LOCUS NC_015218 12568 bp DNA circular BCT 05-JUL-2011 DEFINITION Lactobacillus acidophilus 30SC plasmid pRKC30SC2, complete sequence. ACCESSION NC_015218 VERSION NC_015218.1 GI:325955704 DBLINK Project: 63605 KEYWORDS . SOURCE Lactobacillus acidophilus 30SC ORGANISM Lactobacillus acidophilus 30SC Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae; Lactobacillus. REFERENCE 1 (bases 1 to 12568) AUTHORS Oh,S., Roh,H., Ko,H.J., Kim,S., Kim,K.H., Lee,S.E., Chang,I.S., Kim,S. and Choi,I.G. TITLE Complete genome sequencing of Lactobacillus acidophilus 30SC, isolated from swine intestine JOURNAL J. Bacteriol. 193 (11), 2882-2883 (2011) PUBMED 21478365 REFERENCE 2 (bases 1 to 12568) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (10-MAR-2011) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 12568) AUTHORS Roh,H., Ko,H.-J., Kim,S.-H., Choi,I.-G. and Oh,S. TITLE Direct Submission JOURNAL Submitted (28-FEB-2011) Computational and Synthetic Biology Laboratory, Korea University, Anam Campus, Anam-dong 5-ga, Seongbuk-gu, Seoul 136-713, The Republic of Korea COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to CP002561. Genome was manually curated based on annotation generated by the NCBI Prokaryotic Genomes Automatic Annotation Pipeline Group. Information about the Pipeline can be found here: http://www.ncbi.nlm.nih.gov/genomes/static/Pipeline.html. Bacteria available from Computational and Synthetic Biology Laboratory at Korea University in South Korea. COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..12568 /organism="Lactobacillus acidophilus 30SC" /mol_type="genomic DNA" /strain="30SC" /db_xref="taxon:891391" /plasmid="pRKC30SC2" gene 502..717 /locus_tag="LAC30SC_10880" /db_xref="GeneID:10271857" CDS 502..717 /locus_tag="LAC30SC_10880" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004293179.1" /db_xref="GI:325955705" /db_xref="GeneID:10271857" /translation="MKYELTEQSVQFTFGNKRYKSGLKNKTYANVDKDASADALTVVG QAIAGLQDDSLDDTILIQRRRVVESAE" gene 742..996 /locus_tag="LAC30SC_10885" /db_xref="GeneID:10271842" CDS 742..996 /locus_tag="LAC30SC_10885" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004293180.1" /db_xref="GI:325955706" /db_xref="GeneID:10271842" /translation="MTTTTKSLRLNFKNAKDKSTSITLANALENLPADKVKDAMQKIA GSNVFKKDGTVLYVTPVSANYIERTVTPVFNDEAEAKDAE" misc_feature 757..966 /locus_tag="LAC30SC_10885" /note="Protein of unknown function (DUF2922); Region: DUF2922; pfam11148" /db_xref="CDD:151591" gene 1078..1272 /locus_tag="LAC30SC_10890" /db_xref="GeneID:10271843" CDS 1078..1272 /locus_tag="LAC30SC_10890" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004293181.1" /db_xref="GI:325955707" /db_xref="GeneID:10271843" /translation="MKEYLVLTGYAQAVYRKCELCKKVKDVNYRLDVKHAESPVMLTG SLDLCKVCAANFGEIIKKGA" gene 1273..1416 /locus_tag="LAC30SC_10895" /db_xref="GeneID:10271844" CDS 1273..1416 /locus_tag="LAC30SC_10895" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004293182.1" /db_xref="GI:325955708" /db_xref="GeneID:10271844" /translation="MTQEILKYVLEQASGVVIALMLIMRIETKLDSLTSAIMRLSEPK SSK" misc_feature 1291..1398 /locus_tag="LAC30SC_10895" /note="YvrJ protein family; Region: YvrJ; pfam12841" /db_xref="CDD:193315" gene 1440..2294 /locus_tag="LAC30SC_10900" /db_xref="GeneID:10271845" CDS 1440..2294 /locus_tag="LAC30SC_10900" /note="COG4973 Site-specific recombinase XerC" /codon_start=1 /transl_table=11 /product="integrase/recombinase" /protein_id="YP_004293183.1" /db_xref="GI:325955709" /db_xref="GeneID:10271845" /translation="MQSKNYEKLIDEFIEDLRLQGRAEGTLLEYEMRLKEFAEFFQLK ALALDQVKKSDLTEFMEQKLSNCLKVSTIRGKLSTFRVFCLWAVNKNYLSEVIISPQD YPKNTNVKRIRRLSDENLTIFKHYIDTLQPNARAAFYLLIGSGCRVAEAANLKAEDVT LRGKSVYIDIRNAKWGSDRNIPIVDADAAKIVWKYRTEVEVDNRPLFRLSKRTLQNYA TNFAKSTGINFYCHLLRHTFAGLLTEKGVPLTTVQYLLGHKSLGMTAHYAQSARVDIT DITPEINL" misc_feature 1458..2240 /locus_tag="LAC30SC_10900" /note="Site-specific recombinase XerD [DNA replication, recombination, and repair]; Region: XerD; COG4974" /db_xref="CDD:34580" misc_feature 1467..2255 /locus_tag="LAC30SC_10900" /note="DNA breaking-rejoining enzymes, C-terminal catalytic domain. The DNA breaking-rejoining enzyme superfamily includes type IB topoisomerases and tyrosine recombinases that share the same fold in their catalytic domain containing six conserved active site...; Region: DNA_BRE_C; cl00213" /db_xref="CDD:193712" misc_feature order(1878..1883,1950..1952,2127..2135,2238..2240) /locus_tag="LAC30SC_10900" /note="DNA binding site [nucleotide binding]" /db_xref="CDD:29495" misc_feature order(1878..1880,1950..1952,2133..2135,2142..2144, 2211..2213,2238..2240) /locus_tag="LAC30SC_10900" /note="Int/Topo IB signature motif; other site" /db_xref="CDD:29495" misc_feature order(1878..1880,2133..2135,2142..2144,2211..2213, 2238..2240) /locus_tag="LAC30SC_10900" /note="active site" /db_xref="CDD:29495" gene 2325..2654 /locus_tag="LAC30SC_10905" /db_xref="GeneID:10271846" CDS 2325..2654 /locus_tag="LAC30SC_10905" /note="COG0629 Single-stranded DNA-binding protein" /codon_start=1 /transl_table=11 /product="putative single-stranded DNA-binding protein" /protein_id="YP_004293184.1" /db_xref="GI:325955710" /db_xref="GeneID:10271846" /translation="MNDIKLLGRLTGNPELKQSANGRVYAWFTVAVPRQNNRDEADFI RCVAFDKLAQAIAQHCKKGRQVLAHGRLQVSTSMDNTTQQTRYHHTVVVSNTHFLHDP NRATVAA" misc_feature 2340..2621 /locus_tag="LAC30SC_10905" /note="SSB_OBF: A subfamily of OB folds similar to the OB fold of ssDNA-binding protein (SSB). SSBs bind with high affinity to ssDNA. They bind to and protect ssDNA intermediates during DNA metabolic pathways. All bacterial and eukaryotic SSBs studied to date...; Region: SSB_OBF; cd04496" /db_xref="CDD:72968" misc_feature order(2340..2345,2418..2426,2448..2450,2454..2456, 2517..2519,2544..2546,2580..2582,2589..2591) /locus_tag="LAC30SC_10905" /note="dimer interface [polypeptide binding]; other site" /db_xref="CDD:72968" misc_feature order(2349..2357,2373..2375,2403..2405,2424..2426, 2442..2447,2451..2453,2457..2459,2463..2465,2469..2471, 2508..2513,2535..2537,2541..2543,2547..2552,2592..2594, 2598..2600) /locus_tag="LAC30SC_10905" /note="ssDNA binding site [nucleotide binding]; other site" /db_xref="CDD:72968" misc_feature order(2517..2519,2523..2525,2529..2531,2613..2615) /locus_tag="LAC30SC_10905" /note="tetramer (dimer of dimers) interface [polypeptide binding]; other site" /db_xref="CDD:72968" gene 2873..3883 /locus_tag="LAC30SC_10910" /db_xref="GeneID:10271847" CDS 2873..3883 /locus_tag="LAC30SC_10910" /note="COG2946 Putative phage replication protein RstA" /codon_start=1 /transl_table=11 /product="replication initiation factor family protein" /protein_id="YP_004293185.1" /db_xref="GI:325955711" /db_xref="GeneID:10271847" /translation="MENTALTVSIDWLEFTILNSQLHEVMKTMELKWDDFSKLSKGKF GYNNQIKWSDGSIFIMFTAKDEDVNESTLINPKSGVHVMITGKGCRQYSVNHDLISLI KRLYSLPRVNFSRIDLAIDDFESKIINYDRIHEAAINGHFTSRWSKWDEINSRQTSDN HFLGRTMYFGSQVSEIFCRIYDKTLERKAKSDETEIPKNWTRLEMVYRKERATKLVDF LVNKNMPIGYVLRGTLKHYLRFILPSTDRNKARWRTTAWWEELLSSVDRLQLTVKKEA RTIDDMTAWIDQQISPTLSAILKAQEGDLGWLRSILAKGSKRLTQKHKDAINQYLKGM TV" misc_feature 3194..3787 /locus_tag="LAC30SC_10910" /note="Replication initiation factor; Region: Rep_trans; pfam02486" /db_xref="CDD:190324" gene 3880..4641 /locus_tag="LAC30SC_10915" /db_xref="GeneID:10271848" CDS 3880..4641 /locus_tag="LAC30SC_10915" /note="COG0739 Membrane proteins related to metalloendopeptidases" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004293186.1" /db_xref="GI:325955712" /db_xref="GeneID:10271848" /translation="MTSTSQSYPRLAFCSAITPADPAHYLNLRKAGIDTASICLHVSG FEYYKFATIHTQLARKADLVTHAFMITDLLDPFADVTTFFKRFNQLGYDSNAKITVWV NGDKYVADRESKIIEIIDLIAKCHNRENIDLAFFKRDIDDKLYDLSKMPQMINLTIIN CEGTGAGIDIAGTWIYTMDFCNEVQVLGYDYYGYYTDDAGYQLSLVDTDYVVQEGDTW YSISRRHGIPLNDLLALNRAIETERVFAGQVVRIA" misc_feature 4501..4635 /locus_tag="LAC30SC_10915" /note="Lysin domain, found in a variety of enzymes involved in bacterial cell wall degradation. This domain may have a general peptidoglycan binding function; Region: LysM; cd00118" /db_xref="CDD:29017" misc_feature order(4507..4509,4516..4518,4525..4527,4540..4542, 4549..4551) /locus_tag="LAC30SC_10915" /note="putative peptidoglycan binding site; other site" /db_xref="CDD:29017" gene 4987..5463 /locus_tag="LAC30SC_10920" /db_xref="GeneID:10271849" CDS 4987..5463 /locus_tag="LAC30SC_10920" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004293187.1" /db_xref="GI:325955713" /db_xref="GeneID:10271849" /translation="MKVYPWLDSIAAPVVGTKYALGEGCELLNKLDDTGWVIDGTESS YMLEEAYVYEHIAEGMLLPEPENPVDPKAVAVYLRFVATKKSMRPHKMAVRIGYLPEE SRYKKCIKKATMVKIHCRDMIFGTDPARYFDAEVVDVPLKLTSKEYECMAMDLYLE" gene complement(5975..6973) /locus_tag="LAC30SC_10925" /db_xref="GeneID:10271850" CDS complement(5975..6973) /locus_tag="LAC30SC_10925" /codon_start=1 /transl_table=11 /product="restriction endonuclease (HaeIII)" /protein_id="YP_004293188.1" /db_xref="GI:325955714" /db_xref="GeneID:10271850" /translation="MANTESGKAFEYACLLAIYDFYKEKEVPVEIIESPQLETAKSYF DALSDHGKNDLLAGEAAIKLINLLEPKLDHYADNSVLKLTLQTDSNGQKGDVRDILCI RNDSKRKSWELGVSCKHNHFALKHSRISPTIDFGKEWLGKQCSSEYMDEVGRIFANLK PYVKEKWDKVSDKVDKVDDVYKPLLKAFMDELTRLDEEYPGEIAPALVNYLIGEKDFY KVISVEKKHITLITPVNLHDSLNERYKDHRSMFSIEKPKLPSVIYHIGFKKVLDKKTN AINQSDNTIIVTCDEGWSFSLRIHNASSKVENSMKFDIESVSTPESIRSFIAPWNV" misc_feature complement(6014..6955) /locus_tag="LAC30SC_10925" /note="HaeIII restriction endonuclease; Region: RE_HaeIII; pfam09556" /db_xref="CDD:118092" gene 7075..8295 /locus_tag="LAC30SC_10930" /db_xref="GeneID:10271851" CDS 7075..8295 /locus_tag="LAC30SC_10930" /note="COG0270 Site-specific DNA methylase" /codon_start=1 /transl_table=11 /product="modification methylase BsuRI" /protein_id="YP_004293189.1" /db_xref="GI:325955715" /db_xref="GeneID:10271851" /translation="MKKFESSLTFSAEKVKKILFAKIEEEKEKIENNKTEENVKLDKE LYDKASALYDDSKINVLSLFSGAGGLDLGVEIAAASAKHGVDKTYTAFQNRRELSKLL DSTNVIYSNDLFASANATYKDNFSGNYVKDTRDVRKVISFPKADLVLGGFPCPGFSVA GPRLLDDPRNFLYIHYIRALMDSTPKIFIAENVKGLMTMAGGKVLSQMKEDFSAAGYK VTANLVNAKDYGVPQSRERVFLVGVRNDIADKFQYTVPEPTNGPDTDKPYITLKEAIS DLPEHPADYFEGTYSSIYMSRNRKKSWDDVSFTIQASARQAPQYPGGKPMVKIGRDKW EFQGDLNRRLSVKECARIQTFPDWFEFSDGGKDNVSLANRLNEKYKQIGNAVPVLLAE KIARPVMQFLENYK" misc_feature 7246..8262 /locus_tag="LAC30SC_10930" /note="Cytosine-C5 specific DNA methylases; Methyl transfer reactions play an important role in many aspects of biology. Cytosine-specific DNA methylases are found both in prokaryotes and eukaryotes. DNA methylation, or the covalent addition of a methyl group...; Region: Cyt_C5_DNA_methylase; cd00315" /db_xref="CDD:73191" misc_feature 7246..8262 /locus_tag="LAC30SC_10930" /note="C-5 cytosine-specific DNA methylase; Region: DNA_methylase; pfam00145" /db_xref="CDD:143915" misc_feature order(7264..7281,7405..7416,7465..7476,7525..7527, 7531..7533) /locus_tag="LAC30SC_10930" /note="cofactor binding site; other site" /db_xref="CDD:73191" misc_feature order(7525..7527,7534..7539,7546..7557,7564..7566, 7579..7581,7645..7647,7651..7656,7777..7779,7783..7785, 7969..7971,7975..7977,7996..8001,8005..8010,8224..8226) /locus_tag="LAC30SC_10930" /note="DNA binding site [nucleotide binding]" /db_xref="CDD:73191" misc_feature order(7525..7527,7534..7536,7546..7548,7645..7647, 7651..7653,7777..7779,7783..7785,8224..8226) /locus_tag="LAC30SC_10930" /note="substrate interaction site [chemical binding]; other site" /db_xref="CDD:73191" gene complement(8335..8814) /locus_tag="LAC30SC_10935" /db_xref="GeneID:10271852" CDS complement(8335..8814) /locus_tag="LAC30SC_10935" /note="COG3727 DNA G:T-mismatch repair endonuclease" /codon_start=1 /transl_table=11 /product="very-short-patch-repair endonuclease" /protein_id="YP_004293190.1" /db_xref="GI:325955716" /db_xref="GeneID:10271852" /translation="MKYKFDTTPEKSRQMSKVHSKNGKDELVLRKLLWHYGVRYRANY KALPGKPDIAITKYKIAVFVDGEFWHGYNWKVKRTRIKRNRDYWIKKIERNINRDSAQ NQELHNMGWTVLRFWYHDVTKNPDYVIQTILYYVRCTEEDPEGTKGSFFRSGGGSLV" misc_feature complement(8452..8784) /locus_tag="LAC30SC_10935" /note="Very Short Patch Repair (Vsr) Endonuclease. Endonucleases in DNA repair that recognize damaged DNA and cleave the phosphodiester backbone. Vsr endonucleases have a common endonuclease topology that has been tailored for recognition of TG mismatches; Region: Vsr; cd00221" /db_xref="CDD:29956" misc_feature complement(order(8461..8463,8512..8514,8521..8523, 8530..8532,8617..8619,8668..8670,8743..8748,8773..8784)) /locus_tag="LAC30SC_10935" /note="additional DNA contacts [nucleotide binding]; other site" /db_xref="CDD:29956" misc_feature complement(order(8530..8532,8761..8763,8770..8772)) /locus_tag="LAC30SC_10935" /note="mismatch recognition site; other site" /db_xref="CDD:29956" misc_feature complement(order(8518..8520,8605..8607,8659..8661, 8737..8739)) /locus_tag="LAC30SC_10935" /note="active site" /db_xref="CDD:29956" misc_feature complement(order(8458..8460,8614..8616)) /locus_tag="LAC30SC_10935" /note="zinc binding site [ion binding]; other site" /db_xref="CDD:29956" misc_feature complement(8608..8613) /locus_tag="LAC30SC_10935" /note="DNA intercalation site [nucleotide binding]; other site" /db_xref="CDD:29956" gene complement(8836..9045) /locus_tag="LAC30SC_10940" /db_xref="GeneID:10271853" CDS complement(8836..9045) /locus_tag="LAC30SC_10940" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_004293191.1" /db_xref="GI:325955717" /db_xref="GeneID:10271853" /translation="MFYVVRWSDLAPEPKSVFVTIDIEMAQDFILKEIDRLITLKSDY DDVVYFGITKIIGGITKCAEIDMHA" gene complement(9190..9594) /locus_tag="LAC30SC_10945" /db_xref="GeneID:10271854" CDS complement(9190..9594) /locus_tag="LAC30SC_10945" /codon_start=1 /transl_table=11 /product="replication-associated protein RepC" /protein_id="YP_004293192.1" /db_xref="GI:325955718" /db_xref="GeneID:10271854" /translation="MAKFEFKKDEKKTTEKKPRPVKKTNISKPQETYDPLKANQKIEQ ELEKNTTKQATSTNGKRPVGRPKTGRKSYQTVRLLKSTVTKINALENALGIATQDETV DQAIDRVINNLTNDEKRGYDLWLDMFKKKENK" gene complement(9596..10483) /locus_tag="LAC30SC_10950" /db_xref="GeneID:10271855" CDS complement(9596..10483) /locus_tag="LAC30SC_10950" /note="COG1192 ATPases involved in chromosome partitioning" /codon_start=1 /transl_table=11 /product="replication-associated protein RepB" /protein_id="YP_004293193.1" /db_xref="GI:325955719" /db_xref="GeneID:10271855" /translation="MATTLLFGNMKGGVGKTTNSVMVAYQLAQMGYKTLVCDLDPQSN ATQLLRRTYGLQHDGEDLKIDKTMMVALNEEDISSAIVNVMDNLYLLPSSEDFKNYPD FLEIKFMPNKEKIEQNPSLLQSEMSHVKEQRIAYFAKQLETINDDYDIIIIDVPPTLS VFTDSAIYATDEVIIVLQTQQRSLDGAETFFEYLQQMYNSYSNVDFDILGVLPVLLKK RSGLDDQILKDAEEDFGDDALFDNIIHHMERLKRYDRTGIADKDLTKSDIHDRRIHYI YSQLTKEIIQRLKDKGGLN" misc_feature complement(9608..10483) /locus_tag="LAC30SC_10950" /note="ATPases involved in chromosome partitioning [Cell division and chromosome partitioning]; Region: Soj; COG1192" /db_xref="CDD:31385" misc_feature complement(<10358..10474) /locus_tag="LAC30SC_10950" /note="ParA and ParB of Caulobacter crescentus belong to a conserved family of bacterial proteins implicated in chromosome segregation. ParB binds to DNA sequences adjacent to the origin of replication and localizes to opposite cell poles shortly following...; Region: ParA; cd02042" /db_xref="CDD:73302" misc_feature complement(10433..10453) /locus_tag="LAC30SC_10950" /note="P-loop; other site" /db_xref="CDD:73302" misc_feature complement(10433..10435) /locus_tag="LAC30SC_10950" /note="Magnesium ion binding site [ion binding]; other site" /db_xref="CDD:73302" misc_feature complement(9845..>10021) /locus_tag="LAC30SC_10950" /note="ParA and ParB of Caulobacter crescentus belong to a conserved family of bacterial proteins implicated in chromosome segregation. ParB binds to DNA sequences adjacent to the origin of replication and localizes to opposite cell poles shortly following...; Region: ParA; cd02042" /db_xref="CDD:73302" gene 10890..11786 /locus_tag="LAC30SC_10955" /db_xref="GeneID:10271856" CDS 10890..11786 /locus_tag="LAC30SC_10955" /note="COG1403 Restriction endonuclease" /codon_start=1 /transl_table=11 /product="HNH nuclease" /protein_id="YP_004293194.1" /db_xref="GI:325955720" /db_xref="GeneID:10271856" /translation="MDISFNSIDLTYHTLDLHLTKNGMLVLGKRQNTKLISEQALKEI IKGAELTLQLYKKYNVVPEAVNREMWNINNQTAKSRITISAERRRKIYEKYDGKCYYC GKDVDDAYQIDHRIPVARGGTNDDDNLVLACAHCNESKNSLSEAGYIINDCDLDRVKK LLKDIKPVMVHTDKVIKEEKDKLDQAMEAADKWRAKAIQKYPEYAELVDYPFLFKEFI QVSSENRKQVVKWCKDSFELAKIGNRKLKADKLTPKYNANPHNEYKIRDYHILEAAMI VAGFKPVDPFKSNQWYKIKYRG" misc_feature 11154..11309 /locus_tag="LAC30SC_10955" /note="HNH nucleases; HNH endonuclease signature which is found in viral, prokaryotic, and eukaryotic proteins. The alignment includes members of the large group of homing endonucleases, yeast intron 1 protein, MutS, as well as bacterial colicins, pyocins...; Region: HNHc; cd00085" /db_xref="CDD:28969" misc_feature <11160..11471 /locus_tag="LAC30SC_10955" /note="CRISPR-associated protein Cas9/Csn1, subtype II/NMEMI; Region: cas_Csn1; TIGR01865" /db_xref="CDD:162567" misc_feature order(11220..11222,11226..11234,11238..11240,11271..11276, 11286..11291,11298..11300) /locus_tag="LAC30SC_10955" /note="active site" /db_xref="CDD:28969" ORIGIN 1 gttcctcgta agcggcactt ggactgtggt agcacgatag ggagatcact aaaatttgaa 61 agaaagacac gtagggcgtg cacgatgcgt ggaaaaaggt gattgaacaa ctgaaatgtt 121 gtttgaagct agtagttcgc tgagatgacc gatcgaccga tcgacatgta gacagtaatt 181 ttttgcatta aatttgcagg gaaagttctg cctcaatttc cctcttccct ctcaacatgc 241 aactaaaaat gctacttttc aataattttt atttaattag taattacttt tatgcatggt 301 gttgcgagtg atataacttt atgttatatt taaatcacat caagaaagag acataaatat 361 tttagaaaag aaattaagca atgatgtatc gcgatttttt taaacgtcga gaaagcatca 421 ttgctttttt tgatgctttc gcaagccatt aatcatatat cttcatagaa acaaaaacta 481 catataagga gagattttta catgaaatac gaattaactg aacaatcagt acagtttact 541 tttggtaaca aacgctacaa gtcaggcttg aagaacaaga cctacgcaaa cgttgataaa 601 gatgccagtg ctgatgcgct tactgttgtt ggtcaagcta ttgcaggctt acaagacgac 661 agtcttgacg atactatttt gatccaaaga cgccgtgttg ttgaatcagc tgaataataa 721 atagaaagaa ggaattaacc tatgactacc acaaccaaat cactcagact taactttaag 781 aatgctaaag ataagtcaac ttcaattact cttgctaatg ctttagaaaa cttgccagca 841 gacaaggtaa aggatgctat gcaaaagatc gcaggttcta atgtctttaa gaaagatgga 901 actgttttat acgtaactcc agtttcagct aactacatcg aacgtactgt tactccagta 961 tttaatgatg aagctgaagc taaggatgct gaataattag cttattagta gaggtctttt 1021 cgaggggaag ggattaagtt cccttcccct ttttttatca aaataaggga attaagaatg 1081 aaggaatacc tagttttaac tggttacgct caagccgtat accgcaaatg tgagctttgc 1141 aaaaaggtga aggatgtcaa ctaccgacta gacgtaaaac acgcagaaag tccggtaatg 1201 ctgactggaa gccttgattt atgcaaggtt tgtgctgcca atttcggcga gataatcaag 1261 aaaggagctt agatgactca agaaattttg aaatatgttc tggagcaggc gtccggagtg 1321 gtcattgcat tgatgttaat catgcggata gagactaagc tagacagctt aacatcagcg 1381 atcatgcgcc tttcggaacc taaaagttca aaataaacac acgaccaatt tttatattta 1441 tgcaaagtaa aaattatgaa aaacttatcg atgaattcat cgaagactta cgcctacagg 1501 ggcgcgcaga gggcaccctg cttgaatatg agatgcgttt aaaggagttc gctgagtttt 1561 tccaactaaa agctttagct ttggatcagg tcaaaaaatc agatttgacc gaatttatgg 1621 aacaaaagct atcaaactgt ctgaaagttt cgacaatcag aggtaaactc agtacctttc 1681 gtgtattttg cttatgggcg gtaaataaaa attatttatc cgaagtaatt atcagtccgc 1741 aagattatcc gaagaacacc aatgtaaagc ggataagacg attatccgat gaaaatttga 1801 ccattttcaa acattacatt gatactttgc aaccgaatgc gcgcgcagca ttttatttgc 1861 taattggatc cggttgtcga gtagcagaag cggctaatct caaggcagaa gatgtaactt 1921 tacgcggtaa atccgtgtat atagacattc ggaatgctaa atggggaagc gatagaaaca 1981 tacctattgt cgatgccgat gctgccaaaa ttgtttggaa gtatcgcacg gaagttgaag 2041 tcgataatag gccactcttt cgcctttcca agcggacatt gcagaattat gctactaatt 2101 ttgccaagag taccggaata aatttttatt gtcacttact acggcatact tttgccggcc 2161 ttttaaccga aaaaggcgtg ccgctgacca cagtccaata cctgttggga cataaaagtt 2221 tgggcatgac ggcccactac gcgcaatctg cgcgagttga tattacagat atcacaccag 2281 aaattaattt atagatttag aaagatttta aggagaataa gagaatgaac gatattaagt 2341 tattaggtag attgacagga aacccagaat tgaagcaaag cgcaaacggt cgcgtttatg 2401 catggtttac ggtagctgtg ccacgtcaaa ataataggga cgaagctgac ttcattcgtt 2461 gtgttgcctt tgataagctt gctcaagcta ttgctcaaca ttgtaagaag ggccgtcaag 2521 tgttagcaca tggccgttta caagtttcta catcaatgga taacactact caacaaacta 2581 gatatcatca tactgttgtt gtctcaaaca ctcacttttt gcacgatcct aaccgtgcaa 2641 cagtagctgc ttaagccagc tctgtctttt cttgctgtca acggtaaccg gagaggctga 2701 aagccttgag gattaaggag ttgacagcgg tctattgcta ctcaagctag tactcagcaa 2761 aacaaaaatt gttttgcctg cctagcggcg agcggaagaa aatcatctcc cgcttgtatc 2821 aaccccccat tactaatagg ggggtagaca atacgcagaa ggatgtatga acatggaaaa 2881 tactgcttta actgtatcta tcgactggtt ggaatttaca attctaaatt ctcagttgca 2941 tgaagtcatg aagacgatgg agctgaagtg ggacgatttt agcaaattgt caaaaggaaa 3001 gtttggttac aacaaccaaa tcaaatggtc cgatgggagc atcttcatca tgtttactgc 3061 caaggatgaa gacgtgaacg aaagcacatt gatcaatcca aagtcgggcg tgcatgtgat 3121 gattacagga aaaggatgtc gtcaatactc cgttaatcat gatttaattt cgcttatcaa 3181 gcgtctgtac tcactaccta gggttaattt ttcacgaatc gaccttgcaa ttgacgattt 3241 tgaatcaaag atcattaact atgatcggat tcatgaggca gcaattaacg gacacttcac 3301 ctcacgctgg agtaagtggg acgaaatcaa ttcaagacaa acttccgaca atcattttct 3361 tggacggacg atgtattttg gatcacaggt atctgaaatc ttttgccgaa tttacgataa 3421 aacactggaa agaaaagcta aatcagatga aaccgagatc ccaaaaaatt ggacgcgtct 3481 tgaaatggtt tatcgcaagg aaagagcgac aaaactcgtc gattttttag taaacaagaa 3541 tatgccgatc ggttatgtgc tgagaggaac gctcaaacat tacttacgat ttattttgcc 3601 atcgactgat cgaaacaaag ccagatggcg taccacagca tggtgggaag agcttttatc 3661 tagcgttgat cgcttgcaac ttactgtaaa gaaagaagca agaacgattg atgacatgac 3721 agcttggatc gaccaacaaa tatctcctac attgagtgca attttaaaag cacaagaagg 3781 cgatcttggc tggttgagat caattttagc taaaggatct aaacgactta ctcagaaaca 3841 caaagatgca attaatcagt atttgaaagg aatgaccgta tgactagtac atcacaaagc 3901 tatcctaggc tggctttctg ttcagcaatc accccagctg atccagccca ttatctgaac 3961 ctgcgtaaag caggaattga tacagcgagc atttgcctgc atgtatcagg atttgaatat 4021 tataagtttg cgacgattca cacccagtta gcacgtaaag cagacttagt aacccacgca 4081 tttatgatca ctgatttact tgatccattt gcggatgtca caacattttt taaacgattt 4141 aaccagttag gttatgattc taacgctaag attacagtgt gggttaacgg tgataaatac 4201 gttgctgaca gggaatccaa gatcattgag attattgatc tcatcgcaaa atgtcataac 4261 cgtgagaaca ttgatcttgc attttttaaa cgtgatatcg acgataagtt atacgattta 4321 tccaagatgc cgcaaatgat taatctaacg attatcaatt gcgagggcac aggcgctggt 4381 attgatatag ctggtacatg gatttatacc atggacttct gcaacgaagt tcaagttcta 4441 ggctacgatt actacggtta ctacactgat gatgctggat accagctatc gttggttgat 4501 accgactacg tagtgcaaga aggagataca tggtattcaa tttctagaag acacggtata 4561 ccgctaaatg atctgctagc gttaaacaga gcaatagaaa ctgaaagagt atttgccgga 4621 caagttgtcc ggattgcata attttttatc aggtggggct tcgttcggcg gggtcggtgc 4681 tctatctctc aagggctctc cagccgctaa cgcgtcttcc gtttagccct tatcgagcta 4741 agtcgcacct cagcctcact gcgccccacc tgcaaaatca aacaaatata aaccaaatca 4801 aaaattatta ttaatataaa ttcacatttt tcttcatacg aaatcaaagg cggatcaaat 4861 aatgatctgc tttttttacc cctcaacaga ccacacaaaa aaattgcaag tctataaatt 4921 tattttttta cttgctagac ttatgccaaa actaaaagca aatcagaaat aacggaggtt 4981 caaattatga aagtatatcc ctggttagac agtatagcag cgcctgtagt aggcaccaaa 5041 tacgcgttag gtgagggctg cgaattatta aataaactag atgataccgg ttgggtaatt 5101 gatggtacag aaagctccta tatgctagaa gaggcttatg tatatgaaca tattgcagag 5161 ggtatgttac taccagaacc tgagaatcct gtagacccta aagcagttgc ggtttatctt 5221 cgatttgtgg caactaagaa gagtatgaga cctcataaga tggctgtccg tattggatac 5281 ctacctgaag agtctagata caagaagtgt attaaaaaag caaccatggt taaaattcat 5341 tgtagggaca tgatctttgg cactgatcca gctaggtact tcgatgctga ggttgtcgac 5401 gtaccactta aattgacgtc taaagagtat gaatgcatgg ctatggattt atatctagaa 5461 taattaaaaa gcgctctatc gttgttatag agcgcttttt gtgtgcaatg atatgccagt 5521 gtggcagggt taggttacat ggtgaatacc gctcatgatc tctgttggtt tgacatggct 5581 tgcatgatcg gaaaggagag accgcagggc tcgacccgat caggtgagac atggcttccc 5641 gcgcaatcgt tgtttgcggt attcaccatg taacctaaga accaactgtt agcatcaccc 5701 tcgtttagtg atacaatgaa tatatcgtaa tattgattta tgctatgaat ctcggagtcg 5761 cattatgcgg ctcttttttg ttcttttcag ctataccaat ccggctgcgg ataagtacca 5821 ttatgtaaag tgacttcagc cttactctcg caagggcaaa aatcggcaaa aaacggtgct 5881 tgtgaaattt cagccgtata caaaaagaga accccggtat tacgggattc tcttttctgt 5941 tttttaaaaa cattcacaat tataccaatt tgtcttatac attccaaggt gctataaatg 6001 acctaatact ctctggagta gatacagatt caatatcgaa tttcatagaa ttttccactt 6061 tactacttgc attatgtatc cgtaaagaga atgaccaacc ttcatcacaa gtaacaataa 6121 tagtattatc ggattggttt attgcatttg tcttcttatc tagtaccttt ttaaatccaa 6181 tatgatagat cactgatggc aatttaggct tttcgattga aaacatagat ctatgatctt 6241 tgtatctctc gttcaaagaa tcatgtaagt ttacaggggt tattaatgtt atatgctttt 6301 tctctactga tatgacttta tagaaatctt tctctcctat aaggtaatta accaatgcag 6361 gagctatttc accagggtac tcctcatcta acctggtcaa ttcatccata aaagccttca 6421 atagaggttt atatacatca tcgactttat ctaccttgtc gctcacttta tcccattttt 6481 ccttaacata tggctttaaa ttagcaaaaa ttctaccaac ttcgtccata tattcagaac 6541 tacattgttt acctaaccat tcttttccaa aatcaatcgt aggtgaaatt cgcgagtgct 6601 tcaaagcaaa gtgattatgc ttacatgaga cccctaattc ccagctcttc ctcttactat 6661 catttcttat acaaagtatg tctctgacat cacctttttg accattagaa tctgtttgta 6721 gtgttagctt taatacacta ttatcagcat aatgatccaa tttaggctct aataagttga 6781 ttaatttaat ggcagcttcg cctgcaagaa gatcattttt accatggtca cttaatgcat 6841 caaaatatga tttagcagtt tctaactggg gagattctat tatctctact gggacttcct 6901 tttccttata aaagtcataa attgctagca aacatgcgta ttcaaacgcc ttaccgcttt 6961 ctgtatttgc caatagccct accttcttta atataaaata ctggcctttc cagttcatta 7021 atgctacaat gctgttttaa ggacatacta gcaaaaatac cggagggagt acagttgaag 7081 aaattcgaat catctttaac tttttcagcg gagaaggtta agaaaatcct attcgccaaa 7141 attgaagaag aaaaagagaa aatagagaat aataaaacag aagaaaacgt aaagctagat 7201 aaagaactat atgataaggc ttccgctctg tatgatgata gcaaaattaa cgtattatct 7261 ctttttagtg gtgcaggcgg tttagattta ggtgtagaaa tagctgcagc atcagcaaaa 7321 catggagtag ataaaaccta tacagcattt caaaatagaa gagaattatc caaattatta 7381 gactccacaa acgtcatata ttccaatgat ttatttgctt ccgctaatgc aacttataaa 7441 gacaacttct caggtaatta tgtaaaagat actcgtgacg ttagaaaagt aatctctttc 7501 cccaaagctg atctagttct tggcggattc ccttgccctg gatttagtgt agctgggcct 7561 cgactcttgg atgatccacg taatttctta tacatccact acatcagagc cttaatggat 7621 tccactccaa aaatctttat tgcagaaaat gtaaaaggtc ttatgaccat ggctggtggt 7681 aaagtcctat ctcaaatgaa ggaagacttc tcagcagcag gctataaagt aactgctaac 7741 ctggttaatg ctaaggatta tggtgtacct caatcacgtg aacgtgtatt tctagtaggc 7801 gttagaaatg atatcgctga taaattccaa tacactgtac ctgaacctac taatggacct 7861 gacactgaca agccatacat tactttaaaa gaagctatct ctgatttacc tgaacatcca 7921 gcagattact tcgaaggtac ttatagttcc atctacatgt cccgtaacag aaaaaagtca 7981 tgggacgatg tatcatttac tattcaagct tcagcaagac aagcacctca atatcccggc 8041 ggaaagccta tggtaaagat tggaagagat aaatgggaat tccaaggtga tttaaacaga 8101 agattatctg taaaagaatg tgctagaatt caaacattcc cagattggtt tgaattctcg 8161 gatggcggta aagataatgt aagtttagcc aatcgcctca atgaaaaata taagcaaatc 8221 ggtaatgctg ttcctgtctt acttgccgaa aagattgcgc gacctgtaat gcaattcctt 8281 gaaaattata aatagaaata aaaacctatg actcaaagcc ataggttttt tgaattacac 8341 tagactaccc ccacctgatc taaaaaaact acctttcgta ccttcggggt cttcctccgt 8401 gcaacggaca taatataaaa ttgtttgaat cacgtagtcg ggatttttag tcacatcatg 8461 ataccaaaaa cgtaaaaccg tccaccccat attatgaagt tcttgatttt gtgcggaatc 8521 cctatttata ttacgttcta ttttcttaat ccaataatct ctattccgct taatccgggt 8581 tcgtttaact ttccagttat agccatgcca aaactcacca tcaacgaaaa ccgcaatctt 8641 atacttagta attgcaatat caggcttgcc tggtaaagcc ttgtaattag ctctgtatct 8701 tacgccatag tgccataaaa gcttcctcag cactaactca tccttaccat tcttcgaatg 8761 aaccttggac atctgtcggc tcttctcagg agttgtatca aatttatatt tcatataatc 8821 aactccaatt caaaatcatg cgtgcatatc aatctcagca catttcgtta ttccgccgat 8881 tattttagtt atgccaaagt aaacaacatc atcataatca ctcttaagcg taatcagtcg 8941 atcgatttct tttaaaataa aatcttgtgc catttctata tcaattgtca caaaaacgct 9001 cttcggttct ggcgctaaat cactccatcg cacaacgtaa aacatctaat catcctcttt 9061 aatcagaatc gcgggtaaac ctttgccacc aatgctttta attggtcctt ttgataaaat 9121 ttatctttct cttttgttga acttgaactc tcattgctct tctcagcacc ataaaaaagc 9181 ttttgctgat tatttatttt ctttcttctt aaacatatca agccaaagat cgtaaccgcg 9241 cttttcatcg ttggttaaat tgttgatcac tctatcgata gcctgatcta cggtttcatc 9301 ttgggtagct atacccaatg cattttctaa agcattgatc ttggttacag tagatttaag 9361 caaacgtact gtttgatatg acttacgacc agtctttgga cgaccaactg gacgcttgcc 9421 gttagttgat gttgcttgtt ttgttgtatt cttttcaagc tcttgttcta ttttttgatt 9481 agcttttaat ggatcataag tttcttgagg tttagaaata tttgttttct taactggacg 9541 tggttttttt tcagtggttt tcttttcgtc ttttttgaat tcaaattttg ccattttagt 9601 tcaaaccgcc tttatctttt aaacgttgga taatttcttt ggttaattgt gagtagatgt 9661 agtgaatgcg tctgtcatgg atatcagact tagttaaatc tttgtcagca atcccagtac 9721 gatcgtatct cttcaatctt tccatgtggt gaatgatatt gtcgaataat gcatcatcgc 9781 cgaagtcttc ttctgcatct ttcaatattt gatcatctaa tccggaacgc ttcttaagta 9841 aaactggaag tacacctaag atatcaaaat caacgttgga atatgagtta tacatttgtt 9901 gtaaatattc aaagaatgtt tcagcaccat ccaatgatct ttgttgtgtt tgtaaaacaa 9961 tgattacctc atcggtggca taaattgctg aatcagtgaa tacagataat gttggcggaa 10021 catcaataat gataatgtca tagtcgtcat taatagtttc caattgcttt gcaaaatatg 10081 caattctttg ttcttttaca tgtgacattt cagattgcaa taagcttgga ttttgttcga 10141 ttttttcctt gtttggcata aatttaattt caagaaaatc tggataattc ttgaagtctt 10201 cactggatgg taataagtaa agattgtcca ttacgttaac gattgcagat gaaatatctt 10261 cttcattcaa tgcaaccatc atagttttat caatcttgag atcttcgcca tcatgttgca 10321 agccataagt acgacgtaat aattgtgttg cgtttgattg agggtccaag tcacatacaa 10381 gggtcttata tcccatttga gcaagttggt atgcaaccat tacggaatta gtagttttac 10441 ctactccgcc tttcatattt ccaaataata atgttgtagc catattcgta cgctttcttt 10501 aatgatagtg ctttttttat tattcataat tagtttacta caagaaatta tggaaggcta 10561 taattttaag tggtaaataa ttaattgtgg tgaattattt attgtagtag atatatatgt 10621 gtagtaggtt atttattgtg gtaagtatat aactgtagta aaaaatattt gcttctataa 10681 aaataaaagg gaccgtttta aaaattcaaa acgatctctt ttaattgata gctatttatt 10741 tactacagtt atatattgtg gtgaattata tatttactac aattaattat tttttagtat 10801 ttgtaaactt gtagcaaata atgataaagt ttgttgtaaa gatgaacatg ataatttaga 10861 cattaaaaaa ataactaaga gtaaacaaga tggatatatc atttaattct attgatttaa 10921 cgtatcatac attagattta catttaacta aaaatggaat gctagtatta ggtaaacgcc 10981 agaatactaa gcttatatca gagcaagctc taaaggagat tattaaaggt gctgagttaa 11041 ccttacagtt atataaaaaa tataatgtag tgcccgaagc agttaatcgt gaaatgtgga 11101 atattaataa tcagacggca aaatctagaa ttactatctc tgcagaacga cgtagaaaaa 11161 tctatgaaaa atatgatggc aaatgttatt actgtggtaa ggacgttgat gacgcctatc 11221 aaatagatca ccgaatacct gttgctagag gtggaacaaa tgatgatgat aatttagtat 11281 tagcttgtgc tcattgtaat gagagcaaaa atagtttaag cgaagctgga tatattatta 11341 atgattgtga tcttgatagg gtaaagaaac ttttgaagga tatcaagcct gttatggttc 11401 acaccgataa agtaataaaa gaagaaaagg ataaattaga tcaagcaatg gaagcagctg 11461 ataagtggag agccaaggcc atccagaagt atcctgaata tgctgaattg gttgattatc 11521 cttttttatt taaagagttt atccaagttt cgtctgagaa tagaaagcaa gtggtgaaat 11581 ggtgtaaaga tagttttgaa cttgctaaga ttggaaatag aaaacttaag gcagataaat 11641 taactccaaa atataatgcc aatcctcata atgaatacaa aattcgagat tatcatatat 11701 tagaagctgc aatgattgta gctggcttta aaccagtaga cccattcaaa agtaatcagt 11761 ggtataagat taaatatcgt ggttaagata aaaaggcatc catattggat gctcttttta 11821 ttaaaaattc aaaaattatt ttttgataat tgattgtatt tatctactac atttatatat 11881 tgtggtaaat aaataatata ccacaactta ttaatgaaac taaaaataga acattgctat 11941 atcaacgttt ctaagcatag tacattatat attgtagtac attatatatt gtggtaaata 12001 aataatatac cacaacaaat tcaaatttca ataaattgcg atggtattaa tgaatttatg 12061 cctcttgtaa ttgctttcta aatatgtata ctagagtcga atgagttaaa aaattaaata 12121 agaacataaa aaaatcgaat ggtattgacg taccattcga cctgatcgta atcacagcaa 12181 aagcatacaa ttacgaaatt aaaaagcttc ttgatattat gttaccacta gggcaacaaa 12241 aatcaagtat gttgattgct gtaataagcg atttattatg cggtagtagc ataaaccgat 12301 atcaacatgc ttgaaaaaaa gctgtgcttg ccgcagcttg gaacaaaagg taacgtccat 12361 atatgactca agctactata atatatggaa ggtaatcggg taaaagtcag aagcccggac 12421 aaatctttga tttgaagtct aaggttcatc gtgagatgta actatggcga aaactgatgt 12481 gtgtaagtcg atggcttgag aaccattgca atggctgaac ctcgccaaga tgtacgactg 12541 gaggcaggct cacatgtgat gtgagtaa //