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The map label for this gene is purT [H]

Identifier: 38234614

GI number: 38234614

Start: 2106678

End: 2107574

Strand: Reverse

Name: purT [H]

Synonym: DIP2057

Alternate gene names: 38234614

Gene position: 2107574-2106678 (Counterclockwise)

Preceding gene: 38234617

Following gene: 38234612

Centisome position: 84.69

GC content: 56.63

Gene sequence:

>897_bases
ATGTTTACTCCTGAGCTCATCGCAGATAATGCCACTAAAGTTTCTCTGGTTGGCGACCCTGATTTGGTACGGGAACTGCG
GTTTTCTTTTGAACGTTTGGGCATTGTTGTGACTGATGTTGATCCGGATGGTTCGATTTCGGTGGGGGTGTCGCGTTGGT
CACGTGCGGCGTTGTTGCAGAAGGCTTCGCGGGATTTGGGGCTTCCGGTGCCGCGTTATGCGGTGGCGTCTAATCCGGAT
GAGTTGGAGGTTGCTGCGGCTCAGTTGGGTTTCCCTTGTGTGGTGAAGGGGGATTCGGTTGAGGTGGTGCGTTCGCCGGA
GGATATGCAGTGGCGTGGCGAGTGCGTGGTGGAGAATTTTGTTGATTTTGATCGCAAGATCAGCATGGTTGCGGTGCGTT
CTGTGGATCCTGCGACGGGGGAGTTGGCTACGTGGTATTGCGAGCCGATTAGTGAGGGTATGCAGCCGTTTCCGTTGGGT
GCTGCGACGTTGGATAATGCGCGGAGTGTGGCTGCGCGTATTACGAATGCGATTGGTTGCCGTGGCTTGTTTGTGGTGGA
GATGTTTGTCAAGGGGGATGACGTGTATTTCTCTGATGTGACGATTGGGCCGTCGTTGTCGGGGTTTGTTACGTGTGCTA
CGCAGCGGTTTGGGCAGTTTGATTTGCATGCGCGTGCGCTGTTGGGTTTGCCTATCGACGTCACCCTGACCAGTCCTGGC
GCGATGTCGTCGGTGGGGGTTGATGAGGTTCCTCGTGCGGGTCTTGCGCGTGCGTTGGCTATGTCGGAGGTTAATGTGGT
GGTGGGGCAGACATTGGCGGTGACTCTTGCTACGGCTGATACCGCTGAGCAGGCGCGGGAGTCGGCTCGCGCAGCGGTAT
CTGAGCTGAGGGGTTAG

Upstream 100 bases:

>100_bases
AATGCCGAATCCTTCCCAGGTCAGGATTTCCTTTGTTTCAAGATCTGCTGAATCTGCGTGGTAAGCCATAGTCGAACTTT
AGCGCATGTAGGCTAAAGAA

Downstream 100 bases:

>100_bases
CGTTTCATGGGTTAGTCCTTTTTGGCGGCGTGCTGGAGCATGTCGTTCCATTCGACGATTTTTTTGCGTTCGCGTCCTTC
TGCCTGCCCGAGTTCGCGTT

Product: putative phosphoribosylglycinamide formyltransferase

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MFTPELIADNATKVSLVGDPDLVRELRFSFERLGIVVTDVDPDGSISVGVSRWSRAALLQKASRDLGLPVPRYAVASNPD
ELEVAAAQLGFPCVVKGDSVEVVRSPEDMQWRGECVVENFVDFDRKISMVAVRSVDPATGELATWYCEPISEGMQPFPLG
AATLDNARSVAARITNAIGCRGLFVVEMFVKGDDVYFSDVTIGPSLSGFVTCATQRFGQFDLHARALLGLPIDVTLTSPG
AMSSVGVDEVPRAGLARALAMSEVNVVVGQTLAVTLATADTAEQARESARAAVSELRG

Sequences:

>Translated_298_residues
MFTPELIADNATKVSLVGDPDLVRELRFSFERLGIVVTDVDPDGSISVGVSRWSRAALLQKASRDLGLPVPRYAVASNPD
ELEVAAAQLGFPCVVKGDSVEVVRSPEDMQWRGECVVENFVDFDRKISMVAVRSVDPATGELATWYCEPISEGMQPFPLG
AATLDNARSVAARITNAIGCRGLFVVEMFVKGDDVYFSDVTIGPSLSGFVTCATQRFGQFDLHARALLGLPIDVTLTSPG
AMSSVGVDEVPRAGLARALAMSEVNVVVGQTLAVTLATADTAEQARESARAAVSELRG
>Mature_298_residues
MFTPELIADNATKVSLVGDPDLVRELRFSFERLGIVVTDVDPDGSISVGVSRWSRAALLQKASRDLGLPVPRYAVASNPD
ELEVAAAQLGFPCVVKGDSVEVVRSPEDMQWRGECVVENFVDFDRKISMVAVRSVDPATGELATWYCEPISEGMQPFPLG
AATLDNARSVAARITNAIGCRGLFVVEMFVKGDDVYFSDVTIGPSLSGFVTCATQRFGQFDLHARALLGLPIDVTLTSPG
AMSSVGVDEVPRAGLARALAMSEVNVVVGQTLAVTLATADTAEQARESARAAVSELRG

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=285, Percent_Identity=29.8245614035088, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 31723; Mature: 31723

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTPELIADNATKVSLVGDPDLVRELRFSFERLGIVVTDVDPDGSISVGVSRWSRAALLQ
CCCCHHCCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCEEECHHHHHHHHHHH
KASRDLGLPVPRYAVASNPDELEVAAAQLGFPCVVKGDSVEVVRSPEDMQWRGECVVENF
HHHHHCCCCCCCHHHCCCCCHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCHHHHHHH
VDFDRKISMVAVRSVDPATGELATWYCEPISEGMQPFPLGAATLDNARSVAARITNAIGC
HHHCCCEEEEEEECCCCCCCCEEEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
RGLFVVEMFVKGDDVYFSDVTIGPSLSGFVTCATQRFGQFDLHARALLGLPIDVTLTSPG
CCEEEEEEEECCCEEEEEEEEECCCCCHHEEEHHHHCCCCCHHHHHHCCCCEEEEEECCC
AMSSVGVDEVPRAGLARALAMSEVNVVVGQTLAVTLATADTAEQARESARAAVSELRG
CCCCCCHHHCCHHHHHHHHHHHHHHEEECCEEEEEEEECCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFTPELIADNATKVSLVGDPDLVRELRFSFERLGIVVTDVDPDGSISVGVSRWSRAALLQ
CCCCHHCCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCEEECHHHHHHHHHHH
KASRDLGLPVPRYAVASNPDELEVAAAQLGFPCVVKGDSVEVVRSPEDMQWRGECVVENF
HHHHHCCCCCCCHHHCCCCCHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCHHHHHHH
VDFDRKISMVAVRSVDPATGELATWYCEPISEGMQPFPLGAATLDNARSVAARITNAIGC
HHHCCCEEEEEEECCCCCCCCEEEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
RGLFVVEMFVKGDDVYFSDVTIGPSLSGFVTCATQRFGQFDLHARALLGLPIDVTLTSPG
CCEEEEEEEECCCEEEEEEEEECCCCCHHEEEHHHHCCCCCHHHHHHCCCCEEEEEECCC
AMSSVGVDEVPRAGLARALAMSEVNVVVGQTLAVTLATADTAEQARESARAAVSELRG
CCCCCCHHHCCHHHHHHHHHHHHHHEEECCEEEEEEEECCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]