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The map label for this gene is nikA [H]

Identifier: 222102875

GI number: 222102875

Start: 553410

End: 554990

Strand: Direct

Name: nikA [H]

Synonym: Avi_7613

Alternate gene names: 222102875

Gene position: 553410-554990 (Clockwise)

Preceding gene: 222102873

Following gene: 222102876

Centisome position: 87.6

GC content: 53.83

Gene sequence:

>1581_bases
ATGATCAAAACTTGCTTTCGCCATGCGGCACTGGCGCTGACGCTTGCCTTTTGCAGCCCTCTTACCAATGCGCAGGCGGG
CGAGACACTGAATTTTTCATGGCCCATGAATGTCGGCCCGCTCAATCCCCATCTCTATTCACCCAATCAGATGTTTGCGC
AAAACATGGTGTATGAACCGCTGGTGCGTTATCAGGCCGATGGCACCGTCAAGCCATGGCTGGCGCAATCGTGGGAGATT
TCGAACGAGGGGCGGACCTATACGTTTCAATTGCGCGACGGTGTGCGCTTTTCAAACGGCGAACCCTTTGATGCAGCAGC
GGTAAAAGCCAATATTGATGCGGTGCTGGCCAATCGCTCCCGTCATGCATGGTTGGAATTGGCCAATGAGATCGAAAGCG
CTGATGTCGTTGACTCTTCGCATGTGCGCATCACACTGAAACACGCCTATTATCCTTTGCTTCAGGAATTGTCCCTGCCC
CGGCCCTTTCGGTTTATCGCCCCTTCGCAGTTCAGGCAGGGCGGCACAAAGGATGGCATTGCCCAGCCGATTGGCACTGG
CCCATGGGTTTTGCAAGAAACCCGCTTGGGCGAAAAAGACGTGTTTGTCCGCAACGACCGCTATTGGGGTGGCAAACCTG
CCTTCGACAGTGTGACCGTCAAGGTTATTCCCGATCCCAACAGCCGCGCCATTGCGTTTGAGACCGGCGATATTGATCTG
ATCTATGGGGCCGATGGGCCGATCTCTCCCGATACGTTTGAGCGTTTCCGCAAAATGGGAACTTATACAACAGAGCTTTC
GCAGCCTTTGGAAACCTTGGTGCTGGCCATCAATACGAACCTTGGTGCCACAAAGGAACTTGCTGTGCGCAAGGCCATCA
ACCATGCGGTGGACAAGGACAAGATGATTGCCACCGTGCTCTATGGCACCCAGCAACGCGCTGACACTCTTTTTGCCCCC
AATGTTCCCTATGCCAATATTGGTCTCAAACCCTACCAGTTTGATCGATCCGTTGCGGAAAAGCTGCTGGATGACGCTGG
CTGGAAACGCCTTGATCCGAAAGGCATTCGGCAGAAGGATGGCAAGCCGCTGTCCATCGAATTATGCTTTATCGGCACAG
ACGCCATCAGCAAATCCATGGCCGAGATTGTGCAGGCTGATCTTGGTAAGGTGGGAATTCAGGTTATCCTGACCGGAGAG
GAAGAAAGCAGCATTTTGTCGCGTCAGCGCGATGGCCGGTTTGGCATGATTTTCAACCGCACCTGGGGGCCTCCTTATGA
TCCTCACGCCTTTGTCAGTTCCATGCGCATTCCCTCCCATGCCGATTATCAGGCGCAGCTTGGCCTGCCGGACAAGGCGA
AGATTGATCAGGAAATCGGCGACGTGCTGATCTCCACGGATGAACACATCCGGCAGGATCTGTATCGGGATATTCTGACC
CGATTGCATGATGAAGCCGTCTATCTGCCGCTCACCTATGTTACCGCCATTGCCGTTGCCAAAAAGGAGCTTGGCCCCAT
TCCGTTTGGGGCGATGTCGAGCGAAATTCCGTTTGATCAGATCAAGCAAAAGACGAACTGA

Upstream 100 bases:

>100_bases
CCGCTTCCAGCCTTTGTCCCGATAGACGGTTGACCTGACAATGACAGTATGATGTATCAGAAAAAACATCTTACTTCATG
ATTGCTCAGCAGGACCTTCC

Downstream 100 bases:

>100_bases
TCCATGGGCGCGTTTATCCTGCGGCGCATTTTGCTGCTGATCCCAATGCTGCTTGGCGCATCACTGGTGATATTTCTCAT
GCTGCGGCTTGGTCCGAGCG

Product: nickel ABC transporter periplasmic nickel-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MIKTCFRHAALALTLAFCSPLTNAQAGETLNFSWPMNVGPLNPHLYSPNQMFAQNMVYEPLVRYQADGTVKPWLAQSWEI
SNEGRTYTFQLRDGVRFSNGEPFDAAAVKANIDAVLANRSRHAWLELANEIESADVVDSSHVRITLKHAYYPLLQELSLP
RPFRFIAPSQFRQGGTKDGIAQPIGTGPWVLQETRLGEKDVFVRNDRYWGGKPAFDSVTVKVIPDPNSRAIAFETGDIDL
IYGADGPISPDTFERFRKMGTYTTELSQPLETLVLAINTNLGATKELAVRKAINHAVDKDKMIATVLYGTQQRADTLFAP
NVPYANIGLKPYQFDRSVAEKLLDDAGWKRLDPKGIRQKDGKPLSIELCFIGTDAISKSMAEIVQADLGKVGIQVILTGE
EESSILSRQRDGRFGMIFNRTWGPPYDPHAFVSSMRIPSHADYQAQLGLPDKAKIDQEIGDVLISTDEHIRQDLYRDILT
RLHDEAVYLPLTYVTAIAVAKKELGPIPFGAMSSEIPFDQIKQKTN

Sequences:

>Translated_526_residues
MIKTCFRHAALALTLAFCSPLTNAQAGETLNFSWPMNVGPLNPHLYSPNQMFAQNMVYEPLVRYQADGTVKPWLAQSWEI
SNEGRTYTFQLRDGVRFSNGEPFDAAAVKANIDAVLANRSRHAWLELANEIESADVVDSSHVRITLKHAYYPLLQELSLP
RPFRFIAPSQFRQGGTKDGIAQPIGTGPWVLQETRLGEKDVFVRNDRYWGGKPAFDSVTVKVIPDPNSRAIAFETGDIDL
IYGADGPISPDTFERFRKMGTYTTELSQPLETLVLAINTNLGATKELAVRKAINHAVDKDKMIATVLYGTQQRADTLFAP
NVPYANIGLKPYQFDRSVAEKLLDDAGWKRLDPKGIRQKDGKPLSIELCFIGTDAISKSMAEIVQADLGKVGIQVILTGE
EESSILSRQRDGRFGMIFNRTWGPPYDPHAFVSSMRIPSHADYQAQLGLPDKAKIDQEIGDVLISTDEHIRQDLYRDILT
RLHDEAVYLPLTYVTAIAVAKKELGPIPFGAMSSEIPFDQIKQKTN
>Mature_526_residues
MIKTCFRHAALALTLAFCSPLTNAQAGETLNFSWPMNVGPLNPHLYSPNQMFAQNMVYEPLVRYQADGTVKPWLAQSWEI
SNEGRTYTFQLRDGVRFSNGEPFDAAAVKANIDAVLANRSRHAWLELANEIESADVVDSSHVRITLKHAYYPLLQELSLP
RPFRFIAPSQFRQGGTKDGIAQPIGTGPWVLQETRLGEKDVFVRNDRYWGGKPAFDSVTVKVIPDPNSRAIAFETGDIDL
IYGADGPISPDTFERFRKMGTYTTELSQPLETLVLAINTNLGATKELAVRKAINHAVDKDKMIATVLYGTQQRADTLFAP
NVPYANIGLKPYQFDRSVAEKLLDDAGWKRLDPKGIRQKDGKPLSIELCFIGTDAISKSMAEIVQADLGKVGIQVILTGE
EESSILSRQRDGRFGMIFNRTWGPPYDPHAFVSSMRIPSHADYQAQLGLPDKAKIDQEIGDVLISTDEHIRQDLYRDILT
RLHDEAVYLPLTYVTAIAVAKKELGPIPFGAMSSEIPFDQIKQKTN

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=519, Percent_Identity=63.1984585741811, Blast_Score=692, Evalue=0.0,
Organism=Escherichia coli, GI1789966, Length=524, Percent_Identity=24.618320610687, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1787052, Length=406, Percent_Identity=25.8620689655172, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787762, Length=524, Percent_Identity=23.8549618320611, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87081878, Length=397, Percent_Identity=24.4332493702771, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787551, Length=475, Percent_Identity=23.1578947368421, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789397, Length=481, Percent_Identity=22.2453222453222, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58657; Mature: 58657

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTCFRHAALALTLAFCSPLTNAQAGETLNFSWPMNVGPLNPHLYSPNQMFAQNMVYEP
CCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEECCHHHHHHHHHHHH
LVRYQADGTVKPWLAQSWEISNEGRTYTFQLRDGVRFSNGEPFDAAAVKANIDAVLANRS
HHHCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCHHEECCCC
RHAWLELANEIESADVVDSSHVRITLKHAYYPLLQELSLPRPFRFIAPSQFRQGGTKDGI
HHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHCCCCCCEEECCCHHHHCCCCCCCC
AQPIGTGPWVLQETRLGEKDVFVRNDRYWGGKPAFDSVTVKVIPDPNSRAIAFETGDIDL
CCCCCCCCCEEECCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCEEE
IYGADGPISPDTFERFRKMGTYTTELSQPLETLVLAINTNLGATKELAVRKAINHAVDKD
EECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCCH
KMIATVLYGTQQRADTLFAPNVPYANIGLKPYQFDRSVAEKLLDDAGWKRLDPKGIRQKD
HEEEEEEECCHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GKPLSIELCFIGTDAISKSMAEIVQADLGKVGIQVILTGEEESSILSRQRDGRFGMIFNR
CCCEEEEEEEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCEEEEEEC
TWGPPYDPHAFVSSMRIPSHADYQAQLGLPDKAKIDQEIGDVLISTDEHIRQDLYRDILT
CCCCCCCHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHEEECCHHHHHHHHHHHHHH
RLHDEAVYLPLTYVTAIAVAKKELGPIPFGAMSSEIPFDQIKQKTN
HHCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MIKTCFRHAALALTLAFCSPLTNAQAGETLNFSWPMNVGPLNPHLYSPNQMFAQNMVYEP
CCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEECCHHHHHHHHHHHH
LVRYQADGTVKPWLAQSWEISNEGRTYTFQLRDGVRFSNGEPFDAAAVKANIDAVLANRS
HHHCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHCCHHEECCCC
RHAWLELANEIESADVVDSSHVRITLKHAYYPLLQELSLPRPFRFIAPSQFRQGGTKDGI
HHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHCCCCCCEEECCCHHHHCCCCCCCC
AQPIGTGPWVLQETRLGEKDVFVRNDRYWGGKPAFDSVTVKVIPDPNSRAIAFETGDIDL
CCCCCCCCCEEECCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCEEE
IYGADGPISPDTFERFRKMGTYTTELSQPLETLVLAINTNLGATKELAVRKAINHAVDKD
EECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCCH
KMIATVLYGTQQRADTLFAPNVPYANIGLKPYQFDRSVAEKLLDDAGWKRLDPKGIRQKD
HEEEEEEECCHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GKPLSIELCFIGTDAISKSMAEIVQADLGKVGIQVILTGEEESSILSRQRDGRFGMIFNR
CCCEEEEEEEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCEEEEEEC
TWGPPYDPHAFVSSMRIPSHADYQAQLGLPDKAKIDQEIGDVLISTDEHIRQDLYRDILT
CCCCCCCHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHEEECCHHHHHHHHHHHHHH
RLHDEAVYLPLTYVTAIAVAKKELGPIPFGAMSSEIPFDQIKQKTN
HHCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]