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The map label for this gene is grpE

Identifier: 38234668

GI number: 38234668

Start: 2170135

End: 2170794

Strand: Reverse

Name: grpE

Synonym: DIP2119

Alternate gene names: 38234668

Gene position: 2170794-2170135 (Counterclockwise)

Preceding gene: 38234669

Following gene: 38234667

Centisome position: 87.23

GC content: 58.79

Gene sequence:

>660_bases
ATGAGCACTACCGACAACACTAACGGCGACGACCGCCGCCCAGGCCAACCGGAATGGGATGACGAGGAGAACAACTTCGA
GCATCTTGATGCCACCGAAGTCCCCGCCGAGGAGGAAGCCTTGGTGGAGTCGGCTGGTCTTGATGCTGATACCCTCGCGG
ATCTCGAAGACGCCTTCGACGGTGTCGACGCCTCCACCGAGGATCCAGGTGCTACCGTCGGCGAGACCTCGACTCTTGAG
TCAGAGCTCGCCGAGCGTACCGAGGATCTGCAGCGCCTTAGCGCCGAATACGCTAACTATCGACGCCGCACCGACCGCGA
ACGCAAGGTCGGCGTCGAGGCCGCGAAGGCCAAGGTTCTCGGCGAGTTGCTTCCCATATTGGATGATCTGGAGCTTGCGC
AAAAACACGGTGACCTCGACGAAGGACCTCTCAAAGCATTCCGCGACAAGTTAGTGAGCGTTGTTGAAGGTTTGGGCGTA
TCCGCATTCGGTGCCGAGGGCGACGTGTTTGATGCCGAGCGCCATGAGGCAGTTCAGGATCTCTCTTCGGGAGATGACAA
GGTGCTGGGAACTGTCCTGCGCCGTGGCTATCAGATGAACGATCGTTTGCTGCGAACCGCAATGGTGATCATTGCCGATC
CAGCAGAAGATGCTCAATAG

Upstream 100 bases:

>100_bases
CTGAGGCCGCTAACGCTGGCGCTGCTGATGCCACCAACGTTGATCCTAACGTCGTCGACGCTGAGGTCGTTGAGGACGAT
GACAAGGACGGAAACAAGTA

Downstream 100 bases:

>100_bases
TCACTGAGTTTCACTGAGATGAATGCGGGATCCAGCAGCCCACGGTGGTGGGGAGCTGGGTTCCGCATTTTCAACTAAAT
ACGGAAAACAAGAAAGGAGG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSTTDNTNGDDRRPGQPEWDDEENNFEHLDATEVPAEEEALVESAGLDADTLADLEDAFDGVDASTEDPGATVGETSTLE
SELAERTEDLQRLSAEYANYRRRTDRERKVGVEAAKAKVLGELLPILDDLELAQKHGDLDEGPLKAFRDKLVSVVEGLGV
SAFGAEGDVFDAERHEAVQDLSSGDDKVLGTVLRRGYQMNDRLLRTAMVIIADPAEDAQ

Sequences:

>Translated_219_residues
MSTTDNTNGDDRRPGQPEWDDEENNFEHLDATEVPAEEEALVESAGLDADTLADLEDAFDGVDASTEDPGATVGETSTLE
SELAERTEDLQRLSAEYANYRRRTDRERKVGVEAAKAKVLGELLPILDDLELAQKHGDLDEGPLKAFRDKLVSVVEGLGV
SAFGAEGDVFDAERHEAVQDLSSGDDKVLGTVLRRGYQMNDRLLRTAMVIIADPAEDAQ
>Mature_218_residues
STTDNTNGDDRRPGQPEWDDEENNFEHLDATEVPAEEEALVESAGLDADTLADLEDAFDGVDASTEDPGATVGETSTLES
ELAERTEDLQRLSAEYANYRRRTDRERKVGVEAAKAKVLGELLPILDDLELAQKHGDLDEGPLKAFRDKLVSVVEGLGVS
AFGAEGDVFDAERHEAVQDLSSGDDKVLGTVLRRGYQMNDRLLRTAMVIIADPAEDAQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

None

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_CORDI (Q6NEZ0)

Other databases:

- EMBL:   BX248360
- RefSeq:   NP_940435.1
- ProteinModelPortal:   Q6NEZ0
- SMR:   Q6NEZ0
- GeneID:   2649586
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP2119
- NMPDR:   fig|257309.1.peg.2026
- HOGENOM:   HBG732630
- OMA:   SAEYANY
- ProtClustDB:   PRK14164
- BioCyc:   CDIP257309:DIP2119-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 23890; Mature: 23759

Theoretical pI: Translated: 3.93; Mature: 3.93

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTDNTNGDDRRPGQPEWDDEENNFEHLDATEVPAEEEALVESAGLDADTLADLEDAFD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHC
GVDASTEDPGATVGETSTLESELAERTEDLQRLSAEYANYRRRTDRERKVGVEAAKAKVL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GELLPILDDLELAQKHGDLDEGPLKAFRDKLVSVVEGLGVSAFGAEGDVFDAERHEAVQD
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
LSSGDDKVLGTVLRRGYQMNDRLLRTAMVIIADPAEDAQ
HCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
STTDNTNGDDRRPGQPEWDDEENNFEHLDATEVPAEEEALVESAGLDADTLADLEDAFD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHC
GVDASTEDPGATVGETSTLESELAERTEDLQRLSAEYANYRRRTDRERKVGVEAAKAKVL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GELLPILDDLELAQKHGDLDEGPLKAFRDKLVSVVEGLGVSAFGAEGDVFDAERHEAVQD
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
LSSGDDKVLGTVLRRGYQMNDRLLRTAMVIIADPAEDAQ
HCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910