LOCUS NC_015632 14930 bp DNA circular BCT 15-JUN-2011 DEFINITION Methanothermococcus okinawensis IH1 plasmid pMETOK01, complete sequence. ACCESSION NC_015632 VERSION NC_015632.1 GI:336120990 DBLINK Project: 51535 KEYWORDS . SOURCE Methanothermococcus okinawensis IH1 ORGANISM Methanothermococcus okinawensis IH1 Archaea; Euryarchaeota; Methanococci; Methanococcales; Methanococcaceae; Methanothermococcus. REFERENCE 1 (bases 1 to 14930) AUTHORS Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Mikhailova,N., Held,B., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sieprawska-Lupa,M., Takai,K., Miyazaki,J., Whitman,W. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Complete sequence of plasmid of Methanothermococcus okinawensis IH1 JOURNAL Unpublished REFERENCE 2 (bases 1 to 14930) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (13-JUN-2011) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 14930) AUTHORS Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Mikhailova,N., Held,B., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sieprawska-Lupa,M., Takai,K., Miyazaki,J., Whitman,W. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Direct Submission JOURNAL Submitted (18-MAY-2011) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002793. URL -- http://www.jgi.doe.gov JGI Project ID: 4086330 Source DNA and organism available from William Whitman (whitman@uga.edu) Contacts: William Whitman (whitman@uga.edu) Tanja Woyke (microbe@cuba.jgi-psf.org) Annotation done by JGI-ORNL and JGI-PGF Finishing done by JGI-LANL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Methanothermococcus okinawensis IH1 collection_date :: Missing lat_lon :: Missing depth :: Missing alt_elev :: Missing country :: Japan environment :: Hydrothermal vent, Deep sea, Fresh water num_replicons :: 2 ref_biomaterial :: JCM 11175, DSM 14208 biotic_relationship :: Free living trophic_level :: Autotroph, Lithotroph rel_to_oxygen :: Anaerobe isol_growth_condt :: Missing sequencing_meth :: WGS assembly :: Newbler v. 2.3 finishing_strategy :: Finished GOLD Stamp ID :: Gi02044 Type Strain :: Yes Greengenes ID :: 48249 Funding Program :: DOE-CSP 2008 Gene Calling Method :: Prodigal 1.4, GenePRIMP Isolation Site :: Deep-sea hydrothermal vent chimney at the Iheya Ridge in the Okinawa Trough Japan Cell Shape :: Coccus-shaped Motility :: Motile Temperature Range :: Thermophile Temperature Optimum :: 40 - 75C pH :: 4.5 - 8.5 Gram Staining :: Gram- Diseases :: None ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Current Finishing Status :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: 454/Illumina ##Genome-Assembly-Data-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..14930 /organism="Methanothermococcus okinawensis IH1" /mol_type="genomic DNA" /strain="IH1" /isolation_source="deep-sea hydrothermal vent chimney at the Iheya Ridge in the Okinawa Trough" /db_xref="taxon:647113" /plasmid="pMETOK01" /country="Japan" gene complement(41..1315) /locus_tag="Metok_1622" /db_xref="GeneID:10749399" CDS complement(41..1315) /locus_tag="Metok_1622" /inference="similar to AA sequence:KEGG:MFS40622_1839" /note="KEGG: mfs:MFS40622_1839 integrase family protein" /codon_start=1 /transl_table=11 /product="integrase family protein" /protein_id="YP_004564626.1" /db_xref="GI:336120991" /db_xref="GeneID:10749399" /translation="MARHRAVKHQFLFAVTKSYKWKAPKKELKNQWRELDNQLKTGNI DYDTWVTKKANIWFISSSGDFFQGYKCKESYKESLQKFAKLFGDWIQKTYGIKYIKDI TPEMAQKFLETKAKEGVHYSTLRKYTAMIKALALASQKSFNKDLNNPSYIFNFAKGIH VPIDAVRCEDRTMRIDPAHYEKIINSDFFRNSKSTAKIGIPLARHFGLRVSELSRLCY NDIAMSFDEIPQKLQKFVIDKSPYDAYLIIRKGKGGVYRVIPALSKEEYILLSDIKKE CISKDINKNNWIIPVKRGAINKFFRECLKRNDLSYYIDHKISIHGLRKLRASELFLQK LGDYYLVEEIGNEKEKIYYTLKKAGKYVDRYLGHETSTKAKANQDTMRMKLVRRYQID VISPEDIASRLSECGADYNKALSIVNEILSEL" misc_feature complement(326..1099) /locus_tag="Metok_1622" /note="Site-specific recombinase XerD [DNA replication, recombination, and repair]; Region: XerD; COG4974" /db_xref="CDD:34580" misc_feature complement(326..712) /locus_tag="Metok_1622" /note="C-terminal catalytic domain. The tyrosine recombinase/integrase family share the same catalytic domain containing six conserved active site residues. The best-studied members of this diverse family include the bacteriophage lambda integrase, the...; Region: INT_REC_C; cd01182" /db_xref="CDD:29503" misc_feature complement(order(347..349,356..358,560..562,689..691)) /locus_tag="Metok_1622" /note="Int/Topo IB signature motif; other site" /db_xref="CDD:29503" misc_feature complement(order(347..349,356..358,689..691)) /locus_tag="Metok_1622" /note="active site" /db_xref="CDD:29503" misc_feature complement(order(347..349,689..691)) /locus_tag="Metok_1622" /note="catalytic residues [active]" /db_xref="CDD:29503" misc_feature complement(order(356..361,560..562,686..691)) /locus_tag="Metok_1622" /note="DNA binding site [nucleotide binding]" /db_xref="CDD:29503" gene 2640..5045 /locus_tag="Metok_1623" /db_xref="GeneID:10749390" CDS 2640..5045 /locus_tag="Metok_1623" /inference="similar to AA sequence:KEGG:MmarC5_1841" /note="KEGG: mmq:MmarC5_1841 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004564627.1" /db_xref="GI:336120992" /db_xref="GeneID:10749390" /translation="MATSINTFPKELFNQNTNIKESTNILYKKEISTNEMDYFIGSYS DINKPIFKRLRKIYPFLDEELQKIATSSNKHNIKIIIGLQLVLAYDHQEYKLYLQNNI KLIDNETTGACTYNHTILDENSDKTLVFAFQNAEEIIDLFLKNKYYYFKNVFTYKYVP KVEDYKNNDIYAIQKYISIDATKIIKKMDGIRYEILKMLYGFLKDRIIYKSSALILAE YNNKIIFPEVEKAPEMHDYNAIPTPEELYHELSNINIDDNKKRMLLTELTKLNKDHNI LTMAYYMGTLLIPIINYETTPYLILYGDSNCGKTKTANLFNFANIQSDRTTTTQIMRT CHGYGCGYFVLDEPKTISNDVLQMLKDISTKKRYTKSYGKTGQKYIFKMGGIISANSI YEIRTKSPEDMQAFIRRNIVLKLNEDDKLTNINNVVRKLEKENLSLKKIFIDFLMSKN PQELIIQYESIDTDEEQYKFIIFGLRLLKELFDSFGINFLTDERINEIINRLKETERE FQQDILKDDNIVEMIEQIIYNNTINTISLDNKNPCDLTDTKVLNEYTDILYRRYGYTI YYMDDKKRIAINKVGQLKLIKTLNKQYGTNYPEQTTQEWLAEKLKEKGIYVEISRKRI SNRPDAIIPRCLFIEIPYNYDLQDYSNLIDIIIPMCEIGQGWINTQELKEKATVKSIE LYKVSECMNLLKHYNLIEEEKEENCKINIKKLKNMVEIGDESGGKSRDMSLPGKKEQP CGDKVFYVMTTLTSIFGSDPIDYDTIKEQSGMDEEELDKALTKLKWDGYIDEPRPGKY RMI" misc_feature 3834..>4166 /locus_tag="Metok_1623" /note="Ferredoxin reductase (FNR), an FAD and NAD(P) binding protein, was intially identified as a chloroplast reductase activity, catalyzing the electron transfer from reduced iron-sulfur protein ferredoxin to NADP+ as the final step in the electron...; Region: FNR_like; cl06868" /db_xref="CDD:195401" misc_feature order(4158..4160,4164..4166) /locus_tag="Metok_1623" /note="conserved FAD binding motif [chemical binding]; other site" /db_xref="CDD:99778" gene 5124..8597 /locus_tag="Metok_1624" /db_xref="GeneID:10749398" CDS 5124..8597 /locus_tag="Metok_1624" /inference="similar to AA sequence:KEGG:Mbur_2163" /note="KEGG: mbu:Mbur_2163 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004564628.1" /db_xref="GI:336120993" /db_xref="GeneID:10749398" /translation="MVNYDYKFLKDKGFFKNQREYDENGTNIIVDMDNYIVYVEVNND DKLNELKKAILEKYPKIDVIYFHYPADNKIKVFRKWGEVKWFYYSDNLRNDRKKSKKD KLKKFAPDNVSILFDIKDVMDRFYKDLWVHRLEMAKSITEPLTDSDKLFIAQHFIDRL VFFYFLAQLGIININIKYKGELHNIKLNKKGTREFFNYLLNIVGDEGLHYILNKIFFN GLGNGEDVDKNGFVVLSFSIKDTDIDIKVPYLNGGLYRVKKFNDIDETEIKFNGLKNL IETLNKYNWVIGDYTEEDDDSIGSISPEIMGHVYEKFVVGLENIGEIELDKIEIGKEV KIGRKKVGAFYTPEEITKYISKNTITPYLFDKLEVSKHYKDFDDFVDNASKEELKKAL SVLNDIKILDPACGSGHFLVCAGELLFNMKNTICNKLYEKDGSFIRKHNAYDEVKNII VNNLCGVDICNSAVEIAKLRLWLWLVSQLKDDSSKLEPLPNLEYNIKCGNSLIGWVDE PLVLPNYENDSLINRKKIEEKLNELNSLIIEEKLNKLNSLKVCEDEKEKNELNSLIKV CEDEKEKIEKVRKLLNKKVGTKKREKFDDIYVEAFHLLYLIYRTSHGGKAGILKTILE EIRKEIYNVINNCYLNYINSKLKAKDKISEKDFLNLNPFHWRIDFGWIVKNGGFDIVI GNPPYGNLLSNVEKLIVEKIYMSDTSEIAGVFVERGINVLENNGYLSYIITFAITFNK KLSKTREILRKNFEKIFISSFDRDKCRFFEGMSQSVSILRAKIKKINGGGTFYTSKMF REMPKNLNNIKYSPANEYLLLNNEDIGNRFDIQHRLPKIGGLLNLKILDTLLKNTNKA KNVIGTGCSTIWIRTSGNYWYNAWDKKPYESTEIKSMSVKEVWSNFIMLFMNSSLFYW WFRIYGDGRHMNMDILKAIPLPNEDTIKKDAKLLECVRNITMDKLFSVFDSGRNRFNT SDVKGYLDLIDLIIGKKYYNLQPYEIIHILNTDYEVRGGIKLYSPFYELIHYLAFLTL KKQKEDTDTINFMDIIINNLIYELYFKEKFYEDKIYNEPKEYLLEAVSKHLEPINYDK WAELYWKKQLENNLTSEEEQELKQLEENNIKTVKEVYNSIKEDKEINELINKIKSHKW VKIIECVNNSI" misc_feature 7155..>7379 /locus_tag="Metok_1624" /note="S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy)...; Region: AdoMet_MTases; cl12011" /db_xref="CDD:196296" gene 8845..9282 /locus_tag="Metok_1625" /db_xref="GeneID:10749391" CDS 8845..9282 /locus_tag="Metok_1625" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004564629.1" /db_xref="GI:336120994" /db_xref="GeneID:10749391" /translation="MNKKDSSYLESNLEKEKKISLEIDPEVDRLLRDYQYKPIKEFER WVNETIKKIESSDVGLSSEGEAKIMVGYLRQCISVKASTVWQLPRFMIDNDEMLRFEQ LKSKLEITIALARDRYKVNKRKDIVKVVKSIATSVTNLLHKLP" gene 9590..12637 /locus_tag="Metok_1626" /db_xref="GeneID:10749392" CDS 9590..12637 /locus_tag="Metok_1626" /inference="protein motif:PFAM:PF00271" /note="KEGG: mbu:Mbur_2162 helicase-like protein; PFAM: DNA/RNA helicase, C-terminal; SNF2-related; SMART: DNA/RNA helicase, C-terminal; DEAD-like helicase, N-terminal" /codon_start=1 /transl_table=11 /product="helicase domain-containing protein" /protein_id="YP_004564630.1" /db_xref="GI:336120995" /db_xref="GO:0003676" /db_xref="GO:0003677" /db_xref="GO:0004386" /db_xref="GO:0005524" /db_xref="InterPro:IPR000330" /db_xref="InterPro:IPR001650" /db_xref="InterPro:IPR014001" /db_xref="GeneID:10749392" /translation="MQSLQTIPIYNNKPGIVSNEDTVLNLFKSKIRDAIKHKKEISIY CAVGFFFFNGLSELIEDLRKLYDKNLVKEFKLLMGRETTRDTKEMLKLITNDATNLTN EDYLFLKELHNKNIFQFKIYTDKRFHSKMYIFKANNIIEDVYAGSANLTRAGLTNNIE LVVPTGASNIERENYEIFFNELWKKGTDELEELKTIDIIKQGATTKAFYLPPKEFFCN LLKIMDKEYLLSNPSLEIEYLAEFQKMSYYYCLEKLHNYGGCILANSVGLGKTDVACA ISKYYNEMNKKVLIIHPPNIKHQWIGILKKVGLNPYKDVDLLSMGVLQSKDFKPYNYN KNYDLIIIDEAHNFRNRSSNRRKNLDEVIKINQNIHTLLLSATPINTSLDNYISLIEL FTLKPSYNSKFENMGILTKINTAKKYIKNKDTINAVTTVRDLIKEFTVRIEWIDVINH FKDDLIKIAGIDNFEMPEVQPVEYSYNKDIVKAVFDRVVNYLEHLNFEYAKLWDEEGY KEDKYLLFWYKWRLYKRLESSIYAFKKSLKKYIERNEYLLNAFECINHNKLVTTPDIE KLFPKDRLETIYSTYSSLDDDIKKTIMNNIKNDIEITKIMLERVNSIENLLKKDDKVR VLINILKKENKPTIIFSESKDTVLYLKSKLESNGFSNIGVAYGGDKGGKINKKKIQES FNNGKFDILITTDTLSEGVNLPRADVVINFDLPYNPVRLIQRAGRAIRLNNPKHIKIY NFKPDESIDKELELCERLKERVENIAVTVGLEFLIWAVEQKKIDKFSDENRELVYNCI KEYKNKLASSGLEELERSIGNAVSKEDYVLREYINQYNISKESILIYYKKYSKPIYTS LKRDYKEDDYFVVLKYRGNNYYCGELSYHPIEYKSPLTNKDFKIITEMINSKVNEIDN MFIGTKVRVDKLTREIKKLCNEDNQLRRWIISKEGQINTLPRADKEKVIAKLKEYNEA PEIFKSNDKSLKIITDLNSIVNKRNVQITMDKPELVAVIKYKV" misc_feature 9662..10138 /locus_tag="Metok_1626" /note="Catalytic domain of phospholipase D superfamily proteins; Region: PLDc_SF; cl15239" /db_xref="CDD:197447" misc_feature order(9971..9973,9977..9979,10028..10030,10034..10036, 10067..10069) /locus_tag="Metok_1626" /note="putative active site [active]" /db_xref="CDD:197200" misc_feature 9971..9973 /locus_tag="Metok_1626" /note="catalytic site [active]" /db_xref="CDD:197200" misc_feature 10310..11779 /locus_tag="Metok_1626" /note="ERCC4-like helicases [DNA replication, recombination, and repair]; Region: MPH1; COG1111" /db_xref="CDD:31308" misc_feature 10364..10723 /locus_tag="Metok_1626" /note="DEAD-like helicases superfamily. A diverse family of proteins involved in ATP-dependent RNA or DNA unwinding. This domain contains the ATP-binding region; Region: DEXDc; cd00046" /db_xref="CDD:28927" misc_feature 10388..10402 /locus_tag="Metok_1626" /note="ATP binding site [chemical binding]; other site" /db_xref="CDD:28927" misc_feature 10616..10627 /locus_tag="Metok_1626" /note="putative Mg++ binding site [ion binding]; other site" /db_xref="CDD:28927" misc_feature 11426..11809 /locus_tag="Metok_1626" /note="Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may...; Region: HELICc; cd00079" /db_xref="CDD:28960" misc_feature order(11513..11524,11591..11596,11669..11677) /locus_tag="Metok_1626" /note="nucleotide binding region [chemical binding]; other site" /db_xref="CDD:28960" misc_feature order(11693..11695,11756..11758,11768..11770,11777..11779) /locus_tag="Metok_1626" /note="ATP-binding site [chemical binding]; other site" /db_xref="CDD:28960" gene complement(12679..12969) /locus_tag="Metok_1627" /db_xref="GeneID:10749393" CDS complement(12679..12969) /locus_tag="Metok_1627" /inference="protein motif:PFAM:PF05016" /note="KEGG: mja:MJ_1173 hypothetical protein; manually curated; PFAM: Plasmid stabilisation system" /codon_start=1 /transl_table=11 /product="plasmid stabilization system" /protein_id="YP_004564631.1" /db_xref="GI:336120996" /db_xref="InterPro:IPR007712" /db_xref="GeneID:10749393" /translation="MDADLLNLLKKMAKKDRKRVEIILKKIDEIVENPHHYKNLRKPM EDFKRVHIDKSFVLIFTVDDNEKKVVFVDFAHHDEIYTNKRLSNIMEKLHRE" misc_feature complement(12727..12945) /locus_tag="Metok_1627" /note="Plasmid stabilisation system protein; Region: Plasmid_stabil; cl11422" /db_xref="CDD:196226" gene complement(12977..13219) /locus_tag="Metok_1628" /db_xref="GeneID:10749394" CDS complement(12977..13219) /locus_tag="Metok_1628" /inference="similar to AA sequence:KEGG:MJ_1172" /note="KEGG: mja:MJ_1172 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004564632.1" /db_xref="GI:336120997" /db_xref="GeneID:10749394" /translation="MVKAIVNISDENNQIINIIKAKYNLKDKSEAINKIIEEYTELLL EPELKPDYVEKIKKIMERNNMVEFDSVDELRRELGL" misc_feature complement(12980..13219) /locus_tag="Metok_1628" /note="Protein of unknown function (DUF2683); Region: DUF2683; pfam10884" /db_xref="CDD:151333" gene complement(13491..13850) /locus_tag="Metok_1629" /db_xref="GeneID:10749395" CDS complement(13491..13850) /locus_tag="Metok_1629" /inference="similar to AA sequence:KEGG:Arcpr_0565" /note="KEGG: apo:Arcpr_0565 transcriptional modulator of MazE/toxin, MazF" /codon_start=1 /transl_table=11 /product="transcriptional modulator of MazE/toxin, MazF" /protein_id="YP_004564633.1" /db_xref="GI:336120998" /db_xref="GeneID:10749395" /translation="MDFKQGDIVLLPFPFDDLTTIKKRPALVVSSNKFNRISKTVIVV EITSNVNSGFQELNVLIDNKDVELYPGAKPIIPSVVKPYVIFSINTELIIKRIGMLKE KKLEEVFSRLKQVVSYP" misc_feature complement(13503..13841) /locus_tag="Metok_1629" /note="PemK-like protein; Region: PemK; cl00995" /db_xref="CDD:194001" gene complement(13862..14005) /locus_tag="Metok_1630" /db_xref="GeneID:10749396" CDS complement(13862..14005) /locus_tag="Metok_1630" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004564634.1" /db_xref="GI:336120999" /db_xref="GeneID:10749396" /translation="MTTTIEMPTKNVKKEKLSKEDKWVLKMVQPILAKDWNSKEDEYW DDY" gene complement(14169..14618) /locus_tag="Metok_1631" /db_xref="GeneID:10749397" CDS complement(14169..14618) /locus_tag="Metok_1631" /inference="similar to AA sequence:KEGG:Mefer_1614" /note="KEGG: mfe:Mefer_1614 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004564635.1" /db_xref="GI:336121000" /db_xref="GeneID:10749397" /translation="MRGKIKKWLGIDKLEHKVDELNNEKLVILKTIGEVRNNINLLKN ELNELRLCKDNIDDVCNRIVEIENKLNELESFHNSLNVISANDNKNNIEDTILNIISN GKDVCISDLLTYTNLTTRELYNILSKLESLGKIKRKRKGKKVIVEGI" ORIGIN 1 caatttaatg aatgattttt atggttttat aatatggatt ttagagctcc gataaaattt 61 cattaacaat actcaaagcc ttattataat ctgcaccgca ttcagacaat cgggaagcta 121 tatcctctgg tgaaataaca tcaatttggt atcttcttac caatttcatt ctcatagtat 181 cttgattagc tttggctttt gtggaagtct catgtcctaa gtatcgatcc acatattttc 241 cagctttttt tagtgtataa tatatttttt ctttttcatt tcctatttct tcaaccaggt 301 aataatcacc taatttttgc aggaatagtt ccgatgctcg aagttttcta agtccatgga 361 tgctaatttt atgatctata taataactta aatcattcct ttttaaacat tctcgaaaaa 421 acttattaat tgctcctctt ttaacaggga taatccaatt atttttattt atatctttgg 481 aaatacattc ttttttaata tcacttaata aaatatattc ttcttttgac agtgcaggga 541 taaccctata aactccacct ttgcccttcc taattataag gtatgcatca taaggggatt 601 tgtcaattac aaacttttgt aatttttgag gtatttcgtc aaaactcatg gcaatatcat 661 tataacacaa tcgtgacaac tcagatacac gaagaccaaa gtgacgagct aagggaatac 721 ctattttagc agttgatttg gaatttctaa aaaaatcact gtttattatt ttttcatagt 781 gagcgggatc tattctcata gtcctatctt cacatctaac agcgtcaata ggaacatgaa 841 tcccttttgc aaaattaaaa atgtagcttg gattattcaa atctttatta aaggattttt 901 gtgaagccag agcaagggct tttatcattg cagtgtattt cctcaatgtg ctataatgca 961 ccccttcttt tgcttttgtt tcaaggaatt tttgagccat ctcaggtgtt atatccttaa 1021 tgtattttat gccataagtt ttttgaatcc agtcaccaaa cagttttgca aatttttgaa 1081 gtgattcttt ataactttct ttacatttgt atccttggaa aaaatcacca ctcgacgaaa 1141 taaaccaaat attagctttt ttagttaccc acgtatcata gtctatgttg ccagtcttta 1201 attgattgtc aagctccctc cattgatttt ttaactcttt ttttggagct ttccatttat 1261 aggatttagt aaccgcgaat aaaaactggt gttttactgc tctatgtctt gccatgttat 1321 cacttaattt caatataata atatatcaaa ataataaaaa tagtcctttt ttcgctcgtc 1381 agcgagtaag tggggcttac tcgctaccga taccccctta aattaatgtc cccagtatag 1441 tagtaacaat ataaaaaaca ctcttgaacc cacaaggctc aatatcagag cctacaagca 1501 ccatggaaca ggcggaatat tccagtcccc attccagctc ctccctcatc ttatcatatt 1561 aatatatgat aaggtcattg atcatcggag ctactttaaa atttaaattt aaaaatagat 1621 ttttttcaaa agattcttta gttttgtatt taatatttat attatttttt tcttttcaat 1681 ttttcgccaa caaatttttt gatttgatat ttatacaatt ataaagtaca ctatttaagt 1741 atataaatct aacggaaaaa gccttatagc atgtttttat cgtatataaa tgtttctatt 1801 ttaaaaaaaa cgctaaaaca ttggaaaaat tacaacatac aagtaataat aaatatgaaa 1861 aatttaacaa aatataatat taaaatagaa tgttccacaa atgttccaat acattatgga 1921 acataaaaca agaataatcc atcgataaaa ctactaaaat aagataaata tatcgttaaa 1981 aaaaatgttc catatttttg tgttttctat gtatatattt ttagaaaaaa atttaataat 2041 aatactaaaa aaaatattaa aaaattaatt aaaaattttt ataacaaaat attttggaac 2101 atttggaaca tttggaacat aaaaataaat aagaaaaaat aacggaataa acaataaata 2161 aaagccaaat taataatatt ttatgttcct tatatatgtt ccatacaatg aacatttgga 2221 acattgtata ttatattaat tttaaacttt gaatttctaa aaaaaacttc aaaaatagca 2281 atcgtttaaa attcatatag catgttttta ttgtatataa atgtttgcat aattcttatt 2341 ttaaaaaaat aaatatgcta tggagcctat gcttgaaaaa aagcaaaagg tttaaatatt 2401 aaaaatgtaa aaatagtata tattaaataa ttaaattgta gatgttggct gcatctacat 2461 gaaaaacacc atatattggt ttattttatt aattatcaat aaattaatag ttcaatctaa 2521 tttaacacac ggtgatatat ttacaactaa acacactgtg tatgtaatta attactgtat 2581 atgtaaataa ctgattaatt aaacaactaa acacagtgat taaacacggt gatatatcta 2641 tggctacttc tataaatact tttccaaagg aactatttaa tcaaaataca aacataaagg 2701 aatcaacaaa tatattatat aaaaaagaaa tctctacaaa tgagatggac tattttatag 2761 gctcatatag tgatattaat aaacctattt ttaagagact tagaaaaatc tatccttttt 2821 tagatgagga attgcaaaaa atagcaacat ccagcaataa acacaatata aaaataatta 2881 taggattgca attagtattg gcttatgatc atcaagagta taaactatac ctgcaaaaca 2941 atatcaaatt aattgataat gagacaactg gggcttgcac ctataatcat actattttag 3001 atgaaaattc agataaaaca ttagtctttg catttcaaaa tgcagaggag ataatagact 3061 tatttttgaa aaataaatat tattacttta agaatgtttt tacatacaaa tatgttccaa 3121 aagtagaaga ttataaaaat aacgatatat atgccattca aaaatatatc agcatagatg 3181 ctactaaaat aattaaaaaa atggatggta taaggtatga aatattaaaa atgctgtatg 3241 ggtttttaaa ggatagaata atttataagt caagtgcttt aattttagca gagtataata 3301 ataaaataat attcccggaa gtagaaaaag caccagagat gcatgattac aatgcaatac 3361 ccacaccaga ggaactatat cacgaattat ccaatataaa cattgatgat aataagaaaa 3421 gaatgttatt aacggagcta actaaactaa ataaagacca taatatttta actatggcat 3481 attatatggg aacgctcctt attcctatca ttaattatga aactacaccc taccttatat 3541 tatatggtga ttcaaactgt ggaaagacta aaacagcaaa cttatttaat tttgccaata 3601 tacagtcaga tagaaccaca acaacacaga ttatgagaac atgccacggc tatgggtgtg 3661 gttattttgt attggatgaa cctaaaacta tatccaatga tgtattgcag atgttaaaag 3721 atatatcaac taaaaaaaga tatacaaaat catatggaaa aacaggacag aaatatatat 3781 ttaaaatggg cggtataatc tcagcaaatt caatatatga aattagaacc aaaagccccg 3841 aggacatgca ggcatttata aggcgtaata ttgtattaaa actaaatgaa gatgataaac 3901 taactaatat taataatgta gttaggaaat tagagaagga gaatttatca cttaaaaaga 3961 tattcataga ctttttaatg agtaaaaatc cacaggaatt aataatccaa tatgaaagca 4021 tagataccga tgaagaacaa tataaattta taatttttgg tttaagacta ttgaaagaac 4081 tatttgatag ttttggaatt aactttttaa ctgatgaaag aataaacgaa ataataaaca 4141 gattaaaaga aacagaaaga gaattccagc aagacatact aaaagatgac aatattgtag 4201 agatgattga gcaaataata tacaataaca caataaatac catctccttg gataataaaa 4261 acccctgtga tttaactgat acaaaggtat tgaatgaata tactgatata ttatataggc 4321 ggtatggtta cacaatatat tatatggatg ataaaaaaag aatagcaata aataaggtag 4381 gacagctaaa actaatcaaa acattaaata aacaatatgg cactaactat ccagaacaaa 4441 ccacacaaga gtggcttgct gaaaaattga aggaaaaagg aatatacgtg gaaatatcaa 4501 ggaagagaat ctcaaacaga cctgatgcta taataccaag atgtttattt attgaaatac 4561 cttacaatta cgatttacag gattattcaa acttaattga tataatcatc cctatgtgtg 4621 agattggaca aggttggatt aacacccaag aattaaaaga aaaagcgaca gttaaaagta 4681 tagaattgta taaagtaagt gaatgtatga atttattgaa gcattataac ttaattgaag 4741 aggaaaaaga agaaaattgt aaaataaata ttaagaaatt aaaaaatatg gtggaaatag 4801 gagatgaatc agggggtaaa tcaagggata tgtctctacc aggtaaaaaa gaacaacctt 4861 gtggtgataa ggtattctat gtgatgacaa cacttacaag tatatttgga agtgatccaa 4921 tagattacga tactattaaa gagcaatctg gaatggatga agaagaattg gataaagcat 4981 taaccaagtt aaaatgggat ggttatattg acgagccacg ccccggaaaa tataggatga 5041 tttaaatgtt atttatttta tgttattgtg ttgggaacta acatgttgat atttatatat 5101 tactacttat tttaggtgag ctcatggtaa attatgatta caaattttta aaagataagg 5161 gattttttaa aaatcaaagg gaatatgatg aaaatggcac caatattata gttgacatgg 5221 ataattatat tgtctatgtt gaagtaaata atgatgataa gctcaatgaa ttgaaaaaag 5281 caattttaga aaaatatcca aaaatagatg tgatttattt tcactatcca gcagataata 5341 aaataaaggt atttagaaaa tggggagagg ttaagtggtt ttattattct gataatctaa 5401 gaaatgatag gaaaaaaagt aaaaaagata aattaaaaaa atttgcaccc gataatgtaa 5461 gtatattatt tgatattaag gatgtaatgg atagatttta taaagattta tgggtacata 5521 ggttagaaat ggctaaaagt ataactgaac cactcacaga tagtgataaa ttattcattg 5581 ctcagcattt tattgatagg ttggtatttt tctacttttt agcacagttg ggaattatta 5641 atattaatat aaaatataaa ggtgagctcc acaatattaa actaaacaag aagggaacaa 5701 gggaattctt taattatctg ttgaatatcg ttggagatga agggttacac tatatattaa 5761 ataagatatt ttttaatggg ttaggtaacg gagaagatgt ggataaaaat ggttttgttg 5821 ttcttagttt ctcaataaag gatacagata ttgatattaa agtcccatac ctaaatggtg 5881 gattataccg agtaaaaaaa tttaatgata tagatgaaac agaaataaaa ttcaatggac 5941 ttaaaaactt aattgaaacg ctaaataaat ataattgggt tataggcgat tatacagaag 6001 aggatgatga ctcaataggt agcatatcgc cagagataat gggacatgtt tatgaaaaat 6061 ttgttgtagg tttggagaat attggggaaa tagaattgga taaaatagaa attggtaagg 6121 aggttaaaat tggtagaaaa aaggtagggg cgttttacac tcctgaggaa ataactaaat 6181 atatttcaaa aaacactatt acaccatatt tatttgataa attagaagtt agcaaacatt 6241 ataaagattt tgatgatttt gtagataatg cttcaaaaga ggagttaaaa aaagcattaa 6301 gtgttttgaa tgatattaaa atattagatc cagcatgtgg tagtggtcac ttcttagtat 6361 gtgcagggga gctcctattc aatatgaaaa atactatttg taataaatta tatgaaaaag 6421 atggttcatt tattagaaag cataatgcct atgatgaagt taagaatatt atagtgaata 6481 atttatgtgg tgtggatatt tgtaatagtg ctgttgagat tgctaaatta agattgtggt 6541 tgtggttggt aagtcagtta aaggatgatt cttctaaatt agaaccttta cctaacttgg 6601 agtataatat taaatgtgga aactcattaa ttgggtgggt tgatgaacca ttagttttgc 6661 ctaactatga gaatgatagt ttaattaata ggaaaaaaat tgaagaaaag ttaaatgaat 6721 taaattcatt aataattgaa gaaaagttaa ataaattaaa ttcattaaaa gtttgtgagg 6781 atgaaaaaga gaaaaatgaa ttaaattcat taataaaagt ttgtgaggat gaaaaagaga 6841 aaattgaaaa agttaggaaa ttacttaata aaaaagtagg aacaaaaaag agagagaaat 6901 ttgatgatat ttatgttgaa gcatttcact tactatactt aatatataga actagtcatg 6961 ggggtaaggc aggaatctta aaaactattt tagaagaaat cagaaaagaa atttataatg 7021 taattaataa ttgttatttg aattatatta acagtaaatt aaaagcaaaa gataaaattt 7081 cagaaaaaga ttttttaaat ttaaatccat tccattggag gattgatttt ggttggattg 7141 ttaaaaatgg tggttttgat attgtaattg gtaatccacc ttatggaaat ttattatcaa 7201 atgttgaaaa attaatagtt gagaaaattt atatgtcaga tacttctgaa attgcgggag 7261 tatttgtaga gagagggatt aatgttttgg aaaataatgg gtatttgtca tatatcataa 7321 cctttgccat aacatttaat aaaaaactat ctaaaactcg tgaaatttta agaaaaaatt 7381 tcgaaaaaat atttatttca tcatttgata gggataaatg tagatttttt gaaggcatgt 7441 ctcaatcagt atcaatactt agagctaaaa ttaaaaaaat aaatggtggg gggacatttt 7501 atacaagcaa gatgtttaga gaaatgccaa aaaatttaaa caatataaaa tattctccag 7561 ctaatgaata tttattgtta aataatgaag atatagggaa cagatttgat atacaacata 7621 ggttgccaaa gattggagga ttattaaatc ttaaaattct tgatactttg ttaaaaaaca 7681 caaataaagc aaaaaatgta attggtactg gatgttcaac aatttggata aggacaagtg 7741 gtaattattg gtataatgct tgggataaaa aaccttatga aagcactgaa ataaaatcaa 7801 tgtctgtaaa agaagtatgg agtaatttta ttatgttgtt tatgaattct tcattattct 7861 attggtggtt caggatatat ggggatggga gacacatgaa tatggatatt ttaaaagcta 7921 tacccctacc taatgaagat actataaaga aagatgctaa attattggaa tgtgttagaa 7981 atattacaat ggataaattg tttagtgttt ttgatagtgg aagaaatagg tttaacacaa 8041 gtgatgtaaa aggctattta gatttgatag atttgattat tgggaagaag tattataatc 8101 tacaaccata tgagataata catatattaa atactgacta tgaagttagg ggcgggatta 8161 aactatattc tccattttat gaattgatac attatttggc atttcttact cttaaaaaac 8221 aaaaagagga tacggataca ataaacttta tggatataat aatcaacaat ttaatatacg 8281 agctctattt caaagaaaaa ttctatgagg ataaaatata caatgaacca aaagaatatt 8341 tattggaagc agtttcaaag cacttagaac caataaatta tgataaatgg gctgaactat 8401 attggaaaaa gcaattagaa aataacttaa catcagaaga agagcaagaa ttaaaacaat 8461 tagaagaaaa taatataaaa acagtaaaag aagtttataa ttctataaaa gaagataaag 8521 aaattaatga gttaattaac aaaataaaat ctcataaatg ggttaagatt attgaatgtg 8581 taaacaactc catttaaaat tacaatatat aataattata cataactttt tttagttttt 8641 tgaagcatgc tgatgttcgt atgtgttatt ccgaactcat ggcttagtta ataggaaata 8701 aacgaaaatc gagccataat cattatatat tatatatact aatatttaga tagattgaag 8761 ataaataata agatttttaa aatgccaaat ccttttgtaa agaatggaaa agttcaaatt 8821 gaaaaatgtt attaaggaga aaaaatgaac aaaaaagatt cttcttattt agaatccaat 8881 ttagaaaaag aaaaaaagat atccttagaa attgatccag aagttgatag gctgttgagg 8941 gattatcaat ataaacctat taaggagttt gaaagatggg ttaatgagac aattaaaaaa 9001 atcgaatcat cagatgtagg gttgagctca gaaggtgaag ctaaaataat ggtggggtat 9061 ttgcgtcaat gtatatcggt taaggcatcc acagtttggc agttgcctag atttatgatt 9121 gataatgacg agatgttgag atttgaacaa ttaaaatcta aattggaaat cacaatagca 9181 ctggcaagag ataggtataa agttaataaa agaaaagata ttgtaaaagt ggttaaatct 9241 attgctacat ctgttaccaa cttattgcat aaattaccat aaaatcttca tgtattaaaa 9301 ttgacaatac aagcatttat atttttagtt cttcttttga gacatatggc cacattttaa 9361 tatatctttg tatgtatcta aatattttca ataacttaat tttatagtat aatacattgg 9421 tataatatgg atggttcttt gaagataatt caaaagaatt tcgacattca tagtataatg 9481 ttagtctaat tctaattgct attttctgac atataactgc cctttaaaat aatatatatg 9541 tttttaattt ttgatatata cttataatta aaatcttaaa ggtgagctca tgcaatctct 9601 tcaaactatc cctatctata acaataaacc aggtatagtc tctaacgaag atacagtatt 9661 aaacttattc aaatcaaaaa ttagggatgc aataaaacat aaaaaggaaa tatccatata 9721 ctgtgcagtg gggttcttct tttttaatgg tttatcggaa ttaatcgaag atttaagaaa 9781 attatatgat aaaaatttag taaaagagtt taagctttta atgggaaggg aaacaacaag 9841 agacacaaaa gagatgttaa agttaattac aaatgatgct acaaacctaa ctaatgaaga 9901 ttacctattt ttaaaagaat tacacaataa aaatatattt caatttaaaa tttacacgga 9961 taaacgattt cacagtaaga tgtatatttt taaggcaaac aatatcatag aagatgttta 10021 tgcagggagt gcaaacctta caagagcagg actaaccaac aatatagagt tggtagttcc 10081 aacaggagca tcaaatattg aaagagaaaa ctatgaaata ttttttaacg aattatggaa 10141 aaaagggact gatgaattag aagagttaaa aacaatagac attattaaac agggagcaac 10201 taccaaagca ttttaccttc caccgaagga gtttttttgt aatctattaa aaataatgga 10261 taaagaatac ctgctatcaa acccatcatt agaaattgaa tatttagcag aatttcaaaa 10321 gatgagttat tattactgtt tagaaaaact ccacaattat ggaggttgta tcttagcaaa 10381 ttctgttggg ctggggaaaa cagatgtagc atgtgccata tcaaaatact acaatgaaat 10441 gaataaaaaa gtattgataa ttcatccacc aaatataaaa caccaatgga taggaatatt 10501 aaaaaaggta gggttaaatc catacaagga tgtagattta ttatcaatgg gtgtattaca 10561 gagtaaagac tttaaacctt acaattataa caagaattac gatttaataa tcatagatga 10621 ggctcacaat tttaggaata ggagctccaa cagaagaaaa aatttagatg aagttataaa 10681 aattaaccaa aatattcata cattactatt atctgcgaca cctattaaca cttcgttgga 10741 taattacatt agtttgattg aattatttac cttaaagccc tcatataata gtaaatttga 10801 aaacatgggg atattaacca aaataaacac cgctaaaaaa tatattaaaa ataaggatac 10861 aataaatgcc gtcaccacag ttagagattt gattaaggaa ttcactgtaa gaatagaatg 10921 gattgatgta attaatcact ttaaggacga tttaattaag attgcaggta ttgacaactt 10981 tgaaatgcca gaggttcagc cagtagaata tagttataat aaggacatcg ttaaggcagt 11041 ttttgatagg gttgtaaatt atttagaaca tttgaatttt gaatatgcta aattatggga 11101 tgaagaagga tataaggaag ataaatatct tctcttttgg tataaatgga ggttatataa 11161 acgattagaa agcagtattt atgcttttaa aaagagttta aagaaatata ttgaaagaaa 11221 tgagtattta ctaaatgctt ttgagtgtat aaatcataat aagttagtaa ccaccccaga 11281 tatagaaaaa ttattcccta aggataggtt agaaacaata tattctacat atagtagctt 11341 agatgacgac ataaaaaaga caataatgaa caatattaaa aatgatattg aaatcactaa 11401 aatcatgctg gaacgagtaa atagtataga aaatctatta aaaaaagatg acaaggttag 11461 agtattaatc aatattctta aaaaagagaa caaaccaact atcatctttt cagagtcaaa 11521 agatacagta ttatatctta aaagtaaatt ggaatctaac ggatttagca atataggggt 11581 agcttatgga ggagataaag ggggaaaaat aaataaaaag aaaatacagg agtcatttaa 11641 caacggtaaa tttgatattc ttataactac tgatacacta agtgaaggag ttaatcttcc 11701 aagggcagat gttgtaatta actttgattt accatataat cctgttagat taattcaaag 11761 ggcagggcgt gctattagat tgaacaaccc aaagcatatt aaaatataca atttcaaacc 11821 tgatgaaagt attgacaagg agttagagct ctgtgagagg ttaaaagaaa gggttgaaaa 11881 tattgcagtt acagttggat tggagttttt aatttgggca gttgaacaga aaaaaataga 11941 taagttttca gatgaaaata gagaacttgt ttataattgt attaaagagt ataaaaataa 12001 attagcgagc tcaggtcttg aagagttgga aagaagcata ggtaatgcag ttagtaaaga 12061 ggattatgtt ttaagggaat atattaacca atacaatatt tcaaaagaga gtattcttat 12121 ttattataaa aaatacagta agcctatata cacttcatta aaaagagatt ataaagaaga 12181 tgattatttt gttgtattaa aatatagagg taacaactat tactgtgggg agctcagtta 12241 tcatccaatt gaatataaat caccattaac taataaagat tttaaaataa taactgaaat 12301 gattaatagt aaagtcaatg aaatagataa tatgttcata ggcactaagg ttagagttga 12361 taaactaaca agggaaatta aaaagttatg taatgaagat aatcaattaa gaagatggat 12421 aatatccaaa gaaggacaaa taaatacatt accaagagcc gataaggaga aggttattgc 12481 taaattaaaa gaatataatg aggctccaga aatatttaaa agtaatgata aatcattaaa 12541 aatcattact gacttaaaca gtatagtaaa taaaaggaat gttcaaatta ctatggataa 12601 acctgagctc gttgcagtta taaaatacaa agtataacag gatgccacct ctatgttatg 12661 ggagctccga taaaaaaatt attctctgtg taatttttcc ataatatttg ataatctttt 12721 atttgtatat atttcatcat ggtgtgcaaa atccacaaat acaaccttct tttcattatc 12781 atccacagta aatattaaaa caaaactttt atcaatatga actcttttaa aatcttccat 12841 gggttttctt aaatttttat agtgatgtgg attttcaact atttcatcaa tttttttcag 12901 aataatttct actctttttc tatctttttt agccatcttt tttaataagt ttaataaatc 12961 cgcatctatt tttaatttat agacccaact ccctccttaa ctcatcaaca gaatcaaatt 13021 ctaccatatt gtttctttcc ataattttct ttatcttctc aacataatca ggttttaact 13081 ctggttctaa taataattct gtatattctt ctattatctt atttattgcc tcacttttat 13141 ctttaaggtt atattttgcc tttattatgt taattatttg attgttttca tctgaaatat 13201 ttactattgc cttaaccata aataccacct ttttatttat attatgtaaa tatggtatta 13261 atattatatt aatatttgta tattacttaa atgaaacatt ataaaatatt aaaataaatt 13321 cctttaaaat aatagagtaa atcgggctcc tatatatgta tagtatacaa ggggaatttt 13381 tagtaaataa ctacaaatta gactatacaa ctataaggta taaatcaaaa cgaaaataaa 13441 aaaagtatca atttatttat tatgctcaaa gttatagggc taactatcca ttatggataa 13501 gacactactt gttttaacct actaaaaact tcttccagct ttttttcctt taacatacct 13561 atcctcttta taataagctc ggtgttaatc gaaaagatta catacggttt aacaacactg 13621 ggtattattg gttttgctcc tggataaagt tcaacatcct tattatcaat taaaacattt 13681 aattcttgaa aaccgctatt aacatttgat gttatctcga ctacaataac tgtcttacta 13741 attcgattaa atttattaga tgaaactact aatgcaggtc tttttttaat agtagtcaaa 13801 tcatcaaaag gaaatggtaa aagaacaata tctccctgtt taaagtccat attaaccctt 13861 cctaataatc atcccaatat tcatcttctt tgctattcca atccttagct aaaatgggct 13921 gaaccatctt taaaacccat ttatcttctt tggaaagttt ctcttttttt acatttttgg 13981 tgggcatttc aatagtggta gtcatatact tcacctgtat aatcatttgt ttaatattat 14041 taataaattt aactattata cataatatgt taaaaatcag aaaataaagt aaccaatata 14101 aaacattgcc ttaggtttca taaggcaata tttggtaaaa aatagctgaa cttataacat 14161 tctatttttt atataccttc aacaattact ttttttcctt ttctctttct ttttattttt 14221 cctaaacttt ccagtttgga aagaatatta taaagctcac gagttgttaa atttgtataa 14281 gtcagcaggt ctgatataca aacatccttt ccattgctaa ttatatttaa aatagtatct 14341 tcaatattgt ttttattatc atttgcacta ataacattca aagagttatg aaaactttct 14401 aattcgttta atttattctc aatttctaca atcctattac atacatcgtc aatattatct 14461 ttacataacc ttaactcgtt aagctcgttt ttaagtaaat taatattatt tcttacttct 14521 ccaatagttt tcagtatgac aagtttttca ttgtttaatt catcaacttt atgttctaat 14581 ttatctattc ctaaccactt cttaatcttt ccacgcataa tataaccaaa tatgttttat 14641 tataatagta ccattatgca tccctaaaat tatgttaaaa atctatcctg ttatcttatg 14701 cacctaacat aaccctatgg tatatattgg tgttaaaact gctctgtcta tataataata 14761 tataaacata caattgtgaa cggtgaattg gattattttt ataaaaagat tgtaaatatt 14821 atagatatta tagatattgt aaagattgta aatattgtaa atagcagtaa tataaacagc 14881 ccaaaaataa ttatactatt tttatatgat tgagtaaagt cattaaatca //