LOCUS       NC_009516              13956 bp    DNA     circular BCT 26-JUL-2008
DEFINITION  Psychrobacter sp. PRwf-1 plasmid pRWF101, complete sequence.
ACCESSION   NC_009516
VERSION     NC_009516.1  GI:148641524
DBLINK      Project:15759
KEYWORDS    .
SOURCE      Psychrobacter sp. PRwf-1
  ORGANISM  Psychrobacter sp. PRwf-1
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Moraxellaceae; Psychrobacter.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina
            del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,
            Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F.,
            Land,M., Hauser,L., Kyrpides,N., Kim,E., Tiedje,J. and
            Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of plasmid pRWF101 of Psychrobacter sp. PEwf-1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2007) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 13956)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina
            del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,
            Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F.,
            Land,M., Hauser,L., Kyrpides,N., Kim,E., Tiedjej,J. and
            Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2007) US DOE Joint Genome Institute, 2800
            Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CP000714.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4001101
            Source DNA and bacteria available from James Tiedje
            (tiedjej@pilot.msu.edu)
            Contacts: James Tiedje (tiedjej@pilot.msu.edu)
                      Paul Richardson (microbes@cuba.jgi-psf.org)
            Quality assurance done by JGI-Stanford
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LLNL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Psychrobacter sp. PRwf-1"
                     /mol_type="genomic DNA"
                     /strain="PRwf-1"
                     /db_xref="taxon:349106"
                     /plasmid="pRWF101"
     gene            219..590
                     /locus_tag="PsycPRwf_2391"
                     /db_xref="GeneID:5204460"
     CDS             219..590
                     /locus_tag="PsycPRwf_2391"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_001274369.1"
                     /db_xref="GI:148641525"
                     /db_xref="GeneID:5204460"
                     /translation="MSKRAENMSRVNDLRSKVTSAMISLLDELEEGTGGDYYGFTEWD
                     IKNHQELKGQLNSYRAQKIAQFLGRTISKQKLLKYAKPKGYEYSLTNKDISNWLESNK
                     DALLKYSSFNIGVMTNGHRYE"
     gene            complement(634..1863)
                     /locus_tag="PsycPRwf_2392"
                     /db_xref="GeneID:5204468"
     CDS             complement(634..1863)
                     /locus_tag="PsycPRwf_2392"
                     /note="PFAM: plasmid replicase; Primase, C-terminal 1"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replicase"
                     /protein_id="YP_001274370.1"
                     /db_xref="GI:148641526"
                     /db_xref="InterPro:IPR004322"
                     /db_xref="InterPro:IPR014820"
                     /db_xref="GeneID:5204468"
                     /translation="MECLKQVSPREWQKVPQLRQFYDDLANKPYCTNEKGSIFVRSKK
                     QAIKHRFIQPNHPCVIKWLVFDIDHPDALFTFIDTGLPKPQVIIKNPKNGHAHYAYRL
                     TTPVGIGGNSSTKAESYLRAVKDALCEALGADTSYSGNLIKNPCYIKDNAPTPKKTHW
                     LNNNEDEGDRDEHETYLTGAQPSYTLAELADYLDLEPLYPQTKQEPANDSGYGRNCSL
                     FEELRHLAYQCKTKSFNAIVRYLKPIAEKMNQRFDVPLNPNEVKHIIRSIARYCARMD
                     FTASHKAFSKLQATRGAKGGRAKGRSYDEKRQQAKELYEQGLNKSEIARKLDVSRRSV
                     INWLSNLECANSHNQIIAALGAGLQFAMPRSLVRTIKSSSCPLAAPYSVASSLPSVVI
                     VRNFTRLAVNSVFRPPP"
     gene            2447..5251
                     /locus_tag="PsycPRwf_2393"
                     /db_xref="GeneID:5204471"
     CDS             2447..5251
                     /locus_tag="PsycPRwf_2393"
                     /codon_start=1
                     /transl_table=11
                     /product="Type II restriction enzyme methylase
                     subunits-like protein"
                     /protein_id="YP_001274371.1"
                     /db_xref="GI:148641527"
                     /db_xref="REBASE:PspPRORF2393P"
                     /db_xref="GeneID:5204471"
                     /translation="MSIDYKHVRQQLQQIVHDYKDSEGYERGQSQNFWTQVFNAYGVS
                     GQTQTKAFEHRLKDKSNQKYVDAFIPKLVIIEQKSRGVDLNKAYTQVSEYYDRINAKD
                     KPRYIILCNFDEIWLYDINNPLDIKKHQCPLSDLPNNAEWFEFLSPESQQSNEIIEEN
                     PINRQATEKLAKLHQAFIEDGVDPDELALFLTRLIFCFFADDTAIFGKKHVLHNLLKN
                     HAATDGSNLQQILTTLFDTLNTEHRSSRLPEHYAQFAYINGGLFEETINIPYFDEKLY
                     NLVMECDALDWTEISPAIFGSMFQSVLDASGGDSTEDKRREFGAHYTSEKNILKVINS
                     LFLQELRDEFSKCTNNTPRAVQLYEKLPTLKFFDPACGCGNFLIIAYRELRLLENQLI
                     AKIFGDQKGLLDISSMCNVTVDQFYGIEIEPHAVHIARVAMWITDHQLNMTTAERFGT
                     TRPTTPIVYSPHIIEGNALQIDWETVLPANDCSYVMGNPPFIGKSNQSSEQKSDIKLV
                     ASHIKNHKSLDYVAGWYIKSMHYMQSVNNANHYIDTAFVSTNSIVQGEQVDILWRYLI
                     DDCKGHINFAHHTFKWSNEGKGIAAVHCIIVGFSLVEKKEKTIFEYSDISSEPSPKKA
                     RTINAYLTDAPIVFFSRRSKQVSNESSMVSGNKATDGGNLILSDSEYIDLINSEPLAK
                     KYIKRFMMGYEFLNNIKRWCLWFDNVDPIQLSKDLEKMPLIKKRIHNVKELRLNSTKK
                     STVKKAETPHLFDERRHTNKPYVAIPVVSSENRRFIPIGFIDGNTVAGNKLFVIVDGN
                     TYQFGTLSSSMHNAFMRLTAGRMKSDYSYSSTIVYNNFPYPFMADDHSDKAQKARESI
                     AKASQQVLDARKHYQDGSENAPTLAQLYNTYLIDPYPLLTKAHKALDKAVDSAYGYRG
                     KGDDASRVEFLIKKIAELKN"
     gene            5853..6419
                     /locus_tag="PsycPRwf_2394"
                     /db_xref="GeneID:5204469"
     CDS             5853..6419
                     /locus_tag="PsycPRwf_2394"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_001274372.1"
                     /db_xref="GI:148641528"
                     /db_xref="GeneID:5204469"
                     /translation="MNISVSEASKRWGVSRTTIYKKIDDGELSRNSDKKIDTTEMLRV
                     FGNPPSTERTEQNKNNVHWTPVNSEAEQENVQLKHQLEMEKLKNVHLQQQVDHHKQLV
                     ENYQKQIDQLSKSLDKANASIQDFAQTKLLEMKGSLSGSSISQDENNSKDNQGDSEIV
                     SSDSNSKPATNQEAINSKGKKRWWSLSL"
     gene            6548..6895
                     /locus_tag="PsycPRwf_2395"
                     /db_xref="GeneID:5204466"
     CDS             6548..6895
                     /locus_tag="PsycPRwf_2395"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_001274373.1"
                     /db_xref="GI:148641529"
                     /db_xref="GeneID:5204466"
                     /translation="MWTVITTDLFNDWLYQQDQSTQEKVLAALVVLQQQGPSLGRPLV
                     DTVYDSKFTNMKELRVQHRGKPLRAFFAFDPKRQAIVLCIGDKGNNKRFYKEMLATAD
                     QQYEYHLKTLGDL"
     gene            6897..7199
                     /locus_tag="PsycPRwf_2396"
                     /db_xref="GeneID:5204465"
     CDS             6897..7199
                     /locus_tag="PsycPRwf_2396"
                     /note="PFAM: helix-turn-helix domain protein"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="YP_001274374.1"
                     /db_xref="GI:148641530"
                     /db_xref="InterPro:IPR001387"
                     /db_xref="GeneID:5204465"
                     /translation="MTKTLQELLAERSLDSQARIQQLAEQLFLENQLYRIREELEISQ
                     KELAQTLGIKQPSLSAIENRGNDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFTV"
     gene            complement(7214..7846)
                     /locus_tag="PsycPRwf_2397"
                     /db_xref="GeneID:5204470"
     CDS             complement(7214..7846)
                     /locus_tag="PsycPRwf_2397"
                     /note="PFAM: Resolvase, N-terminal domain"
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase domain-containing protein"
                     /protein_id="YP_001274375.1"
                     /db_xref="GI:148641531"
                     /db_xref="InterPro:IPR006118"
                     /db_xref="InterPro:IPR006119"
                     /db_xref="GeneID:5204470"
                     /translation="MFIRAYLRASTKEQDANRAKDELIAFAREHGHKIAAFYTENESG
                     AMLERPQLMQLIDDASEGDVILVEQIDRLARLNQTDWDTLKRKLSAKRLSVVSKELPT
                     SYMALQQGNGSEFMDSVLRAINDMLLDMLAAIARKDYEDRRNRQMQGIARAKAQGKYK
                     GRGKDMEKRKIIASLLKSGHSYSDIQQTVKCSRQLIAEVSKASKPISSSI"
     gene            complement(7991..8200)
                     /locus_tag="PsycPRwf_2398"
                     /pseudo
                     /db_xref="GeneID:5204462"
     gene            8779..9417
                     /locus_tag="PsycPRwf_2399"
                     /db_xref="GeneID:5204457"
     CDS             8779..9417
                     /locus_tag="PsycPRwf_2399"
                     /note="PFAM: Sel1 domain protein repeat-containing
                     protein"
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 domain-containing protein"
                     /protein_id="YP_001274376.1"
                     /db_xref="GI:148641532"
                     /db_xref="InterPro:IPR006597"
                     /db_xref="GeneID:5204457"
                     /translation="MKKRLAVFLLTCIMLTGCTKVIDTQRESTQNNVPTVKSDVEKDF
                     ENLTLQANLGDASSQAELGLIYYYGRGVPQDYSKAFYWLQQAANHGHKEMQGMLALMY
                     EKGQGVDQNDAKAFELYQNLANDGIDLYQSKIGDMYLKGKGTTQSDTKAFEWTRKAAL
                     QDLPMAQSGLAVFYEKGINIQQDKNKALEWYQKSCSNGYPFACAEYRRLYQK"
     sig_peptide     8779..8838
                     /locus_tag="PsycPRwf_2399"
                     /product="hypothetical protein"
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probabilty 0.945) with cleavage site probability 0.345 at
                     residue 20"
     gene            complement(9499..9951)
                     /locus_tag="PsycPRwf_2400"
                     /db_xref="GeneID:5204458"
     CDS             complement(9499..9951)
                     /locus_tag="PsycPRwf_2400"
                     /note="PFAM: protein of unknown function DUF1200"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_001274377.1"
                     /db_xref="GI:148641533"
                     /db_xref="InterPro:IPR009589"
                     /db_xref="GeneID:5204458"
                     /translation="MINTIFTSKIDFWLAFLILGSSLLLVLVPVWEWMYNNSSVKRII
                     FISLFTIPTALLLLVLFFNIKYTLTADTLLVKNGFSTQSISLEDITHITPTSSTLSAA
                     ALSLDRIEIRYEGGSIVISPKDKDRFYHAIQERVPALKTDDNNGLIKR"
     sig_peptide     complement(9499..9597)
                     /locus_tag="PsycPRwf_2400"
                     /product="hypothetical protein"
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probabilty 0.693) with cleavage site probability 0.560 at
                     residue 33"
     gene            10120..10461
                     /locus_tag="PsycPRwf_2401"
                     /db_xref="GeneID:5204464"
     CDS             10120..10461
                     /locus_tag="PsycPRwf_2401"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_001274378.1"
                     /db_xref="GI:148641534"
                     /db_xref="GeneID:5204464"
                     /translation="MVPYGYTAFIIDVFVRSIVGWKVSNRMNIDMVMAALNQAIADRN
                     NPKDVIHRSDRGVQYLSIRYTDKMADSGVITFVGTRVIHMIMHSTIGYVSPFEFEKQY
                     YDRLNESGKVA"
     gene            complement(10780..11226)
                     /locus_tag="PsycPRwf_2402"
                     /db_xref="GeneID:5204463"
     CDS             complement(10780..11226)
                     /locus_tag="PsycPRwf_2402"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_001274379.1"
                     /db_xref="GI:148641535"
                     /db_xref="GeneID:5204463"
                     /translation="MRSLLLTTMCLTGSLALVACNNHRADTTHLSKQPTIEVPVASAA
                     FTAFGNTAKEKIWRIVIEGNELSIEANFLKPTTATVIVVGSGSVNTEGVEYASTINGQ
                     PIVVNIKNNLCIDDNGYQNELTAILTYAGEVYQGCAVAGAIEIAPT"
     sig_peptide     complement(10780..10839)
                     /locus_tag="PsycPRwf_2402"
                     /product="hypothetical protein"
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probabilty 0.998) with cleavage site probability 0.987 at
                     residue 20"
     gene            11570..12322
                     /locus_tag="PsycPRwf_2403"
                     /db_xref="GeneID:5204461"
     CDS             11570..12322
                     /locus_tag="PsycPRwf_2403"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_001274380.1"
                     /db_xref="GI:148641536"
                     /db_xref="GeneID:5204461"
                     /translation="MKSKKILALAFVMSCIAALAGCNTTPDNSSQYTNAVETSHKNSH
                     SNIANPQVLDIKGSITGYDTKEYIFHAQANQVLRVVRQNTSDDVVLKLLYLGNNNKTS
                     ELSGDFQVLPYTGDYKLIVSQTRNDARKHANTAKNFNLKAFITDQQQPSNQQNYVVNY
                     KCDSNENLSVTYHFNNQKTSATVTYDNISQQLNFDDQYSKKNNPVFSNNNYLLSVGTI
                     NDGVFGDSMVYSLLDLKKSKGGYALLQNCQKL"
     sig_peptide     11570..11632
                     /locus_tag="PsycPRwf_2403"
                     /product="hypothetical protein"
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probabilty 1.000) with cleavage site probability 0.700 at
                     residue 21"
     gene            complement(12399..13445)
                     /locus_tag="PsycPRwf_2404"
                     /db_xref="GeneID:5204459"
     CDS             complement(12399..13445)
                     /locus_tag="PsycPRwf_2404"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_001274381.1"
                     /db_xref="GI:148641537"
                     /db_xref="GeneID:5204459"
                     /translation="MPRKHAKVLYGDPVLTEHLIDTTVHKSKYKSGYLSFSERADEIS
                     EDDKKRIMREFEQVIFCGLEPDQYDILWVEHADKDIDEENPVGRLELNFVIPCQELRS
                     GKSFQPYYEPADQRRVNAWKNIVNAEIKTIHEQPLSDPNDPKRQRLVNPYNSHAPRPS
                     PFNINSYSKKQSDEDDEIIQHPKSRRDLEEALKRRLLLESQRGIVTNRQSLLTTLRRW
                     GLNINRSNSETSVSVKHDDLKDKNGRVMSVRLTGGMFAKDYKGVEFEPYIQEVNSNAY
                     YSEPNKVHRSQLDKKNLEEGIKIKAQYHQERYKEATLPEPLAIQKAKVTNSVEEAPSP
                     KAEPSKRYMPSFRR"
     gene            complement(13525..13890)
                     /locus_tag="PsycPRwf_2405"
                     /db_xref="GeneID:5204467"
     CDS             complement(13525..13890)
                     /locus_tag="PsycPRwf_2405"
                     /note="PFAM: mobilisation protein"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilisation protein"
                     /protein_id="YP_001274382.1"
                     /db_xref="GI:148641538"
                     /db_xref="InterPro:IPR008687"
                     /db_xref="GeneID:5204467"
                     /translation="MSNNSPINLDKRAKRNREISIRVNDYELLELKKRNRGSTVASWL
                     RDLALGVTPVKPVDPGLVRQLGRIGSNLNQLTRHVNTENQVDAQVLHEIKAIREQIHL
                     LIESSIEASKKSVRDNRDS"
ORIGIN      
        1 ggatattttt agcgtagcat aaaatagccc cctattgtga ctgctggctt aataaaagac
       61 ttacaattgc ctgtacctac tataatgttc tatgtctgta cctaataata catcactgtc
      121 tatacctaat tacagcctaa tgtctgtacc cataatttat taatgtctat acctatgcta
      181 aaataactgt gtatacctaa aaatataagg cagttattat gagtaaaaga gctgagaata
      241 tgagtagagt taatgacctt cgctcaaaag taacgagtgc tatgatctct ttattagatg
      301 aattagaaga gggtacaggt ggtgattatt atggttttac tgagtgggat attaaaaatc
      361 atcaagaact aaaaggacaa ttaaattctt atagagcaca gaaaattgcc cagttcttag
      421 gcaggactat tagtaagcaa aaattattaa agtacgctaa gcctaaagga tatgagtatt
      481 ccctaaccaa taaagatatt agtaactggt tagagagtaa taaagatgcg ttattgaaat
      541 attcatcttt taatatagga gtaatgacaa atggccacag gtatgagtaa ggttgcaact
      601 tagcagcact ttggccaccc agccctttac ttcctaaggc ggtggccgga aaacagaatt
      661 cacagccaag cgagtaaaat tccgcacaat aacaacagaa ggaagtgaag aggctacact
      721 atagggagcc gctagaggac aagaagacga tttaatagtc cgaaccagtg accgcggcat
      781 cgcaaattgc aaaccagcac ccaaagcggc tattatctga ttatggctat ttgcacactc
      841 taggttgctc agccaattga tgactgaacg ccttgataca tctagctttc gtgctatttc
      901 actcttattt aagccttgtt cgtataactc tttagcttgc tgtcgtttct catcatagct
      961 cctaccttta gctcggccac ctttagcacc tctagtagct tgaagcttag agaacgcctt
     1021 atgtgatgct gtgaagtcca ttctggcaca atacctagcg atactacgta tgatgtgctt
     1081 aacctcattt ggattaagtg gtacatcaaa tctctggttc atcttttctg ctattggttt
     1141 aagatatctg actattgcat taaaactttt agttttacac tgataggcta agtgtcgtaa
     1201 ctcctcaaat aacgagcagt tacggccata acctgaatca tttgcaggct cttgcttcgt
     1261 ctgagggtag agtggctcta aatctagata atctgcaagc tctgcaagcg tataagaggg
     1321 ctgcgcccct gttagatacg tctcatgctc gtcacgatcg ccttcgtcct cattgttgtt
     1381 aagccagtga gtcttttttg gggtgggtgc attgtctttt atgtaacaag ggttcttgat
     1441 gaggttgcct gaatagctcg tatctgctcc taatgcttca catagggcat cttttacagc
     1501 tctaagatag ctttcggctt tagtacttga gttacctcca atacctacag gtgtggttag
     1561 cctatacgca tagtgggcgt ggccgttttt gggatttttg attataactt ggggtttggg
     1621 taggcctgta tctataaagg taaataaggc atctggatgg tcaatatcaa atactaacca
     1681 cttaatcaca caagggtgat tgggctgaat aaatcggtgt ttaatcgctt gttttttgct
     1741 acgaacgaaa atagagcctt tctcgttggt gcaataaggc ttattggcga ggtcgtcata
     1801 aaactgacgt aattgaggta ctttctgcca ttctcttggc gagacttgtt tgagacattc
     1861 cattacattc tacgctattg ttttagagtg atagcgtgat ataatggatc ttgcgatgga
     1921 accactatat ctgctaccac agattagtat tctagaaacc ctcatagcct gcaagctatg
     1981 ggggtttcgt ctttttagct agtttactct tttgttacac tctctgcaaa cctttatatt
     2041 tcaaggctta aatagaatat tatcctattt aaataagtca gcctggccat gcttacgtac
     2101 gtaatcacgc ataaaggctc tgacagcctg tgatgccgtt gtatcttggt tctgacaagc
     2161 gtgtttaaat gcttttagta gatcttcatc aactttagca gtgaacattt ttgtttttgg
     2221 ctcagtcata cttagcactc catagataat atagagtaag tatataccaa ataaacacca
     2281 gttaataccg gtattaactg gctttactca tacacaagtg ccgccctcca caaaacccat
     2341 agcaagcgat ttttttgggt cgataggcat tcctaaactg tgttagcgtg tggtactgta
     2401 tccggcttga atgctctcat aagaacatac aggttgaaga ataaaaatga gtatagatta
     2461 caagcacgtc agacaacaat tacaacaaat cgttcacgac tataaagact ctgagggcta
     2521 tgagcgtggc caaagccaaa acttttggac tcaagtgttt aatgcttatg gcgtgtctgg
     2581 ccaaactcaa actaaagcat ttgaacatcg tcttaaagac aaatctaatc aaaaatacgt
     2641 tgatgctttc atccccaaat tggtcataat tgagcaaaaa agtcgtggtg tagatttaaa
     2701 taaagcctat acacaggtgt ctgagtatta cgatcgtatt aacgctaaag acaagcctag
     2761 atacatcatc ttatgcaact tcgatgaaat ttggctgtat gacatcaaca acccattaga
     2821 tattaaaaag catcaatgtc cactctctga tctgccaaac aacgctgaat ggttcgagtt
     2881 cttatcgcct gaaagccaac aatctaatga gattatcgaa gaaaacccca tcaaccgaca
     2941 agctactgaa aagctagcta aactgcacca ggctttcatt gaggatggtg tagatcctga
     3001 tgaattagcc ttatttttaa cacgcctaat cttctgtttc tttgctgacg acaccgctat
     3061 ttttggtaaa aaacacgtac tgcacaattt gttaaaaaac catgcagcca ccgatggtag
     3121 taacttacag cagatactaa ccactttatt tgacacatta aacactgagc atcgttcaag
     3181 cagattgcct gagcattatg ctcaattcgc ctatatcaat ggcggtcttt ttgaagaaac
     3241 tatcaacatc ccttatttcg atgaaaagct atataaccta gttatggagt gtgatgcact
     3301 cgattggact gagattagcc ctgcaatctt cggttcgatg ttccagagtg tattggatgc
     3361 tagtggggga gatagcactg aggataaacg gcgtgagttt ggtgctcact acaccagtga
     3421 gaagaatatt ctaaaagtca tcaactcatt gtttttacaa gagttacgtg atgagttttc
     3481 taagtgtact aacaacacac caagagccgt acagctatat gaaaaactgc ctacactaaa
     3541 gttctttgac cctgcttgtg gttgcggtaa ctttttaatc attgcctatc gtgaattacg
     3601 tctattagaa aaccagttga ttgccaagat atttggtgat caaaagggat tacttgatat
     3661 tagcagtatg tgtaatgtga ccgtagatca gttttacggc attgagattg aacctcatgc
     3721 cgttcatatc gctcgtgttg ctatgtggat cactgaccac cagttaaaca tgaccactgc
     3781 ggagcgtttt ggcacaacca gaccgaccac accgattgtt tatagccctc atattattga
     3841 aggtaatgcc ttacaaatag attgggaaac agtcttacct gccaatgatt gtagctatgt
     3901 aatgggaaat cctccattta tcgggaaatc caatcaaagt tctgaacaaa agtcagatat
     3961 aaaattagta gctagccata ttaaaaatca caagtcttta gactatgtag caggttggta
     4021 tataaaatcc atgcattata tgcaatcagt taataatgca aatcattata tagatacagc
     4081 ttttgtatca acaaactcga tagttcaagg tgagcaagtt gacatcctat ggagatatct
     4141 aattgatgat tgcaaaggcc atataaactt cgcacatcat acctttaaat ggagcaatga
     4201 gggcaaaggg atagctgcgg ttcattgcat tattgttggc ttttctttag tagaaaagaa
     4261 agagaaaacc atcttcgaat actctgacat ttcgtcagaa ccaagcccca aaaaagctag
     4321 aaccatcaat gcatatttaa ctgacgctcc aatagttttc tttagtagaa gaagtaaaca
     4381 agtttccaac gaaagtagta tggttagtgg caacaaggca acagatggag gtaacttaat
     4441 tctgtcagac tcagagtata tagatttaat taattcagag ccattagcta agaaatacat
     4501 taaacgtttt atgatgggct atgaatttct taacaatatt aagcgatggt gtctgtggtt
     4561 tgataatgtt gacccaatac aattaagtaa agatcttgaa aaaatgcctc ttattaaaaa
     4621 gcgcattcat aatgtcaaag aactgcgttt gaacagcact aaaaagtcta ctgtcaaaaa
     4681 ggcagaaaca cctcatttgt tcgatgaaag acggcatact aataaacctt acgttgcaat
     4741 acccgtcgta tcatcagaga acagaagatt tataccgatt ggctttattg atggtaacac
     4801 cgtagcaggt aacaagttat ttgtaattgt agatggtaat acctatcagt tcggtactct
     4861 gtctagcagt atgcataacg catttatgag actaacagcg ggtagaatga aaagtgacta
     4921 tagctattca agcaccattg tttataacaa ctttccttac ccatttatgg ctgatgatca
     4981 tagtgataaa gcacaaaaag cgagagaaag catagctaag gcttcacaac aggttttaga
     5041 tgctcgtaaa cactatcaag acggtagtga gaacgcacca accctggctc agttatacaa
     5101 tacctatcta attgatccat atccactact aaccaaggct cataaagcgt tagataaggc
     5161 cgttgatagt gcttatggtt atcgtggcaa aggtgatgat gcgagtcgag tcgagttttt
     5221 gattaagaag attgctgagt taaaaaatta acaaaaaaaa gcccctttcc aggggctttt
     5281 ctattaactg gcacttaaaa gacaatgaac gaaagtcatt taacgtaacg tctgttatgg
     5341 gaataatgat attttccgtt aagccgcata attaaaactt cccaagttta actgtacgga
     5401 tttgacagca agggtcattt tttaaaatgc ggtttttttt gtatgtcaat ttcttataaa
     5461 actaatgcct aaccctatta ataaaatagt aaggatagct atgaatagca tcctgatctg
     5521 aaccagttgt attttttctg caatctttat agcatcttta gtactattaa attttttaag
     5581 tttggtcgtt tgttttttct ggcgcatatt tttatccctt tataatttta cttcatatct
     5641 tatgggatat gagctattcc atttttagtt taggctaaac ctaaatttat gcccctttat
     5701 tagaggctat aacaaagtat aaggatgtta attgagcctt tcttaggtag ttttagtata
     5761 tggctttcgg tgaaattgga caaccatcta gaacaactat gatactgtac taggtataca
     5821 tcagaggaca tcattgaaca aagagaggct atgtgaacat atcagtatca gaagcatcaa
     5881 aaagatgggg tgtatcaagg accacaatct acaaaaagat agatgatggc gagctttcaa
     5941 gaaattcaga caagaaaatc gatactactg aaatgttacg ggtgtttggg aacccaccaa
     6001 gcactgaacg tactgaacaa aataaaaata atgttcactg gacgccagtg aacagtgaag
     6061 ctgaacagga aaacgttcag ctaaaacatc aactggagat ggagaaacta aaaaatgtcc
     6121 acctgcagca gcaggtggat catcacaagc agttggttga aaattatcaa aagcaaattg
     6181 accagttaag taagagcttg gacaaagcga atgctagtat tcaggacttt gcacaaacta
     6241 agctattaga gatgaaaggt agcttatcag gatcgagtat ctctcaagac gaaaacaact
     6301 ccaaggacaa tcagggagac agtgaaatag taagttctga ttcaaattca aaacctgcta
     6361 ctaatcaaga agctatcaac agtaagggca agaaaagatg gtggagcttg tctttgtaaa
     6421 tgctctcatg gatagttagg ttttaacgag caaaaccttt acttgttaaa gagtagctaa
     6481 gtatcacagt tgtcatgagc atttattagg agtagtatat agactataat ctatattatg
     6541 gttttatatg tggactgtca tcacaaccga tctattcaat gattggcttt atcagcaaga
     6601 tcaatcgaca caagagaagg tgcttgccgc tttagtggtt ttacagcagc aaggcccaag
     6661 cctagggcgc cctttagtgg acactgtata tgactcaaaa tttaccaaca tgaaagagct
     6721 tcgggttcag catcgaggta aaccactgag agcctttttc gcatttgatc cgaaaaggca
     6781 agcgattgta ctttgtatcg gtgataaggg taataacaag cgcttttata aagaaatgct
     6841 agccacagcc gatcagcagt atgagtatca ccttaaaacc ttgggggatt tataacatga
     6901 ctaagacatt acaagagcta ttagcagagc gctctttaga cagccaggcg cgtattcagc
     6961 aactggccga gcagctattt ttggaaaacc aactctaccg catccgagag gagcttgaaa
     7021 tctctcaaaa agaacttgct caaactttgg gtattaagca gccttcacta tctgctatag
     7081 aaaaccgtgg caatgacctt aaaatctcaa ccatgaaaaa atatgttgaa gccatgggtg
     7141 gtaagcttcg tattgatgtg gagctgccaa cggggaagca tattggcttt actgtataaa
     7201 tatattcgtc aggttatata gaggaagata ttggttttga tgcctttgaa acctcagcaa
     7261 tcaattgtct tgaacacttc acagtctgtt gaatatcaga gtagctgtgg cctgatttta
     7321 gaagactggc aataatttta cgcttttcca tgtccttccc ccgcccttta tatttacctt
     7381 gagctttagc gcgtgcaata ccttgcattt gacggttacg tctatcctca taatcctttc
     7441 tagctatagc cgctaacata tccagcagca tgtcatttat ggcgcgtagt acactgtcca
     7501 taaactccga gccattacct tgttgcaatg ccatatatga cgttggaagc tctttagaca
     7561 ctacagaaag acgtttggct gatagttttc tctttaaggt atcccaatca gtttgattta
     7621 aacgtgccaa acggtctatc tgttcaacta agatcacatc gccctcagaa gcatcatcaa
     7681 tgagctgcat gagttgtgga cgttctagca tcgctcctga ctcgttctca gtatagaatg
     7741 ctgcaatttt atggccatgc tctctagcaa aagcaataag ttcatcttta gcacgattgg
     7801 cgtcttgctc tttggttgag gcacgaaggt aggctctaat aaacataaac acccaaaata
     7861 gtctgttata agtggttcta ttataccagt ctattttagg tggtctatta atattttata
     7921 aggcgttttt aggtggttcc gctagggtat accttcactg gttcggtgca ctataagggc
     7981 atcggatagc ttagaaacca ctagaccaac aaagtttatt taattcgtac tcatctatca
     8041 aaaaatcatc aagatgatct ttaaagcctg gcttgtcact aagggtttta atagcctttt
     8101 ctgcctcttg tttagagtca taaacgccta ataatctcat gttttcggtc aaaccatcaa
     8161 tagcatgttc aagaacatat actgtcttca ttaaaaacct ccataaaaaa acatagctaa
     8221 gtaggaataa catattttat atttaaaaaa ataacggtta tcaggtatac ctataaggga
     8281 ccactcaggc cctctcactg gtttggtttc ctatataagg atctaacgtg ttccaacaat
     8341 agtaaaaatt gttaatttat atttttatgt taggtgatag gggttaatag ctttatgaga
     8401 gatgaattag aaaatactaa gtccacacag aaaaactcaa ttcatagaaa aattggttta
     8461 ggcatttata tcatatttgc tgtgatctat tattttttta tgaaagacat gtttcttgat
     8521 ggaactttct tccttatagt aggtatagcg cttcttttaa ttattatatt taaattcttt
     8581 aaagaccata cttacgattt tcaggttata gcagtttgta tgtattttct tatatttggt
     8641 tcagttcata ttctgcaatt gagtccatta attttagttg tagctggcat tgtattagta
     8701 atagttacga tatttccaca ctcaaggtca gttaaaaaag gttagtgtta ccttgattaa
     8761 ctaaaaggac aagcagtcat gaaaaagaga ctagcagtat ttttgctgac gtgtattatg
     8821 ctgacagggt gtactaaagt tattgatact cagagggaga gcactcaaaa caatgttcca
     8881 accgtaaaat ctgatgttga gaaggatttt gaaaatctca cattacaagc taatctagga
     8941 gatgcttcaa gccaagcgga attagggctt atatactact atggtagagg cgttcctcaa
     9001 gattattcaa aagcatttta ttggttacaa caagcagcta accatggaca taaagaaatg
     9061 caggggatgc tggcattgat gtatgagaag ggtcaaggcg tagatcaaaa cgatgctaaa
     9121 gcgtttgagt tatatcaaaa cttagccaat gacggtattg acttatacca atcaaaaatt
     9181 ggagatatgt accttaaagg taaaggtaca acccaaagtg atacgaaggc ttttgagtgg
     9241 acgcgcaagg cagctttgca ggatcttcca atggctcaat cagggctggc agtattctat
     9301 gagaagggta tcaatataca gcaggataaa aacaaagcgc tagaatggta tcaaaagtct
     9361 tgcagcaatg gatatccgtt tgcctgtgct gagtatcgaa gactttatca aaaatagata
     9421 tctgaaatct aaatacctcc tcaaaaatta gggttataac ctacagttcg atgtctgtat
     9481 agcgcatcga actatcggct atctctttat cagcccatta ttatcatcgg tttttaatgc
     9541 tggtacacgt tcttgtattg catgatagaa cctatctttg tcttttggtg atatcactat
     9601 gctaccacct tcatatctta tctcaatcct atctaaagac agtgctgcag cagataaagt
     9661 actgctggta ggagtgatat gggtaatgtc ttctaacgag atactctgag tagaaaaacc
     9721 atttttcact aacaatgtgt cagctgtcaa agtgtacttt atattaaaaa acagtaccaa
     9781 tagcagtaac gcagttggta tggtaaaaag gcttataaat attattcttt taacagaact
     9841 attgttgtac atccattccc atacaggcac caaaacgagt agtaagctag aacccaatat
     9901 taaaaacgcc aaccaaaaat caatctttga tgtaaatata gtatttatca taatttgtta
     9961 gcagagcgcc tttagttaat atttatatta gaatagctca gttcatgtac accaccaagt
    10021 acactgtcat aatcttgatg gctgtatcta ctatcgtata tagcccacag cctagtgttc
    10081 aagtcctcac tagggaaccg aatcagtgaa agtacctaat tggttcctta tggatatact
    10141 gcgtttatta tagatgtgtt tgtccgtagt attgttggct ggaaggtgtc taatcgtatg
    10201 aacatcgata tggtgatggc agcgttaaat caagccattg cagacagaaa caacccaaaa
    10261 gacgttattc atcgtagtga tagaggcgtt cagtatttat ctattcgtta tactgataag
    10321 atggctgatt ctggcgttat tacctttgtt ggaacgaggg taattcatat gataatgcat
    10381 agtacaattg gttatgtatc accttttgag tttgaaaagc aatattatga tcgtcttaat
    10441 gagtcaggca aagttgcctg actcaaataa aacgctctcc gatatagtcg gggcggttca
    10501 gatagccaac tggtttaata ttattccggt atatatgggg ttaaaaaacg cactaaggca
    10561 gtgattatta agagtattag aagaaggtgt tgatatttta taaatcgttc tgggataaat
    10621 aaacacagca cagatactat aaaaattcct ataatttcaa tcaaattaga tagactttga
    10681 gagccaaatc ctagcccagt taatacgacc cacaaaaatg atggtaagaa tactaggcta
    10741 aattttttca atggtttatt cataggttag gcttatcttc tatgtgggcg caatttctat
    10801 tgcaccggca acggcacaac cctgatatac ttcgcctgcg taagttaata ttgcagtaag
    10861 ttcattttga tagccattgt catcaataca aaggttattt tttatattca caacaatagg
    10921 ctgtccattg atagtactcg cgtattcgac accttcagta ttgacagaac cagaacccac
    10981 cacgataact gttgctgtcg ttggctttaa gaaattcgcc tctatactta gctcatttcc
    11041 ttcaatcact attcgccaaa ttttttcttt agcggtgttt ccaaaagcag taaatgccgc
    11101 tgacgctact ggcacttcta ttgtaggctg cttactcaga tgcgtggtgt cggcacgatg
    11161 gttattacaa gccactagag ctaagctacc cgttaagcac attgtcgtta gaagcaaaga
    11221 acgcataatt tttcctttaa atataataag agaagagggc gttcaaaact ctgtgtcaac
    11281 taaattaaag acgctagcca atttaataaa cgccccgtgc gtgattattt ggattcttga
    11341 ttggataata tttgaatatt tgccgtattt tactagcttg aacatatcat tcactggggt
    11401 ggagtgttaa tctgttatgt tttttcattg gatttgcttt ggtctattgt gtactagcta
    11461 aaatctactc gaagatatgg atgtaatata tagtttttcc gacaccaata aactcaacta
    11521 ttttggatgt taaaataatc aattattttt taatacgaaa ggttttaaga tgaaatcaaa
    11581 gaaaatactc gcccttgcat ttgtgatgtc gtgtatagct gctttagcag gctgtaacac
    11641 aacacctgat aacagtagtc agtatactaa tgcagtagag acttctcata aaaatagtca
    11701 cagtaatatt gctaaccccc aagttttgga tatcaagggc agtattactg gctatgatac
    11761 aaaggaatat atattccatg cacaagctaa tcaagtatta agagtagtaa gacaaaacac
    11821 atcagacgat gtggtattaa agttactgta tcttggaaat aacaataaaa ctagtgaact
    11881 ctctggtgat ttccaagtcc tgccttatac aggggattat aagcttatcg taagccaaac
    11941 acgcaatgat gcccgtaagc atgcaaacac cgcaaaaaac tttaatttga aagcctttat
    12001 cactgaccag cagcaaccat ctaaccagca aaattatgtc gtcaactaca agtgtgatag
    12061 caacgaaaat ctaagcgtaa cctaccactt taataaccaa aaaactagtg cgacagtgac
    12121 ttacgataat atttcacaac agctaaactt tgatgatcaa tatagtaaaa aaaataatcc
    12181 agtattctcc aataataact acttactgag cgtaggaact attaatgatg gcgtgtttgg
    12241 agactcaatg gtctactctt tgttagattt aaaaaaatct aaaggtggct acgcgttatt
    12301 gcaaaactgt caaaaattat aaacgccttc gcccatcccg aattctgtat aactgccatt
    12361 tgctaatagg gtataagcag tttaggaaag tctcttcatt agcgcctaaa agaaggcata
    12421 tatctctttg atggctctgc cttgggactt ggtgcttcct caacactgtt agtaaccttc
    12481 gctttttgta ttgccaaagg ttcgggcaaa gtagcttcct tatatcgctc ctgatgatat
    12541 tgggctttaa ttttaattcc ttcctctaaa ttcttcttat ctagttggct gcgatgaacc
    12601 ttattaggct cactatagta ggcattgctg ttaacctctt ggatatatgg ctcaaactca
    12661 acacccttat agtctttagc aaacatacca cctgtaagcc tcacactcat gaccctgcca
    12721 tttttatcct ttaggtcgtc gtgtttcact gacacactgg tttcgctatt gctccgatta
    12781 atattaagtc cccagcgcct taacgtggtc aaaagtgatt gacgattagt aactatccct
    12841 ctttgagact caagcaataa ccgccgttta agtgcctctt ctaaatcacg acggcttttg
    12901 gggtgttgaa ttatttcatc gtcttcatca ctctgttttt ttgaataact attgatatta
    12961 aaagggctag gccttggagc atggctatta tagggattaa ccaaacgttg tcttttaggg
    13021 tcgttagggt cgctcaacgg ctgttcatgt atagtcttaa tctctgcatt aactatgttt
    13081 ttccatgcat tcacccgtct ttgatccgca ggctcatagt acggctgaaa cgacttacct
    13141 gatcgtagct cttggcaggg tatgacaaag tttagctcca aacgcccaac agggttctcc
    13201 tcatcaatat ctttatcagc atgctccacc caaagaatat cgtactgatc tggctctaac
    13261 ccacaaaata tcacctgctc aaactctcgc ataatccgct ttttgtcatc ttcgctgatc
    13321 tcatcggcac gttcactaaa tgataagtag ccagacttat acttggactt atgcacagta
    13381 gtatcaatta ggtgctcagt aagcacagga tcgccgtaga gcactttcgc atgctttcga
    13441 ggcatcaacc taccatcacc tcctttctct ttaagaaggt agtttaaaac gccgctggct
    13501 ttgcctttac catgctttga gaacttaact atcacggtta tccctcacag atttttttga
    13561 ggcctctata ctcgactcga ttaacaggtg tatttgctca cgtatcgctt ttatctcatg
    13621 taatacttga gcatctacct ggttttctgt gttcacatga cgagttaact gattcaagtt
    13681 actgcctatt cgcccaagct ggcgtactaa acctggatca accggcttca caggcgtcac
    13741 tcctagagca agatctctta gccaacttgc aacagtgctc cccctgtttc tttttttaag
    13801 ttctaaaagc tcatagtcgt tcactcgaat agatatctca cgattacgtt tggctctctt
    13861 atctaaatta ataggactgt tattggacat aataagacgc accttaaaag attgaagggg
    13921 gtttggggtc tccccaacca gcacacaaga gggata
//
