LOCUS NC_003132 9612 bp DNA circular BCT 20-JUL-2008 DEFINITION Yersinia pestis CO92 plasmid pPCP1, complete sequence. ACCESSION NC_003132 VERSION NC_003132.1 GI:16082679 DBLINK Project:34 KEYWORDS coagulase; fibrinolysin; IS100; omptin; pesticin; pesticin immunity; pim; pla; pst; rop; transcriptional regulator. SOURCE Yersinia pestis CO92 ORGANISM Yersinia pestis CO92 Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Yersinia. REFERENCE 1 (bases 1 to 9612) AUTHORS Parkhill,J., Wren,B.W., Thomson,N.R., Titball,R.W., Holden,M.T., Prentice,M.B., Sebaihia,M., James,K.D., Churcher,C., Mungall,K.L., Baker,S., Basham,D., Bentley,S.D., Brooks,K., Cerdeno-Tarraga,A.M., Chillingworth,T., Cronin,A., Davies,R.M., Davis,P., Dougan,G., Feltwell,T., Hamlin,N., Holroyd,S., Jagels,K., Karlyshev,A.V., Leather,S., Moule,S., Oyston,P.C., Quail,M., Rutherford,K., Simmonds,M., Skelton,J., Stevens,K., Whitehead,S. and Barrell,B.G. TITLE Genome sequence of Yersinia pestis, the causative agent of plague JOURNAL Nature 413 (6855), 523-527 (2001) PUBMED 11586360 REFERENCE 2 (bases 1 to 9612) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (10-SEP-2004) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL109969. COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..9612 /organism="Yersinia pestis CO92" /mol_type="genomic DNA" /strain="CO92" /db_xref="taxon:214092" /plasmid="pPCP1" /biovar="Orientalis" misc_feature 1..9612 /note="REFSEQ gene predictions performed by GeneMark 2.4/GeneMark.hmm 2.0 with comparison to original submitter provided annotation" repeat_region 1..1954 /mobile_element="insertion sequence:IS100" gene 87..1109 /locus_tag="YPPCP1.01" /db_xref="GeneID:1172637" CDS 87..1109 /locus_tag="YPPCP1.01" /note="probable IS100 transposase; similar to TR:P74993; similar to TRA6_BACST putative transposase for insertion sequence element IS5376; contains helix-turn-helix motif from aa 19-40" /codon_start=1 /transl_table=11 /product="putative transposase" /protein_id="NP_395227.1" /db_xref="GI:16082680" /db_xref="GeneID:1172637" /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS FCRGVA" gene 1106..1888 /locus_tag="YPPCP1.02" /db_xref="GeneID:1172638" CDS 1106..1888 /locus_tag="YPPCP1.02" /note="IS100 ATP-binding protein; similar to TR:P74994; similar to ISTB_ECOLI insertion sequence IS21 putative ATP-binding protein; contains PS00017 ATP/GTP-binding site motif A; P-loop" /codon_start=1 /transl_table=11 /product="transposase/IS protein" /protein_id="NP_395228.1" /db_xref="GI:16082681" /db_xref="GeneID:1172638" /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER NENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQ RGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD AALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE" repeat_region 2265..2283 /note="rep" repeat_region 2321..2340 /note="rep" repeat_region complement(2413..2432) /note="rep" rep_origin 2924..4176 /note="similar to ColE1 ori" gene 2925..3119 /gene="rop" /locus_tag="YPPCP1.03" /db_xref="GeneID:1172639" CDS 2925..3119 /gene="rop" /locus_tag="YPPCP1.03" /note="plasmid replication regulatory protein; similar to ROP_ECOLI regulatory protein ROP from ColE1; RNA one modulator" /codon_start=1 /transl_table=11 /product="putative replication regulatory protein" /protein_id="NP_395229.1" /db_xref="GI:16082682" /db_xref="GeneID:1172639" /translation="MNKQQQTALNMARFIRSQSLILLEKLDALDADEQAAMCERLHEL AEELQNSIQARFEAESETGT" gene 4355..4780 /gene="pim" /locus_tag="YPPCP1.04" /db_xref="GeneID:1172640" CDS 4355..4780 /gene="pim" /locus_tag="YPPCP1.04" /note="pesticin immunity protein; similar to TR:Q57285 (EMBL:Z54145; X92856)" /codon_start=1 /transl_table=11 /product="pesticin immunity protein" /protein_id="NP_395230.1" /db_xref="GI:16082683" /db_xref="GeneID:1172640" /translation="MISKLFCLALIFLSSSGLAEKNTYTAKDILQNLELNTFGNSLSH GIYGKQTTFKQTEFTNIKSNTKKHIALINKDNSWMISLKILGIKRDEYTVCFEDFSLI RPPTYVAIHPLLIKKVKSGNFIVVKEIKKSIPGCTVYYH" gene complement(4815..5888) /gene="pst" /locus_tag="YPPCP1.05c" /db_xref="GeneID:1172641" CDS complement(4815..5888) /gene="pst" /locus_tag="YPPCP1.05c" /note="similar to TR:Q57159 (EMBL:X92856)" /codon_start=1 /transl_table=11 /product="pesticin" /protein_id="NP_395231.1" /db_xref="GI:16082684" /db_xref="GeneID:1172641" /translation="MSDTMVVNGSGGVPAFLFSGSTLSSYRPNFEANSITIALPHYVD LPGRSNFKLMYIMGFPIDTEMEKDSEYSNKIRQESKISKTEGTVSYEQKITVETGQEK DGVKVYRVMVLEGTIAESIEHLDKKENEDILNNNRNRIVLADNTVINFDNISQLKEFL RRSVNIVDHDIFSSNGFEGFNPTSHFPSNPSSDYFNSTGVTFGSGVDLGQRSKQDLLN DGVPQYIADRLDGYYMLRGKEAYDKVRTAPLTLSDNEAHLLSNIYIDKFSHKIEGLFN DANIGLRFSDLPLRTRTALVSIGYQKGFKLSRTAPTVWNKVIAKDWNGLVNAFNNIVD GMSDRRKREGALVQKDIDSGLLK" variation 5910 /note="as compared to sequence found in GenBank Accession Number AF053945" /replace="" variation 5927 /note="as compared to sequence found in GenBank Accession Number AF053945" /replace="aa" gene 6006..6422 /locus_tag="YPPCP1.06" /db_xref="GeneID:1172642" CDS 6006..6422 /locus_tag="YPPCP1.06" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="NP_395232.1" /db_xref="GI:16082685" /db_xref="GeneID:1172642" /translation="MKFHFCDLNHSYKNQEGKIRSRKTAPGNIRKKQKGDNVSKTKSG RHRLSKTDKRLLAALVVAGYEERTARDLIQKHVYTLTQADLRHLVSEISNGVGQSQAY DAIYQARRIRLARKYLSGKKPEGVEPREGQEREDLP" gene 6665..7603 /gene="pla" /locus_tag="YPPCP1.07" /db_xref="GeneID:1172643" CDS 6665..7603 /gene="pla" /locus_tag="YPPCP1.07" /EC_number="3.4.23.48" /note="outer membrane protease; involved in virulence in many organisms; OmpT; IcsP; SopA; Pla; PgtE; omptin; in Escherichia coli OmpT can degrade antimicrobial peptides; in Yersinia Pla activates plasminogen during infection; in Shigella flexneria SopA cleaves the autotransporter IcsA" /codon_start=1 /transl_table=11 /product="outer membrane protease" /protein_id="NP_395233.1" /db_xref="GI:16082686" /db_xref="GeneID:1172643" /translation="MKKSSIVATIITILSGSANAASSQLIPNISPDSFTVAASTGMLS GKSHEMLYDAETGRKISQLDWKIKNVAILKGDISWDPYSFLTLNARGWTSLASGSGNM DDYDWMNENQSEWTDHSSHPATNVNHANEYDLNVKGWLLQDENYKAGITAGYQETRFS WTATGGSYSYNNGAYTGNFPKGVRVIGYNQRFSMPYIGLAGQYRINDFELNALFKFSD WVRAHDNDEHYMRDLTFREKTSGSRYYGTVINAGYYVTPNAKVFAEFTYSKYDEGKGG TQTIDKNSGDSVSIGGDAAGISNKNYTVTAGLQYRF" gene complement(7790..8089) /locus_tag="YPPCP1.08c" /db_xref="GeneID:1172644" CDS complement(7790..8089) /locus_tag="YPPCP1.08c" /note="similar to predicted transcriptional regulator TR:P95258 (EMBL:Z84498) Mycobacterium tuberculosis MTCY09F9.0 8C (Rv1956); contains helix-turn-helix motif from aa 42-63" /codon_start=1 /transl_table=11 /product="putative transcriptional regulator" /protein_id="NP_395234.1" /db_xref="GI:16082687" /db_xref="GeneID:1172644" /translation="MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQK QVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMGGKLSLDVELPTGRRVAFHV" gene complement(8089..8436) /locus_tag="YPPCP1.09c" /db_xref="GeneID:1172645" CDS complement(8089..8436) /locus_tag="YPPCP1.09c" /note="similar to two hypothetical proteins from Mycobacterium tuberculosis TR:O53468 (EMBL:O53468) MTV018.09C (Rv2022c); similar to TR:O53332 (EMBL:AL021646) MTV014.26 (Rv3182)" /codon_start=1 /transl_table=11 /label=YPPCP1.09c /product="hypothetical protein" /protein_id="NP_395235.1" /db_xref="GI:16082688" /db_xref="GeneID:1172645" /translation="MWMVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYA DTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAIVLCAGDKSNDKRFYEKLVRIAE DEFTAHLNTLESK" variation 8528..8529 /note="as compared to sequence found in GenBank Accession Number AF053945" /replace="" ORIGIN 1 tgtaacgaac ggtgcaatag tgatccacac ccaacgcctg aaatcagatc cagggggtaa 61 tctgctctcc tgattcagga gagtttatgg tcacttttga gacagttatg gaaattaaaa 121 tcctgcacaa gcagggaatg agtagccggg cgattgccag agaactgggg atctcccgca 181 ataccgttaa acgttatttg caggcaaaat ctgagccgcc aaaatatacg ccgcgacctg 241 ctgttgcttc actcctggat gaataccggg attatattcg tcaacgcatc gccgatgctc 301 atccttacaa aatcccggca acggtaatcg ctcgcgagat cagagaccag ggatatcgtg 361 gcggaatgac cattctcagg gcattcattc gttctctctc ggttcctcag gagcaggagc 421 ctgccgttcg gttcgaaact gaacccggac gacagatgca ggttgactgg ggcactatgc 481 gtaatggtcg ctcaccgctt cacgtgttcg ttgctgttct cggatacagc cgaatgctgt 541 acatcgaatt cactgacaat atgcgttatg acacgctgga gacctgccat cgtaatgcgt 601 tccgcttctt tggtggtgtg ccgcgcgaag tgttgtatga caatatgaaa actgtggttc 661 tgcaacgtga cgcatatcag accggtcagc accggttcca tccttcgctg tggcagttcg 721 gcaaggagat gggcttctct ccccgactgt gtcgcccctt cagggcacag actaaaggta 781 aggtggaacg gatggtgcag tacacccgta acagttttta catcccacta atgactcgcc 841 tgcgcccgat ggggatcact gtcgatgttg aaacagccaa ccgccacggt ctgcgctggc 901 tgcacgatgt cgctaaccaa cgaaagcatg aaacaatcca ggcccgtccc tgcgatcgct 961 ggctcgaaga gcagcagtcc atgctggcac tgcctccgga gaaaaaagag tatgacgtgc 1021 atcttgatga aaatctggtg aacttcgaca aacaccccct gcatcatcca ctctccatct 1081 acgactcatt ctgcagagga gtggcgtgat gatggaactg caacatcaac gactgatggc 1141 gctcgccggg cagttgcaac tggaaagcct tataagcgca gcgcctgcgc tgtcacaaca 1201 ggcagtagac caggaatgga gttatatgga cttcctggag catctgcttc atgaagaaaa 1261 actggcacgt catcaacgta aacaggcgat gtatacccga atggcagcct tcccggcggt 1321 gaaaacgttc gaagagtatg acttcacatt cgccaccgga gcaccgcaga agcaactcca 1381 gtcgttacgc tcactcagct tcatagaacg taatgaaaat atcgtattac tggggccatc 1441 aggtgtgggg aaaacccatc tggcaatagc gatgggctat gaagcagtcc gtgcaggtat 1501 caaagttcgc ttcacaacag cagcagatct gttacttcag ttatctacgg cacaacgtca 1561 gggccgttat aaaacgacgc ttcagcgtgg agtaatggcc ccccgcctgc tcatcattga 1621 tgaaataggc tatctgccgt tcagtcagga agaagcaaag ctgttcttcc aggtcatcgc 1681 taaacgttac gaaaagagcg caatgatcct gacatccaat ctgccgttcg ggcagtggga 1741 tcaaacgttc gccggtgatg cagcactgac ctcagcgatg ctggaccgta tcttacacca 1801 ctcacatgtc gttcaaatca aaggagaaag ctatcgactc agacagaaac gaaaggccgg 1861 ggttatagca gaagctaatc ctgagtaaaa cggtggatca atattgggcc gttggtggag 1921 atataagtgg atcacttttc atccgtcgtt gacaccctga tgaattcacg tgttcacgcc 1981 tgaataacaa gaatgccgga gatacgcagt catatttttt acacaattct ctaatcccga 2041 caaggtcgta ggtcgttata ggaaaattct tagcaccatt ccggaacaat cagaacagca 2101 ggccatgaac gactgacaac attacgaata taaaaaacgc acccgggcca gacattcccc 2161 ctactgatta aaccagccgg acttgtccac ggaacggtct ttttaaaccg acacacagtc 2221 tgagtacaga tacatgtcac gatgatgcag gattagcgga agagtgtgag cacgtttccg 2281 ggaactgtgg tgaaccatag ctcaatattc gagtgagggc ataccggaaa cgcgctcaga 2341 ttcgttgtaa cgcgattttc cgtaccgggc aattttttca gttgtttttt cgtttcatgt 2401 cgtcagaaac gttctgagcg cgtttccggc atctgatgct acgcaaacca tccccatggt 2461 cagttgacag ccggaaacac gcgggtgtcg ttttagcgta tcgacgggac ggcgtcgaga 2521 agcacaaaaa acagatgttg tactcagtca gttgttttac agacagcact gcggcagatt 2581 gaaaaagtac cgtactttca ggaatgtcca gaaaccatgt gtcagacttc gttctccccc 2641 ttccgggtga atttttttgt catccgttca ggaatctctt tataacgatt actccatttc 2701 aggatttttt atgtggcgtt tactacaggc aggatattca aaggcaaaaa aatcccccgg 2761 aacaggcgga acccggacag ggggagaacg aatcgctaaa taattttcgt agttgtattt 2821 cccatcgttg ctactgcaac gggatgaatt tgccgcagtt tatcctgtaa aacaatcctg 2881 atttactcac actccacata tcactgacgg agcacaacgg aatagtgaac aaacaacaac 2941 aaactgcgct gaatatggcg cgatttatca gaagccagag cctgatactg cttgaaaaac 3001 tggatgctct ggatgccgac gagcaggcgg ccatgtgtga acgactgcac gaactcgcgg 3061 aagaactcca gaacagcatc caggctcgct ttgaagccga aagtgaaaca ggaacataac 3121 gaagctcccg gagacggtca cagcttgtct gtgaacggat gccgggagca gacaagcccg 3181 tcagggcgcg tcagcgggtt ttagcgggtg tcggggcgca gccatgaccc agtcacgtag 3241 cgatagcgga gtgtatactg gcttagtcat gcggcatcag tgcggattgt atgaaaagtg 3301 caccatgtac ggtgtgaaat gccgcacaga tgcgtaagga gaacatgcag atgccgatgc 3361 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggtg 3421 tctgctcact caaaagcggt gatactgtta tccacacaat caggggataa cgccggaaag 3481 aacatgtgag caaaaaacga agaccccaga aaaggccgcg ccggaggcgc tttttccata 3541 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 3601 cgacaggact taaagatacc aggcgtttcc ccccggaagc tccctcgtgc gctctcctgt 3661 tccgaccctg ccgcttaccg gatacctctc cgcctttctc ccttcgggaa gcgtggcgct 3721 ttctcatagc tcacgctgtt ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 3781 ctgtgtgcac gaaccccccg ttcagcccga ccactgcgcc ttatccggta actatcgtct 3841 tgagtccaac ccggtaagac acgactttac gccactggca gcagccattg gtaactgaaa 3901 agtggattta gatacgcaga actcttgaag ttgaagcctt atcgcggcta cactgaaagg 3961 acagcatttg gtatctgtgc tccacttaag ccagctacca caggttagaa agcctgagaa 4021 acttctaacc ttcgaaagaa cccacgcctg agaacgtggg ttttttcgtt tacaggcagc 4081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct actgaattgc 4141 gctcccgatc agttcagcag aagattatga tggggttcta tgggtattgc tgcggtaaca 4201 cccatgttac ttgaggttgt atgtagtctg tgtagaatta tacacataag gcttaaactg 4261 ctcttttttt tcaatatgca attggaagtt cattgactac ataaatagat tattccaaat 4321 aatttattta tgtaagaaca ggatgggagg gggaatgatc tcaaagttat tttgcttggc 4381 tctcatattt ttatcatcaa gtggccttgc agaaaaaaac acatatacag caaaagacat 4441 cttgcaaaac ctagaattaa atacctttgg caattcattg tctcatggca tctatgggaa 4501 acagacaacc ttcaagcaaa ccgagtttac aaatattaaa agcaacacca aaaaacacat 4561 tgcacttatc aataaagaca actcatggat gatatcatta aaaatactag gaattaagag 4621 agatgagtat actgtctgtt ttgaagattt ctctctaata agaccgccaa catatgtagc 4681 catacatcct ctacttataa aaaaagtaaa atctggaaac tttatagtag tgaaagaaat 4741 aaagaaatct atccctggtt gcactgtata ttatcattaa tagcaagccc ctcattatta 4801 tgaggggctc atggttattt taacaatcca ctatcgatat ctttttgcac cagagcgccc 4861 tctcgtttac gtctgtcaga cattccatca acaatattat taaaagcatt tacaaggcca 4921 ttccagtctt ttgcgataac tttattccat actgtgggag cagttctgga taacttaaac 4981 cctttttgat atccaataga caccagtgct gtacgggttc tcaacggtaa atcgctgaac 5041 cgaagaccga tattagcgtc attgaaaaga ccttcaatct tatgtgagaa tttatcaata 5101 taaatattag ataagagatg agcttcatta tcagaaagcg tcagaggtgc tgttctcact 5161 ttatcataag cctccttccc tcgaagcata taatacccat caagtctatc tgcaatatac 5221 tgagggacac cgtcattcaa taaatcctgt ttgcttcgct gaccaaggtc aaccccggaa 5281 ccgaatgtaa caccggtact gttaaaataa tcgctactag gattagacgg aaaatgactt 5341 gtcggattaa acccttcaaa accattactg gagaaaatat cgtggtcaac aatatttacc 5401 gaacgacgta aaaattcctt cagttgacta atattgtcaa agttaatgac agtgttgtcc 5461 gctaggacga tgcgatttcg gttattattc agaatgtctt cgttctcttt cttatcgaga 5521 tgttcaatag attcggcaat cgttccctca agaaccatga cacggtagac tttcacaccg 5581 tctttttcct gacctgtttc aacagttatt ttctgttcgt aagacacggt cccttcagtt 5641 tttgaaattt tactttcctg gcggatctta tttgaatatt cactgtcttt ctccatctcc 5701 gtatcaatcg gaaaccccat aatgtacatc agtttaaaat tactccggcc aggcagatcc 5761 acataatgtg gtaatgcaat tgtaatcgaa ttagcttcaa aatttggtct gtaactgctt 5821 aatgtacttc cggaaaagag aaaagccgga acaccacctg aaccattcac taccattgta 5881 tctgacataa aaattcctct ttaacacata aaaaaaacaa taagttaaaa aaaatactgt 5941 acataaaacc actgttttta tgtacagtaa taaaattacg ccgctttatt ttctctgtca 6001 ataatatgaa atttcatttt tgtgatctga atcactctta taaaaatcag gaagggaaga 6061 ttcgcagcag aaaaacagca ccgggtaaca tcagaaaaaa acagaaagga gataacgtga 6121 gcaaaacaaa atctggtcgc caccgactga gcaaaacaga caaacgcctg ctggctgcac 6181 ttgtcgttgc cggatacgaa gaacggacag cccgtgacct catccagaaa cacgtttaca 6241 cactgacaca ggccgacctg cgccatctgg tcagtgaaat cagtaacggt gtgggacagt 6301 cacaggccta cgatgcgatt taccaggcga gacgcattcg tctcgcccgt aaatacctga 6361 gcggaaaaaa accggaaggg gtggaacccc gggaagggca ggaacgggaa gatttaccat 6421 aactcccgtt atcagtacca tcggctcaac gctcgttgtc ggatctgaaa aattcgctca 6481 aaagatcata tttccctgga tattttccac cgtttcttat gtgagcaaag tcacataatt 6541 ctgtcagacg acgagaaaac ggatatcgat tattgtttaa tatttttaca ttattaaaaa 6601 tgaaattaga taatcagata caaataatat gttttcgttc atgcagagag attaagggtg 6661 tctaatgaag aaaagttcta ttgtggcaac cattataact attctgtccg ggagtgctaa 6721 tgcagcatca tctcagttaa taccaaatat atcccctgac agctttacag ttgcagcctc 6781 caccgggatg ctgagtggaa agtctcatga aatgctttat gacgcagaaa caggaagaaa 6841 gatcagccag ttagactgga agatcaaaaa tgtcgctatc ctgaaaggtg atatatcctg 6901 ggatccatac tcatttctga ccctgaatgc cagggggtgg acgtctctgg cttccgggtc 6961 aggtaatatg gatgactacg actggatgaa tgaaaatcaa tctgagtgga cagatcactc 7021 atctcatcct gctacaaatg ttaatcatgc caatgaatat gacctcaatg tgaaaggctg 7081 gttactccag gatgagaatt ataaagcagg tataacagca ggatatcagg aaacacgttt 7141 cagttggaca gctacaggtg gttcatatag ttataataat ggagcttata ccggaaactt 7201 cccgaaagga gtgcgggtaa taggttataa ccagcgcttt tctatgccat atattggact 7261 tgcaggccag tatcgcatta atgattttga gttaaatgca ttatttaaat tcagcgactg 7321 ggttcgggca catgataatg atgagcacta tatgagagat cttactttcc gtgagaagac 7381 atccggctca cgttattatg gtaccgtaat taacgctgga tattatgtca cacctaatgc 7441 caaagtcttt gcggaattta catacagtaa atatgatgag ggcaaaggag gtactcagac 7501 cattgataag aatagtggag attctgtctc tattggcgga gatgctgccg gtatttccaa 7561 taaaaattat actgtgacgg cgggtctgca atatcgcttc tgaaaaatac agatcatatc 7621 tctcttttca tcctccccta gcggggagga tgtctgtgga aaggaggttg gtgtttgacc 7681 aaccttcaga tgtgtgaaaa atcacctttt tcaccataat gacggggcgc tcattctgtt 7741 gttttgcctt gacattctcc acgtctttca gggcatggag aaggtcaaat tagacatgga 7801 acgctactct ccttcctgta ggaagctcaa catccaagct taatttgcct cccattgctt 7861 caacgtaacg ctttaacgtc gccagcttta aatcatttcc gcgctgctcc agctttgtta 7921 ctgctggctg gcttataccc atcgcctcag caacttgttt ttgtgataac tggagttctt 7981 cacgcatcat ctgcaagccg acctcaagaa tcatctcatc tgccatttct ttaattcgtg 8041 tctggctttc aggtgaacga ctggcaatca cctcatctaa tgttctcatt acttgctctc 8101 cagtgtgttc agatgtgctg taaattcatc ctcagctata cgcaccagtt tttcataaaa 8161 ccgcttatca ttacttttat ctcctgcaca aagaacgata gcccgacgaa tcggatcgaa 8221 cgcataaaag gctcttatcg gacggccaga aaactgaacg cgaagctctt tcatattttt 8281 gtaccgagaa cctttcacgg tatcggcata tggcctgggt aactcaggtc cgtaaacctg 8341 tagctttttc aaatcagcca aaaccttttc ctgaagagcg tcttcttgct catttagcca 8401 gtcgtcaaat cgctggctaa aaagtaccat ccacatgctc aaccctataa cctgtagctt 8461 accccactaa caatataacc tacgagttat attttcaaga aaagctggct atttaacata 8521 acggcaattt ttgtacgcac cactgaaatg cgttcagcgc gatcacggca acagacaggc 8581 aaaaatagca acaaacctcc cgaaaaaccg ccgcgatcgc gcctgataaa ttttaacctt 8641 atgcatatct atgcagccag gcgaatcacg aacgaattgc ctgcctgatg taactgaaac 8701 gggtgttttt tcctgatttg gtgggcgtgg aagacggaac atgaacggga aaacagaatt 8761 catgccagat gagcgcgatc tggcaattaa ggcaaaacac agcaacaaag acacgccaga 8821 atcgcgcccg gatatgtttt aacgcgattt tcagactcag acaaattcag cagaatgcta 8881 ctccattcac cgggctgatg gtgaatacat gcgtatccag gatgagtaca tttctggctc 8941 tgccacagct ctgtctgttg gcagctttcg cctgtccgga aacctgctta aaacgctccc 9001 gaaaggcctc tgaaccagaa agcaacaaaa cacaggccat taagtaaatc gcgttaaaac 9061 acgtctgatg gattgctgca aaaaaaagtc cctaatggag cagggactgt taaacccagt 9121 gaatagcgtc taaattaaag taagaatacg accaggtact cttcagaaaa gagattaatc 9181 caccgcacag aataatcaac agtaaaaaca aacaaccctg attttttatt tttctttttt 9241 tcgataaaaa caaaattaaa gaaataatta atcagaacat tccttaactt cagggcattg 9301 cctgtgttcc attttgtgat tagtctgaaa cttccgaagg tggataacac ccggtatttt 9361 tttgctcaca taaagcccct ccttcaggca gaggggcttt ttctttgcca ccacataaaa 9421 aaggccctca caggaggtgt tctgtgaggg cgtatgataa ggactgaatc gatggttaat 9481 atgtctagtc ctgacttttg catctccgaa tataaaaccc tgtttaacgg catgcaaaac 9541 caaaaaataa aaatgtgaca tcgcaatgcc agataatatt gacgcatgag ggaatgcgta 9601 ccccgacccc tg //