LOCUS NC_015313 15063 bp DNA circular BCT 30-MAR-2011 DEFINITION Pseudonocardia dioxanivorans CB1190 plasmid pPSED03, complete sequence. ACCESSION NC_015313 VERSION NC_015313.1 GI:327309734 DBLINK Project: 65087 KEYWORDS . SOURCE Pseudonocardia dioxanivorans CB1190 ORGANISM Pseudonocardia dioxanivorans CB1190 Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; Pseudonocardineae; Pseudonocardiaceae; Pseudonocardia. REFERENCE 1 (bases 1 to 15063) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Chertkov,O., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I., Sales,C.M., Parales,R.E., Alvarez-Cohen,L., Mahendra,S. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Complete sequence of plasmid3 of Pseudonocardia dioxanivorans CB1190 JOURNAL Unpublished REFERENCE 2 (bases 1 to 15063) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (28-MAR-2011) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 15063) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Chertkov,O., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I., Sales,C.M., Parales,R.E., Alvarez-Cohen,L., Mahendra,S. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Direct Submission JOURNAL Submitted (16-MAR-2011) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002598. URL -- http://www.jgi.doe.gov JGI Project ID: 4083663 Source DNA and organism available from Shaily Mahendra (mahendra@seas.ucla.edu) Contacts: Shaily Mahendra (mahendra@seas.ucla.edu) Tanja Woyke (microbe@cuba.jgi-psf.org) Annotation done by JGI-ORNL and JGI-PGF Finishing done by JGI-LANL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Pseudonocardia dioxanivorans CB1190 collection_date :: Missing lat_lon :: 34.299366 -79.876714 depth :: Missing alt_elev :: Missing country :: USA environment :: Sludge, Fresh water num_replicons :: 4 ref_biomaterial :: ATCC 55486, DSM 44775 biotic_relationship :: Free living trophic_level :: Missing rel_to_oxygen :: Aerobe isol_growth_condt :: Missing sequencing_meth :: WGS assembly :: Newbler v. 2.3 (pre-release) finishing_strategy :: Finished GOLD Stamp ID :: Gi02043 Type Strain :: Yes Greengenes ID :: 91804 Isolation Site :: Industrial sludge contaminated with 1,4-dioxane Isolation Comments :: isolated by RE Parales, JA Romesser, for Celgene Corporation Temperature Range :: Mesophile Temperature Optimum :: 30CC Gram Staining :: Gram+ Diseases :: None ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: 454, Illumina ##Genome-Assembly-Data-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..15063 /organism="Pseudonocardia dioxanivorans CB1190" /mol_type="genomic DNA" /strain="CB1190" /isolation_source="industrial sludge contaminated with 1,4-dioxane" /db_xref="taxon:675635" /plasmid="pPSED03" /country="USA" /lat_lon="34.299366 N 79.876717 W" /collected_by="RE Parales, JA Romesser" gene complement(1620..4226) /locus_tag="Psed_7012" /db_xref="GeneID:10361801" CDS complement(1620..4226) /locus_tag="Psed_7012" /inference="protein motif:TFAM:TIGR01613" /note="TIGRFAM: DNA primase, phage/plasmid; PFAM: Phage/plasmid primase, P4, C-terminal; KEGG: afl:Aflv_0784 phage associated DNA primase; SMART: Phage/plasmid primase, P4, C-terminal" /codon_start=1 /transl_table=11 /product="P4 family phage/plasmid primase" /protein_id="YP_004336634.1" /db_xref="GI:327309735" /db_xref="InterPro:IPR006500" /db_xref="InterPro:IPR014818" /db_xref="GeneID:10361801" /translation="MSDNSMRKPPRAAPHTFNDMGNARRLVELCGDDVRHFRGTWYVW DGKRFARDQDYRVQEQAKRVPLEMRKQAYRAISASKELSHDERKAAETSARELLKFAD ASGNRLRLASMIELARTEPEITVTDADAFDSDPWAFNVQNGTIDLRTGELREHSRGDM ITRISPATYDPDARSELFDKFLARIQPDPVERRALQLCVGYTLTGFTGEAKLFTANGG GRNGKNVLLDTVAHLLGDYYRKAPAGFLTARKEDGTSASPDMADMRGARMVMASETER GDRLAESRVKELTGDRTITARFLYGDFFSFRPTFKIWLLTNYRPSVRGTDEGIWSRLA LIPFREYITPEERDPHLTDKLIGLFNGDPSDLSGVLTWAVEGARAWASNKTLALPDTW RAAAEDYRVEQDLMGAFLSDHCIFRPGEITTSGDLYAHYVWWCRQAGEHARSQRAFSI ELKQRPEYMKNKVLARKSDGRVVFDGMRGLKPNERTHLVAVAAPDESVTGSARGDNEG DDSRPALRAVGELDTRTAVGQNTPVPTQAPSTRNTPAPTQAPSVRRALTPDERPAART RVMTSSKAALSENRIPSVLHALEGPCAPLRSGREPRWSPERMPGIAFAAIVKNHNWRR PFRGQTITLDRSGAFLAAIASVDVSHGGLEHTGAMDQYPGLPGYYEVPLFPWTESESL PHPIPGIDKAAKTAWITAPHASLLQELVNAGRWPEFDILDSYTGETSVRLNAGGWSDF IKELRQDVINEYGRDSEQYTLQKRGFSRALTLMLGDGEPGKPRKWECRARRPDWTHAV YAHASAMLWRRADECLRLAPKSGPVAVQRVDEIVIPKRARAAVFAEGSKIVHDPLGLK LGSFKVKSDGEE" misc_feature complement(3666..4160) /locus_tag="Psed_7012" /note="D5 N terminal like; Region: D5_N; pfam08706" /db_xref="CDD:149685" misc_feature complement(2883..3821) /locus_tag="Psed_7012" /note="C-terminal domain; Region: primase_Cterm; TIGR01613" /db_xref="CDD:130674" gene complement(4518..5090) /locus_tag="Psed_7013" /db_xref="GeneID:10361788" CDS complement(4518..5090) /locus_tag="Psed_7013" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004336635.1" /db_xref="GI:327309736" /db_xref="GeneID:10361788" /translation="MEAKRAWHAPSEWAYHRDIADRESELWERQCGRQGCDESTGISR MTLCKRRPRGWASSSLIQSPRLLTPRSSLSWTSASVRKFSGTHMHRLPVVAGVGHSPG RMTGRVVRRCLLSATNVASPRKEKRPTGGQDRSGATSGRIPEKNDWQGTNERRTRPGY RIDPAPATATESGRSSAPSVRVAQRGHLPR" gene complement(5506..5745) /locus_tag="Psed_7014" /db_xref="GeneID:10361789" CDS complement(5506..5745) /locus_tag="Psed_7014" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004336636.1" /db_xref="GI:327309737" /db_xref="GeneID:10361789" /translation="MAQPRKLPDTHVLVTLAEKGLSNREIGELFGTTRQAVELRLQGL DVKRPPRVTCSACKGLGTVAPLYAGDSATRDLAPQ" gene complement(5745..5930) /locus_tag="Psed_7015" /db_xref="GeneID:10361790" CDS complement(5745..5930) /locus_tag="Psed_7015" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004336637.1" /db_xref="GI:327309738" /db_xref="GeneID:10361790" /translation="MSDEIKAGRYEIRYRAGKQTYASFTDDRVSAIRQLIAARRLSGR AACLDHETQKLITEDDE" gene complement(5927..6313) /locus_tag="Psed_7016" /db_xref="GeneID:10361791" CDS complement(5927..6313) /locus_tag="Psed_7016" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004336638.1" /db_xref="GI:327309739" /db_xref="GeneID:10361791" /translation="MALVSASALICPVCHEGAQDAPPSEWRVPGTVPAWSHSRDASAL CDVIGPDGYVPADPVEVCPRTLTSDETRTLRGIDRNYGPFRDDGWTVGDVIELISALD RETGGELAWCDGAAILAYMRSIGCAR" gene complement(6319..6552) /locus_tag="Psed_7017" /db_xref="GeneID:10361792" CDS complement(6319..6552) /locus_tag="Psed_7017" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004336639.1" /db_xref="GI:327309740" /db_xref="GeneID:10361792" /translation="MIHDMSQSATPADTQRDRLALGVRVPAPCASCSGALTARLPHRV TRRAAGTVELTCERCGAADTVPAALAGRLMAGE" gene 6895..7506 /locus_tag="Psed_7018" /db_xref="GeneID:10361793" CDS 6895..7506 /locus_tag="Psed_7018" /inference="similar to AA sequence:KEGG:CMS_pCSL0098" /note="KEGG: cms:CMS_pCSL0098 putative partitioning protein ParA" /codon_start=1 /transl_table=11 /product="putative partitioning protein ParA" /protein_id="YP_004336640.1" /db_xref="GI:327309741" /db_xref="GeneID:10361793" /translation="MRITVANLKGGVGKTTTSVMLALGFAKHGRTLLIDADPKQSSAL EWAGLADDWPLNCSVVAISGKGLAQRAASLVDDYEHVIIDCGAKSSLETRQALMVTDQ LIVPASPRALDIVEIPTTFTLAAEIDAVSPVDAVVLLTQVRAATRSSVDVRAVLLERD IPTLRSQVRLREAFAGAYGTVPDSLMDYDDVLTELIGGEKDGR" misc_feature 6898..7290 /locus_tag="Psed_7018" /note="ParA and ParB of Caulobacter crescentus belong to a conserved family of bacterial proteins implicated in chromosome segregation. ParB binds to DNA sequences adjacent to the origin of replication and localizes to opposite cell poles shortly following...; Region: ParA; cd02042" /db_xref="CDD:73302" misc_feature 6901..7386 /locus_tag="Psed_7018" /note="CobQ/CobB/MinD/ParA nucleotide binding domain; Region: CbiA; pfam01656" /db_xref="CDD:145019" misc_feature 6919..6939 /locus_tag="Psed_7018" /note="P-loop; other site" /db_xref="CDD:73302" misc_feature order(6937..6939,7144..7146) /locus_tag="Psed_7018" /note="Magnesium ion binding site [ion binding]; other site" /db_xref="CDD:73302" gene 7496..7951 /locus_tag="Psed_7019" /db_xref="GeneID:10361794" CDS 7496..7951 /locus_tag="Psed_7019" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004336641.1" /db_xref="GI:327309742" /db_xref="GeneID:10361794" /translation="MVGKRPRSVNFGDALDNKAAGLADDTPGDVPADTTEHVSGDVRG DMSDNVRDDMRPDSAWSMRSQSSGDKLDDVFSAYASARESASLRVRKVSVLMTDAQRD ALEQWIGRASKAVPGRVTASDVGMHLFDELVNDPVLTRRVLERLIAGIK" gene 8035..8874 /locus_tag="Psed_7020" /db_xref="GeneID:10361795" CDS 8035..8874 /locus_tag="Psed_7020" /inference="similar to AA sequence:KEGG:SAV_2075" /note="KEGG: sma:SAV_2075 hypothetical protein" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004336642.1" /db_xref="GI:327309743" /db_xref="GeneID:10361795" /translation="MFNLTGRPESSGRRPRWIIAVIGAALGVVILAPVALSFSSLVAW AEAALGLNGPWPYIVPVALDAAALLSMALTFHAVTHAESATFPRALVWVFALGSAFAN HRHGETVGPDAAIFFPAMPIAAAVLLEVTLRRVRRTVLAELGVTERPLARFRLVRWAR FPRETFAAWSTAVEHGLSSPIDALAVAARIEPLDADEIAHAVESRNLAALPSDAARVR RAATVTGQDDPGPVVAWLGAHGHPVTLDAARSALRRRPAARPPVPVSTNGHPVIVRSP ETL" sig_peptide 8035..8172 /locus_tag="Psed_7020" /note="Signal predicted by SignalP 3.0 HMM (Signal peptide probability 0.993) with cleavage site probability 0.969 at residue 46" gene 8976..9425 /locus_tag="Psed_7021" /db_xref="GeneID:10361796" CDS 8976..9425 /locus_tag="Psed_7021" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004336643.1" /db_xref="GI:327309744" /db_xref="GeneID:10361796" /translation="MNTTVSHGPVPRPPAPAHPRVTAWSGAATFVVLAGIARGIAGWP VLAAVPLAAVAAVLVTVALYWHGDRGGREYERRTDRVGAVARTFYLIVGRIPTDDELA SLVSDRPVTTLGGHATTPRPVRPGPLPGGVRLLSIVGPSDGGNGGAA" gene 9425..11407 /locus_tag="Psed_7022" /db_xref="GeneID:10361797" CDS 9425..11407 /locus_tag="Psed_7022" /inference="protein motif:PFAM:PF01580" /note="PFAM: Cell divisionFtsK/SpoIIIE; KEGG: mil:ML5_1658 hypothetical protein" /codon_start=1 /transl_table=11 /product="cell division protein FtsK/SpoIIIE" /protein_id="YP_004336644.1" /db_xref="GI:327309745" /db_xref="GO:0000166" /db_xref="GO:0003677" /db_xref="GO:0005524" /db_xref="InterPro:IPR002543" /db_xref="GeneID:10361797" /translation="MFIPRAGTSFRTVIIAGVLVSAVVNYTIQWFLGFPIGPRKTNAS FFHAGTELRKDATRATRWSYLPCWKRGVIRTIGTAIVAMFVWLYAAHRTAFTWTAWLS IVVALVAIVVLASRTAHGWQHRRTYVEPLSRALAPTLGIETPRNPASWITVPRSAVAP VPLITVPDKVREAAPMQLAARVWARRPEHIIPARLRREPDTAIRVQLPDGFTGSSDAQ STVTRIVSSKLGIPELNASFGMIGKPIATFTKKARPPEIAKWNDHQALVRAASESAPL IGVAANRKAVAVDLDAESPHILISSSTGGGKSVMARAIICQGLHNGGIALVLDVKRVS HAWARGLPNVRYCANVADIHNALLWVRGEVERRYTLIDDGADIDGNVGHIDLGPRVFI LAEEQNATITRLNEYWRETRESSDPKQSPAVAALNEVLFMGRACQTHVITIGQLMTAR ASGGPEARENYATRILARYSVNAWRMLVPECWPPPRSSRHSGRVQVVLAGKATETQVI FTTPGEAREYATSGIVTEFPALGGAPVAPPPLAPPVVSDEAAADDRSPGETTTSRGSQ GQNGAAPGVTLSEAAEAAVPLSLKALRSARDRDSEFPKPIGKRGRAWVYSPESLARWE RNRSTPGKQWAKDVSTPPKDVIDLSVERVKRKVDHA" misc_feature 10172..>10543 /locus_tag="Psed_7022" /note="P-loop containing Nucleoside Triphosphate Hydrolases; Region: P-loop NTPase; cl09099" /db_xref="CDD:158411" misc_feature <10253..10891 /locus_tag="Psed_7022" /note="DNA segregation ATPase FtsK/SpoIIIE and related proteins [Cell division and chromosome partitioning]; Region: FtsK; COG1674" /db_xref="CDD:31860" gene 12024..12938 /locus_tag="Psed_7023" /db_xref="GeneID:10361798" CDS 12024..12938 /locus_tag="Psed_7023" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004336645.1" /db_xref="GI:327309746" /db_xref="GeneID:10361798" /translation="MSRSPRVQIVCRGCGIPYQTRQRSNQTRCTKCGTTRYIPADQQW EGPTSTVPENMRASLSRTPVELNCYHCGHVWQSRAKSGGTTRCPECLRNRRIPSDARA TSDPAWQRDAALAARLADDPTDPAVLPPLPETGRYGAAERAATASNRRPRGKRQGRAH ETYTGDDGHEYDRVPAARRKREPCGECGKLHRAGTSCIKPRLTAEQKTAMRAEARETA RRDAEQFSAQMNAIRQAAAMVARMRGVSVPNVPVADATFDDDGQDDYTPAPVVAPRST GRVEYLPDTSGGQGVPITYIDISGKSES" gene 12935..13573 /locus_tag="Psed_7024" /db_xref="GeneID:10361799" CDS 12935..13573 /locus_tag="Psed_7024" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004336646.1" /db_xref="GI:327309747" /db_xref="GeneID:10361799" /translation="MTASEKFVGSPEHVKQAIDFIQAEFLLRDSDVHVVNITTADFYG GFETLVRLNNQLVSGARTLPTGKESNNWEKYNVQFVIFNKKISTRPWGDGYTGEPYAG LGGDFTDRVQVKFKEVGTTPDSRADTQQEIADPLPGATNALDTAWGMFTGLADFLKML QDPGTWVRLAAAITGGAMLLVVAWKLVGARQLVGSADGGGLKSALKIAQAVR" gene 13607..13912 /locus_tag="Psed_7025" /db_xref="GeneID:10361800" CDS 13607..13912 /locus_tag="Psed_7025" /inference="ab initio prediction:Prodigal:1.4" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004336647.1" /db_xref="GI:327309748" /db_xref="GeneID:10361800" /translation="MTVVMAIAGATLLYGAIKNRNPIDVIKLTLQGKDIKGAKPLSGS GDPNNLTAPPGAIPGGSLPGTPQAGDDARTDPKSFDPKRSDVLPRLPGEGNYRAQPY" ORIGIN 1 cccccgctcc gcgggtgggc ggctcgcgcc tgcggcgctc gtgtgtgtcg cgctgcgcgc 61 tcggtgtgcc gtgtttgtgc tggtcagagg ccgtctggtg tggacgtatt ggcgttcaat 121 gtgcattgtc cattccggac gcggaattgt tttgtgggta ccccccgggg gtttgtcgta 181 ttcaattgtg gcagggagga tgccgggagg atagggagga tgggtgtgcg ttcctccctg 241 tgttcctccc tgtcgggtta gggctgtgac ctgcggaaat ggccgttcca agatcgcgga 301 gggaggatag ggaggaactt aggggagacc tggacccttt atataggggc gaaattcggg 361 ctcctataga gcacaggtca aaagttcctc cctatcctcc ctgaacgatc actgtagggg 421 tgtttatgca ggtcagaggg ggtgccggga cagggaggaa ggcagggagg aacagggagg 481 atagggagga tagggggccg ggttcctccc tgacgcgggc aggcggcggg tcgttgctgg 541 attggcggcg gattgttgtc ggggaataag aaacggcccg ccggagattc cgacgggccg 601 ttacgtctgt ttctttaact agggggagct gcggtctttt cctgtagcca tgaggcaatg 661 agcttgaggt ctccgtacgt tccacgcgca cgaatatctg aacttaccag gaggacggcc 721 tcatccattt tatggacagg acaccattta accaagtggc atgctggctg gataaggagc 781 cgagacgctt gctgcatact ctcacgcgcg gctgtcgcga acgccatcgc cgcgccgtgg 841 tcagggaaga accacggact atcaggggcg cagtaaagcc cctttgttat ggtgactgcc 901 cagccgtgga caatcacccg gtctgtcttt gcgcctgttt ccggcggaaa cttgcgggtt 961 gtggggacga tcgcatgata gcgcccgtca gtgcccgtcc cccatcgatc ggggaatgag 1021 gcgccactac cgccgacgag tcgcgcgatg caagttcttt cctggattcg cattctattt 1081 ctcgcacccc attaatctag ccacattgaa cggacgtcaa agaatcgaac ttccgctccc 1141 tcactgcgac ggatcgtttc ggagagcgct tccgcaactc tcgcactacg catctcgcga 1201 taagcactct cgatagcccg ttgatttgcc gcgctaggag cttgcgtgcc ctgataccaa 1261 cggagcacgg tttcccgcga tggattaagt ccagacctca tgacggcgtc ctcggcgtgt 1321 cgtccgccgg tgtagagcgc gtccagttga gtgcgccatg atttggcatc cctgcctagt 1381 gcgcgggcga cgatactggt gtccacgttc gggaaggtga cggtccgttc ggggcgtgcg 1441 ttatataccc cttcccattc aattttcgcg tggaccgtta ggtctcgctg ttcgtcggcc 1501 cacgcttgat attcgtagtc ggcccatgct cgttcgattt cggcttccag cgcggaggcg 1561 ctttccgggt catactcgct ggggaggccg ctgttgcctg atttcggcat tgggattcct 1621 tattcctctc cgtcgctctt aaccttgaac gagccgagtt ttagcccaag ggggtcgtga 1681 acgattttgc ttccctcggc gaacaccgct gcacgtgctc gcttggggat gacgatttcg 1741 tccactcgct gaactgcgac ggggccgctc ttgggcgcaa gcctcaggca ttcatcggcg 1801 cgccgccaga gcatggcact agcgtgtgcg tacacggcgt gagtccagtc cggccgccgt 1861 gcgcggcatt cccacttgcg cggctttccg ggttccccgt ctccgagcat cagggttagc 1921 gcccgagaga atcccctctt ttgtagcgtg tactgttcgc tgtcgcgccc gtactcgtta 1981 atgacgtcct gtcgaagctc cttgataaag tccgaccatc ctcctgcatt cagacggaca 2041 ctagtttccc cggtgtacga atccagaatg tcgaactcag gccagcgtcc ggcgttgacc 2101 agctcttgga gaaggcttgc gtggggagcg gtaatccaag cggtcttggc tgctttgtcg 2161 attcccggga tggggtgcgg gagtgactcg gattcagtcc aagggaaaag agggacctcg 2221 taatacccgg ggagtccagg gtattgatcc atcgccccgg tgtgctctag cccgccgtgc 2281 gatacgtcga cgctagcgat tgcggcgagg aacgcgccag accggtctag cgtgatggtc 2341 tggccacgga acgggcggcg ccaattgtgg tttttcacaa ttgcggcaaa ggcaatgccg 2401 ggcatgcgtt ctggcgacca gcgtggttca cgtccggacc tgagtggtgc gcacggtcct 2461 tccagcgcat gcaacactga cgggatgcgg ttttcggaca gcgccgcttt gctgctagtc 2521 atgacgcgag tgcgcgcggc ggggcgctcg tcgggcgtga gcgcgcgccg cacagacggc 2581 gcctgagtcg gcgccggggt gttccgagtg gacggtgcct gagtcggcac gggggtgttc 2641 tgaccgacgg ccgtgcgggt gtccagctcc cccacggccc gcagcgcagg gcgggagtcg 2701 tccccctcgt tgtcgcctct cgcagacccg gtgacggact cgtccggagc cgcgaccgct 2761 actaggtgcg tgcgctcgtt tggctttagc ccgcgcatcc cgtcaaagac aacacgcccg 2821 tcactcttgc gggcaaggac cttgttcttc atgtattccg gccgttgctt tagttcaata 2881 ctgaatgctc gctgggatcg cgcgtgttct cccgcctgac ggcaccacca cacgtaatgc 2941 gcgtacaagt caccactagt ggtgatttcg ccgggtcgga aaatgcagtg atcggacaga 3001 aacgcgccca tcaaatcctg ctcgacgcgg tagtcttccg cagccgcgcg ccatgtgtcg 3061 ggcaaggcca aagtcttatt tgacgcccat gcccgagcac cttctacggc ccacgtgagg 3121 acgccactaa ggtcggacgg atctccgtta aagaggccga ttagcttgtc cgtcaaatgg 3181 ggatcacgct cctccggggt gatgtactcc cggaatggga tgagggcgag ccttgaccaa 3241 atgccctcat ctgtcccgcg aacggacggc cgatagttcg tcagtagcca aatcttgaac 3301 gtcggacgga aactgaagaa atctccataa aggaaccgcg cagtgattgt gcgatcgcct 3361 gtcagttcct ttacgcggga ctcggcgagt cggtcgccgc gctccgtttc agacgccata 3421 accatccggg caccgcgcat gtcggccatg tccggagacg cagaagttcc gtcctctttg 3481 cgggcggtca ggaaaccggc gggtgccttg cggtagtagt caccgagcaa gtgagccacg 3541 gtgtccaata gaacgttctt cccgttccgt ccgccaccgt tggcagtgaa caacttagcc 3601 tcaccggtaa acccggttag cgtgtagccg acacagagtt ggagtgcgcg ccgttctacg 3661 gggtccggct ggattcgtgc caggaatttg tcgaacagtt ccgaccgtgc atccggatcg 3721 taggtggcgg gggaaatgcg agtaatcatg tcgccccggg aatgttccct caactcgccc 3781 gtacggagat cgatcgtgcc gttttggacg ttgaatgccc acggatcact gtcaaaggca 3841 tcggcatcgg tgaccgtgat ttccggttcc gtcctggcca attcaatcat tgacgccaga 3901 cgcaggcggt tgccgctggc atccgcaaat ttcagcaact cacgagcgga ggtctcggcg 3961 gccttcctct cgtcgtgaga cagttcttta ctcgcactaa tggcgcgata tgcttgcttc 4021 cgcatttcga ggggcacgcg cttggcttgc tcctggacac ggtaatcctg gtcccgggca 4081 aaacgctttc catcccatac gtaccaagta ccgcggaagt ggcggacgtc gtcgccgcac 4141 agttccacta gtcgccgtgc gttccccatg tcgttaaagg tgtgaggcgc cgcccggggg 4201 ggcttgcgca tgctattatc gctcatcaga ggaaaccttt tcggtatcga atgccgggcg 4261 ttggcggcgc ccggcattcg tcgtgtcaga gataggtaac acggaaaata aatgcgcaga 4321 tgatgagcac cccgaaatgg tgaccaccag tatgtaacgc cgcacgtcga acgtggttaa 4381 taaatatgcg ccgggccgat tcctcggaaa tgccgcttgc ggtgacgctg caaagctcct 4441 gtcgtgcagt cgaccagaag tagcggtcaa tgcgtacgcc agggctatcg gggagcggat 4501 tagcgagtgc gtccacgtca acgcgggagg tggccgcgtt gagcgacgcg gaccgacggc 4561 gccgaggaac ggcccgattc ggtcgcggtg gccggggccg gatcaatccg gtatcctggt 4621 cgtgttcggc gttcattggt gccctgccaa tcattctttt cggggatgcg tccggacgtt 4681 gcgcccgatc ggtcttgccc accggtcggg cgcttttctt tacgcggaga ggccacattc 4741 gtcgccgata gtaggcaacg ccggacgact ctcccggtca tcctcccggg ggagtggcct 4801 acgcccgcga cgacggggag cctgtgcata tgcgtcccgg agaacttcct cactgacgcg 4861 ctggtccagg acagtgagga gcgcggcgtt aataagcgtg gcgactgaat cagtgaactt 4921 gaggcccaac cgcgcggccg cctcttgcat aacgtcatcc gggaaatccc cgttgattcg 4981 tcgcaaccct ggcgcccgca ttgcctttcc cataactcac tctccctgtc ggcgatatcc 5041 cgatgatatg cccattcact gggtgcgtgc catgcgcgtt ttgcctccat tcgagtgaat 5101 tcaaaatggg gcgccgattg acactaatgc atctattccg cgctctgtcg gggatattct 5161 tatttcctcc ccggactctt tatctgcgat taccaatacg ggtcggccgg cgtcgctatc 5221 cagggcgatt ccgacgcggt cattctctcc gatgagcgtc ctagttattg tcattggtct 5281 tcctgttccg tcgagattga cgggcagaac gaatctgtgc attagtggcg atgccgtcgg 5341 ggccgcttgc gtcgacaacg gccgtgagcc caaacgcgcg catgatcggg gcaacccatg 5401 tcatcgcgga tcgatggccg agacggccgc agggaccgca cgacgcatta ccgcgtcggc 5461 ggtccctggc gcacccgcac gggcactgac agcggctgtt agcggtcatt gtggtgcgag 5521 atcccttgtc gcgctgtcgc ccgcgtacag cggagcgaca gttccgagtc ccttgcacgc 5581 agagcacgtg acgcggggcg gtcgcttgac gtcgagtccc tgcaaccgca attccacagc 5641 ctgccgcgtg gtcccgaaca actctccgat ctctcggtta ctcaacccct tttcggcgag 5701 agtgaccagc acgtgagtat ccgggagctt acggggctgg gccattattc gtcatcctcc 5761 gtgatgagct tctgcgtctc gtgatcgaga cacgccgccc ggccggaaag tctgcgagcg 5821 gcaataagtt gccgaatcgc ggacaccctg tcatcggtga atgatgcgta agtctgtttg 5881 cccgcgcggt agcggatttc ataccggccc gccttgatct cgtcgctcat cgggcacacc 5941 cgatcgaacg catgtaggcg agaatggcag cgccatcaca ccaagcgagc tcaccgccgg 6001 tctcccggtc tagagcggaa ataagctcga taacgtcgcc gaccgtccac ccgtcatcgc 6061 ggaacggacc gtaattgcga tcgatgccgc gcaatgtccg ggtctcgtcg gaagtgagcg 6121 tgcgcgggca gacctcgacc ggatcggcgg ggacatagcc gtcgggtcca atgacgtcgc 6181 acagcgcgga cgcatcgcgg gagtgcgacc aggcgggcac cgtgccgggg acgcgccact 6241 cagacggcgg ggcgtcctgc gcgccctcgt gacagaccgg gcagatgagt gccgacgcgc 6301 tgaccagagc catcccgatc actcccccgc catcaggcgg cccgcgaggg cggccgggac 6361 ggtgtctgcg gcgccacagc gctcgcacgt cagctccacc gtgccagcgg cccggcgcgt 6421 cacccggtgc gggagacgcg cggtcagggc gccggaacag gacgcacacg gggcagggac 6481 cctgacgccg agcgccagtc ggtcacgctg ggtatcggcc ggcgtcgcgc tctgtgacat 6541 gtcgtggatc atggttactc gtctccctgg cagggtgatg cagtgccgta cgtgtgctac 6601 tgtaacacat gtagcgtcat gcagcgcagc gaccggcacc acgtccggac gcaacatagc 6661 aggagtcacg gcagggagtg tgaacgtgag taacagagag agtgccacta agccggtccg 6721 aatgtttccg gcgacgcatt cggcgtttca gcatatgacg ctttccgtca gtgttggtgt 6781 cgggcgtcgc gtcactatcg cggaagtggt tgccgccgct gctcatgtgg cgtcacagca 6841 catgacggaa gtggaagagt acattaagcg cgtcgattcg gaagttgacg ccggatgagg 6901 attaccgtcg cgaacctaaa gggcggcgta ggtaagacga ctacgtcggt aatgctcgcg 6961 ctaggtttcg caaaacatgg ccggacacta ctcatcgatg ccgacccgaa acagtcgtcc 7021 gcattggagt gggccgggct cgccgacgac tggccgctga actgttcggt cgtggcaatc 7081 tccggcaagg gtcttgcgca acgggccgca tcgctggtgg acgactacga gcacgtcatc 7141 atcgattgcg gcgctaaatc ctcccttgag acgcgacagg cgctcatggt gaccgatcaa 7201 ctcattgttc ccgcgtcacc gcgcgctctc gatatcgtgg aaattccgac gacgttcacg 7261 cttgcggcgg agattgatgc cgttagccct gtcgacgcgg tggtgctgct aacgcaggtg 7321 cgagccgcta cccgttcgtc cgtggacgtg cgcgcagtgt tgctagagcg agacattcct 7381 acgttgcgtt cgcaggttcg attgcgtgag gctttcgcag gtgcgtacgg cacggtcccg 7441 gacagtctga tggattacga cgatgttctg acagaactta tcggaggcga gaaggatggt 7501 cggtaagcgt ccgcgctccg tgaacttcgg agatgcgctc gacaataagg cggcgggtct 7561 ggccgatgac acgcccggcg atgtgcccgc cgatacgaca gagcatgtgt ccggcgatgt 7621 gcgcggcgac atgtcagaca acgtgcgcga cgacatgcgc cctgacagcg catggtctat 7681 gcgctcgcaa agttccggcg ataagctaga cgatgttttc tccgcgtatg cgagcgcacg 7741 tgagagcgca agtctccggg tacgtaaggt ctcggttctg atgaccgatg cgcagcggga 7801 tgcgctcgaa caatggatcg gacgtgcgag caaagcggtg ccgggccgtg taaccgcgtc 7861 ggacgtcggg atgcacctgt ttgacgagtt agtgaatgac cccgtgctca cgcgccgggt 7921 gctggaacga ctcatcgccg ggataaagta gtaaaccgtt cggtcgccac tcctgccagg 7981 gaaacgtaag cgaccgaacg gccccacaga aaccctcact aggagagtcc tgcaatgttc 8041 aatcttaccg gccgcccgga atcctcggga cggcggcccc gctggattat tgcggtaatc 8101 ggcgcggcgt tgggcgtggt cattcttgcg ccggtcgctc tgtcgtttag ttccctggtc 8161 gcgtgggcag aggccgcgct agggcttaat ggaccatggc cgtacatcgt gcccgttgct 8221 ctcgacgctg cggccctact ctctatggcg ctgacattcc acgcggtgac acacgcggag 8281 agcgcgacgt tcccccgcgc actggtgtgg gtgttcgctc tcggatcagc gttcgctaat 8341 catcgccacg gtgagaccgt ggggccggac gcggccattt tctttccggc aatgccgatc 8401 gctgcggcag tgttgctaga ggtcacgctc cgccgcgtgc ggcgaacagt cctcgcggag 8461 ttgggcgtga cggagcgccc gctcgctcga ttccggctag tccgatgggc gcggttcccc 8521 cgggagactt tcgcggcatg gtcgacggcc gtcgagcacg gtctctcgtc gccgatcgat 8581 gcgcttgccg ttgcggccag gattgagccg ctagacgccg atgagattgc ccacgcggtt 8641 gagtcccgga atctcgccgc tctgccatcc gatgcggcgc gggttcgccg cgcggccacc 8701 gtgaccggcc aggacgatcc cggcccggtc gtggcatggc tgggcgccca cggtcacccg 8761 gtcaccctgg acgcggcccg gtcggctctg cgccgtcgcc cggccgcccg gccgcctgtc 8821 ccggtcagca ccaacggcca tccggtcatc gtccggtcac ccgagacgct gtgaccgccg 8881 tgcgaccgcg cccgacaacg aacggggccg gtccccgtag gaaccggccc cgccgacccg 8941 tagtaaccca acctctcaca gtaaggaaac cgcaggtgaa cacgactgta tctcacggcc 9001 ccgtcccgcg accgcccgcg cccgcgcacc cgcgcgtgac cgcctggtcc ggcgcggcaa 9061 cgttcgtcgt cctcgccggg atcgctcggg gcatcgccgg gtggcccgta ctggccgccg 9121 tcccgctggc cgccgtggcc gctgtgctgg tcactgtcgc tctgtactgg cacggcgacc 9181 gtggcggccg ggagtatgag cgccgcaccg atcgggtcgg cgcggtggcc cgcacgttct 9241 atctcatcgt tgggcgcatc ccgactgacg acgagttggc atcgctcgtg agcgaccggc 9301 cggtgaccac cctcggcggg cacgcgacca cgccgcgacc ggtccgcccc ggcccgcttc 9361 ccggcggggt gcggctcctg tccattgtcg gcccgtccga cggtgggaac ggcggggccg 9421 cctgatgttc attcctcgcg cgggcactag tttccgcaca gtaataatcg ctggggttct 9481 agtctctgct gtcgtcaact acaccattca atggttccta ggattcccga tcggcccccg 9541 gaagactaat gcgtcgttct tccacgcggg caccgaatta aggaaggatg cgacgcgggc 9601 aacgcgatgg tcctatctcc catgttggaa gcgcggagtt atccgcacta ttgggaccgc 9661 gattgtcgcg atgttcgtat ggctctacgc ggcgcaccgg acggcgttca cctggactgc 9721 gtggttgtcg atcgtcgtcg ccctggtcgc gatcgtcgtc ctggcgtccc gcaccgcgca 9781 cggctggcag caccgacgca catacgtcga gccgctgtct cgcgcgctcg caccgacact 9841 cgggatcgag actccccgca accctgcatc gtggatcacc gtcccgcgca gcgccgtggc 9901 gcctgtcccg ctcatcaccg tgccagacaa ggtccgagag gcagcgccca tgcaactcgc 9961 cgcccgcgtg tgggccaggc gcccggaaca catcatcccg gcccggctgc ggcgcgaacc 10021 tgacaccgcg atccgcgtgc agctccccga cgggtttacc ggaagctctg acgcacagtc 10081 cacggttacg cggattgtgt ctagcaagtt gggcattccg gagctgaatg catcgttcgg 10141 catgattggt aagccgatcg ctacgttcac caaaaaggct cgaccgccgg aaattgccaa 10201 gtggaacgat caccaggcgc tcgtgcgtgc cgcatccgag tctgcgccgc tcatcggcgt 10261 tgccgctaat cgcaaggctg tcgcggtgga ccttgacgcc gagtccccgc acattcttat 10321 ttcgtccagt accggcggcg gtaagtccgt catggcccgc gccatcattt gccaggggtt 10381 gcacaacggc gggatagctc tagtcctgga cgtcaagcgg gtatcccacg catgggcgcg 10441 cggactcccg aacgtccgct attgcgcaaa cgtagcggac attcataacg cactgttgtg 10501 ggtgcgcggg gaggtggagc ggcgctacac gctcattgac gacggcgcag atattgacgg 10561 caacgtcgga catatcgatt tgggcccccg tgtctttatt ctcgcggagg agcagaacgc 10621 cacaatcacc aggcttaacg aatactggcg tgagacccgg gagtcctcgg acccgaaaca 10681 gtccccggcg gttgcggcgc ttaacgaggt gctgtttatg ggccgcgcgt gtcagacgca 10741 cgtaatcact atcggtcagc taatgacggc tcgcgctagc ggggggccgg aggcccggga 10801 gaattacgct acccgcatcc tcgctcgcta cagcgtgaac gcctggcgca tgttggttcc 10861 cgaatgctgg ccgccgccgc gcagttcgcg tcattccggg cgcgtgcaag tcgtgctagc 10921 gggcaaggcc accgaaacgc aggttatttt caccaccccg ggggaagccc gcgaatacgc 10981 tactagcggc atcgtcacag agtttcccgc gctcggtggc gcgccggtcg cgcccccgcc 11041 cctggccccg ccggtcgtca gcgatgaggc agccgccgac gatcgttcac caggagaaac 11101 gactacctca cggggcagtc aggggcagaa cggggcggcc cccggagtca cgctctcgga 11161 agccgcagag gcggccgttc cgctctcatt aaaggcgctg cgctctgccc gggataggga 11221 ttccgaattc cctaagccta tcggcaagcg aggccgcgcg tgggtttaca gtcccgaatc 11281 actcgcaagg tgggagcgga atcgctctac gcccggaaag cagtgggcta aggacgtgag 11341 caccccccca aaagatgtga ttgatttatc cgtagagcgc gttaagcgaa aggtagacca 11401 tgcgtaacga caactcactc ccgttgcccc tgctcactca ttctagggaa cacgccatgc 11461 ccctcccgac cattaatagt ttcattccag ttactcgccc acttccggcg gttagtcatc 11521 gtcggcgcgg accattccgg ctattgacac gggcggtcgc cggccttgtt atttgcggaa 11581 tcattctcgg actgttcgtc gcgtgtggag tatatattct tacccggggc gaaacgttga 11641 cgattccgga acacgggcct gtccccgcgc ccattgcgcc gccgtttgac gtgtcattgc 11701 cggaaatcca tgtgccggta atcgacggac accacgtgcc agcgcctacc cgcgccgcgc 11761 ctacggtttc agtgcccgtc cctccgtctg cggctaagac tccgccgccc gttgacaacg 11821 gcatcactcc gatcgagcca atggacccca tgcatagata gatgcgcacg caccattccg 11881 ccccggtcgc cgaacgcctg gcgaccgggg cggagtcatg tgcacactgc gatccgtggg 11941 ggtcacgaca aatgacgcgc aatccttgcg aaatacgcgg acctggcctg acaatgtgcc 12001 tacggtttag ggaagggggc cgcatgtcac ggagtccccg cgtgcagatt gtgtgtcgag 12061 ggtgtggcat tccctatcag acacgacagc ggagtaatca aacccgctgc acaaagtgcg 12121 gaactactcg atacatcccc gcagatcaac aatgggaagg accgactagt accgtgccgg 12181 aaaacatgcg cgcgtcattg tcgcggactc ctgtagagct caactgctat cactgcggcc 12241 atgtgtggca atcccgcgct aaatccgggg gaacaactcg ctgccccgaa tgtctccgca 12301 accgtcgcat cccgtccgat gctcgtgcga ccagtgaccc cgcgtggcag cgtgacgcgg 12361 ccctggcggc ccgcctggcc gacgacccga ccgaccccgc cgtcctgccc ccgctgcccg 12421 agacgggacg ctacggagcc gccgagaggg ccgccacagc atcgaaccgc cgaccccggg 12481 ggaagcgcca gggtcgggcg cacgagacct acacgggcga cgacgggcac gagtacgacc 12541 gcgtcccggc cgcccgccgg aagcgggagc cgtgcggcga gtgcggcaag ctccaccgcg 12601 cgggaacgtc ctgcattaag ccgcgactta ccgccgaaca gaaaacagcc atgcgcgccg 12661 aggcgcggga gacggccagg cgcgatgcgg aacagttctc cgcgcagatg aacgctattc 12721 gccaggcagc cgcgatggtc gcgcggatgc gcggtgtgag cgtcccgaat gttcctgtcg 12781 ccgatgccac gttcgatgat gacgggcagg atgactacac acccgccccg gtcgttgcgc 12841 cgcgctctac cggacgtgtc gaatacctcc ctgacacgtc cggcggccaa ggcgtcccta 12901 ttacctacat cgatatttcc ggaaagagtg agtcatgaca gcatccgaga agttcgttgg 12961 ctcacccgaa cacgtcaaac aagccatcga ttttatccaa gcggaattct tgctccgcga 13021 cagtgacgtc cacgttgtga acattacgac cgcagacttt tacggcggat tcgagacgct 13081 ggttcgtctc aataatcaac tcgtctcggg ggctaggaca ttgcctaccg ggaaagagag 13141 taataactgg gaaaagtaca atgtccagtt cgtaatcttc aataagaaga tcagcacgcg 13201 cccgtggggc gatgggtaca ccggtgaacc gtacgccgga ctcggcggcg atttcaccga 13261 cagagtccaa gttaaattca aagaggttgg taccaccccg gatagccgcg ccgataccca 13321 gcaagaaatt gccgacccgc tgcctggcgc cactaatgcc ctcgataccg catggggcat 13381 gtttacgggg ttagccgatt tcctcaaaat gttgcaggac cctgggacgt gggtgcgctt 13441 ggccgcggct attacgggag gcgcaatgtt gctcgtcgtc gcgtggaaac tcgttggtgc 13501 acgccaattg gtcggaagcg ctgacggggg agggctaaag agcgccctta agatcgcgca 13561 ggctgtcagg taatagagag gaacaattac ggtggaacta gaagtaatga cggtggtcat 13621 ggcgattgcc ggggcaactc tgctctacgg tgccatcaag aatcgcaacc ctattgacgt 13681 cattaaattg acgctacagg gcaaggatat taagggcgcg aaaccgctaa gcggtagcgg 13741 cgaccctaat aacctcaccg ctccgcccgg cgcaatcccc ggcgggagtc tccccggcac 13801 tccgcaagcc ggagacgacg cacggaccga ccctaagtca ttcgacccga aacgctccga 13861 cgttctccct cggcttcccg gagagggcaa ctatcgggcg cagccctact gatgggggag 13921 tctagataat gcttagcagt agcttttgga tgacaattgc tcattcttgg taacttcata 13981 aaggctcgca ggagcaatcc gtatcggcgc aactcctaaa acattcggga cggtgagcca 14041 atgtcggcag cagtgagggc caaggatatt cgcgcctact ttgcacgtct tccggaagcc 14101 gactttaatg aggcagctaa ccggcttgga attgttcctg aacgcggagc ggtgacaagc 14161 atcattagag ctgccctact cgtcgtgata catagcgacg atgagcacgc tattaacatg 14221 gccgcggaat gtgcaatgga tcgtggtaga agggggcacc cgaaatgatt gtctgcgaga 14281 attgtggcgg atggttcgat ccaatcgggt gccgctggtt gtgtccctgc tgtcgccata 14341 aagccaactg ttgcgaggga gcgccgcaat gatgaatcac gccgtccgaa agaatcagac 14401 gccggatcac atttccgtag tggaacccgg aaagtgctgg cactgcgaaa agcccacacg 14461 atatgtggaa gttaatttcg agacgtatct ctgtccgggg gagtgcacca atagtaaatt 14521 ggtggaatat atccgggact ccgcacgcgg aagcggagag taaagtctca caactaaata 14581 cataactcaa tcccacaact gaataaccca acctgggagc ggcccgccga tatttcacgg 14641 cgggccgttt tcttattgcc cgacagtaat ccgccgccat ccagcaacga cccgccgcct 14701 gcccgcgtca gggaggaacc cggcccccta tcctccctat cctccctgtt cctccctgcc 14761 ttcctccctg tcccggcacc ccctctgacc tgcataaaca cccctacagt gatcgttcag 14821 ggaggatagg gaggataggg ggccgggttc ctccctgacg cgggcaggcg gcgggtcgtt 14881 gctggatggc ggcggattac tgtcgggcaa taagaaaacg gcccgccgtg aaatatcggc 14941 gggccgctcc caggttgggt tattcagttg tgggattgag ttatgtattt agttgtgaga 15001 ctttactctc cgcttccgcg tgcggagtcc cggatatatt ccaccaattt actattggtg 15061 cac //