgacg caccacggca
    11821 agcagtgcgg cggactcccg ggcgcccggc gtgtccacaa cgatcacgtc gtactcggct
    11881 acctcgcgga cgtgccccaa gagtcgaggg tcggtttcgg cggtgtagtc gaagcggagc
    11941 tggacgccgg ccgcgcgcgc acggtccgcc cacctggcgg agtccccgtt ggggtcggtg
    12001 tccagtagca gcacgcgcgc ggcgagcgtc tcggcaagca agtgggtgat gttgagggcc
    12061 gacgtcgtct tgccgacgcc gcccttctga ctggcgaaga tcagtatccg catggcacgg
    12121 gccaccccct gtgttgtgaa catccacagt ctgatgtcgc acatcggagg tggacagggg
    12181 gatgcgcccg cgaggtgcga cacgcagtgt gggatgtccg tcgcgcccgg aacgtcagtg
    12241 ctactctgag cgttggaact aatcgcatgc gacttgtaga ggaggtgggg tagatgcaga
    12301 cgcgaacgcg atcagcgagg gtcccagacg atctttacct actcgcgatg caaca
//
