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The map label for this gene is ackA

Identifier: 153936264

GI number: 153936264

Start: 2435550

End: 2436743

Strand: Reverse

Name: ackA

Synonym: CLC_2308

Alternate gene names: 153936264

Gene position: 2436743-2435550 (Counterclockwise)

Preceding gene: 153935755

Following gene: 153935014

Centisome position: 64.8

GC content: 33.33

Gene sequence:

>1194_bases
ATGAAAATATTAGTAGTTAACTGCGGAAGTTCGTCATTAAAGTATCAATTAATAGATATGACTTCTGAAGAAGCTTTAGC
AAAGGGATTAGTAGAGAGAATAGGAATAGAAGGCTCTATATTGACTCAAAAGGTTAATGGAGAAAAATATATAATAGAAG
AACCTATGAAAGATCATAAGAAAGCTATAGAGTTAGTTTTAAAAGCATTAGTAGACAAAGAGCATGGTGTAATATCTGAT
ATGTCAGAAATAGCAGCAGTAGGACATAGAGTAGTTCACGGTGGAGAAAAATATGCTAGCTCAGTACTTATAAATGATGA
GGTTATGAAGGCTTTAGAAGATTGTGTTAAATTAGCACCACTTCATAATCCACCAAACATAATAGGAATAAATGCTTGTA
GAGAACTTATGCCTAAAACTCCAATGGTAGCAGTATTTGATACAGCATTCCATCAAACATTACCAGACTATGCATATATG
TATCCATTACCATATGAATTATATGAACAAAATGGTATAAGAAAATATGGTTTCCATGGAACATCACATAGATATGTATC
TAGTGTAGCGTCAGAGATGATGGGAAAAGATCTTAAAGATCTTAAAGTAATAACTTGTCACTTAGGAAATGGAGCAAGTT
TATGTGCTGTAAAAGAAGGAAAGTCAGTAGAAACCTCTATGGGATTCACGCCACTTGCAGGATTGGCTATGGGAACTAGA
TGCGGAGATATAGATCCAGCAATACTTCTTTTCATGGAAAGAGAATTAAAGATGTCACCAGATGAAGTAGATACCGTTAT
AAATAAAAAATCAGGTGTATTAGGAATATCAGGAGTAAGTAGTGACTTCAGAGACATAGAAGGTGCCGCAGAAGAAGGAA
ATAAAAGAGCTAAGCTAGCTTTAGATGTATATCATTACACAGTAAGACAGACAATCGGTGCTTATACAGCAGTATTAAAT
GGTGTAGATGCTATAGTATTTACAGCAGGACTAGGAGAAAATTCAGCAGCAAGCAGAGAAGAAATTTTAAATGGATTAGA
ATACTTAGGTATTAAGATAGATGCCGAAAAGAATAAACAAAGAGGAAAACAAATAGAAATAAGTACTGAAGATTCAAAGG
TAAAGGTATTCGTTATACCAACTGATGAAGAATTAATGATAGCAAGAGATACTAAAGAAATAACAGTTAAATAA

Upstream 100 bases:

>100_bases
TTTTAGATGATAAATTAATGTATTAATATCACGAATAAAAAAATAGATTTGCTTATAATAAAATTTTATATAAAACATAT
AGATGAAGGGTGGAAAATTA

Downstream 100 bases:

>100_bases
TATGTAATATAAGTTATAAATATAGATATAATAACTTTGATTTACTCATAAAATACTTGACTTTTTAATATGCCCTTTAT
ATAATAAATTGTGGTTGCTA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKILVVNCGSSSLKYQLIDMTSEEALAKGLVERIGIEGSILTQKVNGEKYIIEEPMKDHKKAIELVLKALVDKEHGVISD
MSEIAAVGHRVVHGGEKYASSVLINDEVMKALEDCVKLAPLHNPPNIIGINACRELMPKTPMVAVFDTAFHQTLPDYAYM
YPLPYELYEQNGIRKYGFHGTSHRYVSSVASEMMGKDLKDLKVITCHLGNGASLCAVKEGKSVETSMGFTPLAGLAMGTR
CGDIDPAILLFMERELKMSPDEVDTVINKKSGVLGISGVSSDFRDIEGAAEEGNKRAKLALDVYHYTVRQTIGAYTAVLN
GVDAIVFTAGLGENSAASREEILNGLEYLGIKIDAEKNKQRGKQIEISTEDSKVKVFVIPTDEELMIARDTKEITVK

Sequences:

>Translated_397_residues
MKILVVNCGSSSLKYQLIDMTSEEALAKGLVERIGIEGSILTQKVNGEKYIIEEPMKDHKKAIELVLKALVDKEHGVISD
MSEIAAVGHRVVHGGEKYASSVLINDEVMKALEDCVKLAPLHNPPNIIGINACRELMPKTPMVAVFDTAFHQTLPDYAYM
YPLPYELYEQNGIRKYGFHGTSHRYVSSVASEMMGKDLKDLKVITCHLGNGASLCAVKEGKSVETSMGFTPLAGLAMGTR
CGDIDPAILLFMERELKMSPDEVDTVINKKSGVLGISGVSSDFRDIEGAAEEGNKRAKLALDVYHYTVRQTIGAYTAVLN
GVDAIVFTAGLGENSAASREEILNGLEYLGIKIDAEKNKQRGKQIEISTEDSKVKVFVIPTDEELMIARDTKEITVK
>Mature_397_residues
MKILVVNCGSSSLKYQLIDMTSEEALAKGLVERIGIEGSILTQKVNGEKYIIEEPMKDHKKAIELVLKALVDKEHGVISD
MSEIAAVGHRVVHGGEKYASSVLINDEVMKALEDCVKLAPLHNPPNIIGINACRELMPKTPMVAVFDTAFHQTLPDYAYM
YPLPYELYEQNGIRKYGFHGTSHRYVSSVASEMMGKDLKDLKVITCHLGNGASLCAVKEGKSVETSMGFTPLAGLAMGTR
CGDIDPAILLFMERELKMSPDEVDTVINKKSGVLGISGVSSDFRDIEGAAEEGNKRAKLALDVYHYTVRQTIGAYTAVLN
GVDAIVFTAGLGENSAASREEILNGLEYLGIKIDAEKNKQRGKQIEISTEDSKVKVFVIPTDEELMIARDTKEITVK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=403, Percent_Identity=45.1612903225806, Blast_Score=341, Evalue=5e-95,
Organism=Escherichia coli, GI145693184, Length=393, Percent_Identity=43.2569974554707, Blast_Score=324, Evalue=7e-90,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_CLOB1 (A7FW24)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384635.1
- ProteinModelPortal:   A7FW24
- SMR:   A7FW24
- STRING:   A7FW24
- GeneID:   5396212
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2324
- eggNOG:   COG0282
- HOGENOM:   HBG285863
- OMA:   SSDFRDV
- ProtClustDB:   PRK00180
- BioCyc:   CBOT441770:CLB_2324-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43431; Mature: 43431

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVVNCGSSSLKYQLIDMTSEEALAKGLVERIGIEGSILTQKVNGEKYIIEEPMKDHK
CEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCHHHHH
KAIELVLKALVDKEHGVISDMSEIAAVGHRVVHGGEKYASSVLINDEVMKALEDCVKLAP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHEEEECHHHHHHHHHHHHHCC
LHNPPNIIGINACRELMPKTPMVAVFDTAFHQTLPDYAYMYPLPYELYEQNGIRKYGFHG
CCCCCCEEEHHHHHHHCCCCCEEEEEHHHHHHHCCCCEEECCCCHHHHHCCCCEEECCCC
TSHRYVSSVASEMMGKDLKDLKVITCHLGNGASLCAVKEGKSVETSMGFTPLAGLAMGTR
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHCCC
CGDIDPAILLFMERELKMSPDEVDTVINKKSGVLGISGVSSDFRDIEGAAEEGNKRAKLA
CCCCCHHHHHEEHHHCCCCHHHHHHHHCCCCCEEEECCCCCHHHHHCCHHHCCCCEEEEE
LDVYHYTVRQTIGAYTAVLNGVDAIVFTAGLGENSAASREEILNGLEYLGIKIDAEKNKQ
EHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCHHHHEEEEEECCCCCC
RGKQIEISTEDSKVKVFVIPTDEELMIARDTKEITVK
CCCEEEEECCCCEEEEEEEECCCCEEEEECCCEEEEC
>Mature Secondary Structure
MKILVVNCGSSSLKYQLIDMTSEEALAKGLVERIGIEGSILTQKVNGEKYIIEEPMKDHK
CEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCHHHHH
KAIELVLKALVDKEHGVISDMSEIAAVGHRVVHGGEKYASSVLINDEVMKALEDCVKLAP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHEEEECHHHHHHHHHHHHHCC
LHNPPNIIGINACRELMPKTPMVAVFDTAFHQTLPDYAYMYPLPYELYEQNGIRKYGFHG
CCCCCCEEEHHHHHHHCCCCCEEEEEHHHHHHHCCCCEEECCCCHHHHHCCCCEEECCCC
TSHRYVSSVASEMMGKDLKDLKVITCHLGNGASLCAVKEGKSVETSMGFTPLAGLAMGTR
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHCCC
CGDIDPAILLFMERELKMSPDEVDTVINKKSGVLGISGVSSDFRDIEGAAEEGNKRAKLA
CCCCCHHHHHEEHHHCCCCHHHHHHHHCCCCCEEEECCCCCHHHHHCCHHHCCCCEEEEE
LDVYHYTVRQTIGAYTAVLNGVDAIVFTAGLGENSAASREEILNGLEYLGIKIDAEKNKQ
EHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCHHHHEEEEEECCCCCC
RGKQIEISTEDSKVKVFVIPTDEELMIARDTKEITVK
CCCEEEEECCCCEEEEEEEECCCCEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA