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The map label for this gene is pinE [H]

Identifier: 148244056

GI number: 148244056

Start: 838

End: 1383

Strand: Reverse

Name: pinE [H]

Synonym: Acry_3553

Alternate gene names: 148244056

Gene position: 1383-838 (Counterclockwise)

Preceding gene: 148244058

Following gene: NA

Centisome position: 3.7

GC content: 68.13

Gene sequence:

>546_bases
ATGATTTACGGCTATGCACGGGTCTCCACCGACGGGCAGAGCGTGGCCGCTCAGGTCATGCAACTGAAGGCCGGCGGCTG
CGGAAAGGTCTTCCAGGAAACCGCCAGCGGCGCGCGCACTGATCGGGTCCAGCTCCGCAAGGCGATCGATCGGCTCGAGG
CCGGCGATACCCTGTTGGTCACCCGGCTCGACCGTCTCGCCCGCTCAACCCGGGACCTGCTCAACACGCTGGCGACCATC
GCCGAGAAGGGTGCCGGGTTCCGCTCCCTCAGTGACAGCTGGGCCGACACCACAACGCCGCACGGCCGGCTGATGCTCAC
CGTCCTCGGCGGCCTGGCGGAATTCGAACGGGAGCTGATCAGGGCCCGGACCGGAGAAGGAAGAGAACGGGCGAAGGCCC
GCGGTGTTCGGATGGGCCGCAAGCCGAAGCTGACCCTGCACCAGCGCCAGGAAGCCCTGCGCCGGCGCGACGCCGGCGAG
GCCGTCCGCGAGATCGCCCGCTCCTACAACGTCAGCCACAGCACGATTTCTAGGCTCGCAGACTGA

Upstream 100 bases:

>100_bases
ATATCCAGATCGGACGGTAATTCATGAATTTCGCACCAGACCCACCCCATAGGCCGGAAGCGGGGTTTTCTGTTGCATAA
GTCCGGTGCATAATATCGGC

Downstream 100 bases:

>100_bases
CGCCTTTCTGCTCCAGACTTCCGCACCAGAAAGGTGAACATGAAACCTACGGGCCGGCTCGGTGCATCACTCCTGATTTT
TAAAAAACTTTGGTTTTATT

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MIYGYARVSTDGQSVAAQVMQLKAGGCGKVFQETASGARTDRVQLRKAIDRLEAGDTLLVTRLDRLARSTRDLLNTLATI
AEKGAGFRSLSDSWADTTTPHGRLMLTVLGGLAEFERELIRARTGEGRERAKARGVRMGRKPKLTLHQRQEALRRRDAGE
AVREIARSYNVSHSTISRLAD

Sequences:

>Translated_181_residues
MIYGYARVSTDGQSVAAQVMQLKAGGCGKVFQETASGARTDRVQLRKAIDRLEAGDTLLVTRLDRLARSTRDLLNTLATI
AEKGAGFRSLSDSWADTTTPHGRLMLTVLGGLAEFERELIRARTGEGRERAKARGVRMGRKPKLTLHQRQEALRRRDAGE
AVREIARSYNVSHSTISRLAD
>Mature_181_residues
MIYGYARVSTDGQSVAAQVMQLKAGGCGKVFQETASGARTDRVQLRKAIDRLEAGDTLLVTRLDRLARSTRDLLNTLATI
AEKGAGFRSLSDSWADTTTPHGRLMLTVLGGLAEFERELIRARTGEGRERAKARGVRMGRKPKLTLHQRQEALRRRDAGE
AVREIARSYNVSHSTISRLAD

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=178, Percent_Identity=44.9438202247191, Blast_Score=138, Evalue=2e-34,
Organism=Escherichia coli, GI1787827, Length=183, Percent_Identity=34.4262295081967, Blast_Score=107, Evalue=3e-25,
Organism=Escherichia coli, GI1787638, Length=183, Percent_Identity=34.4262295081967, Blast_Score=107, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 19965; Mature: 19965

Theoretical pI: Translated: 11.35; Mature: 11.35

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYGYARVSTDGQSVAAQVMQLKAGGCGKVFQETASGARTDRVQLRKAIDRLEAGDTLLV
CCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHH
TRLDRLARSTRDLLNTLATIAEKGAGFRSLSDSWADTTTPHGRLMLTVLGGLAEFERELI
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RARTGEGRERAKARGVRMGRKPKLTLHQRQEALRRRDAGEAVREIARSYNVSHSTISRLA
HHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHC
D
C
>Mature Secondary Structure
MIYGYARVSTDGQSVAAQVMQLKAGGCGKVFQETASGARTDRVQLRKAIDRLEAGDTLLV
CCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHH
TRLDRLARSTRDLLNTLATIAEKGAGFRSLSDSWADTTTPHGRLMLTVLGGLAEFERELI
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RARTGEGRERAKARGVRMGRKPKLTLHQRQEALRRRDAGEAVREIARSYNVSHSTISRLA
HHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]