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The map label for this gene is rnfC [H]

Identifier: 153937803

GI number: 153937803

Start: 2461270

End: 2462388

Strand: Reverse

Name: rnfC [H]

Synonym: CLC_2333

Alternate gene names: 153937803

Gene position: 2462388-2461270 (Counterclockwise)

Preceding gene: 153936829

Following gene: 153935144

Centisome position: 65.48

GC content: 34.14

Gene sequence:

>1119_bases
ATGGAGAAGTTATACAAATTCTCCCTTAAAATGCACGTAGGTGCACCAGATGTATCTTGTGTAAAAGAAGGACAAAAAGT
GAAGAGAGGTGAATGCATAGCAGAGCCAAACGGACTTGGTGCTAAAATACATACTAGTGTATCCGGTGTAGTAGAAAAGA
TTACAGATAAGGAAATTATAATAAAAGCGGATGAGACTCAAACAAAAGAATTCGTTAAAATAAAAAAATGCGATAATTTA
GTAGATACTGTATTTGAAGCAGGGATAGTCGGAGCTGGTGGAGCAGGTTTCCCAACTCACATAAAGCTAAAGGCAGATAA
CAAAGATGGTTATATTATAGCAAACTGTGTAGAATGTGAACCAGCACTTCATCACAATATGAAAGTTATAGAAGAAACAC
CGGAATTAATAATAAATGGTATTAAGTATGCTATGAAGGCTACTAATTCAAAAAAAGGATACATAGCAATAAAATCAAAA
CATGAAAAAGCAGTAAGAGTATTAGAAGAAGCATTAAAGAATGTAAGTGATATAGAAATAAAATTGCTTAAAGACTTATA
CCCAATGGGTGAAGAAAGAGCTATAATCAATGCAATATTTGATAAATGGTTAGATGTAACTGAACTTCCTATAGCAGCAA
AATGTATTGTAATGAATGCTGAAACTTTGGCAAATATTACAAGAGCTGTTGAAGAAGGAAAACCAGTTATAGATAAGGAT
ATAACAGTAATAGGAAAACTAAAATCAGGTAATAAACCAAATGTATTCCTACAAGTTCCAGTGGGAACGCCAGTAAAAGA
TTTAATAGAAAAATCTGGTGGTATAGATGGTGAATATGGTGAACTAGTAATAGGTGGACCATACACTGGAAAGGCTGGAG
ATATAGAAAAGGATGCTGTTACTAAAATATCTGGTGGAGCAATAGTTACAATACCATTACCAGAATATAAAGGCCCATTA
GGACTACTAGTATGTGCCTGTGGAGCTAACGAAGAAAGATTAAAAGATGTAGCAACTAAAATGAATGCACAAATTGCAGG
GGTAGTTGATTGCAAAAACATAGAATATCCAAAGGGTAAAGGAAATGGACCAGGAAAATGCAAAACACCTGGTGAATGA

Upstream 100 bases:

>100_bases
ATAGCTATAACCTAGTTTTATAGAAATTGTTTATTTTTTTAACAAAGTTGGCATAGTTTATGCTTTAAAATATATATGAC
AGTGATTTGGAGGTGGGTTT

Downstream 100 bases:

>100_bases
CCAGGCCAAGCGGCTGCAGTTATGCAGCTTAAAAAAGATGGAGCAGAAAGAATATTAATATCAAATTGTAATGATTGTTC
AAACACAGTTATGCAAATAG

Product: NADH dehydrogenase family protein

Products: NA

Alternate protein names: Nitrogen fixation protein rnfC [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MEKLYKFSLKMHVGAPDVSCVKEGQKVKRGECIAEPNGLGAKIHTSVSGVVEKITDKEIIIKADETQTKEFVKIKKCDNL
VDTVFEAGIVGAGGAGFPTHIKLKADNKDGYIIANCVECEPALHHNMKVIEETPELIINGIKYAMKATNSKKGYIAIKSK
HEKAVRVLEEALKNVSDIEIKLLKDLYPMGEERAIINAIFDKWLDVTELPIAAKCIVMNAETLANITRAVEEGKPVIDKD
ITVIGKLKSGNKPNVFLQVPVGTPVKDLIEKSGGIDGEYGELVIGGPYTGKAGDIEKDAVTKISGGAIVTIPLPEYKGPL
GLLVCACGANEERLKDVATKMNAQIAGVVDCKNIEYPKGKGNGPGKCKTPGE

Sequences:

>Translated_372_residues
MEKLYKFSLKMHVGAPDVSCVKEGQKVKRGECIAEPNGLGAKIHTSVSGVVEKITDKEIIIKADETQTKEFVKIKKCDNL
VDTVFEAGIVGAGGAGFPTHIKLKADNKDGYIIANCVECEPALHHNMKVIEETPELIINGIKYAMKATNSKKGYIAIKSK
HEKAVRVLEEALKNVSDIEIKLLKDLYPMGEERAIINAIFDKWLDVTELPIAAKCIVMNAETLANITRAVEEGKPVIDKD
ITVIGKLKSGNKPNVFLQVPVGTPVKDLIEKSGGIDGEYGELVIGGPYTGKAGDIEKDAVTKISGGAIVTIPLPEYKGPL
GLLVCACGANEERLKDVATKMNAQIAGVVDCKNIEYPKGKGNGPGKCKTPGE
>Mature_372_residues
MEKLYKFSLKMHVGAPDVSCVKEGQKVKRGECIAEPNGLGAKIHTSVSGVVEKITDKEIIIKADETQTKEFVKIKKCDNL
VDTVFEAGIVGAGGAGFPTHIKLKADNKDGYIIANCVECEPALHHNMKVIEETPELIINGIKYAMKATNSKKGYIAIKSK
HEKAVRVLEEALKNVSDIEIKLLKDLYPMGEERAIINAIFDKWLDVTELPIAAKCIVMNAETLANITRAVEEGKPVIDKD
ITVIGKLKSGNKPNVFLQVPVGTPVKDLIEKSGGIDGEYGELVIGGPYTGKAGDIEKDAVTKISGGAIVTIPLPEYKGPL
GLLVCACGANEERLKDVATKMNAQIAGVVDCKNIEYPKGKGNGPGKCKTPGE

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Stabilizes rnfB [H]

COG id: COG4656

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfC

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787916, Length=314, Percent_Identity=24.5222929936306, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1788620, Length=236, Percent_Identity=24.5762711864407, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010208
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 39976; Mature: 39976

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLYKFSLKMHVGAPDVSCVKEGQKVKRGECIAEPNGLGAKIHTSVSGVVEKITDKEII
CCCCEEEEEEEEECCCCHHHHHCCCHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCCCEEE
IKADETQTKEFVKIKKCDNLVDTVFEAGIVGAGGAGFPTHIKLKADNKDGYIIANCVECE
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCC
PALHHNMKVIEETPELIINGIKYAMKATNSKKGYIAIKSKHEKAVRVLEEALKNVSDIEI
HHHHCCCHHHHHCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHH
KLLKDLYPMGEERAIINAIFDKWLDVTELPIAAKCIVMNAETLANITRAVEEGKPVIDKD
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCC
ITVIGKLKSGNKPNVFLQVPVGTPVKDLIEKSGGIDGEYGELVIGGPYTGKAGDIEKDAV
EEEEEEECCCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHH
TKISGGAIVTIPLPEYKGPLGLLVCACGANEERLKDVATKMNAQIAGVVDCKNIEYPKGK
EEECCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCCC
GNGPGKCKTPGE
CCCCCCCCCCCC
>Mature Secondary Structure
MEKLYKFSLKMHVGAPDVSCVKEGQKVKRGECIAEPNGLGAKIHTSVSGVVEKITDKEII
CCCCEEEEEEEEECCCCHHHHHCCCHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCCCEEE
IKADETQTKEFVKIKKCDNLVDTVFEAGIVGAGGAGFPTHIKLKADNKDGYIIANCVECE
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCC
PALHHNMKVIEETPELIINGIKYAMKATNSKKGYIAIKSKHEKAVRVLEEALKNVSDIEI
HHHHCCCHHHHHCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHH
KLLKDLYPMGEERAIINAIFDKWLDVTELPIAAKCIVMNAETLANITRAVEEGKPVIDKD
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCC
ITVIGKLKSGNKPNVFLQVPVGTPVKDLIEKSGGIDGEYGELVIGGPYTGKAGDIEKDAV
EEEEEEECCCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHH
TKISGGAIVTIPLPEYKGPLGLLVCACGANEERLKDVATKMNAQIAGVVDCKNIEYPKGK
EEECCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCCC
GNGPGKCKTPGE
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S clusters [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268; 9154934 [H]