LOCUS NC_015421 10798 bp DNA circular BCT 25-APR-2011 DEFINITION Lactobacillus buchneri NRRL B-30929 plasmid pLBUC03, complete sequence. ACCESSION NC_015421 VERSION NC_015421.1 GI:330370666 DBLINK Project: 66205 KEYWORDS . SOURCE Lactobacillus buchneri NRRL B-30929 ORGANISM Lactobacillus buchneri NRRL B-30929 Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae; Lactobacillus. REFERENCE 1 (bases 1 to 10798) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Chertkov,O., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Mikhailova,N., Pagani,I., Leathers,T.D., Mills,D., Liu,S. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Complete sequence of plasmid3 of Lactobacillus buchneri NRRL B-30929 JOURNAL Unpublished REFERENCE 2 (bases 1 to 10798) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (22-APR-2011) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 10798) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Chertkov,O., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Mikhailova,N., Pagani,I., Leathers,T.D., Mills,D., Liu,S. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Direct Submission JOURNAL Submitted (12-APR-2011) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002655. URL -- http://www.jgi.doe.gov JGI Project ID: 4086509 Source DNA and organism available from Siqing Liu (Siqing.Liu@ars.usda.gov) Contacts: Siqing Liu (Siqing.Liu@ars.usda.gov) Tanja Woyke (microbe@cuba.jgi-psf.org) Annotation done by JGI-ORNL and JGI-PGF Finishing done by JGI-LANL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Lactobacillus buchneri NRRL B-30929 collection_date :: Missing lat_lon :: Missing depth :: Missing alt_elev :: Missing country :: Missing environment :: Host, Intestinal microflora num_replicons :: 4 ref_biomaterial :: NRRL B-30929 biotic_relationship :: Free living trophic_level :: Missing rel_to_oxygen :: Facultative isol_growth_condt :: Missing sequencing_meth :: WGS assembly :: Newbler v. 2.3 (pre-release) finishing_strategy :: Finished GOLD Stamp ID :: Gi02021 Funding Program :: DOEM 2007 Isolation Site :: Fuel ethanol production facility Cell Shape :: Rod-shaped Motility :: Nonmotile Temperature Range :: Mesophile Gram Staining :: Gram+ Diseases :: None Phenotypes :: Probiotic ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Current Finishing Status :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: Sanger/454/Illumina ##Genome-Assembly-Data-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..10798 /organism="Lactobacillus buchneri NRRL B-30929" /mol_type="genomic DNA" /strain="NRRL B-30929" /db_xref="taxon:511437" /plasmid="pLBUC03" gene 494..1597 /locus_tag="Lbuc_2447" /db_xref="GeneID:10447587" CDS 494..1597 /locus_tag="Lbuc_2447" /inference="protein motif:PFAM:PF06970" /note="PFAM: Replication initiator A, N-terminal; KEGG: lbr:LVIS_A09 hypothetical protein" /codon_start=1 /transl_table=11 /product="replication initiator A domain protein" /protein_id="YP_004385943.1" /db_xref="GI:330370667" /db_xref="InterPro:IPR010724" /db_xref="GeneID:10447587" /translation="MDQTNFKFYQADNVYGALFFQFPKVLMYSQQYRGLSDAAKLAYM VLKDRLEYSLRNHWIDEEGHVYFIFTNQELKDLFDCSNDKLAAVKKDLERAGLLYQKA MHFNPKTGKNEPNRLYLAELDMQSTDVYLRGEYAQKEPQTLATSENPKIGRSRETAQT LATSENPKIGHSRKFVDDNPQTLATSENPKIGHDLYKDFKNIDTNRYNIDTQKLDFST AHFSPEEIQKQNQDLVNHANDFLTDEDSGLPVFLEPEAVQLLSFWCRTPQQMRRFIGI ILNAKYRVEKDHQDIGVLIPLDDEELKPLMTKALRRYFNALRSNEKHIKNVENYLYGT MQNLFSIYWNKKANAKYQAEHSKTSHQKAALFN" misc_feature 545..769 /locus_tag="Lbuc_2447" /note="Replication initiator protein A (RepA) N-terminus; Region: RepA_N; pfam06970" /db_xref="CDD:148536" gene 1885..2094 /locus_tag="Lbuc_2448" /db_xref="GeneID:10447573" CDS 1885..2094 /locus_tag="Lbuc_2448" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004385944.1" /db_xref="GI:330370668" /db_xref="GeneID:10447573" /translation="MSKKIWIVIFRYLLAIGLFLAYSALFDNQDHDLIRSTLVFAICL GLAIIPFRSCPRSMIFDILLCKKRV" sig_peptide 1885..1956 /locus_tag="Lbuc_2448" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probability 0.671) with cleavage site probability 0.585 at residue 24" gene 2098..2885 /locus_tag="Lbuc_2449" /db_xref="GeneID:10447574" CDS join(2098..2364,2364..2885) /locus_tag="Lbuc_2449" /inference="similar to AA sequence:KEGG:lhv_1988" /ribosomal_slippage /note="manually curated; KEGG: lhe:lhv_1988 putative transposase" /codon_start=1 /transl_table=11 /product="transposase" /protein_id="YP_004385945.1" /db_xref="GI:330370669" /db_xref="GeneID:10447574" /translation="MKNYPSNITRQQFELIRPALENFRKRTKPRKYDLYEVFCAVLYV LKTGCQWRQVPGDFPEWRSVYNYYKIWSTKAEPTADSLLEQVLKKIVIARRTYQGRSA LTSFIIVDAQSVKNTATAENKGYDAGKKISGIKRHLAVDINGFPQAIHMTRANVSDRD GASAMIALHAMHLRQVQNVLVDGGYSGVNFQLDVASNLNATVQVAKRNELHRFEVMPQ RWVVERSFSWLENCRRLWKNCERQLTTSLQMVVLAFLALLLKRF" misc_feature join(2107..2364,2364..2489) /locus_tag="Lbuc_2449" /note="Transposase and inactivated derivatives [DNA replication, recombination, and repair]; Region: COG3293" /db_xref="CDD:33102" misc_feature 2406..2873 /locus_tag="Lbuc_2449" /note="Transposase DDE domain; Region: DDE_Tnp_1; pfam01609" /db_xref="CDD:144990" misc_feature 2499..2879 /locus_tag="Lbuc_2449" /note="Transposase and inactivated derivatives [DNA replication, recombination, and repair]; Region: COG3293" /db_xref="CDD:33102" gene complement(3283..3648) /locus_tag="Lbuc_2450" /db_xref="GeneID:10447575" CDS complement(3283..3648) /locus_tag="Lbuc_2450" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004385946.1" /db_xref="GI:330370670" /db_xref="GeneID:10447575" /translation="MDLDFKTNKYELFDDWHQNKTKQEFTQKLQQQAQIEKTHLPKLL SREDLKIRWQMNSRQSVHQVASKPDFPQPVFAFNHGKTPLYLETEIQIFEINHPWVIT PSARLAYSHWILRNVIDQS" gene complement(3661..4248) /locus_tag="Lbuc_2451" /db_xref="GeneID:10447576" CDS complement(3661..4248) /locus_tag="Lbuc_2451" /inference="protein motif:PFAM:PF00589" /note="PFAM: Integrase, catalytic core, phage; KEGG: lme:LEUM_A08 integrase" /codon_start=1 /transl_table=11 /product="integrase family protein" /protein_id="YP_004385947.1" /db_xref="GI:330370671" /db_xref="GO:0003677" /db_xref="InterPro:IPR002104" /db_xref="GeneID:10447576" /translation="MQQVVLPIKDSNVLKEVQDTLLNNFKAGRRNYTVFQVGKATLLR VSDVMRLKQADIFNPDGSIKQNAFIHDRKTGKPNTLYLKPVQTELLLYRQWLLDHRLA TEWLFPSIQHPERHITEKQFYKIMSKVGDLLGINYLGTHTMRKTGAYRVYTQSNYNIG LVMNLLNHSSEAMTLAYLGLDQASTETMLDQIDFG" misc_feature complement(3667..4242) /locus_tag="Lbuc_2451" /note="INT_SG3, DNA breaking-rejoining enzymes, integrase/recombinases subgroup 3, catalytic domain. The CD contains various predicted bacterial and phage integrase/recombinase sequences for which not much experimental characterization is available; Region: INT_SG3_C; cd01186" /db_xref="CDD:29507" misc_feature complement(order(3718..3720,3745..3747,3817..3819, 3826..3828,4030..4032,4117..4119)) /locus_tag="Lbuc_2451" /note="Int/Topo IB signature motif; other site" /db_xref="CDD:29507" gene 4325..4603 /locus_tag="Lbuc_2452" /db_xref="GeneID:10447577" CDS 4325..4603 /locus_tag="Lbuc_2452" /inference="protein motif:TFAM:TIGR01552" /note="KEGG: lbr:LVIS_B20 antitoxin of toxin-antitoxin stability system; TIGRFAM: Prevent-host-death protein; PFAM: Prevent-host-death protein" /codon_start=1 /transl_table=11 /product="prevent-host-death family protein" /protein_id="YP_004385948.1" /db_xref="GI:330370672" /db_xref="InterPro:IPR006442" /db_xref="GeneID:10447577" /translation="MTLALTQSDFRANLKKYLDQVNDEDETVYIARSNSRAVAIVSQE KMDWLERALKAKEGSLEYAIARDQLIKRHVLPDDEIVESDDDYWGQFK" misc_feature 4334..4543 /locus_tag="Lbuc_2452" /note="Phd_YefM; Region: PhdYeFM; cl09153" /db_xref="CDD:195806" gene 4603..4959 /locus_tag="Lbuc_2453" /db_xref="GeneID:10447578" CDS 4603..4959 /locus_tag="Lbuc_2453" /inference="protein motif:TFAM:TIGR02385" /note="KEGG: lbr:LVIS_B21 hypothetical protein; TIGRFAM: Addiction module toxin, RelE/StbE; PFAM: Plasmid stabilisation system" /codon_start=1 /transl_table=11 /product="addiction module toxin, RelE/StbE family" /protein_id="YP_004385949.1" /db_xref="GI:330370673" /db_xref="InterPro:IPR007712" /db_xref="InterPro:IPR012753" /db_xref="GeneID:10447578" /translation="MKKLRFKPRATFNADLKRLASLDKSIIDEVRAAIDLLLEQQQLP PEFEDHELNRRMSGYNEFHLRDTPKNKTPSETNDVLVVYTIDKDELVLIGIRVGSHDR LFPGQNRSKRYRKNDE" misc_feature 4603..4914 /locus_tag="Lbuc_2453" /note="Plasmid stabilisation system protein; Region: Plasmid_stabil; cl11422" /db_xref="CDD:196226" gene complement(5070..5486) /locus_tag="Lbuc_2454" /db_xref="GeneID:10447579" CDS complement(5070..5486) /locus_tag="Lbuc_2454" /EC_number="3.1.3.48" /inference="protein motif:TFAM:TIGR02691" /note="KEGG: lfe:LAF_1155 arsenate reductase; PFAM: Protein-tyrosine phosphatase, low molecular weight; manually curated; TIGRFAM: Arsenate reductase StaphA (thioredoxin); SMART: Protein-tyrosine phosphatase, low molecular weight" /codon_start=1 /transl_table=11 /product="protein tyrosine phosphatase" /protein_id="YP_004385950.1" /db_xref="GI:330370674" /db_xref="GO:0004725" /db_xref="GO:0030612" /db_xref="InterPro:IPR014064" /db_xref="InterPro:IPR017867" /db_xref="GeneID:10447579" /translation="MKKIYFLCTGNSCRSQMAEGYAKELFPSDKFEIRSAGVEKHGLN KNAVQVMAEDGVDISQQYSKLIDLNYFNSANLIVTLCGDARDKCPVIPPQATSIHWPL PDPAKATGTEKEKLQAFKKVRNKIKDNLFELVQNYK" misc_feature complement(5103..5483) /locus_tag="Lbuc_2454" /note="Low molecular weight phosphatase family; Region: LMWPc; cd00115" /db_xref="CDD:29014" misc_feature complement(order(5442..5450,5454..5459,5463..5465)) /locus_tag="Lbuc_2454" /note="Active site [active]" /db_xref="CDD:29014" gene 5587..6507 /locus_tag="Lbuc_2455" /db_xref="GeneID:10447580" CDS 5587..6507 /locus_tag="Lbuc_2455" /inference="protein motif:PFAM:PF00665" /note="PFAM: Integrase, catalytic core; KEGG: lcr:LCRIS_00542 transposase" /codon_start=1 /transl_table=11 /product="Integrase catalytic region" /protein_id="YP_004385951.1" /db_xref="GI:330370675" /db_xref="GO:0003677" /db_xref="InterPro:IPR001584" /db_xref="GeneID:10447580" /translation="MGTSTLSRFQRGALAQLVNEGNKSYQVMADALGVAKATISYELD RVKPYDPELAQQDADRKRRNCGRRSMLTAALATLITNHLRLTWSPETIAAAYNLSTAS IYNWLNRGWLPFKLTDLPNRNVRQHRVSENRGKFTSGTSIEQRPTTVNQRLAFGHWEV DTVLSSRSESRSCLVTFVERKTRLLWAIKAPNRTAKALNTAFGKFMGAFGPQVKSITV DHGKEFANYQALEQDYQIKVYFCHPYSPWERGSNEYFNRRLRWFFPKKTNFSQVTTDE ILAALELINQRPLKIHHQQTAIERFRACSD" misc_feature 5587..6489 /locus_tag="Lbuc_2455" /note="Transposase and inactivated derivatives, IS30 family [DNA replication, recombination, and repair]; Region: Tra8; COG2826" /db_xref="CDD:32654" misc_feature 6049..6366 /locus_tag="Lbuc_2455" /note="Integrase core domain; Region: rve; cl01316" /db_xref="CDD:194099" gene complement(6535..8430) /locus_tag="Lbuc_2456" /pseudo /db_xref="GeneID:10447581" gene complement(7536..8323) /locus_tag="Lbuc_2457" /db_xref="GeneID:10447582" CDS complement(join(7536..8057,8057..8323)) /locus_tag="Lbuc_2457" /inference="similar to AA sequence:KEGG:lhv_1988" /ribosomal_slippage /note="manually curated; KEGG: lhe:lhv_1988 putative transposase" /codon_start=1 /transl_table=11 /product="transposase" /protein_id="YP_004385952.1" /db_xref="GI:330370676" /db_xref="GeneID:10447582" /translation="MKNYPSNITRQQFELIRPALENFRKRTKPRKYDLYEVFCAVLYV LKTGCQWRQVPGDFPEWRSVYNYYKIWSTKAEPTADSLLEQVLKKIVIARRTYQGRSA LTSFIIVDAQSVKNTATAENKGYDAGKKISGIKRHLAVDINGFPQAIHMTRANVSDRD GASAMIALHAMHLRQVQNVLVDGGYSGVNFQLDVASNLNATVQVAKRNELHRFEVMPQ RWVVERSFSWLENCRRLWKNCERQLTTSLQMVVLAFLALLLKRF" misc_feature complement(join(7932..8057,8057..8314)) /locus_tag="Lbuc_2457" /note="Transposase and inactivated derivatives [DNA replication, recombination, and repair]; Region: COG3293" /db_xref="CDD:33102" misc_feature complement(7548..8015) /locus_tag="Lbuc_2457" /note="Transposase DDE domain; Region: DDE_Tnp_1; pfam01609" /db_xref="CDD:144990" misc_feature complement(7542..7922) /locus_tag="Lbuc_2457" /note="Transposase and inactivated derivatives [DNA replication, recombination, and repair]; Region: COG3293" /db_xref="CDD:33102" gene complement(8481..8798) /locus_tag="Lbuc_2458" /db_xref="GeneID:10447583" CDS complement(8481..8798) /locus_tag="Lbuc_2458" /inference="protein motif:PFAM:PF01022" /note="KEGG: lfe:LAF_1157 arsenical resistance operon repressor; PFAM: HTH transcriptional regulator, ArsR; SMART: HTH transcriptional regulator, ArsR" /codon_start=1 /transl_table=11 /product="transcriptional regulator, ArsR family" /protein_id="YP_004385953.1" /db_xref="GI:330370677" /db_xref="GO:0003700" /db_xref="InterPro:IPR001845" /db_xref="GeneID:10447583" /translation="MHYEYKDLASLLKVVAEPNRLKILEMLSCGEMCGCDILAHFNFT QPTLSHHMSILEKSGLVNVRKDTKWHYYSLNSNKKEGLISELTQILSKTDHCICKDSH SNC" misc_feature complement(8562..8765) /locus_tag="Lbuc_2458" /note="Arsenical Resistance Operon Repressor and similar prokaryotic, metal regulated homodimeric repressors. ARSR subfamily of helix-turn-helix bacterial transcription regulatory proteins (winged helix topology). Includes several proteins that appear to...; Region: HTH_ARSR; cd00090" /db_xref="CDD:28974" misc_feature complement(order(8562..8564,8622..8624,8712..8714, 8721..8726,8733..8738,8745..8747,8754..8756,8760..8765)) /locus_tag="Lbuc_2458" /note="dimerization interface [polypeptide binding]; other site" /db_xref="CDD:28974" misc_feature complement(order(8583..8591,8604..8612,8628..8633, 8637..8642,8649..8654,8658..8669,8694..8702,8739..8747, 8757..8762)) /locus_tag="Lbuc_2458" /note="putative DNA binding site [nucleotide binding]; other site" /db_xref="CDD:28974" misc_feature complement(order(8562..8564,8691..8693,8700..8702)) /locus_tag="Lbuc_2458" /note="putative Zn2+ binding site [ion binding]; other site" /db_xref="CDD:28974" gene 9305..9652 /locus_tag="Lbuc_2459" /db_xref="GeneID:10447584" CDS 9305..9652 /locus_tag="Lbuc_2459" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004385954.1" /db_xref="GI:330370678" /db_xref="GeneID:10447584" /translation="MLKKIGYVIALLLGFFLIVWLAIYWQHRTYFTGAVRTYMVAQKI PKNSIKNQKIVFNWVNMGQWEEDVSIRHQHHTYQYIYNMSWQDKRVSLSIYDHREGVK DTLAPYPPLEYDR" sig_peptide 9305..9370 /locus_tag="Lbuc_2459" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probability 0.677) with cleavage site probability 0.669 at residue 22" gene 9711..9950 /locus_tag="Lbuc_2460" /db_xref="GeneID:10447585" CDS 9711..9950 /locus_tag="Lbuc_2460" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004385955.1" /db_xref="GI:330370679" /db_xref="GeneID:10447585" /translation="MSLAELGSFALLISLVYWWFLMWHSFKLTRKTLVILNTVGTALM TILTWPITYGMLLIPILIIYAVSTFIIFKLPLAAK" gene complement(10061..10627) /locus_tag="Lbuc_2461" /db_xref="GeneID:10447586" CDS complement(10061..10627) /locus_tag="Lbuc_2461" /inference="similar to AA sequence:KEGG:LVIS_A10" /note="KEGG: lbr:LVIS_A10 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004385956.1" /db_xref="GI:330370680" /db_xref="GeneID:10447586" /translation="MGKTIRQLSEEFKLSKQAINQRINSINGFRSKHTYKVKNHLEID NQGVKMLADFDKQKRQERQPNRQDENDKDKNINHILLKQLDVKDQQIENLQQALDQQQ KLQIATVSENHRLKEHVRKLSGLLDSSSATQQQKSNDKDDVLSNNVNTRHSKDKNDKQ NENRVAEYNNRQKKVSKNKVNNSWWRFW" ORIGIN 1 ctagactata gttaatttaa caatcaaata ttaaaagatc aggctaatac tataaaagag 61 atcccaatcc attcgggatt gagatctctt ttatagtatt agcaagtagc tcataccagc 121 tacttagatt cagtcgattt tgacttcccg gaaaaaatcg actgaatatt gcttttaact 181 ttgtaaggta attataccgc agaccagccc atgaggacaa ggttcggata gttaaaaaac 241 aaaattgaaa ccaatggact aagtagctag tagaagctac ctagtccatt tttattttat 301 tgttcaaatt aaaaaagttg ccgaatgggc aaccaagcaa gatccatgct gataaattac 361 aaagttctca caaccaatga atactttgta attcagttag agcatatcag atttgtattt 421 taaaacaaat cagatgtttt agcaactctc ttttgagcca gcatgggtcg gacatagaaa 481 gggagttttt ataatggatc aaacaaattt caagttttat caagctgata acgtttatgg 541 ggccttgttc ttccagtttc caaaagtatt gatgtatagc cagcaatatc gaggattaag 601 tgatgccgct aagctagctt acatggtact aaaagaccgg ctagagtatt ctttacgcaa 661 tcactggatt gatgaggaag ggcacgtcta ctttattttt accaatcaag aacttaaaga 721 tttattcgac tgctcaaatg ataagttagc agccgtgaaa aaagacttgg aacgtgcagg 781 actactttat caaaaagcca tgcattttaa tcccaaaaca gggaaaaatg aacccaatcg 841 actttacctg gccgaactag atatgcagtc aactgacgtc tatttacgcg gtgaatatgc 901 tcaaaaagag ccgcaaaccc ttgctacgag cgaaaatccg aaaatcggac gttcgcggga 961 gaccgctcaa acccttgcta cgagcgaaaa tccgaaaatc ggacattcgc gaaagttcgt 1021 tgacgacaac ccgcaaaccc ttgctacgag cgaaaatccg aaaatcggac acgatctata 1081 taaagacttt aaaaacatag atactaatag atacaatata gatactcaaa agttggactt 1141 ttccacagcc catttttcac cagaagaaat tcaaaagcaa aaccaggatt tggtgaacca 1201 tgctaatgac ttcttaactg atgaagacag tggcttaccg gttttcttag aacccgaagc 1261 cgtgcaatta ctaagtttct ggtgccgcac tccgcaacaa atgcggcgct tcattggcat 1321 tatcttaaat gctaagtacc gagttgaaaa agatcatcaa gatattgggg ttctaatccc 1381 gcttgacgac gaagaactaa aacctttgat gactaaagcc ttgagacgct actttaacgc 1441 cctaagaagc aatgagaagc atatcaagaa cgtcgagaac tacttgtacg gcaccatgca 1501 aaatctattt agcatttact ggaacaaaaa agccaacgct aaatatcaag ctgaacattc 1561 taaaacaagt catcagaaag cagccctctt taattagctc taaaatcgtt tctcagccgt 1621 tttagccatg gactatatat ttatattaaa atagcgattg aattgcctat acaaacaaac 1681 agcgggcaat tttagcaggc ttagccaact tgaatcagcg ggctaatcat tttaaatgat 1741 cactgcttga ctagtttagc ttaaaaatgc gcttagcagt ctccaggggg aactatgctt 1801 tttttagcta catgaattag caagaatagt tttttaatat tgattaaact gagtatgagc 1861 aacttgttct aggaggcggg gtttatgagc aaaaaaattt ggattgttat ttttcgttat 1921 ttattagcga ttggcttatt tttagcctat agtgcattat ttgataatca agatcatgat 1981 ttgattagat cgactcttgt ttttgctata tgtcttggcc ttgctattat tccatttaga 2041 agctgtccaa gatctatgat ttttgatata cttctatgta aaaagcgagt ttaagctatg 2101 aaaaattatc ccagcaatat tactcggcaa caatttgaat taattcgacc agctttagaa 2161 aattttcgta agcgaactaa gcctcgaaaa tatgacctct acgaggtctt ctgtgcggtc 2221 ctatatgtct tgaaaaccgg ttgccaatgg cgtcaagtcc ccggtgattt cccagaatgg 2281 cggtcagttt acaactatta caaaatttgg tcaactaaag ctgagcctac ggctgattct 2341 ttattggaac aagttttaaa aaaattgtca ttgctcggcg aacttaccaa ggacgttcag 2401 ctttaacttc ctttattatc gttgacgctc agagcgtcaa aaacaccgct actgctgaaa 2461 acaaaggtta cgacgctggt aagaaaatct cggggattaa gcgccatctg gcagttgata 2521 tcaatggctt tccgcaggcc attcacatga cgcgagcgaa cgtctctgat cgagacgggg 2581 ccagtgcaat gatcgcttta catgccatgc atttacgcca ggttcaaaat gtcttagttg 2641 atggtggtta ttcaggcgtt aattttcagc tcgatgtagc cagtaattta aacgcaaccg 2701 tgcaggttgc gaagcgcaat gagttgcatc gattcgaagt catgccccaa cgttgggtag 2761 tcgaacgatc ttttagttgg ctagagaatt gtcggcgact ttggaaaaat tgtgagcgtc 2821 aattaaccac cagtctgcaa atggtcgttt tagcgttttt agcactatta cttaagagat 2881 tttagacagc ttcttagcca taagaccaaa agcaaaaaat agtttaaggg atatatataa 2941 ttgatattct tgtaaaaaga tactcatctg gccatttttc agtgtatctc atgttgttaa 3001 ttgctttaat tatctagtta aaattaataa cgaatcaatg agagctatta tggctaaagg 3061 gtgtgtttaa agcaggccta gttaatgaaa atagataaag aacactaaaa ttagtggtgg 3121 gtaatcagca gaccgagtga aacgaggtca atgcgcactt acacccttga caagtgtcaa 3181 gggtagattt aatacccatt tcgatgggta ttatttgtat tagaaggcta gcgccaacta 3241 taaaaaaact agagtaaacc ctcaaaaacc tagagctgaa tatcaagatt gatcaatcac 3301 attgcgaagg atccaatgac tataagcaag acgagcactg ggagttatca cccaggggtg 3361 attaatttca aaaatttgaa tttcagtttc taagtaaagc ggcgtcttac catgattaaa 3421 agcaaaaacg ggttgtggaa aatcaggctt actagcaact tgatgaacac tttgacgaga 3481 gttcatctgc caccgaattt ttagatcttc acgcgacaat aattttggca ggtgtgtttt 3541 ttcaatttga gcttgttgct gcaacttttg tgtaaattct tgtttagtct tgttttggtg 3601 ccaatcatca aacagctcat atttgttggt tttaaaatct aaatccataa ggacaacctc 3661 ctaaccaaaa tcaatctgat ccagcatcgt ttcggtacta gcctggtcta agcctaaata 3721 agctaaagtc atcgcctcac tggaatgatt gagcagattc atgactaagc caatattata 3781 atttgattgc gtgtagacgc gataagcccc ggttttgcgc attgtatggg tccctagata 3841 attaattcct aacagatcgc caaccttact cataattttg tagaactgtt tttccgtgat 3901 atggcgctcg gggtgttgaa tggaaggaaa gagccattca gtggctagtc tatgatcaag 3961 tagccattga cggtacaata agagctctgt ttgaactggt ttaagataca aggtattagg 4021 cttacccgtt tttcggtcgt gaataaacgc attttgttta atagaaccgt ccggattaaa 4081 aatatcggcc tgttttaagc gcatgacatc actgactcgc aacagtgtcg ctttgccaac 4141 ttgaaaaact gtatagttac gccggccagc tttaaagttg ttgagtaagg tatcttgaac 4201 ctctttgaga acgtttgaat ctttgatggg taagacaact tgttgcatag ttttagctcc 4261 ttaaaaaatt gatttttagt acaaattaaa gtacaatatt aagtacaatg aaagggtggt 4321 aaatatgaca ttagcactaa cacagagcga ttttcgcgct aacctaaaaa aatatttaga 4381 tcaagttaat gacgaagacg aaaccgttta tattgctcgt tcaaatagtc gcgcagtagc 4441 catcgtttca caagaaaaaa tggactggct agaaagagca ttaaaagcta aagaaggttc 4501 gttagaatat gcaattgcac gtgatcagtt aattaaacgc catgttttac ctgacgatga 4561 aattgttgaa tcagatgatg attattgggg tcagtttaaa taatgaaaaa actaagattt 4621 aaaccacgtg caacctttaa tgctgatcta aaacggttag ccagtttaga caaatctatt 4681 attgatgaag ttcgagcagc cattgacctg ttgcttgagc aacaacaatt accaccagaa 4741 tttgaagatc atgagcttaa tcggcgaatg agcgggtata atgaatttca cttacgagat 4801 accccgaaaa acaaaacacc aagcgaaact aacgatgtcc tggtggttta tacgattgat 4861 aaagatgaac tagtcttaat tggaattcga gtcggatcgc atgatcgttt atttcctggt 4921 caaaatcgtt ctaaaaggta tcgaaaaaat gacgaataga agaagtcatt tttatagtat 4981 gaaaataaat aacccccaat atctatatta tagatattgg gggttattta tttcaatgtt 5041 tttgaggggc gatttgtaag aaatggaccc tatttataat tttgtacaag ttcgaataga 5101 ttatctttta ttttgtttcg aactttttta aaggcttgta gtttttcttt ttctgttcca 5161 gtggcttttg cagggtcggg taatggccaa tgaatacttg tagcttgtgg tggaataact 5221 ggacatttat ctcgtgcatc accgcataaa gtaacaatta gattagctga attgaagtaa 5281 tttaaatcaa tcagttttga atactgctga ctaatatcga caccatcttc tgccataact 5341 tgtactgcat ttttgtttaa accatgcttt tctacgcccg cacttctaat ttcaaattta 5401 tcggaaggaa aaagttcttt tgcgtatcct tcggccattt ggcttcgaca tgaattcccg 5461 gtacagagaa aatagatttt tttcatttgg caaattctat aattaatccg aacagaaatt 5521 aaaaagccca aagcaatcac ttacaatggt agtagctaat tccaaccaat atagggagtg 5581 attactttgg gtacatctac tttatcacgt tttcaacgtg gcgcactagc acaactggtc 5641 aatgagggga ataaatctta ccaagtaatg gctgacgcct taggcgtcgc caaagctacg 5701 attagctatg agttggaccg ggttaaacct tatgatccag aattagctca gcaagatgca 5761 gatcgcaaaa ggcggaattg cggtcgtcgt tcgatgctga cggcagcatt agcgacttta 5821 attaccaatc acttacgatt aacctggtca ccagaaacca ttgcggccgc ttataacttg 5881 agcactgcgt caatttataa ttggcttaat cgtggctggc tccccttcaa attgactgat 5941 ctacccaatc ggaatgtccg ccagcaccga gtgagcgaaa atcgtgggaa atttacaagt 6001 gggacttcca tcgaacaacg gccaacaact gttaatcaac ggttagcttt tggtcattgg 6061 gaagtagata cggtgctttc tagtcgaagt gagtcacgat catgtctggt tacattcgta 6121 gaacgtaaga cccgacttct atgggccatc aaagccccta atagaacggc taaggctcta 6181 aacaccgcct ttggcaagtt tatgggggcc ttcggtcccc aagtaaaatc cattactgtt 6241 gatcatggta aagagtttgc caattatcag gccttagaac aggattatca gatcaaagtt 6301 tatttttgcc atccatattc accatgggag cgaggttcca atgaatattt taatagacgg 6361 ttacgctggt tcttcccgaa aaagaccaat tttagccaag taacgactga tgagatccta 6421 gcagcacttg aactaattaa tcaacgacca ttaaaaatac atcatcaaca gactgccatt 6481 gaaagattcc gggcttgttc ggattaaact tgtaatttgc catttggtta ctccttaatc 6541 ataatatttt cttttaatcc atagtgctac atcaactaat aagatcatta ccggtacttc 6601 aaccattggg ccaataacgg cagcaaatgc ttctccagaa ttcaagccaa atatagcgac 6661 tgttactgca acagctaatt caaagttatt acttgcagcg gtgaaagatt gtgtcgcaga 6721 aattgaataa ctagttttag tctttttcgc aatccaaaag gttatgaaga acattaagac 6781 aaaatatagt aataaaggaa cagcaattct tattgcgtcc attggtagtt gaactacttt 6841 tgcacccttg acagaaaaca tcactactat tgtaaagagt aaagcccaag tagtaattct 6901 gcttattttt ggtacatatt tatcttcaaa ccattcttta ccctttgtgc taatgataaa 6961 gtaacgagaa ccaatgccca aaatgaaagg aataccaaga taaataaaaa cacttttagc 7021 aatttcactc attgtaatat taattgtctg tgtttgtagc ccaaagaatt ttggtaagac 7081 actaataaag aagtatgcat aaacggaata tagcaaaatc tgaaaaattg agttaaaagc 7141 tactagacca gcagcataat cgttattacc ctgcgctaat tcattccaca caataaccat 7201 agcaatacac cgtgctaaac cgatcatgat taaaccaatc atatagtttg gataattgtg 7261 tagaaagata acagcaagag caaacattaa aatagggcca acgatccaat tttgaactaa 7321 tgaaaaagca agttcctttt tgtccttaaa tacatcaccc attttgccat aattaacttt 7381 ggttagtggt ggatatagca ttaatattaa gcctattgct attggaattg atgttgtacc 7441 gatagaccaa ctattaatta ttttagggag cgatggaaaa gctaaaccgc taataattcc 7501 cagtgccata gctaaaaata cccataagaa gctgtctaaa atctcttaag taatagtgct 7561 aaaaacgcta aaacgaccat ttgcagactg gtggttaatt gacgctcaca atttttccaa 7621 agtcgccgac aattctctag ccaactaaaa gatcgttcga ctacccaacg ttggggcatg 7681 acttcgaatc gatgcaactc attgcgcttc gcaacctgca cggttgcgtt taaattactg 7741 gctacatcga gctgaaaatt aacgcctgaa taaccaccat caactaagac attttgaacc 7801 tggcgtaaat gcatggcatg taaagcgatc attgcactgg ccccgtctcg atcagagacg 7861 ttcgctcgcg tcatgtgaat ggcctgcgga aagccattga tatcaactgc cagatggcgc 7921 ttaatccccg agattttctt accagcgtcg taacctttgt tttcagcagt agcggtgttt 7981 ttgacgctct gagcgtcaac gataataaag gaagttaaag ctgaacgtcc ttggtaagtt 8041 cgccgagcaa tgacaatttt tttaaaactt gttccaataa agaatcagcc gtaggctcag 8101 ctttagttga ccaaattttg taatagttgt aaactgaccg ccattctggg aaatcaccgg 8161 ggacttgacg ccattggcaa ccggttttca agacatatag gaccgcacag aagacctcgt 8221 agaggtcata ttttcgaggc ttagttcgct tacgaaaatt ttctaaagct ggtcgaatta 8281 attcaaattg ttgccgagta atattgctgg gataattttt catagcttaa actcgctttt 8341 tacatagaag tatatcaaaa atcatagatc ttggacagct tctaaggtca tataacggtc 8401 catgaatgat aaatttttag aagtacgcat aatagccctc catatagata tttatctatg 8461 tataatgcaa aataaaaaag ctaacagttt gaatgtgaat ccttacatat gcaatggtca 8521 gttttagata gtatttgtgt taattcacta atcagtcctt cttttttgtt tgaatttaaa 8581 gaataatagt gccacttagt atcttttctt acgttaacta atccactttt ttccagaata 8641 ctcatatggt gggatagagt cggttgtgta aagttaaagt gtgcaagtat atcacaccca 8701 cacatctcac cacaggacag catttctaaa atcttaagac ggttaggttc agcaaccacc 8761 tttaaaagtg atgccaaatc tttatactca tagtgcatca gtaaattcct ccacattaaa 8821 catagacgat tatctatatt gtatcacatg aataatgaaa aagattgccg ttcggacgaa 8881 attagatttt ttactattat tacaaaacca gcagtattaa tgtgttattc gccaaagcca 8941 accatgtttt tcttcatttg ccgatttagc tgttactttt tgtggtaaga agctggttgt 9001 tttggccttg ctcggtttga atccgtttga gtgcggtcag attttcctga ctgatcaggt 9061 tggtctgctt ttgaaacgtt gcgtaggcgc tttgtaaggt ctgttttaat tgggcttgcg 9121 agctggcgtt gctgctgtct aaatatgttt acttagctta ttggtggggg acaagtcata 9181 aaggaatgaa aggtggcctt atgtgggtat tcccattctc aatgacaaat gtttttttag 9241 ggacttacaa gccaagttat atttataaac taacaacaaa atgaaaaaga ggtgtggatt 9301 agttatgctt aaaaagattg ggtacgtaat agctttatta ctagggttct ttttaatagt 9361 ttggctagcc atttattggc aacatcgcac ttattttact ggagccgttc gaacgtacat 9421 ggttgcccaa aagattccaa aaaatagcat taaaaatcag aaaatagttt ttaattgggt 9481 caatatggga caatgggagg aagatgtttc aattcgccac caacaccata cctatcaata 9541 tatttataat atgtcttggc aggataaaag ggtgtcccta agtatctatg atcatcggga 9601 gggtgttaaa gatactctag ctccttatcc acccttggag tatgatcgtt aatttaattt 9661 ttgcaagctt taatatatta caaaaacaat ttataggaga taaataaaaa ttgtcattag 9721 ctgaattggg aagctttgcg ctacttattt cattggtcta ctggtggttt ttaatgtggc 9781 attcatttaa actaaccagg aaaacgttag taattttaaa tacagttggc actgccttaa 9841 tgaccatttt gacgtggccc atcacctatg gaatgttact gattccgatt ttaattattt 9901 acgctgtatc gacttttatc atctttaaat taccactggc agccaaatag tatgagaatt 9961 tttttgcttc ttataactat ccaaggcggt aggaaaatgg gctctttgtc aactatttct 10021 gggttagatt cctgattgct tgatgaaagg agtagaaata ctaccaaaaa cgccaccaac 10081 tattattgac tttgttttta gatacttttt tttgcctatt attatattca gcaactctat 10141 tttcattttg cttgtcgttt ttatccttgc tgtgacgggt attgacatta ttcgacaaga 10201 catcgtcctt gtcgtttgat ttttgctgtt gagttgcact agaagagtca agcaagccac 10261 tcaatttacg aacgtgctct tttaatcgat ggttttctga aaccgttgct atctgtaatt 10321 tttgttgctg atctaaggcc tgttgtaaat tttcaatttg ttgatcctta acatcaagtt 10381 gtttcagcaa gatatgatta atgtttttgt ccttgtcgtt ttcatcttgc cgatttggtt 10441 gtcgttcttg tcgtttttgc ttgtcaaaat cagcaagcat tttaacgcct tggttgtcta 10501 tttctagatg gtttttaact ttgtaagtgt gtttgcttcg aaagccatta atgctattaa 10561 tacgttgatt aatagcttgt tttgacaatt taaattcttc tgaaagttgc cgaatagtct 10621 tgcccaatag tttcaccgcc taattaatcc gccttaaatt ataagttaaa aacgacaaga 10681 acaccaatcg acaagtaatg tttaaacttg ttgtcgttct tgtcgttttt tcatcttaat 10741 atcaatttct tagataatct atttacaaat taaggtttac cggttagtaa gcgctatg //