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The map label for this gene is trmH [C]

Identifier: 38234645

GI number: 38234645

Start: 2147125

End: 2147643

Strand: Reverse

Name: trmH [C]

Synonym: DIP2096

Alternate gene names: 38234645

Gene position: 2147643-2147125 (Counterclockwise)

Preceding gene: 38234646

Following gene: 38234644

Centisome position: 86.3

GC content: 58.77

Gene sequence:

>519_bases
GTGGACGCTTACCGTTATTGGTCGCGTGAGGCGATCCGTGCGGACATCGATACGCGTCGCCACTGCTTGCATATTGCGAT
CGAGAACTTCGAAAACGACGCCAACATTGGCACCGTGGTGCGCACCGCTAATGCGTTCGCGGTTGATACCGTGCACATTG
TGGGGCGCAAGCGCTGGAATCGCCGTGGGGCGATGGTGACCGACCGCTACCAGCACCTTATGCACCATCCCACGACTGCC
GACGTGGTGGCGTGGGCTAAGCAACAAGGCCTTACCATCGTTGCGATCGATAACACCCCAGGGTGTGTGCCCTTGGAGAC
TGCCGAGTTGCCTGAGCGTTGCTTGTTGCTGTTTGGCCAAGAAGGCCCTGGAGTAACACAGGAGGCGCAGGATAATGCGC
GGATGACCTGCTCGATTGCCCAGTTTGGTTCGACGCGGTCGATCAATGCGGGTGTAGCCGCGGGTATTGCGATGCATGCG
TGGATCAGACAGCACGCCGATCTTGACAATGCATGGTAG

Upstream 100 bases:

>100_bases
CCACGGGGTAGGGCCGTGGGCTGAGGAGCACCCCCATGAGGAGGCAATTGATCCTCGGTTTGATCCGGAGCTGCTGGAAA
ATGGTGATCGTCGCAACGTC

Downstream 100 bases:

>100_bases
ATTTCACACTCGTGAACGAGATGTGGCTACACCGTGCTGACCTTGCAGAATCTGCAATCAATGAGCGCCATGCGTCGCGG
CTGTGGATGCTACCGCGAAC

Product: putative RNA methylase family protein

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: RNA Methyltransferase; rRNA Methylase; TRNA/RRNA MethyltransferaU; RNA Methyltransferase TrmH Family; tRNA Guanosine-2-O-Methyltransferase; 23S RRNA Methyltransferase; TrmH Family RNA Methyltransferase; Spou RRNA Methylase Family Protein; RNA Methylase Family Protein; Methyltransferase; rRNA MethylaU; SPOU RRNA Methylase Family Protein; TrmH Family TRNA/RRNA Methyltransferase

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MDAYRYWSREAIRADIDTRRHCLHIAIENFENDANIGTVVRTANAFAVDTVHIVGRKRWNRRGAMVTDRYQHLMHHPTTA
DVVAWAKQQGLTIVAIDNTPGCVPLETAELPERCLLLFGQEGPGVTQEAQDNARMTCSIAQFGSTRSINAGVAAGIAMHA
WIRQHADLDNAW

Sequences:

>Translated_172_residues
MDAYRYWSREAIRADIDTRRHCLHIAIENFENDANIGTVVRTANAFAVDTVHIVGRKRWNRRGAMVTDRYQHLMHHPTTA
DVVAWAKQQGLTIVAIDNTPGCVPLETAELPERCLLLFGQEGPGVTQEAQDNARMTCSIAQFGSTRSINAGVAAGIAMHA
WIRQHADLDNAW
>Mature_172_residues
MDAYRYWSREAIRADIDTRRHCLHIAIENFENDANIGTVVRTANAFAVDTVHIVGRKRWNRRGAMVTDRYQHLMHHPTTA
DVVAWAKQQGLTIVAIDNTPGCVPLETAELPERCLLLFGQEGPGVTQEAQDNARMTCSIAQFGSTRSINAGVAAGIAMHA
WIRQHADLDNAW

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790083, Length=149, Percent_Identity=29.5302013422819, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 19191; Mature: 19191

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAYRYWSREAIRADIDTRRHCLHIAIENFENDANIGTVVRTANAFAVDTVHIVGRKRWN
CCCHHHHHHHHHHHCHHHHHHEEEEEEECCCCCCCCEEEEEECCCEEEEEEEEECCCCCC
RRGAMVTDRYQHLMHHPTTADVVAWAKQQGLTIVAIDNTPGCVPLETAELPERCLLLFGQ
CCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCEECCHHHCCHHEEEEECC
EGPGVTQEAQDNARMTCSIAQFGSTRSINAGVAAGIAMHAWIRQHADLDNAW
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDAYRYWSREAIRADIDTRRHCLHIAIENFENDANIGTVVRTANAFAVDTVHIVGRKRWN
CCCHHHHHHHHHHHCHHHHHHEEEEEEECCCCCCCCEEEEEECCCEEEEEEEEECCCCCC
RRGAMVTDRYQHLMHHPTTADVVAWAKQQGLTIVAIDNTPGCVPLETAELPERCLLLFGQ
CCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCEECCHHHCCHHEEEEECC
EGPGVTQEAQDNARMTCSIAQFGSTRSINAGVAAGIAMHAWIRQHADLDNAW
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA