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The map label for this gene is pstA [H]

Identifier: 158333403

GI number: 158333403

Start: 179281

End: 180216

Strand: Reverse

Name: pstA [H]

Synonym: AM1_0175

Alternate gene names: 158333403

Gene position: 180216-179281 (Counterclockwise)

Preceding gene: 158333404

Following gene: 158333402

Centisome position: 2.77

GC content: 47.01

Gene sequence:

>936_bases
ATGACTTTATCAAATCCCAAGGATTTACCCGGTCTGGAAGACAGTGAAAATTCACAATTTAAGCCTGCCCTTGATACCAG
ATATCGATGGGATCGTTTCTTTGAGGCCCTGACGTGGCTGGCCACCATTATTAGTTTGGTGGTATTGGCGACATTGCTAT
TCGATGTGCTCTCTGACGGTGCTAGCAGTCTGAGTGTTGACTTTTTAACCAGTCCCCCTTCCCGTAAAGCTGCAAAAGCT
GGGCTTTGGCCAGCGCTGATGGGTACTGTTTGGCTCCTCTGCATTACTGCAGCAGTTGCTTTTCCTATCGGTGTGGGTTC
TGGCATCTTTCTAGAAGAGTTTGCCCCCGATAACCTTTTTACTCGGATTATCGAAATTAATGTTGGAAATTTGGCAGCAG
TCCCATCTATTGTCTATGGTCTGTTGGGACTAACGGTTTTTGTGGACATTATGCGTCCAATTACGGGTGGCAGCAGTGTC
CTAGCTGGAGGCTTAACGTTAGCTTTATTAATTTTGCCCATTATTATTGTCAGTACCCGTGAGGCTCTGCGAGCGGTCCC
TAGTAGTTTGCGCCAGGGAGGATTTGCCCTAGGGGCAACGCGCTGGCAAGTTATTCGGGACCTGGTTTTTCCCCAATCTC
TTCCCGGTATTTTGACGGGAACCATTCTGGCCCTGTCTCGTGCCATTGGCGAAACAGCTCCTATTTTAGTAGTGGGTGCT
GCGGGATATATCGCTTTTGCCCCCCCTTTATCTCTTGAAGGGCTGCAATCAGACTTTACTGCACTGCCTATCCAAATTTA
TGTCTGGATTGCCCGAGCTCAGAAATCATTTGAGCCGATTGCAGCTGGAGGCATCATTATTTTGTTAGTGGTCCTGTTGC
TTATGAATACAACCGCTATTCTGCTTCGTAATAAATTCCAGAATCAAAAGTTGTAA

Upstream 100 bases:

>100_bases
ATAAAACCTTGTTTGTCGTTGGGATGACGTTGTTTCTGATCACCTTTTGCCTGAATGTCTTTAGCTTCTGGTTTGTCCGC
CGTTTCCAGGAGAAATACGA

Downstream 100 bases:

>100_bases
CGAACATCATCCTTTGCGGAAAATTTACACATGACCTTATTTCTTAACCGAGTAATAAGCTGTGTTCCTTAAGGTGAATT
AAGAACAATAGATTTACGCT

Product: phosphate ABC transporter permease PstA

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTLSNPKDLPGLEDSENSQFKPALDTRYRWDRFFEALTWLATIISLVVLATLLFDVLSDGASSLSVDFLTSPPSRKAAKA
GLWPALMGTVWLLCITAAVAFPIGVGSGIFLEEFAPDNLFTRIIEINVGNLAAVPSIVYGLLGLTVFVDIMRPITGGSSV
LAGGLTLALLILPIIIVSTREALRAVPSSLRQGGFALGATRWQVIRDLVFPQSLPGILTGTILALSRAIGETAPILVVGA
AGYIAFAPPLSLEGLQSDFTALPIQIYVWIARAQKSFEPIAAGGIIILLVVLLLMNTTAILLRNKFQNQKL

Sequences:

>Translated_311_residues
MTLSNPKDLPGLEDSENSQFKPALDTRYRWDRFFEALTWLATIISLVVLATLLFDVLSDGASSLSVDFLTSPPSRKAAKA
GLWPALMGTVWLLCITAAVAFPIGVGSGIFLEEFAPDNLFTRIIEINVGNLAAVPSIVYGLLGLTVFVDIMRPITGGSSV
LAGGLTLALLILPIIIVSTREALRAVPSSLRQGGFALGATRWQVIRDLVFPQSLPGILTGTILALSRAIGETAPILVVGA
AGYIAFAPPLSLEGLQSDFTALPIQIYVWIARAQKSFEPIAAGGIIILLVVLLLMNTTAILLRNKFQNQKL
>Mature_310_residues
TLSNPKDLPGLEDSENSQFKPALDTRYRWDRFFEALTWLATIISLVVLATLLFDVLSDGASSLSVDFLTSPPSRKAAKAG
LWPALMGTVWLLCITAAVAFPIGVGSGIFLEEFAPDNLFTRIIEINVGNLAAVPSIVYGLLGLTVFVDIMRPITGGSSVL
AGGLTLALLILPIIIVSTREALRAVPSSLRQGGFALGATRWQVIRDLVFPQSLPGILTGTILALSRAIGETAPILVVGAA
GYIAFAPPLSLEGLQSDFTALPIQIYVWIARAQKSFEPIAAGGIIILLVVLLLMNTTAILLRNKFQNQKL

Specific function: Part of the binding-protein-dependent transport system yqgGHIJK. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0581

COG function: function code P; ABC-type phosphate transport system, permease component

Gene ontology:
GO:0005215: Phosphate ABC transporter, permease protein PstA
GO:0005315: Phosphate ABC transporter, permease protein PstA
GO:0005886: Phosphate ABC transporter, permease protein PstA
GO:0006810: Phosphate ABC transporter, permease protein PstA
GO:0016020: Phosphate ABC transporter, permease protein PstA
GO:0016021: Phosphate ABC transporter, permease protein PstA
GO:0035435: Phosphate ABC transporter, permease protein PstA

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790163, Length=252, Percent_Identity=31.7460317460317, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790164, Length=250, Percent_Identity=30.4, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005672 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33225; Mature: 33093

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSNPKDLPGLEDSENSQFKPALDTRYRWDRFFEALTWLATIISLVVLATLLFDVLSDG
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ASSLSVDFLTSPPSRKAAKAGLWPALMGTVWLLCITAAVAFPIGVGSGIFLEEFAPDNLF
CCCEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHH
TRIIEINVGNLAAVPSIVYGLLGLTVFVDIMRPITGGSSVLAGGLTLALLILPIIIVSTR
HHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
EALRAVPSSLRQGGFALGATRWQVIRDLVFPQSLPGILTGTILALSRAIGETAPILVVGA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
AGYIAFAPPLSLEGLQSDFTALPIQIYVWIARAQKSFEPIAAGGIIILLVVLLLMNTTAI
CCCEEECCCCCHHHHHCCHHHCCHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHH
LLRNKFQNQKL
HHHHHHHCCCC
>Mature Secondary Structure 
TLSNPKDLPGLEDSENSQFKPALDTRYRWDRFFEALTWLATIISLVVLATLLFDVLSDG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ASSLSVDFLTSPPSRKAAKAGLWPALMGTVWLLCITAAVAFPIGVGSGIFLEEFAPDNLF
CCCEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHH
TRIIEINVGNLAAVPSIVYGLLGLTVFVDIMRPITGGSSVLAGGLTLALLILPIIIVSTR
HHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
EALRAVPSSLRQGGFALGATRWQVIRDLVFPQSLPGILTGTILALSRAIGETAPILVVGA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
AGYIAFAPPLSLEGLQSDFTALPIQIYVWIARAQKSFEPIAAGGIIILLVVLLLMNTTAI
CCCEEECCCCCHHHHHCCHHHCCHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHH
LLRNKFQNQKL
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8760913; 8969508; 9384377 [H]