LOCUS NC_002252 7258 bp DNA circular BCT 03-MAR-2010 DEFINITION Buchnera aphidicola str. APS (Acyrthosiphon pisum) plasmid pTrp, complete sequence. ACCESSION NC_002252 VERSION NC_002252.1 GI:10957099 DBLINK Project:245 KEYWORDS . SOURCE Buchnera aphidicola str. APS (Acyrthosiphon pisum) ORGANISM Buchnera aphidicola str. APS (Acyrthosiphon pisum) Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Buchnera. REFERENCE 1 (sites) AUTHORS Shigenobu,S., Watanabe,H., Hattori,M., Sakaki,Y. and Ishikawa,H. TITLE Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS JOURNAL Nature 407 (6800), 81-86 (2000) PUBMED 10993077 REFERENCE 2 (bases 1 to 7258) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (24-OCT-2000) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 7258) CONSRTM NCBI Microbial Genomes Annotation Project TITLE Direct Submission JOURNAL Submitted (17-JAN-2000) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AP001070. COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..7258 /organism="Buchnera aphidicola str. APS (Acyrthosiphon pisum)" /mol_type="genomic DNA" /strain="APS" /isolate="Tokyo1998" /host="Acyrthosiphon pisum" /db_xref="taxon:107806" /plasmid="pTrp" /note="primary endosymbiont of Acyrthosiphon pisum; trpEG plasmid" gene 1..1566 /gene="trpE" /locus_tag="BUpT01" /db_xref="GeneID:1109444" CDS 1..1566 /gene="trpE" /locus_tag="BUpT01" /EC_number="4.1.3.27" /note="with component II, the glutamine amidotransferase, catalyzes the formation of anthranilate from chorismate and glutamine" /codon_start=1 /transl_table=11 /product="anthranilate synthase component I" /protein_id="NP_057962.1" /db_xref="GI:10957100" /db_xref="GeneID:1109444" /translation="MFLIEKRRKLIQKKANYHSDPTTVFNHLCGSRPATLLLETAEVN KKNNLESIMIVDSAIRVSAVKNSVKITALSENGAEILSILKENPHKKIKFFEKNKSIN LIFPSLDNNLDEDKKIFSLSVFDSFRFIMKSVNNTKRTSKAMFFGGLFSYDLISNFES LPNVKKKQKCPDFCFYLAETLLVVDHQKKTCLIQSSLFGRNVDEKNRIKKRTEEIEKK LEEKLTSIPKNKTTVPVQLTSNISDFQYSSTIKKLQKLIQKGEIFQVVPSRKFFLPCD NSLSAYQELKKSNPSPYMFFMQDEDFILFGASPESSLKYDEKNRQIELYPIAGTRPRG RKKDGTLDLDLDSRIELEMRTNHKELAEHLMLVDLARNDLARICEPGSRYVSDLVKVD KYSHVMHLVSKVVGQLKYGLDALHAYSSCMNMGTLTGAPKVRAMQLIAEYEGEGRGSY GGAIGYFTDLGNLDTCITIRSAYVESGVATIQAGAGVVFNSIPEDEVKESLNKAQAVI NAIKKAHFTMGSS" variation 256 /gene="trpE" /locus_tag="BUpT01" /note="polymorphism: G or A" variation 1215 /gene="trpE" /locus_tag="BUpT01" /note="polymorphism: G or C" variation 1512 /gene="trpE" /locus_tag="BUpT01" /note="polymorphism: G or A" gene 1569..2171 /gene="trpG" /locus_tag="BUpT02" /db_xref="GeneID:1109446" CDS 1569..2171 /gene="trpG" /locus_tag="BUpT02" /EC_number="4.1.3.27" /note="TrpG; with TrpE catalyzes the formation of anthranilate and glutamate from chorismate and glutamine; TrpG provides the glutamine amidotransferase activity" /codon_start=1 /transl_table=11 /product="anthranilate synthase component II" /protein_id="NP_057963.1" /db_xref="GI:10957101" /db_xref="GeneID:1109446" /translation="MANILLLDNIDSFTYNLVEQLRNQKNNVLVYRNTVSIDIIFNSL KKLTHPILMLSPGPSLPKHAGCMLDLIKKVKGDIPIVGICLGHQAIVEAYGGIIGYAG EIFHGKASLIRHDGLEMFEGVPQPLPVARYHSLICNKIPEKFVINSYFEKMIMSVRNN CDRVCGFQFHPESILTTHGDQILEKIIHWASLKYITNKKQ" variation 2170 /gene="trpG" /locus_tag="BUpT02" /note="polymorphism: A or none" gene 3629..5196 /gene="trpE2" /locus_tag="BUpT03" /db_xref="GeneID:1109445" misc_feature 3629..5196 /gene="trpE2" /locus_tag="BUpT03" /note="authentic frameshift; This 1568 nt putative ORF is 99 pct identical to trpE" gene 5199..5801 /gene="trpG2" /locus_tag="BUpT04" /db_xref="GeneID:1109447" CDS 5199..5801 /gene="trpG2" /locus_tag="BUpT04" /EC_number="4.1.3.27" /note="TrpG; with TrpE catalyzes the formation of anthranilate and glutamate from chorismate and glutamine; TrpG provides the glutamine amidotransferase activity" /codon_start=1 /transl_table=11 /product="anthranilate synthase component II" /protein_id="NP_057964.1" /db_xref="GI:10957102" /db_xref="GeneID:1109447" /translation="MANILLLDNIDSFTYNLVEQLRNQKNNVLVYRNTVSIDIIFNSL KKLTHPILMLSPGPSLPKHAGCMLDLIKKVKGDIPIVGICLGHQAIVEAYGGIIGYAG EIFHGKASLIRHDGLEMFEGVPQPLPVARYHSLICNKIPEKFVINSYFEKMIMSVRNN CDRVCGFQFHPESILTTHGDQILEKIIHWASLKYITNKKQ" variation 6041 /note="polymorphism: T or C" variation 6242 /note="polymorphism: A or T" variation 6993 /note="polymorphism: G or A" variation 7149 /note="polymorphism: A or G" ORIGIN 1 atttttttga ttgaaaagag acgtaaatta atacaaaaaa aggctaatta tcactctgat 61 cctacaacag tttttaatca tctttgtgga tcaagacccg caacattatt actggaaaca 121 gcagaagtta ataaaaaaaa taatttagaa agtataatga ttgtcgatag cgcgatacgc 181 gtctctgctg tcaagaattc agttaagatt actgcgctct ctgaaaacgg agcagagata 241 ttatctattt taaaagaaaa ccctcataaa aaaataaaat tttttgaaaa aaacaaaagt 301 atcaacttga tctttccctc tttagacaac aatcttgatg aagataaaaa aattttttct 361 ttatcagtat ttgattcttt cagattcatt atgaaatctg ttaacaacac aaaaagaaca 421 agtaaagcaa tgttttttgg cggattgttt tcttatgatc ttatttctaa ttttgaatcg 481 ttgccgaatg taaagaaaaa acaaaaatgc ccggattttt gtttttattt agcagaaaca 541 ttacttgttg tagatcatca aaaaaaaaca tgtttgattc aaagcagttt atttggcaga 601 aatgtagatg aaaaaaatag aattaaaaaa agaacagaag aaatagaaaa gaaactggaa 661 gaaaaattga catccattcc taaaaataag acaactgtac cagttcaatt gacttccaat 721 ataagtgatt ttcaatattc atctacaata aaaaaattac aaaaattaat tcaaaaaggt 781 gaaattttcc aagtcgtgcc ctctcgaaag ttttttttgc cttgtgacaa ttcattatca 841 gcatatcaag aattaaaaaa aagcaatccc agtccctata tgttttttat gcaagatgaa 901 gatttcatac tattcggtgc atctccagaa agttctttaa aatatgatga aaaaaataga 961 caaattgagc tctatcctat tgctggaacc agacccagag gtagaaaaaa agacggaact 1021 ttagatctcg atttagacag cagaatagaa cttgaaatga gaactaatca taaagaactt 1081 gctgaacatt tgatgctggt tgacttagca agaaatgatc ttgcacgtat ttgtgagccg 1141 ggatctagat atgtttccga tctagttaag gtggataaat attcgcacgt gatgcatttg 1201 gtttctaaag tagtggggca attaaaatat ggtttagatg cgcttcacgc atattcctct 1261 tgtatgaata tggggacatt aactggtgca cccaaagttc gagcgatgca attaattgct 1321 gaatatgaag gggaagggag aggaagttac ggcggcgcta taggttattt tactgattta 1381 ggaaatttag atacttgcat cacaatacgt tcagcttatg tggaatcagg tgtcgcaaca 1441 attcaagcag gagccggtgt tgtttttaat tcaatacctg aagacgaagt gaaagagagt 1501 ttaaacaagg cgcaagctgt aataaatgct ataaaaaaag cacattttac aatgggatcc 1561 tcttaagaat ggcaaatatt ttactgttag acaacataga ctcattcacc tataatttag 1621 ttgaacaact gagaaatcaa aaaaacaatg ttttagttta tcgtaataca gtgagtattg 1681 atattatttt caattccctt aaaaaattaa cacatccgat tttaatgctg tcgcccggac 1741 ctagtttgcc gaaacacgca ggatgcatgt tagatttaat aaaaaaagtc aagggggaca 1801 ttcctatagt aggaatttgt ttaggtcatc aagcaatagt agaagcgtat ggcggcatta 1861 tcggatatgc aggtgaaata ttccatggca aagcatcatt aatccgccat gatggtttag 1921 agatgtttga gggtgtaccg caaccactac ctgttgctcg ataccattcc ttaatttgca 1981 acaaaattcc ggagaaattt gtcattaatt cctattttga aaaaatgatt atgtctgtaa 2041 gaaacaattg tgatcgtgtt tgcggctttc aatttcatcc tgaatctatt ttaacaacac 2101 atggagacca aatattagaa aaaattattc attgggcgtc tttaaaatat atcacaaaca 2161 agaaacaata aaaaaaaaga ttctactcaa acgagtcgtt tttttaaatt ccatatccct 2221 ttttataaca ctgaatcctc ctgcgtcatc gcattacaca attcaacatc cagacgtgcc 2281 tgaaaacaaa aacatacaac aatgattctc atgcaattgg aatgacaaaa tcaaatatac 2341 acaatgaaaa ttttttaata aaacccgtac cgaaattatt gaaaagaagt tttaaaacag 2401 tgttaatgtt cccaaaaaca ggaggaaaaa tcacaaaaaa tacattagaa aaaatagaaa 2461 gtatgaaaaa gagaaaatta ttcaaggaaa ggtgggtgta atttttactt catacaaacc 2521 acaacaagaa ttgattaaaa ctggtcatcg aggtgaatac agatatcaaa ttaaatgaaa 2581 gaattggtcg aaatctgtga ctgtcaatac tggaatgtat ctcagaaaat aagtcaaact 2641 ttatcacgat gggtattttt tcattaatag agattatcaa accgacataa aaaaaggggg 2701 agcgatttta agtactatgt cacttttgta tgcatgagtc taaatgactc gactttaaat 2761 cggtcttgtc agcctcataa gcacgctgta tgtgtgtaaa tttgtgctcc attagaaaga 2821 aaatacattt ggtaaagggg aggagaagac tagcggtgac tgtcggatcc aagagcatca 2881 tcaatcttct tatagatcgc tcttctatca gttttctaaa aagcagttct caaaatttaa 2941 gtgaactccc tgtctgaaaa atatatgagt cccggaaaac gcacttcaat caccaataaa 3001 agataattca tgattctgag acagatgaaa acaaaagttg gacaaacaat gatagagata 3061 aaatatctcc atataaaaat tttataaaat gaactgttgt cagtcataca aaacaattca 3121 ataaaaagga aaaaatgaaa atttttttct aaaaagttat ccacagtttc tgtctacaag 3181 tgaattttaa aaaatatgaa attttaagat aaattccaaa acaaaatact ctaacattat 3241 gttttttata aaaaaatttt ttttttaatt catacaatac atgttgataa catgttcata 3301 agtctgggga taagttttgt atatatatac aagacacttt ccgatacata ggaatcttac 3361 gcggaaaaaa gaaaaacatt aaaaacaata aattaattaa gattagcatt ctttaaaaat 3421 ccttttaaaa gaaaaaaaca aatacatgta ttatatataa ggtttttaat tgttattgtt 3481 gttttgtatt atactattaa ttagtatcac tttatttaac atatttatta aatacttaaa 3541 tatatattat acattttttg taaaatcaaa aatgatatta ttgctttaat ttaaaaagtt 3601 agttattata aaacgaaaat tagatgaaat ttttttgatt gaaaagagac gtaaattaat 3661 acaaaaaaag gctaattatc actctgatcc tacaacagtt tttaatcatc tttgtggatc 3721 aagacccgca acattattac tggaaacagc agaagttaat aaaaaaaata atttagaaag 3781 tataatgatt gtcgatagcg cgatacgcgt ctctgctgtc aagaattcag ttaagattac 3841 tgcgctctct gaaaacggag cagagatatt atctatttta aaagaaaacc ctcataaaaa 3901 aataaaattt tttgaaaaaa acaaaagtat caacttgatc tttccctctt tagacaacaa 3961 tcttgatgaa gataaaaaaa ttttttcttt atcagtattt gattctttca gattcattat 4021 gaaatctgtt aacaacacaa aaagaacaag taaagcaatg ttttttggcg gattgttttc 4081 ttatgatctt atttctaatt ttgaatcgtt gccgaatgta aagaaaaaac aaaaatgccc 4141 ggatttttgt ttttatttag cagaaacatt acttgttgta gatcatcaaa aaaaaacatg 4201 tttgattcaa agcagtttat ttggcagaaa tgtagatgaa aaaaatagaa ttaaaaaaag 4261 aacagaagaa atagaaaaga aactggaaga aaaattgaca tccattccta aaaataagac 4321 aactgtacca gttcaattga cttccaatat aagtgatttt caatattcat ctacaataaa 4381 aaaattacaa aaattaattc aaaaaggtga aattttccaa gtcgtgccct ctcgaaagtt 4441 ttttttgcct tgtgacaatt cattatcagc atatcaagaa ttaaaaaaaa gcaatcccag 4501 tccctatgtg ttttttatgc aagatgaaga tttcatacta ttcggtgcat ctccagaaag 4561 ttctttaaaa tatgatgaaa aaaatagaca aattgagctc tatcctattg ctggaaccag 4621 acccagaggt agaaaaaaag acggaacttt agatctcgat ttagacagca gaatagaact 4681 tgaaatgaga actaatcata aagaacttgc tgaacatttg atgctggttg acttagcaag 4741 aaatgatctt gcacgtattt gtgagccggg atctagatat gtttccgatc tagttaaggt 4801 ggataaatat tcgcacgtga tgcatttggt ttctaaagta gtggggcaat taaaatatgg 4861 tttagatgcg cttcacgcat attcctcttg tatgaatatg gggacattaa ctggtgcacc 4921 caaagttcga gcgatgcaat taattgctga atatgaaggg gaagggagag gaagttacgg 4981 cggcgctata ggttatttta ctgatttagg aaatttagat acttgcatca caatacgttc 5041 agcttatgtg gaatcaggtg tcgcaacaat tcaagcagga gccggtgttg tttttaattc 5101 acaatacctg aagacgaagt gaaagagagt ttaaacaagg cgcaagctgt aataaatgct 5161 ataaaaaaag cacattttac aatgggatcc tcttaagaat ggcaaatatt ttactgttag 5221 acaacataga ctcattcacc tataatttag ttgaacaact gagaaatcaa aaaaacaatg 5281 ttttagttta tcgtaataca gtgagtattg atattatttt caattccctt aaaaaattaa 5341 cacatccgat tttaatgctg tcgcccggac ctagtttgcc gaaacacgca ggatgcatgt 5401 tagatttaat aaaaaaagtc aagggggaca ttcctatagt aggaatttgt ttaggtcatc 5461 aagcaatagt agaagcgtat ggcggcatta tcggatatgc aggtgaaata ttccatggca 5521 aagcatcatt aatccgccat gatggtttag agatgtttga gggtgtaccg caaccactac 5581 ctgttgctcg ataccattcc ttaatttgca acaaaattcc ggagaaattt gtcattaatt 5641 cctattttga aaaaatgatt atgtctgtaa gaaacaattg tgatcgtgtt tgcggctttc 5701 aatttcatcc tgaatctatt ttaacaacac atggagacca aatattagaa aaaattattc 5761 attgggcgtc tttaaaatat atcacaaaca agaaacaata aaaaaaaaag attctactca 5821 aacgagtcgt ttttttaaat tccatatccc tttttataac actgaatcct cctgcgtcat 5881 cgcattacac aattcaacat ccagacgtgc ctgaaaacaa aaacatacaa caatgattct 5941 catgcaattg gaatgacaaa atcaaatata cacaatgaaa attttttaat aaaaccgtac 6001 ccgaaattat tgaaaagaag ttttaaaaca gtgttaatgt tcccaaaaac aggaggaaaa 6061 atcacaaaaa atacattaga aaaaatagaa agtatgaaaa agagaaaatt attcaaggaa 6121 aggtgggtgt aatttttact tcatacaaac cacaacaaga attgattaaa actggtcatc 6181 gaggtgaata cagatatcaa attaaatgaa agaattggtc gaaatctgtg actgtcaata 6241 caggaatgta tctcagaaaa taagtcaaac tttatcacga tgggtatttt ttcattaata 6301 gagattatca aaccgacata aaaaaagggg ggcgatttta agtactatgt cacttttgta 6361 tgcatgagtc taaatgactc gactttaaat cggtcttgtc agcctcataa gcacgctgta 6421 tgtgtgtaaa tttgtgctcc attagaaaga aaatacattt ggtaaagggg aggagaagac 6481 tagcggtgac tgtcggatcc aagagcatca tcaatcttct tatagatcgc tcttctatca 6541 gttttctaaa aagcagttct caaaatttaa gtgaactccc tgtctgaaaa atatatgagt 6601 cccggaaaac gcacttcaat caccaataaa agataattca tgattctgag acagatgaaa 6661 acaaaagttg gacaaacaat gatagagata aaatatctcc atataaaaat tttataaaat 6721 gaactgttgt cagtcataca aaacaattca ataaaaagga aaaaaatgaa aatttttttc 6781 taaaaagtta tccacagttt ctgtctacaa gtgaatttta aaaaatatga aattttaaga 6841 taaattccaa aacaaaatac tctaacatta tgttttttat aaaaaaattt ttttttaatt 6901 catacaatac atgttgataa catgttcata agtctgggga taagttttgt atatatatac 6961 aagacacttt ccgatacata ggaatcttac gcggaaaaaa gaaaaacatt aaaaacaata 7021 aattaattaa gattagcatt ctttaaaaat ccttttaaaa gaaaaaaaca aatacatgta 7081 ttatatataa ggtttttaat tgttattgtt gttttgtatt atactattaa ttagtatcac 7141 tttatttaac atatttatta aatacttaaa tatatattat acattttttg taaaatcaaa 7201 aatgatatta ttgctttaat ttaaaaagtt agttattata aaacgaaaat tagatgaa //