LOCUS       NC_012634              12377 bp    DNA     circular BCT 29-APR-2009
DEFINITION  Rickettsia africae ESF-5 plasmid pRAF, complete sequence.
ACCESSION   NC_012634
VERSION     NC_012634.1  GI:228478451
DBLINK      Project:18269
KEYWORDS    .
SOURCE      Rickettsia africae ESF-5
  ORGANISM  Rickettsia africae ESF-5
            Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
            Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
REFERENCE   1  (bases 1 to 12377)
  AUTHORS   Fournier,P.E., El Karkouri,K., Leroy,Q., Robert,C., Giumelli,B.,
            Renesto,P., Socolovschi,C., Parola,P., Audic,S. and Raoult,D.
  TITLE     Analysis of the Rickettsia africae genome reveals that virulence
            acquisition in Rickettsia species may be explained by genome
            reduction
  JOURNAL   BMC Genomics 10 (1), 166 (2009)
   PUBMED   19379498
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12377)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2009) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 12377)
  AUTHORS   Fournier,P.-E.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2009) Unite des Rickettsies, Faculte de Medecine,
            27 Blvd Jean Moulin, Marseille cedex5 13385, France
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CP001613.
            Source DNA is available from Dr Pierre-Edouard Fournier,
            Pierre-Edouard.Fournier@medecine.univ-mrs.fr.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12377
                     /organism="Rickettsia africae ESF-5"
                     /mol_type="genomic DNA"
                     /strain="ESF-5"
                     /host="Amblyomma variegatum (cattle tick)"
                     /db_xref="taxon:347255"
                     /plasmid="pRAF"
                     /country="Ethiopia"
     gene            1..2223
                     /locus_tag="RAF_pORF1260"
                     /db_xref="GeneID:7803247"
     CDS             1..2223
                     /locus_tag="RAF_pORF1260"
                     /codon_start=1
                     /transl_table=11
                     /product="Unknown (DnaA-like)"
                     /protein_id="YP_002845762.1"
                     /db_xref="GI:228478452"
                     /db_xref="GeneID:7803247"
                     /translation="MKNSSLCFGGLGVNNTQYTSLDSSMSSSLDSLLSPRDILFFLQI
                     VSLYQLELSKGNDSITYPSLKLAKLLGVDASCDTAYLTSSAHHSVNSLTDSNMRATIL
                     KMTAKLESLGLLKVFRRKRSNGMDLANKLVPVLPDRLYSVIKDSPTNLKVSNSSKLDR
                     ESNLEHILRIKLFVPIELEFMKSLFKNNLPSKYKLFFLNCIMSAYRNFMQNSNGSNML
                     SFSATSHELIQQNNMSKRTLFNIFKYIKQQGDSFFLKVEHKYTKSDDMDCNRYDKSIF
                     NISINPLVFPISQIRKSQTEEITEFNHEELQGNNSLAFSDTYRDFREGVQKKQPISAK
                     KAALNNKEEIIKNKYGKNIDYIDANSENSSIPCSSSTLTSEQNYKKEFFKDKQSLSSS
                     ESYNNSSFLNDSSSRNNKNISEQKEQWAELRHFYPLSEKDIDTLNFRAHREFSNNFVN
                     QLLLKLYIKYPEKRFKNKFTFLSYMVQILKNEKHQGPLVNHTSFRFSCNIETIEQNLL
                     KYEKYLNQIESSFDISKEMQVRKKIAGRFNTNVAYEILTQVEFKTNHNNSFITAIIPK
                     RLDLGGIQTGSLSERQIEILSEQLEAVYGINGYYVTVVGDVGSAEDSSGKGERKEEIE
                     KQYSKIKESDIISNIKDIGILDIGKELDKIEENTVWNQIRKGLREELGEAIDAVWFSK
                     AEATEDKEKGMLILTMPTRFMADWVKNNYSHVLKRLSVGCGINRVEYEYEQSNKLLLT"
     gene            2263..2964
                     /locus_tag="RAF_pORF1261"
                     /db_xref="GeneID:7803237"
     CDS             2263..2964
                     /locus_tag="RAF_pORF1261"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_002845763.1"
                     /db_xref="GI:228478453"
                     /db_xref="GeneID:7803237"
                     /translation="MTDIKSILKGINTDDTSAKNMIMINELRDITTDCLKKLQVIRKD
                     LTSAYIVSQHIIINEDDKYEFKPEKEVRAENKQHLDLDKVQREEEKILKDILDTISSQ
                     KTVEDKEQNNQKSALKTDTSNSTKNIFVKIKNLIKKLISSLYPKEKEINVSSETSNNH
                     VDLMEQNVKNIKIYNKELDKRVEALKNIDLLQEKISQYKERAVDPKEYNKKKDFVTEV
                     IQRKQHVGQSKINSR"
     gene            3122..3706
                     /locus_tag="RAF_pORF1262"
                     /db_xref="GeneID:7803238"
     CDS             3122..3706
                     /locus_tag="RAF_pORF1262"
                     /codon_start=1
                     /transl_table=11
                     /product="Site-specific recombinase, DNA invertase
                     Pin-like protein"
                     /protein_id="YP_002845764.1"
                     /db_xref="GI:228478454"
                     /db_xref="GeneID:7803238"
                     /translation="MLIGYARVSTLDQNPNHQIDALNEAGCKKIFTEKISGASKQRTQ
                     LQAALDYMREGDTLVVWKLSRLARSLTQIISTLKILEEKKISLHVITQSIDTSTAEGR
                     LFFHMNAAFDQFQREIIVENTKTGLKSARRNGRIGGRPKFMSDEKMRTAKAMLKDNEN
                     YPFISDIIKTLDIGRTTFYRYFPPKSIEQLRTRQ"
     gene            complement(3862..4503)
                     /locus_tag="RAF_pORF1263"
                     /db_xref="GeneID:7803239"
     CDS             complement(3862..4503)
                     /locus_tag="RAF_pORF1263"
                     /codon_start=1
                     /transl_table=11
                     /product="Transposase and inactivated derivative"
                     /protein_id="YP_002845765.1"
                     /db_xref="GI:228478455"
                     /db_xref="GeneID:7803239"
                     /translation="MRQALICPIVVYANDKPYNAPRSFWELFEDSKTAKDMMGDEYLL
                     VDLQKQSDDEIEKKKHLGMMEYMLKHIKARDILNLWQSLLEKFESSIEIDKENGYIYI
                     KWLLWYSDAKVSEDKQVELASIIAKHLKKEDQEELMRTIADKYIDEGVQKGMVQGMQI
                     GEARGMQIGEAKRTMEVAKNMLSNNYSIPEVSRITGLSISELNTINLTNKKDS"
     gene            complement(4676..5761)
                     /locus_tag="RAF_pORF1264"
                     /db_xref="GeneID:7803240"
     CDS             complement(4676..5761)
                     /locus_tag="RAF_pORF1264"
                     /codon_start=1
                     /transl_table=11
                     /product="Transposase and inactivated derivative"
                     /protein_id="YP_002845766.1"
                     /db_xref="GI:228478456"
                     /db_xref="GeneID:7803240"
                     /translation="MNNKNTPKDSSELSIADTKESNRNHEQVDSNSSNTSERPRHDEL
                     FKKVMSEPVAAREFLEHYLPVTFKNKINLNSIKIEKESFVTEDLRKRLSDVVYSVSLK
                     NDNIKDSTTEKSVHNDKAYVYVLIEHQSSSDYWIAFRLWQYMLLLCERHKDANNNKSS
                     VTKEKDNKLPLICPIVVYANDKPYNAPRSFWELFEDSKTAKDMMGDEYLLVDLQKQSD
                     DEIEKKKHLGMMEYMLKHIKARDILNLWQSLLEKFESSIEIDKENGYIYIKWLLWYSD
                     AKVSEDKQVELASIIAKHLKKEDQEELMRTIADKYIDEGVQKGMVQGMQIGEARGMQI
                     ASSIAMQILRIDGKIDPRNNTDSLFLI"
     gene            complement(5805..6203)
                     /locus_tag="RAF_pORF1265"
                     /db_xref="GeneID:7803241"
     CDS             complement(5805..6203)
                     /locus_tag="RAF_pORF1265"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_002845767.1"
                     /db_xref="GI:228478457"
                     /db_xref="GeneID:7803241"
                     /translation="MSNVINFPNEKERITFKRNIANTKRYNIFKSILQLVSKAIYDTL
                     FTIRLTIATTLHYSFCVFLSILNSMNVFLFFFLGLCCIINYFHLGQHFVSPTNYVTPV
                     FIGFWILSCSAQHLVSLIQEHLPFHRICRV"
     gene            complement(6236..6535)
                     /locus_tag="RAF_pORF1266"
                     /db_xref="GeneID:7803242"
     CDS             complement(6236..6535)
                     /locus_tag="RAF_pORF1266"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_002845768.1"
                     /db_xref="GI:228478458"
                     /db_xref="GeneID:7803242"
                     /translation="MATKPLSTSTKIANLAKVKQQRIQKIEAELNVQLTSLLTKRKEE
                     IFNIFNKFSAVDIDDKLLIGFLKFVTNKDNKDHPIIKEFLNIANKTRLLKRKGNN"
     gene            complement(7255..7626)
                     /locus_tag="RAF_pORF1267"
                     /db_xref="GeneID:7803243"
     CDS             complement(7255..7626)
                     /locus_tag="RAF_pORF1267"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_002845769.1"
                     /db_xref="GI:228478459"
                     /db_xref="GeneID:7803243"
                     /translation="MYGLVRDERNITWLEVAGTSHYRNHDFKEAIESFDRMFEVLKHE
                     KLGIPSMQKVIKAISCIDVILSVIGVNLFSIELEYKALKTKCKGLILLEEYEEIAGIH
                     LRLNALNESLKGSFVELKEVS"
     gene            complement(7776..10421)
                     /gene="sca12"
                     /locus_tag="RAF_pORF1268"
                     /db_xref="GeneID:7803244"
     CDS             complement(7776..10421)
                     /gene="sca12"
                     /locus_tag="RAF_pORF1268"
                     /codon_start=1
                     /transl_table=11
                     /product="Cell surface antigen Sca12"
                     /protein_id="YP_002845770.1"
                     /db_xref="GI:228478460"
                     /db_xref="GeneID:7803244"
                     /translation="MKLSYIHGFGNKGNIFEAKGISAESLSHQANLNVTPKYLTPDYF
                     LPSSSNAHYYRKKYKGNLNLNFKSKFRKISRVASVMLLSLGFIATTLEVKAQPVIEAN
                     QSFLFGKGSAGISTNVPLPPIMPQSLPPAPPIPPVLPAMPDFISGANIAGKADNRSAL
                     FESIRNFGKNILKKVKREPKPKVISSDGEDLMSQLHNTLERRRNGIAGEKNGAKPKKV
                     VKQLPKLTSEEQARKKAEIARERRRAEVEAVKARIEQKAKERGENREGVVNGIDPALH
                     HNIVSDLRSEIALLREQLKNQGINNLAGTLGGGAEEPIYSKPIDTISMASSVTEYSEP
                     WQDPDTEIGEAFETEFSMQKQIEPTAPPLSVLSSLEDLHISEDASLSEEERVMKMLDE
                     AIEDSGYSGSSCGGVDDWSGEDSIADTDGYDTVCTSSGSEYSEDSSDEDGIPFSPPAT
                     EEKKAELDIIREFQVSEQSSAVASNASINEANIAADSNESEEASQEESTTASVFEILD
                     EVDEKDSVSKDISTNLGSTASTEPQEQATSMSQEELRKKAEATSVMQNQARKMSKISS
                     KQIRDRIFARDIVTAAVASGDDDADDASEKTIGYSIWSSGTLGINKQKSKADSNGYSS
                     KIAGGTIGADINLENDLLLGASFSRFNSRVKYQDQGGSSDLCSSGTSTRAKYDTHIFS
                     LYGSRPVSRNMNVAIIGSAGHSKGKKVRSKLMSFEPHLNYKVEMPREVTLIPHIGLRY
                     EYEKVSRHKEQIASNLSIERSKKSYQALNGEIGSRVIFAPIKLKGSTPSTISITPTAH
                     FSVERRIGSRGKSNSFTLTYYEAQQTIGTGIVSVNAQNERTSLNAGIGLIANHKNIKL
                     ELLYDQQRQKRFKSHQGVLKLKVSL"
     gene            10574..11047
                     /locus_tag="RAF_pORF1269"
                     /db_xref="GeneID:7803245"
     CDS             10574..11047
                     /locus_tag="RAF_pORF1269"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_002845771.1"
                     /db_xref="GI:228478461"
                     /db_xref="GeneID:7803245"
                     /translation="MSITISIQKFLKVKFYEHKILRKDFINESGIKRSSISELLNGIP
                     VSPSLVTILKIANYFNCSIDEILGRVDYIQDDNTKYIKISPLQMSNNTKDFIVAMLNE
                     NNISSHQLAASCNIGTDTINYFIKKHDAKKNLSIRTIYAVANFFNISIDKIVGRI"
     gene            complement(11231..11902)
                     /gene="parA2"
                     /locus_tag="RAF_pORF1270"
                     /db_xref="GeneID:7803246"
     CDS             complement(11231..11902)
                     /gene="parA2"
                     /locus_tag="RAF_pORF1270"
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid stability protein ParA"
                     /protein_id="YP_002845772.1"
                     /db_xref="GI:228478462"
                     /db_xref="GeneID:7803246"
                     /translation="MSVITIAANKGGVGKTTITALIASNLAKKKKKVYLIDLDPQQSL
                     KQWWQKRENEDISLVDIKYNQLKDALIKINTKDSYVIIDTPPSHLKIIEQAILVADYI
                     LIPCRPSPLDIEAIGETLTIIEEHKKNFTFILNSSVSGTQITEQTILLLSKNGQIAPS
                     PIRQRIIYAIAMLDGRTALEMYNKAAISEINELCNFIEGEIKSNEKKTTKSRNIRGNK
                     KTKAE"
ORIGIN      
        1 atgaaaaata gttctctttg tttcggtggt ttaggagtaa ataatacaca atatacttct
       61 ttagactctt caatgtcttc ctctttggac tctttgttat cgccaaggga tatattattt
      121 ttcttacaga ttgtctcgtt gtatcagtta gaactaagca agggtaatga ttcgattacc
      181 tatccatcat taaaacttgc taaactgctt ggtgttgatg cttcttgtga tactgcttac
      241 cttacttcta gtgctcatca ttctgttaat tctttaactg actctaatat gagagctact
      301 attttaaaga tgacagctaa gcttgagtct ttaggactgc tgaaggtctt tagaagaaaa
      361 agaagcaatg gtatggattt agctaataag ttagttcctg tgcttcctga taggttgtac
      421 tcggtaatta aagactcacc tactaactta aaagtaagta atagttctaa attagatcgt
      481 gagtctaact tagaacatat cttaagaatt aagttattcg ttcctattga gcttgaattt
      541 atgaaaagct tatttaaaaa taatttacca tctaagtata aactattctt cttaaactgt
      601 attatgtcag cttataggaa ttttatgcaa aactccaatg gctctaacat gctttctttt
      661 tctgccacat ctcatgaatt aatacaacaa aataacatga gtaaaagaac tctgtttaat
      721 atattcaaat atatcaaaca acaaggtgat agcttctttt taaaagtgga gcataaatat
      781 actaaatctg atgatatgga ttgtaataga tatgacaaat ctatttttaa tatatctatt
      841 aatcctttag tatttcctat ttctcaaatt aggaagtctc aaacagaaga aattacagaa
      901 tttaatcatg aagagttaca gggtaataac tctcttgctt tttctgatac ctatagggat
      961 tttagagagg gagtgcaaaa aaagcagcct atcagtgcaa aaaaagcagc ccttaataat
     1021 aaagaagaaa taataaagaa taagtatggt aaaaatatag attatataga tgcaaattca
     1081 gaaaattcat ctataccttg ctcaagttcc acattaactt ctgagcaaaa ctacaaaaaa
     1141 gagtttttta aagacaagca aagcttgtct tcatctgaat cttataacaa ttcttctttt
     1201 ttaaatgatt ctagtagtag aaacaacaaa aacatatctg agcaaaaaga acaatgggca
     1261 gaactaagac atttctaccc actctcagaa aaagatatag atacacttaa ctttagagca
     1321 catagagaat tcagtaataa ttttgtgaat cagttattac tgaagctcta catcaaatat
     1381 cctgagaaaa gatttaagaa caaatttacg tttcttagct atatggttca gatactaaag
     1441 aacgagaagc atcaagggcc tttagtaaat catacaagct ttagatttag ctgtaacata
     1501 gaaacgatcg agcaaaactt acttaaatat gagaaatacc taaaccaaat agaaagctca
     1561 tttgatatca gcaaagaaat gcaggtaagg aagaaaatag caggaagatt taatactaat
     1621 gtggcatacg agatactaac gcaggtagaa ttcaaaacaa atcataataa tagctttata
     1681 acggcaataa tcccaaaacg actagattta ggggggatac aaacaggaag tttaagcgag
     1741 cggcaaatag agatattaag cgagcaatta gaagcagtat atggtatcaa tgggtattat
     1801 gtgacagtgg tcggagatgt aggatctgct gaagatagca gtgggaaagg tgaaagaaaa
     1861 gaagagatag agaagcaata ttctaaaatc aaagaatctg atattatttc taatatcaaa
     1921 gatattggaa ttttagatat tggtaaggaa ttagataaaa tagaagaaaa cacagtatgg
     1981 aatcaaatca gaaagggatt gagagaagag ttaggtgagg ctatagatgc ggtttggttt
     2041 tctaaagcag aagctactga ggataaggaa aagggtatgc taatcctaac aatgccaaca
     2101 aggtttatgg cggattgggt taaaaataat tattcacatg tattaaaaag acttagtgta
     2161 ggatgtggaa ttaatagggt ggagtatgaa tatgaacaaa gcaataaact attattaaca
     2221 taaattttct gttaaactag ataaataaca tatgaggttt ttatgacaga tatcaagagt
     2281 atacttaaag gtattaacac tgatgatacc agtgcaaaga atatgataat gataaatgag
     2341 ttaagagata taacgacaga ttgtcttaag aagctacaag taattaggaa agatttaaca
     2401 tctgcgtaca tagtaagtca gcatattatc attaacgagg atgataaata tgaatttaag
     2461 cctgaaaagg aagtcagggc agaaaataaa caacacttag atcttgataa ggtgcagagg
     2521 gaggaagaaa aaatattaaa agatattctt gataccatat cttctcagaa aacagttgag
     2581 gacaaggagc agaataatca gaaatcagct ttaaaaaccg atacatcaaa tagtacgaaa
     2641 aatatttttg ttaaaataaa gaatttaata aaaaaattga ttagtagttt atatcctaag
     2701 gaaaaggaaa taaatgtatc ttcagaaacc agtaataacc acgtggacct aatggagcag
     2761 aacgtaaaaa atattaaaat atataataaa gagttagata aaagagtaga agcactaaaa
     2821 aatattgatt tattacaaga aaagataagc caatataaag aaagggcagt agatcctaaa
     2881 gagtataata agaaaaaaga ttttgtgact gaagttatac agcgtaaaca acatgttgga
     2941 caaagtaaaa taaatagtag gtaagtaaca aatgttaata tgttccaaaa acgatcgttt
     3001 tggaaacata taacagactc gtaaaaatct ataaaaatct gcccaaaaaa tgtttcaaaa
     3061 cattgttctt attaaaatgt tccgctaata taacaaaaaa tcgttttagg aataagaaaa
     3121 tatgcttatc ggatacgcaa gagtctcaac gttagatcaa aaccctaatc atcaaattga
     3181 tgctcttaat gaggcaggat gtaaaaaaat atttacagaa aaaatatcag gtgcaagtaa
     3241 gcaacgtact caattgcagg cagctcttga ttatatgcgt gaaggcgata cgcttgtagt
     3301 ttggaaatta tctcgccttg caagatcgct tacgcaaata atcagtactt taaaaatcct
     3361 tgaggagaaa aaaatcagct tgcatgtaat tacccaaagt atagatactt caacagcaga
     3421 aggaagactt ttctttcata tgaatgcagc attcgatcag tttcagcgag agattattgt
     3481 agaaaatacc aaaaccggct taaaatctgc ccgtagaaat ggtagaattg gtgggagacc
     3541 taagtttatg agtgacgaaa aaatgcgtac ggcaaaagct atgctaaaag ataatgaaaa
     3601 ttatccgttt atttctgata ttataaaaac gctagatata ggtaggacaa ccttttatag
     3661 atactttcct ccaaagtcta tagagcaact tagaacccgc caataaaatc ctattggtaa
     3721 tttcgttcct ttctgaagaa gatccattaa acatacggga agattgtact aaaaaaccta
     3781 agccttgagg tgcagcattg ttgtaaagag ctgcaagtac tttaggagca tcagagcatt
     3841 gggaaatatg tattttcatg tttaggaatc ctttttatta gttaaattta tggtattgag
     3901 ttcagaaata gagagtccag taatacgtga gacctcagga attgagtaat tattagaaag
     3961 catattttta gctacctcca tagttctttt agcttcccca atctgcatac ctcgagcttc
     4021 cccaatctgc ataccttgga ccataccttt ctgtacgccc tcatcaatat atttatcagc
     4081 aatagttctc ataagctcct cttgatcttc tttttttaag tgcttagcta tgatactcgc
     4141 aagctctact tgtttatctt cagatacctt agcatcacta taccacaata accatttaat
     4201 gtaaatgtag ccattttcct tgtctatttc aatactactt tcaaacttct caagtaaact
     4261 ttgccaaaga tttaagatat cacgagcctt gatatgttta agcatatact ccatcatacc
     4321 aagatgtttc tttttctcta tttcatcatc actttgtttt tgcaaatcaa caagtaaata
     4381 ctcatcaccc atcatgtcct tagcagtctt gctatcttca aataactccc aaaagctcct
     4441 tggagcatta taaggcttat catttgcata tactacgata ggacatatta gagcctgacg
     4501 caaaacagcg ttattattac aagctatatt gcaaatattt taaaatattt agacgagtac
     4561 cataaaaata catggcaaga tgatttgact agcgtttatt tagcagctag ctataaaatg
     4621 ctgcaaatga atgaagaagc agagaaatta ttagacagat ttactctaaa taaacctata
     4681 tcaaaaacag actatcagta ttgttgcgtg gatcgatttt tccgtcaatg cgaagaattt
     4741 gcatagcaat tgatgaagca atctgcatac ctcgagcttc cccaatctgc ataccttgga
     4801 ccataccttt ctgtacgccc tcatcaatat atttatcagc aatagttctc ataagctcct
     4861 cttgatcttc tttttttaag tgcttagcta tgatactcgc aagctctact tgtttatctt
     4921 cagatacctt agcatcacta taccacaata accatttaat gtaaatgtag ccattttcct
     4981 tgtctatttc aatactactt tcaaacttct caagtaaact ttgccaaaga tttaagatat
     5041 cacgagcctt gatatgttta agcatatact ccatcatacc aagatgtttc tttttctcta
     5101 tttcatcatc actttgtttt tgcaaatcaa caagtaaata ctcatcaccc atcatgtcct
     5161 tagcagtctt gctatcttca aataactccc aaaagctcct tggagcatta taaggcttat
     5221 catttgcata tactacgata ggacatatta gaggaagctt gttatctttt tcttttgtaa
     5281 cactgctttt attgttattg gcgtccttat gcctttcgca taatagcagc atatattgcc
     5341 aaagtctaaa agcaatccag taatcgctac tagattgatg ttctataagt acgtatacgt
     5401 acgctttgtc attatggaca cttttttcag tagtactatc tttaatatta tcatttttta
     5461 aagaaacaga gtacacgaca tcgctgagtc tctttcttaa gtcttcagta acaaaacttt
     5521 ccttttctat tttgatacta tttaagttaa ttttattctt aaaggtcact ggcaaataat
     5581 gctctagaaa ctcacgagca gctacaggtt cgctcatgac ctttttaaat agctcatcat
     5641 gtcttgggcg ttcggaagtg ttgctactat tcgagtctac ctgctcatgg ttacgattgc
     5701 tttctttagt gtcagctatg cttaattcgc tagaatcttt cggtgtgttt ttgttgttca
     5761 tatacctctg tgcattaaga tttaatttta aaaatcctaa aatgttaaac cctacatatt
     5821 ctatgaaaag gcagatgttc ttgtattaag ctaactaaat gttgagcaga acatgagagt
     5881 atccagaatc caatgaagac aggtgtgaca tagttagtag gagatacgaa atgctgaccc
     5941 agatggaagt aattaatgat acaacaaagc cctagaaaga agaagagaaa aacattcata
     6001 ctatttaaaa tactaagaaa gacacaaaac gaataatgca atgttgtagc tatggttagt
     6061 ctaatagtaa atagggtgtc atagatagct ttgctgacca attgcagtat tgatttaaag
     6121 atattatatc gttttgtgtt ggcaatattt ctcttaaatg taatacgttc tttctcattt
     6181 ggaaaattaa taacattact cataatatac ctctaatatt aatacaaata ttaacttaat
     6241 tattaccttt tctcttcagt aatctagttt tattagcaat atttaaaaac tccttaataa
     6301 taggatggtc tttattatct ttattagtga caaattttag aaatccgatt agtaatttat
     6361 cgtcaatatc gacagcagaa aatttattaa agatattaaa tatctcttct tttcttttag
     6421 ttagaaggga agttaattgt acatttaatt ctgcttcaat cttttgaata cgttgttgct
     6481 ttacttttgc taagttagca atcttagtac tagttgagag tggttttgta gccatatgaa
     6541 atatttctat attatgatat agactagtat atctaaacta acaaaacaac actagcaaaa
     6601 agcacacttc ccaaattttt ataataaaac atgtcaaaaa aggttttact ttttacagat
     6661 atagcagggg gagtatgagg gggttcgcat aacccctctc atatagtaaa ctttagagcg
     6721 ttaaaaatta tgcataatag gcaagaattg ttgagatatt ttgaatatta tataacaaga
     6781 aagatgaatg atgttaagta taatgtttat actgtaatga tagttataaa atattacagc
     6841 aatataaata taatttaaag aaactgtcca agaaatattt tgaaattacg attgtaataa
     6901 tactttgtac gaagttttat tacaaaatac attattcatg agagccagtg tcagcaagta
     6961 aatagttttt atcagaaaac acaaaagacc cgtacggttt ttttaggact gcgctgtaac
     7021 tatccatcct taaccattcc tctatcttct tccatattaa agaatttgtt gcctccacac
     7081 cccccatcaa tatatactaa tacacagtaa agaagaagag agtagccgga tctgacccac
     7141 gcatataaca ctcaagtccc atctcatacc agcttggtac tctttgccat cctccaccta
     7201 acattgccgg ataagacgct tctacttctg aagtactatt taaagcctta ctcattaact
     7261 tacctccttt agttctacaa aacttccctt taatgattcg tttaaagcat taagtcttaa
     7321 atgaatgcct gctatctcct catattcctc aagtagaatt aaccctttgc acttagtctt
     7381 tagtgcctta tactcaagtt caattgaaaa aagattaact cctatgacgc tcagtatcac
     7441 gtcaatacag ctgatcgcct taatcacttt ctgcatactc ggtattccga gtttttcatg
     7501 cttcagtacc tcaaacatcc tatcaaaact ctcaattgct tccttaaaat cgtgattacg
     7561 gtaatggctg gtacctgcaa cctctaacca tgtgatgttt ctctcatctc ttactaatcc
     7621 gtacatatat attccctcct tattttgtgt ttaatttaat tatccttttt gcgattgtct
     7681 tttacactat tagcagcttc cgtgcttctt aattatttct taagtattaa tgattctgac
     7741 aataactttc tctttccttt agatttttta aaatcttaaa gactcacttt tagttttaaa
     7801 actccttgat ggctcttaaa tcgcttttgc ctttgctggt catacaatag ctctagctta
     7861 atgttcttat ggtttgctat taatccgatt ccagcattca ggcttgtcct ttcgttttga
     7921 gcattaactg atacaatgcc agtcccgata gtttgctggg cctcgtagta cgttaaagtg
     7981 aatgaattgc ttttgcccct actgcctatt cttctctcta ccgaaaaatg tgcagttggt
     8041 gttattgata ttgttgatgg agtactaccc tttagtttaa taggagcaaa tattactctg
     8101 ctgcctatct caccatttaa ggcttggtaa ctctttttag aacgctctat agacaggtta
     8161 cttgctattt gctctttgtg gcgacttact ttttcatatt cgtatctcaa tccaatgtga
     8221 ggtattagcg ttacttctcg cggcatctct actttgtagt taagatgagg ctcaaagctc
     8281 attaactttg atcttacttt tttacctttg ctatgtcctg ctgaacctat gatcgccacg
     8341 ttcatattgc ggcttactgg acgtgagcca tatagactaa atatatgagt atcatattta
     8401 gctcttgtac tcgtgccgct gctgcataaa tcacttgacc cgccttgatc ttgatattta
     8461 acacggctat taaacctgct aaaactagca ccgagtagca aatcattctc aaggttgata
     8521 tctgctccaa tagtgcctcc agctattttg ctactatagc cattcgagtc tgctttagat
     8581 ttttgtttgt taatgccaag cgtgccacta ctccatatac tatagccgat ggttttttca
     8641 gatgcatcat cggcgtcatc atcaccgcta gctacagcag cagttactat atctctagca
     8701 aagatacggt ctctgatctg tttactagat attttgctca tcttccgtgc ttggttttgc
     8761 attacggatg ttgcctcggc tttcttacgt aactcttcct gactcataga ggtagcttgt
     8821 tcttgtggct cagtgcttgc agtactacct aagttagtag atatgtcttt agatacacta
     8881 tctttttcgt ccacctcatc tagtatctca aagacgcttg ctgtggtact ttcttcctgt
     8941 gatgcttcct ctgattcgtt gctgtctgct gcaatattgg cttcattaat gctagcgtta
     9001 cttgcaacag ctgaagattg ctccgatact tgaaactctc ttatgatgtc aagctccgct
     9061 ttcttctctt cagtcgcagg gggcgaaaat ggtatcccgt cctcgtcgct actatcctca
     9121 gaatattcac taccactact tgtacataca gtgtcatatc catcagtatc tgcaatacta
     9181 tcctcaccac tccaatcatc aacgcctcca caactagaac cgctataacc gctatcttct
     9241 attgcctcat ctagcatctt cattactcgt tcttcctcgc ttaatgaagc atcttctgaa
     9301 atatgtaagt cttctaaact actaagcact gatagcggtg gtgctgtcgg ctcaatttgc
     9361 ttctgcatac taaattcagt ttcaaatgct tcacctattt cagtatcagg atcttgccaa
     9421 ggttcagagt actccgtaac gctacttgcc atactaatag tatctatagg cttgctgtat
     9481 ataggctctt ctgctcctcc gcctagtgtc cctgctaaat tattgatacc ttgatttttt
     9541 agctgctctc ttaagagtgc tatctcagat cttaaatctg atactatgtt gtgatgtagt
     9601 gctggatcta taccattaac tacaccttcc ctattctcgc ctctttcctt tgctttttgc
     9661 tctatcctag cttttactgc ttcgacttca gctcttcttc tttctcttgc tatctccgct
     9721 ttcttccgtg cttgctcctc agatgttaat ttaggtaatt gctttactac tttctttggc
     9781 ttagcgccgt tcttctctcc agctatacca tttcttcttc tctcaagtgt attgtgtagc
     9841 tgactcatta gatcctcgcc atcgctagat ataactttag gctttggctc tctcttgacc
     9901 ttttttaata tgtttttgcc gaaattcctt atcgactcaa acaatgctga cctattgtct
     9961 gcttttcctg ctatatttgc accactgatg aaatcaggca tagcaggtag cacaggcgga
    10021 attggaggag ctggtggtag tgactgtggc atgattggtg gtaaaggcac gttagtgcta
    10081 attcctgcag agcccttacc aaataaaaaa ctttgatttg cttctataac tggttgcgcc
    10141 ttgacttcta acgtagttgc aataaatcca agtgatagca acatcactga tgctactcga
    10201 gatatcttgc ggaactttga tttaaaatta agattaaggt tgcctttata cttctttcta
    10261 tagtagtgag cattgctact actaggtaaa aagtagtcgg gggttaggta tttgggggta
    10321 acgtttaaat ttgcctgatg ggataaactc tctgctgata tgcccttagc ttcaaaaata
    10381 ttacctttgt tgccaaagcc atgaatatat gatagtttca taatataaac ctttgtttcg
    10441 gggttttagt ttcaataggt gggtactcaa ccagcaaaag tagtgtatcc acttagacta
    10501 gattaataaa aaaaacaaag taagtcaaat aaattccgaa tatttctcgg aatttatttt
    10561 ttaaagtata tttatgtcga taactataag cattcagaaa tttttaaaag ttaaatttta
    10621 tgaacataaa attttacgaa aagattttat taatgaaagt gggatcaaac gttctagtat
    10681 tagtgaatta ttaaatggca tacctgtaag cccttcttta gttacaattc ttaaaattgc
    10741 taattacttt aattgctcaa ttgatgaaat tttaggaagg gtggattata tacaggatga
    10801 taatactaaa tatatcaaga tcagtccctt acaaatgtct aacaacacta aagattttat
    10861 tgtggcgatg ctaaatgaaa ataatatttc atctcaccaa ttagctgctt catgtaatat
    10921 cggaacagat actataaact attttataaa aaaacatgat gctaagaaaa atcttagtat
    10981 tagaacaata tatgccgtcg caaacttttt taatatttca atagataaaa tagttggtag
    11041 aatatagtag ctagacgtct agctagctag atatctagct agtgcttttt caagtacttc
    11101 taccatataa cattgttctt ttattgctat cttattcaaa ttattataaa agtccattgt
    11161 tacctttaga tttaattgaa catttctctc gcttcttctt ttggttctta taagactagt
    11221 ttctagttct tcattccgcc ttggttttct tatttcccct aatgtttcta gatttagtgg
    11281 tttttttttc attactcttg atttctcctt ctataaaatt acataattcg ttaatctcac
    11341 tgatagcagc tttattatac atctctaatg cagttcttcc atctaacatc gcaatagcat
    11401 atataatcct ttgtcttatg ggcgatggtg caatctgacc attttttgat agtagtaaaa
    11461 ttgtctgctc tgttatttga gtccctgaca cagaactatt caaaataaag gtaaaattct
    11521 ttttatgttc ttctattata gttaaagttt cacctatagc ttcaatatcc aaaggcgaag
    11581 gtctacatgg tattaatatg taatctgcta caagtatagc ttgttcaatt atttttaaat
    11641 gcgatggtgg tgtatctata ataacatagc tgtcttttgt atttattttt attagagcat
    11701 cttttaattg attatattta atatctacaa gagatatatc ttcattttct cttttttgcc
    11761 accattgttt taaactttgt tgtggatcta aatctattag atataccttc tttttctttt
    11821 ttgctaaatt gcttgctata agggcagtaa ttgttgtttt tcctactcct cctttattag
    11881 cagcaatagt aattacagac atttttataa ctaagtaata ttacatagat atctagctat
    11941 ctgtatagct agatatctat ataatattac ttctctgttt tttttcaaga ttaatattaa
    12001 aatttttaaa agaatatgtt aatgcttcta tccgaatttt attcgaattc ttttgttgac
    12061 actgtaggat tatttttcta gtctttagat attgatgttt aggcaggtac actacttttg
    12121 ccggttgagt acccacctat tgaaactaaa accccgaaac aaaggtttat gaagtgaata
    12181 taccacacaa cgaacaaaaa cacaacaata atcaaacatc cctacaacac ataggcaata
    12241 tagcctatca ccaaggaaaa agtgtaacta attatgatgt tattaattcc ttaactcttc
    12301 ctaataagca ttatactttg ctttatcctg atttagtgca agaattattt aatttttcgt
    12361 tgcataatag caacagt
//
