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The map label for this gene is appB [H]

Identifier: 38234676

GI number: 38234676

Start: 2179481

End: 2180479

Strand: Reverse

Name: appB [H]

Synonym: DIP2127

Alternate gene names: 38234676

Gene position: 2180479-2179481 (Counterclockwise)

Preceding gene: 38234677

Following gene: 38234675

Centisome position: 87.62

GC content: 56.46

Gene sequence:

>999_bases
GTGGCTGCGTTATTGCACCGTAGGGATAATCTCCACGGAGTCACCGCGATGGTGGGCTGGCGAATGCTCACCATCGCGGT
GACCACCGTGGTGGTTACGTTTATCATGTTCGGCCTTGCGGCACTGTCGCCGTTTGATCCCCTAGCGCACTATCTCGGTT
CGAACTATGGCGACTACACCGAGGCGGAACGAGCAAAAATTGCGGCAGCCCTCGGAGTGGATGTTCCGTGGTACCACCAG
TGGTTGCGCTGGTGGACAGACGTGTTGACCGGTGATCTTGGCTGGTCGCGGGTGTACAACAAACCAGTTTCTGCTGTGAT
CGTCGAACGACTCCCATGGACGATCCTGCTATCAGCTACTGGGTTGCTCCTCATGCTAGTGATTGCGACGATCCTCGGAG
TATGGTCGGCGCGCCGACCTGGGGGTGTCGTCGATAAGGCGGTGAATGCCCTAGGCGTGTTCATCGCGGCAACCCCCTCC
TACGTGTACGCACTCGGTACAGTCTTGCTGTTTGCGATTTTTCTCCACGCCATCCCGGTCGGTGGCGCAAGCCCCGTCGG
ATACGCACCTCGACTGGCAACCGTCGGCCCCTACCTCATAGCGCCTGCCATAGTGTTAGCCATATCGCAGCTATCGTGGC
CGCTGCTCACAATGCAACAAGCCACCGTAGAAGCCACCCGTTCACCAGCGGTAGCAAATGCGCGTCTGCGCGGCATTAAA
GAAAGAACTGTGCTGATAAAGCATGTTCTTCCCATGTCTATGATGCCGTTGGTAACACTATTGGGTGCCCGTCTAGGCGA
ACTCGTGGTCGGTGCGGTGATCGTCGAAACGGTCTTTTCGTGGCCAGGGCTTGCTCAAGCAACCGTGGAATCCGCTATCG
CAGTAGATTTTCCCCTCTTAGCTTTTATTACCGTTGCCACCACAGTTGTGGTCATGCTGGGATCTCTTGCAAGCGACCTG
TCCTATATGTTGATCGATCCGAGGGTAAGCGATGTCTAA

Upstream 100 bases:

>100_bases
CAAGAAGAACGATTGGAAGAAACCAGAACTTCTTCTTGAGCCTCACATCCACGGTGTGACCTGGGGTCCATGGTGGCGGA
TCTCGGAGTGGACAAAATAA

Downstream 100 bases:

>100_bases
CGCTCAGTACATGTCGTGGGCCAAACAACACACAGTAAGCTTGGCAGTCTTCTTGCTCGTGCTGCTGTATGCACTGATTG
TGCCCCTAGTAATGGATGCC

Product: putative binding-protein-dependent integral membrane transport protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MAALLHRRDNLHGVTAMVGWRMLTIAVTTVVVTFIMFGLAALSPFDPLAHYLGSNYGDYTEAERAKIAAALGVDVPWYHQ
WLRWWTDVLTGDLGWSRVYNKPVSAVIVERLPWTILLSATGLLLMLVIATILGVWSARRPGGVVDKAVNALGVFIAATPS
YVYALGTVLLFAIFLHAIPVGGASPVGYAPRLATVGPYLIAPAIVLAISQLSWPLLTMQQATVEATRSPAVANARLRGIK
ERTVLIKHVLPMSMMPLVTLLGARLGELVVGAVIVETVFSWPGLAQATVESAIAVDFPLLAFITVATTVVVMLGSLASDL
SYMLIDPRVSDV

Sequences:

>Translated_332_residues
MAALLHRRDNLHGVTAMVGWRMLTIAVTTVVVTFIMFGLAALSPFDPLAHYLGSNYGDYTEAERAKIAAALGVDVPWYHQ
WLRWWTDVLTGDLGWSRVYNKPVSAVIVERLPWTILLSATGLLLMLVIATILGVWSARRPGGVVDKAVNALGVFIAATPS
YVYALGTVLLFAIFLHAIPVGGASPVGYAPRLATVGPYLIAPAIVLAISQLSWPLLTMQQATVEATRSPAVANARLRGIK
ERTVLIKHVLPMSMMPLVTLLGARLGELVVGAVIVETVFSWPGLAQATVESAIAVDFPLLAFITVATTVVVMLGSLASDL
SYMLIDPRVSDV
>Mature_331_residues
AALLHRRDNLHGVTAMVGWRMLTIAVTTVVVTFIMFGLAALSPFDPLAHYLGSNYGDYTEAERAKIAAALGVDVPWYHQW
LRWWTDVLTGDLGWSRVYNKPVSAVIVERLPWTILLSATGLLLMLVIATILGVWSARRPGGVVDKAVNALGVFIAATPSY
VYALGTVLLFAIFLHAIPVGGASPVGYAPRLATVGPYLIAPAIVLAISQLSWPLLTMQQATVEATRSPAVANARLRGIKE
RTVLIKHVLPMSMMPLVTLLGARLGELVVGAVIVETVFSWPGLAQATVESAIAVDFPLLAFITVATTVVVMLGSLASDLS
YMLIDPRVSDV

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=309, Percent_Identity=27.5080906148867, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1789965, Length=331, Percent_Identity=25.0755287009063, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787761, Length=330, Percent_Identity=30.9090909090909, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787053, Length=313, Percent_Identity=25.8785942492013, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787497, Length=303, Percent_Identity=24.7524752475248, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788504, Length=274, Percent_Identity=23.7226277372263, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787550, Length=271, Percent_Identity=21.4022140221402, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35636; Mature: 35504

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
>Mature Secondary Structure 
AALLHRRDNLHGVTAMVGWRMLTIAVTTVVVTFIMFGLAALSPFDPLAHYLGSNYGDYT
CCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCH
EAERAKIAAALGVDVPWYHQWLRWWTDVLTGDLGWSRVYNKPVSAVIVERLPWTILLSAT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHCCHHHHHHHH
GLLLMLVIATILGVWSARRPGGVVDKAVNALGVFIAATPSYVYALGTVLLFAIFLHAIPV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
GGASPVGYAPRLATVGPYLIAPAIVLAISQLSWPLLTMQQATVEATRSPAVANARLRGIK
CCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
ERTVLIKHVLPMSMMPLVTLLGARLGELVVGAVIVETVFSWPGLAQATVESAIAVDFPLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AFITVATTVVVMLGSLASDLSYMLIDPRVSDV
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]