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The map label for this gene is potD [H]

Identifier: 153936910

GI number: 153936910

Start: 635963

End: 637804

Strand: Direct

Name: potD [H]

Synonym: CLC_0620

Alternate gene names: 153936910

Gene position: 635963-637804 (Clockwise)

Preceding gene: 153935952

Following gene: 153934521

Centisome position: 16.91

GC content: 30.08

Gene sequence:

>1842_bases
ATGAAGAGCAATAAAGGTTTATTAAGTAAAATATATGCAACTTTAGTTTATGTTTTTTTATATTTGCCAATCTTTGTTTT
AGTAGTATTTTCTTTTAATAAGTCTAAACTAAATGCTACCTTTACAGGCTTTACACTAGATTGGTACAAAAATTTAATAA
ATAATGTTCAAATATTAGAAGCATTAAAAAACAGCTTAATAATAGCTTTTATAAGTACTTTTTTCGCAGTAATTATAGGA
ACTTTAGCTGCTATAGGTATGTATAGATATAAATTTAAGGGGAAAAGGGCTATGGAGGGACTGCTTTATATCCCAGTAGT
TATCCCTGAAATAGTTATGGGGATATCTATGCTTGCCTTTTTCTCTTCCCTTAATTTGCCTGCAGGACTTATAACTTTAA
TACTTGCTCACATAACTTTTTGTATTTCTTACGTTATTATAGTAGTTAGAGCTAGACTAGATGGGTTTGATGCTGCACTA
GAAGAAGCAGCTCAAGATTTAGGTGCTACACCTTGGCAAACTTTAACGAAGGTTACACTACCTGTAATATCACCAGGAAT
TATATCAGGAGCACTGCTTGCTTTTACACTATCTTTAGACGATGTTATTATAAGTTTTTTTGCGGCAGGACCAGATAGCA
ATACTTTACCACTTAAAATATTTTCTATGGTTAAGTTTGGTGTAACCCCTGAAATAAATGCACTATCCACTGTGATGATG
GTATTTACTTTAAGTATGGTAGTTATAGCTGAAGGTATAAGAAGAAATATGCTTAAAAATAAAAAAGTAAAAAAGACACT
ATCCTTTATAGTAATATTATTAATGGTTACTGGGGTTGGCTTTACTATTTTTGGTAATACAGCTAAAACAGAAAAGCAAG
TGTTAAATATATTTAACTGGTCAGAATTTTTACCACAGTCTGTTATTGAACAGTTTGAAAAAGAATACAATGTAAAGGTA
AATTATTCTACTTTTTCCTCTAATGAAGAAATGCTTGCAAAGTTAATGGGAGGAAATGTGCCCTATGATTTAGTAGTTAC
CAGTGATTATGCTATTGAAATAATGACTAAACAAAAATTAATTCAACCAATAGACAAAAATAATGTTCCAAACTTAGCTA
ATATAGATAAAAATGTACTTGATTTAGCTTTTGATCCTAAAAATACTTATAGCTTACCTTATATGTGGGGAGGCAACAAT
ATAGTAATAGATAAGACTAAAATAACTAAAAAAATTACTTCTTTTGATGATCTGTGGGACAGTGAATTTAAAAATTCTAT
GGTAATTTTGGATGATCCAAGGGTGATGATAGGATTAGCTCTACAAAAGAATGGATATTCTATAAATTCAAAAAATCCAA
AGGAACTTCAAAAGGCTAAAGAGGATTTAATAAAATTGATGCCAAATGTAAAAGCATTTGATAGTGAATCTCCAAAGACT
TTACTTATAAATGGAGAAGCTTCTATAGGATATGTATGGGGAACGGAAGCATATCTTGCTAAACTTGAAAATCCTAATTT
AGAAGTAGTTTTAACAAAAGAGGGAGTAATTCCTCAATATGATAACTTTGTAATACCTAAAAAGGCTAAAAATAAAAAAT
TAGCAGAGGAATTTATAAACTTCATATATAAACCTGAAATAAGTGCTCAAGTGTCTGAGGAATTTCCTTATGCAAATCCA
AACAAGGCAGCATACCCTTTAATGGATAAAGATAAATTAAATGATATAGCTGTTTATCCACCAAGAGAAGCGGTAGAAGG
AAATGAGCTTATTAAGGATGTAGGGGAAACTACAAAATTATACGATGACATATGGACTGAAATTAAAAATAGTAAAAGAT
AA

Upstream 100 bases:

>100_bases
CGGCTATAGCAGTAATATTAATTATTTTAATGCTTATAGCTATAAGTTTCTATTTTAAAGGAAATAAACCAAAGAAGGGA
ATAAAGGAGGTTAACTAAAG

Downstream 100 bases:

>100_bases
CACAAGATTTAAAAATTTTCTAACCAGGTGAGAAATTATCCGTGATTTCACACCTGGTTTCTCATGTTTTGTTAGAAATA
ATAACTGGAAGTATTTATCT

Product: putative spermidine/putrescine ABC transporter, permease protein/spermidine/putrescine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MKSNKGLLSKIYATLVYVFLYLPIFVLVVFSFNKSKLNATFTGFTLDWYKNLINNVQILEALKNSLIIAFISTFFAVIIG
TLAAIGMYRYKFKGKRAMEGLLYIPVVIPEIVMGISMLAFFSSLNLPAGLITLILAHITFCISYVIIVVRARLDGFDAAL
EEAAQDLGATPWQTLTKVTLPVISPGIISGALLAFTLSLDDVIISFFAAGPDSNTLPLKIFSMVKFGVTPEINALSTVMM
VFTLSMVVIAEGIRRNMLKNKKVKKTLSFIVILLMVTGVGFTIFGNTAKTEKQVLNIFNWSEFLPQSVIEQFEKEYNVKV
NYSTFSSNEEMLAKLMGGNVPYDLVVTSDYAIEIMTKQKLIQPIDKNNVPNLANIDKNVLDLAFDPKNTYSLPYMWGGNN
IVIDKTKITKKITSFDDLWDSEFKNSMVILDDPRVMIGLALQKNGYSINSKNPKELQKAKEDLIKLMPNVKAFDSESPKT
LLINGEASIGYVWGTEAYLAKLENPNLEVVLTKEGVIPQYDNFVIPKKAKNKKLAEEFINFIYKPEISAQVSEEFPYANP
NKAAYPLMDKDKLNDIAVYPPREAVEGNELIKDVGETTKLYDDIWTEIKNSKR

Sequences:

>Translated_613_residues
MKSNKGLLSKIYATLVYVFLYLPIFVLVVFSFNKSKLNATFTGFTLDWYKNLINNVQILEALKNSLIIAFISTFFAVIIG
TLAAIGMYRYKFKGKRAMEGLLYIPVVIPEIVMGISMLAFFSSLNLPAGLITLILAHITFCISYVIIVVRARLDGFDAAL
EEAAQDLGATPWQTLTKVTLPVISPGIISGALLAFTLSLDDVIISFFAAGPDSNTLPLKIFSMVKFGVTPEINALSTVMM
VFTLSMVVIAEGIRRNMLKNKKVKKTLSFIVILLMVTGVGFTIFGNTAKTEKQVLNIFNWSEFLPQSVIEQFEKEYNVKV
NYSTFSSNEEMLAKLMGGNVPYDLVVTSDYAIEIMTKQKLIQPIDKNNVPNLANIDKNVLDLAFDPKNTYSLPYMWGGNN
IVIDKTKITKKITSFDDLWDSEFKNSMVILDDPRVMIGLALQKNGYSINSKNPKELQKAKEDLIKLMPNVKAFDSESPKT
LLINGEASIGYVWGTEAYLAKLENPNLEVVLTKEGVIPQYDNFVIPKKAKNKKLAEEFINFIYKPEISAQVSEEFPYANP
NKAAYPLMDKDKLNDIAVYPPREAVEGNELIKDVGETTKLYDDIWTEIKNSKR
>Mature_613_residues
MKSNKGLLSKIYATLVYVFLYLPIFVLVVFSFNKSKLNATFTGFTLDWYKNLINNVQILEALKNSLIIAFISTFFAVIIG
TLAAIGMYRYKFKGKRAMEGLLYIPVVIPEIVMGISMLAFFSSLNLPAGLITLILAHITFCISYVIIVVRARLDGFDAAL
EEAAQDLGATPWQTLTKVTLPVISPGIISGALLAFTLSLDDVIISFFAAGPDSNTLPLKIFSMVKFGVTPEINALSTVMM
VFTLSMVVIAEGIRRNMLKNKKVKKTLSFIVILLMVTGVGFTIFGNTAKTEKQVLNIFNWSEFLPQSVIEQFEKEYNVKV
NYSTFSSNEEMLAKLMGGNVPYDLVVTSDYAIEIMTKQKLIQPIDKNNVPNLANIDKNVLDLAFDPKNTYSLPYMWGGNN
IVIDKTKITKKITSFDDLWDSEFKNSMVILDDPRVMIGLALQKNGYSINSKNPKELQKAKEDLIKLMPNVKAFDSESPKT
LLINGEASIGYVWGTEAYLAKLENPNLEVVLTKEGVIPQYDNFVIPKKAKNKKLAEEFINFIYKPEISAQVSEEFPYANP
NKAAYPLMDKDKLNDIAVYPPREAVEGNELIKDVGETTKLYDDIWTEIKNSKR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=324, Percent_Identity=39.8148148148148, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI1787368, Length=254, Percent_Identity=44.8818897637795, Blast_Score=222, Evalue=7e-59,
Organism=Escherichia coli, GI1787081, Length=233, Percent_Identity=43.7768240343348, Blast_Score=185, Evalue=8e-48,
Organism=Escherichia coli, GI1787078, Length=341, Percent_Identity=32.5513196480938, Blast_Score=166, Evalue=6e-42,
Organism=Escherichia coli, GI1787714, Length=205, Percent_Identity=39.0243902439024, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI1788764, Length=179, Percent_Identity=30.7262569832402, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 68663; Mature: 68663

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSNKGLLSKIYATLVYVFLYLPIFVLVVFSFNKSKLNATFTGFTLDWYKNLINNVQILE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECEEHHHHHHHHHHHHHHH
ALKNSLIIAFISTFFAVIIGTLAAIGMYRYKFKGKRAMEGLLYIPVVIPEIVMGISMLAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEHHHHHHHHHHHHHHHHH
FSSLNLPAGLITLILAHITFCISYVIIVVRARLDGFDAALEEAAQDLGATPWQTLTKVTL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHH
PVISPGIISGALLAFTLSLDDVIISFFAAGPDSNTLPLKIFSMVKFGVTPEINALSTVMM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
VFTLSMVVIAEGIRRNMLKNKKVKKTLSFIVILLMVTGVGFTIFGNTAKTEKQVLNIFNW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHH
SEFLPQSVIEQFEKEYNVKVNYSTFSSNEEMLAKLMGGNVPYDLVVTSDYAIEIMTKQKL
HHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCCEEEEEECCCEEEEEHHHHH
IQPIDKNNVPNLANIDKNVLDLAFDPKNTYSLPYMWGGNNIVIDKTKITKKITSFDDLWD
HCCCCCCCCCCHHHCCCHHHEEEECCCCCEECCEEECCCEEEEECHHHHHHHHHHHHHHH
SEFKNSMVILDDPRVMIGLALQKNGYSINSKNPKELQKAKEDLIKLMPNVKAFDSESPKT
HHHCCCEEEEECCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCE
LLINGEASIGYVWGTEAYLAKLENPNLEVVLTKEGVIPQYDNFVIPKKAKNKKLAEEFIN
EEEECCCCEEEEECCHHHHHHCCCCCEEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHH
FIYKPEISAQVSEEFPYANPNKAAYPLMDKDKLNDIAVYPPREAVEGNELIKDVGETTKL
HHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHCCHHHHHHHHHHHHHH
YDDIWTEIKNSKR
HHHHHHHHHCCCC
>Mature Secondary Structure
MKSNKGLLSKIYATLVYVFLYLPIFVLVVFSFNKSKLNATFTGFTLDWYKNLINNVQILE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECEEHHHHHHHHHHHHHHH
ALKNSLIIAFISTFFAVIIGTLAAIGMYRYKFKGKRAMEGLLYIPVVIPEIVMGISMLAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEHHHHHHHHHHHHHHHHH
FSSLNLPAGLITLILAHITFCISYVIIVVRARLDGFDAALEEAAQDLGATPWQTLTKVTL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHH
PVISPGIISGALLAFTLSLDDVIISFFAAGPDSNTLPLKIFSMVKFGVTPEINALSTVMM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
VFTLSMVVIAEGIRRNMLKNKKVKKTLSFIVILLMVTGVGFTIFGNTAKTEKQVLNIFNW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHH
SEFLPQSVIEQFEKEYNVKVNYSTFSSNEEMLAKLMGGNVPYDLVVTSDYAIEIMTKQKL
HHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCCCEEEEEECCCEEEEEHHHHH
IQPIDKNNVPNLANIDKNVLDLAFDPKNTYSLPYMWGGNNIVIDKTKITKKITSFDDLWD
HCCCCCCCCCCHHHCCCHHHEEEECCCCCEECCEEECCCEEEEECHHHHHHHHHHHHHHH
SEFKNSMVILDDPRVMIGLALQKNGYSINSKNPKELQKAKEDLIKLMPNVKAFDSESPKT
HHHCCCEEEEECCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCE
LLINGEASIGYVWGTEAYLAKLENPNLEVVLTKEGVIPQYDNFVIPKKAKNKKLAEEFIN
EEEECCCCEEEEECCHHHHHHCCCCCEEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHH
FIYKPEISAQVSEEFPYANPNKAAYPLMDKDKLNDIAVYPPREAVEGNELIKDVGETTKL
HHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHCCHHHHHHHHHHHHHH
YDDIWTEIKNSKR
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]