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The map label for this gene is def [H]

Identifier: 38234602

GI number: 38234602

Start: 2092244

End: 2092807

Strand: Direct

Name: def [H]

Synonym: DIP2045

Alternate gene names: 38234602

Gene position: 2092244-2092807 (Clockwise)

Preceding gene: 38234598

Following gene: 38234603

Centisome position: 84.07

GC content: 59.75

Gene sequence:

>564_bases
ATGATTCGTCCGATCGTTATTTGTGGAGATCCCGTCCTCCATAACCCTACTGCCCCCGTGAGCGACCCAGCAGAAGTCCA
AGACCTCATTGCAGACATGTATGAAACCATGGAAGCAGCTCGTGGTGTGGGTCTTGCCGCTAACCAAGTTGGTGTTGGAT
TGCGCCTTTTCGTGTTCAACTGCCCTGACGACGAGGGCCACTTCCACCGCGGTTGCGTGATCAACCCCGTGCTGGAAACC
TCCGAAATCCCTGAAACCATGCCTGCCGACGACGGCAGCGACGACGAAGGCTGCCTCTCCGTCCCAGGCGAAGGCTTCCC
CACCGGTCGTGCCGACTGGGCACGCGTGACCGGCCTTGAAGCCGACGGCACCCCCGTGGTCTACGAGGGCACTGGATTCC
TCGCCCGCTGCTTCCAGCACGAAGTGGGCCACCTCGACGGCTTCCTCTACACCGACACTTTGATCGGCCGGTGGAAGCGC
CAAGCTAAAAAAGCCATTAAACGCAACGGTTGGTCTGCGGGTATGACTTGGATGCCGGGGGTGGACCCTGATCCGTTCCA
CTAA

Upstream 100 bases:

>100_bases
CACCGGCGTAAGCCCAAGTTCACGGATGGCTTCCTCCTTGGCACCGAGGCTGCGCGGGGCTTTTTCTTCGAATGCGAGCA
AGTCAAGATAATGTATAGGC

Downstream 100 bases:

>100_bases
ATTCCGCACCGGTCCGGTGCAGGTCGGCGACCGCATTGTGGTCCGCCAAGAGGAATTCGACACCATTGGGCACGTTTTAA
GCGTGGATCCGCTGATCGTG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MIRPIVICGDPVLHNPTAPVSDPAEVQDLIADMYETMEAARGVGLAANQVGVGLRLFVFNCPDDEGHFHRGCVINPVLET
SEIPETMPADDGSDDEGCLSVPGEGFPTGRADWARVTGLEADGTPVVYEGTGFLARCFQHEVGHLDGFLYTDTLIGRWKR
QAKKAIKRNGWSAGMTWMPGVDPDPFH

Sequences:

>Translated_187_residues
MIRPIVICGDPVLHNPTAPVSDPAEVQDLIADMYETMEAARGVGLAANQVGVGLRLFVFNCPDDEGHFHRGCVINPVLET
SEIPETMPADDGSDDEGCLSVPGEGFPTGRADWARVTGLEADGTPVVYEGTGFLARCFQHEVGHLDGFLYTDTLIGRWKR
QAKKAIKRNGWSAGMTWMPGVDPDPFH
>Mature_187_residues
MIRPIVICGDPVLHNPTAPVSDPAEVQDLIADMYETMEAARGVGLAANQVGVGLRLFVFNCPDDEGHFHRGCVINPVLET
SEIPETMPADDGSDDEGCLSVPGEGFPTGRADWARVTGLEADGTPVVYEGTGFLARCFQHEVGHLDGFLYTDTLIGRWKR
QAKKAIKRNGWSAGMTWMPGVDPDPFH

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=161, Percent_Identity=34.7826086956522, Blast_Score=94, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24645728, Length=170, Percent_Identity=30.5882352941176, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20279; Mature: 20279

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRPIVICGDPVLHNPTAPVSDPAEVQDLIADMYETMEAARGVGLAANQVGVGLRLFVFN
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEE
CPDDEGHFHRGCVINPVLETSEIPETMPADDGSDDEGCLSVPGEGFPTGRADWARVTGLE
CCCCCCCEECCCEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHEECCC
ADGTPVVYEGTGFLARCFQHEVGHLDGFLYTDTLIGRWKRQAKKAIKRNGWSAGMTWMPG
CCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
VDPDPFH
CCCCCCC
>Mature Secondary Structure
MIRPIVICGDPVLHNPTAPVSDPAEVQDLIADMYETMEAARGVGLAANQVGVGLRLFVFN
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEE
CPDDEGHFHRGCVINPVLETSEIPETMPADDGSDDEGCLSVPGEGFPTGRADWARVTGLE
CCCCCCCEECCCEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHEECCC
ADGTPVVYEGTGFLARCFQHEVGHLDGFLYTDTLIGRWKRQAKKAIKRNGWSAGMTWMPG
CCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
VDPDPFH
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]